Tissue-specific changes in protein synthesis rates in vivo during anoxia in crucian carp

1996 ◽  
Vol 271 (4) ◽  
pp. R897-R904 ◽  
Author(s):  
R. W. Smith ◽  
D. F. Houlihan ◽  
G. E. Nilsson ◽  
J. G. Brechin
Keyword(s):  
Protein Synthesis ◽  
White Muscle ◽  
Crucian Carp ◽  
Translational Efficiency ◽  
Neural Function ◽  
Tissue Specific ◽  
Significant Mechanism ◽  
Brain Protein Synthesis ◽  
The Brain

Mechanisms of anoxia tolerance were investigated in crucian carp. Rates of protein synthesis were calculated in selected tissues of normoxic and anoxic animals. Exposure to 48 h of anoxia resulted in a significant reduction in protein synthesis in the liver (> 95%), heart (53%), and red and white muscle (52 and 56%, respectively), whereas brain protein synthesis rates were unaffected. Seven days of anoxia produced similar results. After 24 h of recovery from a 48-h anoxic period, protein synthesis rates had virtually returned to normoxic values. The effect of anoxia on the amount of RNA (relative to protein) varied depending on the tissue and also the length of exposure (except in the brain, where it was consistently reduced). However, the effect on RNA translational efficiency was purely tissue specific (i.e., independent of exposure time) and was unaffected in the heart, reduced in the liver and red and white muscle, and increased in the brain. Downregulation of protein synthesis on a tissue-specific basis appears to be a significant mechanism for energy conservation as well as maintaining neural function, thus promoting survival during anoxia.

Tissue-specific changes in RNA synthesis in vivo during anoxia in crucian carp

1999 ◽  
Vol 277 (3) ◽  
pp. R690-R697 ◽  
Author(s):  
Richard W. Smith ◽  
Dominic F. Houlihan ◽  
Göran E. Nilsson ◽  
Julie Alexandre
Keyword(s):  
Protein Synthesis ◽  
Rna Synthesis ◽  
Crucian Carp ◽  
Synthesis Process ◽  
Synthesis Rate ◽  
Variable Component ◽  
Tissue Specific ◽  
Species Survival ◽  
Energetic Demand

The overall energy budget for protein synthesis (i.e., transcription plus translation) is thought to consist of fixed and variable components, with RNA synthesis accounting for the former and protein synthesis the latter. During anoxia, the downregulation of protein synthesis (i.e., the variable component), to reduce energetic demand, is an important aspect of survival in crucian carp. The present study examines RNA synthesis during anoxia by labeling with [3H]uridine. A novel synthesis rate calculation is presented, which allows for the tissue-specific salvage of uridine, with synthesis rates finally expressed relative to DNA. After 48 h anoxia, the decline (29%) in brain RNA synthesis and increases in the heart and liver (132 and 871%, respectively) support known RNA functions during hypoxic/anoxic survival. This study provides evidence that, in an anoxia-tolerant species, survival mechanisms involving RNA are able to operate because tissue-specific restructuring of the RNA synthesis process enables fixed synthesis costs to be maintained; this may be as vital to survival as exploiting the variable energetic demand of protein synthesis.

scholarly journals Dietary γ-Aminobutyric Acid Affects the Brain Protein Synthesis Rate in Ovariectomized Female Rats

2009 ◽  
Vol 55 (1) ◽  
pp. 75-80 ◽  
Author(s):  
Kazuyo TUJIOKA ◽  
Miho OHSUMI ◽  
Kenji HORIE ◽  
Mujo KIM ◽  
Kazutoshi HAYASE ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Female Rats ◽  
Aminobutyric Acid ◽  
Synthesis Rate ◽  
Protein Synthesis Rate ◽  
Brain Protein ◽  
Brain Protein Synthesis ◽  
The Brain

Regulation of hepatic protein synthesis in chronic inflammation and sepsis

1992 ◽  
Vol 262 (2) ◽  
pp. C445-C452 ◽  
Author(s):  
T. C. Vary ◽  
S. R. Kimball
Keyword(s):  
Protein Synthesis ◽  
Sterile Inflammation ◽  
Chain Elongation ◽  
Synthesis Rate ◽  
Protein Synthesis Rate ◽  
Translational Efficiency ◽  
Liver Protein ◽  
Ribosomal Subunits ◽  
Hepatic Protein Synthesis

The regulation of protein synthesis was determined in livers from control, sterile inflammatory, and septic animals. Total liver protein was increased in both sterile inflammation and sepsis. The rate of protein synthesis in vivo was measured by the incorporation of [3H]phenylalanine into liver proteins in a chronic (5 day) intra-abdominal abscess model. Both sterile inflammation and sepsis increased total hepatic protein synthesis approximately twofold. Perfused liver studies demonstrated that the increased protein synthesis rate in vivo resulted from a stimulation in the synthesis of both secreted and nonsecreted proteins. The total hepatic RNA content was increased 40% only in sterile inflammation, whereas the translational efficiency was increased twofold only in sepsis. The increase in translational efficiency was accompanied by decreases in the amount of free 40S and 60S ribosomal subunits in sepsis. Rates of peptide-chain elongation in vivo were increased 40% in both sterile inflammation and sepsis. These results demonstrate that sepsis induces changes in the regulation of hepatic protein synthesis that are independent of the general inflammatory response. In sterile inflammation, the increase in protein synthesis occurs by a combination of increased capacity and translational efficiency, while in sepsis, the mechanism responsible for accelerated protein synthesis is an increased translational efficiency.

