Turnover of fibrinogen, plasminogen, and prothrombin during exercise in man

1977 ◽  
Vol 42 (6) ◽  
pp. 865-873 ◽  
Author(s):  
D. Collen ◽  
N. Semeraro ◽  
J. P. Tricot ◽  
J. Vermylen

The turnover of biologically intact high-purity 125I- and 131I-labeled fibrinogen, plasminogen, and/or prothrombin was studied in 16 untrained healthy subjects before and during strenuous physical exercise on a bicycle ergometer (repeated 4 times/day for 2 days). The exertion resulted in the well-known changes in the coagulation and fibrinolytic tests but did not induce changes in the concentration of fibrinogen, plasminogen, or prothrombin in the plasma. A significantly increased catabolism of fibrinogen and plasminogen but not of prothrombin was observed. The extent of Aalpha-chain degradation of fibrinogen in the plasma was quantitated before and 2 h after exercise, and a significant increase in degraded Aalpha chains was found in the postexercise samples. Immunochemical estimation of plasmin-antiplasmin complex in four subjects revealed a small increase after exercise in three of them. All these data support the concept that plasminogen activation and plasmin-induced fibrinogen degradation occur to some extent in man following strenuous physical exercise.

1982 ◽  
Vol 53 (1) ◽  
pp. 40-43 ◽  
Author(s):  
P. Bartsch ◽  
E. K. Schmidt ◽  
P. W. Straub

To examine hemostasis after physical exercise at altitudes easily accessible to tourists by public transport, 20 young male volunteers were exposed to 3,457 m above sea level. Ten of them were subjected to an exhaustive exercise for about 8 min on a bicycle ergometer. The preexercise samples (n = 20) taken 1 h after arrival showed no significant alteration of coagulation compared with control values at 600 m. After the exercise the clotting times (P less than 0.001) and euglobulin lysis times (P less than 0.001) were shortened, whereas factor VIII activity (P less than 0.001) was elevated. There was, however, no significant difference in fibrinopeptide A levels between the exercise and the control group. Ethanol gelation test remained negative. We found no rise in fibrin(ogen) degradation products and fibrin(ogen) fragment E and thus conclude that there is no evidence for clinically relevant intravascular coagulation after short-term strenuous physical exercise at altitude.


1991 ◽  
Vol 65 (01) ◽  
pp. 082-086 ◽  
Author(s):  
G Dooijewaard ◽  
A de Boer ◽  
P N C Turion ◽  
A F Cohen ◽  
D D Breimer ◽  
...  

SummaryThe enhancement of the blood fibrinolytic potential by physical exercise is generally attributed to the release of tissue-type plasminogen activator (t-PA) from the vessel wall. In this study we have investigated the possible contribution of urokinase-type plasminogen activator (u-PA).Six healthy male volunteers (age 21–25 years) were screened for their ability to perform maximal exercise for their age-group for 12 min on a bicycle ergometer. Subsequently, on one occasion they were required to remain supine for 2 h (from 8.30 a. m. onwards) and on another they performed maximal exercise (from 9.00 a.m. onwards). During exercise an increase in u-PA antigen and plasmin-activatable pro-urokinase (proUK) activity, concurrent with t-PA antigen and euglobulin t-PA activity, was observed in all six volunteers, while at rest these parameters remained unaffected. Mean u-PA- and t-PA antigen increased, respectively, from 4.2 ± 1.0 ng/ml and 5.8 ± 2.1 ng/ml before exercise to 9.8 ± 3.0 ng/ml and 18.3 ± 3.8 ng/ml (peak). Mean plasminactivatable proUK activity and t-PA activity increased, respectively, from 2.1 ± 0.4 ng/ml and 0.3 ± 0.2 ng/ml before exercise to 4.3 ± 1.7 ng/ml and 7.2 ± 4.0 ng/ml (peak). The increases were statistically significant throughout (paired t-test, pre vs post, antigen P <0.005 and activity P <0.02). After cessation of exercise u-PA and t-PA declined concurrently to normal values with a 50"/" decay in about 5 min. In conclusion, we found that both u-PA antigen and plasmin-activatable proUK activity are, concurrently with t-PA, enhanced upon exercise and, therefore, we consider that u-PA also contributes to – and co-operates in – the enhancement of the blood fibrinolytic potential and activity under these conditions.


2020 ◽  
Vol 21 (Supplement_1) ◽  
Author(s):  
K Wdowiak-Okrojek ◽  
P Wejner-Mik ◽  
Z Bednarkiewicz ◽  
P Lipiec ◽  
J D Kasprzak

