Tissue-specific changes in RNA synthesis in vivo during anoxia in crucian carp

1999 ◽  
Vol 277 (3) ◽  
pp. R690-R697 ◽  
Author(s):  
Richard W. Smith ◽  
Dominic F. Houlihan ◽  
Göran E. Nilsson ◽  
Julie Alexandre
Keyword(s):  
Protein Synthesis ◽  
Rna Synthesis ◽  
Crucian Carp ◽  
Synthesis Process ◽  
Synthesis Rate ◽  
Variable Component ◽  
Tissue Specific ◽  
Species Survival ◽  
Energetic Demand

The overall energy budget for protein synthesis (i.e., transcription plus translation) is thought to consist of fixed and variable components, with RNA synthesis accounting for the former and protein synthesis the latter. During anoxia, the downregulation of protein synthesis (i.e., the variable component), to reduce energetic demand, is an important aspect of survival in crucian carp. The present study examines RNA synthesis during anoxia by labeling with [3H]uridine. A novel synthesis rate calculation is presented, which allows for the tissue-specific salvage of uridine, with synthesis rates finally expressed relative to DNA. After 48 h anoxia, the decline (29%) in brain RNA synthesis and increases in the heart and liver (132 and 871%, respectively) support known RNA functions during hypoxic/anoxic survival. This study provides evidence that, in an anoxia-tolerant species, survival mechanisms involving RNA are able to operate because tissue-specific restructuring of the RNA synthesis process enables fixed synthesis costs to be maintained; this may be as vital to survival as exploiting the variable energetic demand of protein synthesis.

Tissue-specific changes in protein synthesis rates in vivo during anoxia in crucian carp

1996 ◽  
Vol 271 (4) ◽  
pp. R897-R904 ◽  
Author(s):  
R. W. Smith ◽  
D. F. Houlihan ◽  
G. E. Nilsson ◽  
J. G. Brechin
Keyword(s):  
Protein Synthesis ◽  
White Muscle ◽  
Crucian Carp ◽  
Translational Efficiency ◽  
Neural Function ◽  
Tissue Specific ◽  
Significant Mechanism ◽  
Brain Protein Synthesis ◽  
The Brain

Mechanisms of anoxia tolerance were investigated in crucian carp. Rates of protein synthesis were calculated in selected tissues of normoxic and anoxic animals. Exposure to 48 h of anoxia resulted in a significant reduction in protein synthesis in the liver (> 95%), heart (53%), and red and white muscle (52 and 56%, respectively), whereas brain protein synthesis rates were unaffected. Seven days of anoxia produced similar results. After 24 h of recovery from a 48-h anoxic period, protein synthesis rates had virtually returned to normoxic values. The effect of anoxia on the amount of RNA (relative to protein) varied depending on the tissue and also the length of exposure (except in the brain, where it was consistently reduced). However, the effect on RNA translational efficiency was purely tissue specific (i.e., independent of exposure time) and was unaffected in the heart, reduced in the liver and red and white muscle, and increased in the brain. Downregulation of protein synthesis on a tissue-specific basis appears to be a significant mechanism for energy conservation as well as maintaining neural function, thus promoting survival during anoxia.

Relationship between glutamine concentration and protein synthesis in rat skeletal muscle

1988 ◽  
Vol 255 (2) ◽  
pp. E166-E172 ◽  
Author(s):  
M. M. Jepson ◽  
P. C. Bates ◽  
P. Broadbent ◽  
J. M. Pell ◽  
D. J. Millward
Keyword(s):  
Protein Synthesis ◽  
Protein Deficiency ◽  
Synthesis Rate ◽  
Protein Synthesis Rate ◽  
Glutamine Concentration ◽  
E Coli ◽  
Protein Group ◽  
Highly Correlated ◽  
Rna Concentration

Muscle glutamine concentration ([GLN]) and protein synthesis rate (Ks) have been examined in vivo in well-fed, protein-deficient, starved, and endotoxemic rats. With protein deficiency (8 or 5% casein diet), [GLN] fell from 7.70 to 5.58 and 3.56 mmol/kg in the 8 and 5% diet groups, with Ks falling from 15.42 to 9.1 and 6.84%/day. Three-day starvation reduced [GLN] and Ks to 2.38 mmol/kg and 5.6%/day, respectively. In all these groups food intakes and insulin were generally well maintained (except in the starved group), whereas free 3,5,3'-triiodothyronine (T3) was depressed in the starved and 5% protein group. The E. coli lipopolysaccharide endotoxin (3 mg/kg) reduced [GLN] to 5.85 and 4.72 mmol/kg and Ks to 10.5 and 9.10%/day in two well-fed groups. Insulin levels were increased, and free T3 levels fell. Combined protein deficiency and endotoxemia further reduced [GLN] and Ks to 1.88 mmol/kg and 4.01%/day, respectively, in the 5% protein rats. Changes in both ribosomal activity (KRNA) and concentration (RNA/protein) contributed to the fall in Ks in malnutrition and endotoxemia, although reductions in the RNA concentration were most marked with protein deficiency and reductions in the KRNA dominated the response to the endotoxin. The changes in [GLN] and Ks were highly correlated as were [GLN] and both KRNA and the RNA concentration, and these relationships were unique to glutamine. These relationships could reflect sensitivity of glutamine transport and protein synthesis to the same regulatory influences, and the particular roles of insulin and T3 are discussed, as well as any direct influence of glutamine on protein synthesis.