IN VIVO INHIBITION OF RAT BRAIN PROTEIN SYNTHESIS BY d-AMPHETAMINE

1978 ◽  
Vol 31 (1) ◽  
pp. 341-345 ◽  
Author(s):  
L. E. Roel ◽  
M. A. Moskowitz ◽  
D. Rubin ◽  
D. Markovitz ◽  
L. D. Lytle ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Rat Brain ◽  
Brain Protein ◽  
Brain Protein Synthesis

Tissue-specific changes in protein synthesis associated with seasonal metabolic depression and recovery in the north temperate labrid, Tautogolabrus adspersus

2007 ◽  
Vol 293 (1) ◽  
pp. R474-R481 ◽  
Author(s):  
Johanne M. Lewis ◽  
William R. Driedzic
Keyword(s):  
Protein Synthesis ◽  
Water Temperature ◽  
White Muscle ◽  
Recovery Period ◽  
Synthesis Rate ◽  
Protein Synthesis Rate ◽  
Metabolic Depression ◽  
Tissue Specific ◽  
Total Rna ◽  
Rna Content

The tissue-specific changes in protein synthesis were tracked in relation to the seasonal metabolic depression in cunner ( Tautogolabrus adsperus). In vivo protein synthesis rate and total RNA content were determined in liver, white muscle, brain, heart, and gill during periods of normal activity before metabolic depression, entrance into and during winter dormancy, and during the recovery period. The decrease in water temperature from 8°C to 4°C was accompanied by a 55% depression of protein synthesis in liver, brain, and heart and a 66% depression in gill. Protein synthesis in white muscle fell below detectable levels at this temperature. The depression of protein synthesis is an active process (Q10 = 6–21 between 8°C and 4°C) that occurs in advance of the behavioral and physiological depression at the whole animal level. Protein synthesis was maintained at these depressed levels in white muscle, brain, heart, and gill until water temperature returned to 4°C in the spring. Liver underwent a hyperactivation in the synthesis of proteins at 0°C, which may be linked to antifreeze production. During the recovery period, a hyperactivation of protein synthesis occurred in white muscle, which is suggestive of compensatory growth, as well as in heart and liver, which is considered to be linked to increased activity and feeding. Seasonal changes in total RNA content demonstrate the depression of protein synthesis with decreasing temperature to be closely associated with translational capacity, but the stimulation of protein synthesis during recovery appears to be associated with increased translational efficiency.

scholarly journals The effect of elevated plasma phenylalanine levels on protein synthesis rates in adult rat brain

1994 ◽  
Vol 302 (2) ◽  
pp. 601-610 ◽  
Author(s):  
D S Dunlop ◽  
X R Yang ◽  
A Lajtha
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Free Amino ◽  
Specific Activity ◽  
Brain Protein ◽  
Free Amino Acid Pool ◽  
Plasma Phenylalanine ◽  
Specific Activities ◽  
Brain Protein Synthesis ◽  
The Brain

Increasing the plasma phenylalanine concentration to levels as high as 0.560-0.870 mM (over ten times normal levels) had no detectable effect on the rate of brain protein synthesis in adult rats. The average rates for 7-week-old rats were: valine, 0.58 +/- 0.05%/h, phenylalanine, 0.59 +/- 0.06%/h, and tyrosine, 0.60 +/- 0.09%/h, or 0.59 +/- 0.06%/h overall. Synthesis rates calculated on the basis of the specific activity of the tRNA-bound amino acid were slightly lower (4% lower for phenylalanine) than those based on the brain free amino acid pool. Similarly, the specific activities of valine and phenylalanine in microdialysis fluid from striatum were practically the same as those in the brain free amino acid pool. Thus the specific activities of the valine and phenylalanine brain free pools are good measures of the precursor specific activity for protein synthesis. In any event, synthesis rates, whether based on the specific activities of the amino acids in the brain free pool or those bound to tRNA, were unaffected by elevated levels of plasma phenylalanine. Brain protein synthesis rates measured after the administration of quite large doses of phenylalanine (> 1.5 mumol/g) or valine (15 mumol/g) were in agreement (0.62 +/- 0.01 and 0.65 +/- 0.01%/h respectively) with the rates determined with infusions of trace amounts of amino acids. Thus the technique of stabilizing precursor-specific activity, and pushing values in the brain close to those of the plasma, by the administration of large quantities of precursor, appears to be valid.

scholarly journals Comparison of the Effects of Ornithine and Arginine on the Brain Protein Synthesis Rate in Young Rats

2015 ◽  
Vol 61 (5) ◽  
pp. 417-421 ◽  
Author(s):  
Shoko SUZUMURA ◽  
Kazuyo TUJIOKA ◽  
Takashi YAMADA ◽  
Hidehiko YOKOGOSHI ◽  
Saori AKIDUKI ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Synthesis Rate ◽  
Protein Synthesis Rate ◽  
Brain Protein ◽  
Young Rats ◽  
Brain Protein Synthesis ◽  
The Brain
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