Abstract Background Stress echocardiography (SE) plays an important role among methods of noninvasive diagnosis of ischemic disease. Despite the advantages of physical exercise as the most physiologic stressor, it is difficult (bicycle ergometer) or impossible (treadmill) to obtain and maintain the acoustic window during the exercise. Recently, an innovative probe fixation device was introduced and a research plan was developed to assess the feasibility of external probe fixation during exercise echocardiography on a supine bicycle and upright treadmill exercise for the first time. Methods 37 subjects (36 men, mean age 39 ± 16 years, 21 healthy volunteers, 16 patients with suspected coronary artery disease) were included in this study. This preliminary testing stage included mostly men due to more problematic probe fixation in women. All subjects underwent a submaximal exercise stress test on a treadmill (17/37) or bicycle ergometer (11/37). Both sector and matrix probes were used. We assessed semi-quantitatively the quality of acquired apical views at each stage – the four-point grading system was used (0-no view, 1-suboptimal quality, 2-optimal quality, 3-very good quality), 2-3 sufficient for diagnosis. Results The mean time required for careful positioning of the probe and image optimization was 12 ± 3 min and shortened from 13,7 to 11,1 minutes (mean) in first vs second half of the cohort documenting learning curve. At baseline, 9 patients had at least one apical view of quality precluding reliable analysis. Those patients were excluded from further assessment. During stress, 17 patients maintained the optimal or very good quality of all apical views, whereas in 11 patients the quality significantly decreased during the stress test and required probe repositioning. The mean image quality score at baseline was 2,61 ± 0,48 and 2,25 ± 0,6 after exercise. Expectedly, good image quality was easier to obtain and maintain in the supine position (score 2,74 ± 0,44) points as compared with upright position (score 2,25 ± 0,57). Conclusion This preliminary, unique experience with external probe fixation device indicates that continuous acquisition and monitoring of echocardiographic images is feasible during physical exercise, and for the first time ever - also on the treadmill. This feasibility data stem from almost exclusively male patients and the estimated rate of sufficient image quality throughout the entire test is currently around 60%. We are hoping, that gaining more experience with the product could increase the success rate on exercise tests. Abstract P1398 Figure. Treadmill and ergometer stress test


Author(s):  
Simona Daniele ◽  
Deborah Pietrobono ◽  
Jonathan Fusi ◽  
Annalisa Lo Gerfo ◽  
Eugenio Cerri ◽  
...  

2009 ◽  
Vol 2 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Vaibhav Moondra ◽  
Satyam Sarma ◽  
Tracy Buxton ◽  
Radwan Safa ◽  
Gregory Cote ◽  
...  

Purpose: Neuregulins (NRG) are growth factors that bind to receptors of the erbB family, and are known to mediate a number of processes involved in diverse tissues. Neuregulin-1β is expressed in skeletal muscle and is activated by exercise. We hypothesized that NRG-1β might circulate in the bloodstream and increase as a consequence of physical activity. A study was conducted in healthy subjects to determine if NRG-1β is immunodetectable in human serum, and if so whether levels relate acutely or chronically to exercise. Methods: Nine healthy men underwent three bouts of exercise of varying degrees of intensity on a bicycle ergometer over a period of three weeks. Cardio-respiratory fitness was determined by measurement of maximal oxygen uptake (VO2max). Serum was sampled prior to and immediately after each session (up to 30 minutes post) and serum NRG-1ß was quantified utilizing an indirect sandwich ELISA assay developed in our lab. Results: Across subjects, mean serum NRG-1β levels ranged from 32 ng/mL to 473 ng/mL. Individual subjects showed relatively stable levels during the study period that did not change acutely after exercise. Serum NRG-1β demonstrated a positive correlation with VO2max (r2=0.49, p =.044). Conclusions: These preliminary observations suggest that at least in healthy men, serum NRG-1β is an indicator of cardio- respiratory fitness and does not change acutely with exercise.


2010 ◽  
Vol 110 (6) ◽  
pp. 1155-1162 ◽  
Author(s):  
W. Dudzinska ◽  
A. Lubkowska ◽  
B. Dolegowska ◽  
K. Safranow ◽  
K. Jakubowska

2011 ◽  
Vol 47 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Vicky Chaar ◽  
Marc Romana ◽  
Julien Tripette ◽  
Cédric Broquere ◽  
Marie-Geneviève Huisse ◽  
...  

1965 ◽  
Vol 20 (1) ◽  
pp. 137-141 ◽  
Author(s):  
Gunnar Grimby

Clearance of inulin (CIn) and para-aminohippuric acid (CPAH), cardiac output, oxygen uptake, and arterial blood pressure were measured in five healthy subjects at rest and during supine exercise on a bicycle ergometer before and after injection of a bacterial pyrogen (purified lipopolysaccharide, Pyrexal). CPAH was 45–145% higher at rest during the flush phase than in the normal condition. The increase in CIn, was less. Cardiac output increased also. The renal fraction of the cardiac output was larger than in the normal condition. During moderate exercise in the flush phase, CPAH decreased from the values before work. This decrease was usually larger than in the normal condition. The pyrogen-induced increase in cardiac output was less during exercise than at rest. A pronounced renal vasoconstriction during exercise is demonstrated even after the injection of bacterial pyrogen. clearance of inulin and para-aminohippuric acid; cardiac output; extraction ratio Submitted on May 4, 1964


2007 ◽  
Vol 20 (03) ◽  
pp. 149-153 ◽  
Author(s):  
X. Lin ◽  
M. El-Sayed ◽  
J. Waterhouse ◽  
T. Reilly

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