scholarly journals The effect of protein depletion and repletion on muscle-protein turnover in the chick

1981 ◽  
Vol 194 (3) ◽  
pp. 811-819 ◽  
Author(s):  
M L MacDonald ◽  
R W Swick
Keyword(s):  
Protein Synthesis ◽  
Protein Turnover ◽  
Muscle Protein ◽  
Control Diet ◽  
Breast Muscle ◽  
Synthesis Rate ◽  
Protein Depletion ◽  
Protein Mass ◽  
Fractional Rate

Rates of growth and protein turnover in the breast muscle of young chicks were measured in order to assess the roles of protein synthesis and degradation in the regulation of muscle mass. Rates of protein synthesis were measured in vivo by injecting a massive dose of L-[1-14C]valine, and rates of protein degradation were estimated as the difference between the synthesis rate and the growth rate of muscle protein. In chicks fed on a control diet for up to 7 weeks of age, the fractional rate of synthesis decreased from 1 to 2 weeks of age and then changed insignificantly from 2 to 7 weeks of age, whereas DNA activity was constant for 1 to 7 weeks. When 4-week-old chicks were fed on a protein-free diet for 17 days, the total amount of breast-muscle protein synthesized and degraded per day and the amount of protein synthesized per unit of DNA decreased. Protein was lost owing to a greater decrease in the rate of protein synthesis, as a result of the loss of RNA and a lowered RNA activity. When depleted chicks were re-fed the control diet, rapid growth was achieved by a doubling of the fractional synthesis rate by 2 days. Initially, this was a result of increased RNA activity; by 5 days, the RNA/DNA ratio also increased. There was no evidence of a decrease in the fractional degradation rate during re-feeding. These results indicate that dietary-protein depletion and repletion cause changes in breast-muscle protein mass primarily through changes in the rate of protein synthesis.

Regulation of hepatic protein synthesis in chronic inflammation and sepsis

1992 ◽  
Vol 262 (2) ◽  
pp. C445-C452 ◽  
Author(s):  
T. C. Vary ◽  
S. R. Kimball
Keyword(s):  
Protein Synthesis ◽  
Sterile Inflammation ◽  
Chain Elongation ◽  
Synthesis Rate ◽  
Protein Synthesis Rate ◽  
Translational Efficiency ◽  
Liver Protein ◽  
Ribosomal Subunits ◽  
Hepatic Protein Synthesis

The regulation of protein synthesis was determined in livers from control, sterile inflammatory, and septic animals. Total liver protein was increased in both sterile inflammation and sepsis. The rate of protein synthesis in vivo was measured by the incorporation of [3H]phenylalanine into liver proteins in a chronic (5 day) intra-abdominal abscess model. Both sterile inflammation and sepsis increased total hepatic protein synthesis approximately twofold. Perfused liver studies demonstrated that the increased protein synthesis rate in vivo resulted from a stimulation in the synthesis of both secreted and nonsecreted proteins. The total hepatic RNA content was increased 40% only in sterile inflammation, whereas the translational efficiency was increased twofold only in sepsis. The increase in translational efficiency was accompanied by decreases in the amount of free 40S and 60S ribosomal subunits in sepsis. Rates of peptide-chain elongation in vivo were increased 40% in both sterile inflammation and sepsis. These results demonstrate that sepsis induces changes in the regulation of hepatic protein synthesis that are independent of the general inflammatory response. In sterile inflammation, the increase in protein synthesis occurs by a combination of increased capacity and translational efficiency, while in sepsis, the mechanism responsible for accelerated protein synthesis is an increased translational efficiency.

Lymphocyte protein synthesis is increased with the progression of HIV-associated disease to AIDS

2001 ◽  
Vol 101 (6) ◽  
pp. 583-589 ◽  
Author(s):  
Giuseppe CASO ◽  
Peter J. GARLICK ◽  
Marie C. GELATO ◽  
Margaret A. MCNURLAN
Keyword(s):  
Protein Synthesis ◽  
Elevated Serum ◽  
Synthesis Rate ◽  
Hiv Positive ◽  
Lymphocyte Function ◽  
Healthy Controls ◽  
Aids Patients ◽  
Tnf Α

HIV infection has been shown to affect lymphocyte function and to reduce lymphocyte responsiveness in vitro to mitogenic stimulation, but little is known about lymphocyte metabolism in vivo and how it is affected during the course of the disease. This study investigated the metabolic activity of lymphocytes in vivo through the progression of HIV-associated disease. Lymphocyte protein synthesis was measured with l-[2H5]phenylalanine (45mg/kg body weight) in healthy volunteers (n = 7), in patients who were HIV-positive (n = 7) but asymptomatic, and in patients with AIDS (n = 8). The rates of lymphocyte protein synthesis [expressed as a percentage of lymphocyte protein, i.e. fractional synthesis rate (FSR)] were not altered in HIV-positive patients compared with healthy controls (7.9±1.28% and 9.1±0.53%/day respectively), but were significantly elevated in AIDS patients (14.0±1.16%/day; P < 0.05). The serum concentration of the cytokine tumour necrosis factor-α (TNF-α) increased with the progression of the disease, and TNF-α levels were significantly higher in AIDS patients (6.81±0.88ng/l) than in healthy controls (3.09±0.27ng/l; P < 0.05). Lymphocyte protein FSR was positively correlated with serum TNF-α concentration (r = 0.55, P = 0.009) and negatively correlated with CD4+ lymphocyte count (r =-0.70, P = 0.004). The elevation of lymphocyte protein synthesis in AIDS patients suggests a higher rate of turnover of lymphocytes. This may be associated with a generalized activation of the immune system, which is also reflected by the elevated serum TNF-α concentration in the late stages of HIV-associated disease.

scholarly journals In vivo measurement of synthesis rate of multiple plasma proteins in humans

2006 ◽  
Vol 291 (1) ◽  
pp. E190-E197 ◽  
Author(s):  
Abdul Jaleel ◽  
Vandana Nehra ◽  
Xuan-Mai T. Persson ◽  
Yves Boirie ◽  
Maureen Bigelow ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Synthesis ◽  
Stable Isotope ◽  
Plasma Proteins ◽  
Large Scale ◽  
Protein Quantification ◽  
Gas Chromatography Mass Spectrometry ◽  
Synthesis Rate ◽  
Wide Range

Advances in quantitative proteomics have facilitated the measurement of large-scale protein quantification, which represents net changes in protein synthesis and breakdown. However, measuring the rate of protein synthesis is the only way to determine the translational rate of gene transcripts. Here, we report a technique to measure the rate of incorporation of amino acids from ingested protein labeled with stable isotope into individual plasma proteins. This approach involves three steps: 1) production of stable isotope-labeled milk whey protein, oral administration of this intrinsically labeled protein, and subsequent collection of blood samples; 2) fractionation of the plasma and separation of the individual plasma proteins by a combination of anion exchange high-pressure liquid chromatography and gel electrophoresis; and 3) identification of individual plasma proteins by tandem mass spectrometry and measurement of stable isotopic enrichment of these proteins by gas chromatography-mass spectrometry. This method allowed the measurement of the fractional synthesis rate (FSR) of 29 different plasma proteins by using the same precursor pool. We noted a 30-fold difference in FSR of different plasma proteins with a wide range of physiological functions. This approach offers a tremendous opportunity to study the regulation of plasma proteins in humans in many physiological and pathological states.

Tissue-specific changes in protein synthesis associated with seasonal metabolic depression and recovery in the north temperate labrid, Tautogolabrus adspersus

2007 ◽  
Vol 293 (1) ◽  
pp. R474-R481 ◽  
Author(s):  
Johanne M. Lewis ◽  
William R. Driedzic
Keyword(s):  
Protein Synthesis ◽  
Water Temperature ◽  
White Muscle ◽  
Recovery Period ◽  
Synthesis Rate ◽  
Protein Synthesis Rate ◽  
Metabolic Depression ◽  
Tissue Specific ◽  
Total Rna ◽  
Rna Content

The tissue-specific changes in protein synthesis were tracked in relation to the seasonal metabolic depression in cunner ( Tautogolabrus adsperus). In vivo protein synthesis rate and total RNA content were determined in liver, white muscle, brain, heart, and gill during periods of normal activity before metabolic depression, entrance into and during winter dormancy, and during the recovery period. The decrease in water temperature from 8°C to 4°C was accompanied by a 55% depression of protein synthesis in liver, brain, and heart and a 66% depression in gill. Protein synthesis in white muscle fell below detectable levels at this temperature. The depression of protein synthesis is an active process (Q10 = 6–21 between 8°C and 4°C) that occurs in advance of the behavioral and physiological depression at the whole animal level. Protein synthesis was maintained at these depressed levels in white muscle, brain, heart, and gill until water temperature returned to 4°C in the spring. Liver underwent a hyperactivation in the synthesis of proteins at 0°C, which may be linked to antifreeze production. During the recovery period, a hyperactivation of protein synthesis occurred in white muscle, which is suggestive of compensatory growth, as well as in heart and liver, which is considered to be linked to increased activity and feeding. Seasonal changes in total RNA content demonstrate the depression of protein synthesis with decreasing temperature to be closely associated with translational capacity, but the stimulation of protein synthesis during recovery appears to be associated with increased translational efficiency.

Elevated plasma lactate levels via exogenous lactate infusion do not alter resistance exercise-induced signaling or protein synthesis in human skeletal muscle

2020 ◽  
Vol 319 (4) ◽  
pp. E792-E804
Author(s):  
Rasmus Liegnell ◽  
William Apró ◽  
Sebastian Danielsson ◽  
Björn Ekblom ◽  
Gerrit van Hall ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Resistance Exercise ◽  
Blood Lactate ◽  
Intracellular Signaling ◽  
Recovery Period ◽  
Synthesis Rate ◽  
Protein Synthesis Rate ◽  
Lactate Levels ◽  
The Impact

Lactate has been implicated as a potential signaling molecule. In myotubes, lactate incubation increases mechanistic target of rapamycin complex 1 (mTORC1)- and ERK-signaling and induces hypertrophy, indicating that lactate could be a mediator of muscle adaptations to resistance exercise. However, the potential signaling properties of lactate, at rest or with exercise, have not been explored in human tissue. In a crossover design study, 8 men and 8 women performed one-legged resistance exercise while receiving venous infusion of saline or sodium lactate. Blood was sampled repeatedly, and muscle biopsies were collected at rest and at 0, 90, and 180 min and 24 h after exercise. The primary outcomes examined were intracellular signaling, fractional protein synthesis rate (FSR), and blood/muscle levels of lactate and pH. Postexercise blood lactate concentrations were 130% higher in the Lactate trial (3.0 vs. 7.0 mmol/L, P < 0.001), whereas muscle levels were only marginally higher (27 vs. 32 mmol/kg dry wt, P = 0.003) compared with the Saline trial. Postexercise blood pH was higher in the Lactate trial (7.34 vs. 7.44, P < 0.001), with no differences in intramuscular pH. Exercise increased the phosphorylation of mTORS2448 (∼40%), S6K1T389 (∼3-fold), and p44T202/T204 (∼80%) during recovery, without any differences between trials. FSR over the 24-h recovery period did not differ between the Saline (0.067%/h) and Lactate (0.062%/h) trials. This study does not support the hypothesis that blood lactate levels can modulate anabolic signaling in contracted human muscle. Further in vivo research investigating the impact of exercised versus rested muscle and the role of intramuscular lactate is needed to elucidate its potential signaling properties.

Glucocorticoid-induced skeletal muscle atrophy in vitro is attenuated by mechanical stimulation

1992 ◽  
Vol 262 (6) ◽  
pp. C1471-C1477 ◽  
Author(s):  
J. A. Chromiak ◽  
H. H. Vandenburgh
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Protein Content ◽  
Total Protein ◽  
Weight Lifting ◽  
Mechanical Activity ◽  
Synthesis Rate ◽  
Protein Synthesis Rate

Glucocorticoids induce rapid atrophy of fast skeletal myofibers in vivo, and either weight lifting or endurance exercise reduces this atrophy by unknown mechanisms. We examined the effects of the synthetic glucocorticoid dexamethasone (Dex) on protein turnover in tissue-cultured avian fast skeletal myofibers and determined whether repetitive mechanical stretch altered the myofiber response to Dex. In static cultures after 3-5 days, 10(-8) M Dex decreased total protein content 42-74%, total protein synthesis rates 38-56%, mean myofiber diameter 35%, myosin heavy chain (MHC) content 86%, MHC synthesis rate 44%, and fibronectin synthesis rate 29%. Repetitive 10% stretch-relaxations of the cultured myofibers for 60 s every 5 min for 3-4 days prevented 52% of the Dex-induced decrease in protein content, 42% of the decrease in total protein synthesis rate, 77% of the decrease in MHC content, 42% of the decrease in MHC synthesis rate, and 67% of the decrease in fibronectin synthesis rate. This in vitro model system will complement in vivo studies in understanding the mechanism by which mechanical activity and glucocorticoids interact to regulate skeletal muscle growth.

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