Superoxide dismutase derivative reduces oxidative damage in skeletal muscle of rats during exhaustive exercise

1995 ◽  
Vol 79 (1) ◽  
pp. 129-135 ◽  
Author(s):  
Z. Radak ◽  
K. Asano ◽  
M. Inoue ◽  
T. Kizaki ◽  
S. Oh-Ishi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Superoxide Dismutase ◽  
Xanthine Oxidase ◽  
Thiobarbituric Acid ◽  
Experimental Period ◽  
Treadmill Running ◽  
Exhaustive Exercise ◽  
Thiobarbituric Acid Reactive Substances ◽  
Exercise Induced

A superoxide dismutase derivative (SM-SOD) that circulates and is bound to albumin with a half-life of 6 h was injected intraperitoneally into rats before exhaustive treadmill running to study its antioxidant scavenging capacity in the plasma and soleus and tibialis muscles. The exercise induced a marked increase in xanthine oxidase activity in plasma and an increase in thiobarbituric acid-reactive substances in the plasma as well as in the soleus and tibialis muscles of nonadministered rats immediately after the exercise. The immunoreactive content and activity of both SOD isoenzymes (Cu,Zn-SOD and Mn-SOD) of the nonadministered rats increased in the soleus and tibialis muscles immediately after running. SM-SOD treatment definitely attenuated the degree of the increase in thiobarbituric acid-reactive substances and xanthine oxidase in all samples examined immediately after exercise. Glutathione peroxidase activity significantly increased in the soleus muscle of nonadministered rats 1 day after running, whereas catalase activity remained unchanged throughout the experimental period. These results suggest that a single bout of exhaustive exercise induces oxidative stress in skeletal muscle of rats and that this oxidative stress can be attenuated by exogenous SM-SOD.

Exercise-induced oxidative stress: glutathione supplementation and deficiency

1994 ◽  
Vol 77 (5) ◽  
pp. 2177-2187 ◽  
Author(s):  
C. K. Sen ◽  
M. Atalay ◽  
O. Hanninen
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Critical Role ◽  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Repeated Administration ◽  
Exhaustive Exercise ◽  
Relative Role ◽  
Exercise Induced

Glutathione (GSH) plays a central role in coordinating the synergism between different lipid- and aqueous-phase antioxidants. We documented 1) how exogenous GSH and N-acetylcysteine (NAC) may affect exhaustive exercise-induced changes in tissue GSH status, lipid peroxides [thiobarbituric acid-reactive substances (TBARS)], and endurance and 2) the relative role of endogenous GSH in the circumvention of exercise-induced oxidative stress by using GSH-deficient [L-buthionine-(S,R)-sulfoximine (BSO)-treated] rats. Intraperitoneal injection of GSH remarkably increased plasma GSH; exogenous GSH per se was an ineffective delivery agent of GSH to tissues. Repeated administration of GSH (1 time/day for 3 days) increased blood and kidney total GSH [TGSH; GSH+oxidized GSH (GSSG)]. Neither GSH nor NAC influenced endurance to exhaustion. NAC decreased exercise-induced GSH oxidation in the lung and blood. BSO decreased TGSH pools in the liver, lung, blood, and plasma by approximately 50% and in skeletal muscle and heart by 80–90%. Compared with control, resting GSH-deficient rats had lower GSSG in the liver, red gastrocnemius muscle, heart, and blood; similar GSSG/TGSH ratios in the liver, heart, lung, blood, and plasma; higher GSSG/TGSH ratios in the skeletal muscle; and more TBARS in skeletal muscle, heart, and plasma. In contrast to control, exhaustive exercise of GSH-deficient rats did not decrease TGSH in the liver, muscle, or heart or increase TGSH of plasma; GSSG of muscle, blood, or plasma; or TBARS of plasma or muscle. GSH-deficient rats had approximately 50% reduced endurance, which suggests a critical role of endogenous GSH in the circumvention of exercise-induced oxidative stress and as a determinant of exercise performance.

scholarly journals Effect of Acacia Polyphenol Supplementation on Exercise-Induced Oxidative Stress in Mice Liver and Skeletal Muscle

Antioxidants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 29 ◽  
Author(s):  
Koichi Yada ◽  
Llion Arwyn Roberts ◽  
Natsumi Oginome ◽  
Katsuhiko Suzuki
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Muscle Protein ◽  
Thiobarbituric Acid ◽  
Exhaustive Exercise ◽  
Protein Carbonyls ◽  
High Dose ◽  
Thiobarbituric Acid Reactive Substance ◽  
Skeletal Muscle Protein ◽  
Exercise Induced

The purpose of this study was to investigate the effects of acacia polyphenol (AP) supplementation on exercise-induced oxidative stress in mouse liver and skeletal muscle. Plasma aspartate aminotransferase (AST), liver and skeletal muscle levels of thiobarbituric acid reactive substance (TBARS), and levels of skeletal muscle protein carbonyls increased immediately after exhaustive exercise. Exhaustive exercise also decreased liver glutathione (GSH). These results suggest that the exhaustive exercise used in this study induced tissue damage and oxidative stress. Contrary to our expectations, AP supplementation increased plasma AST and alanine aminotransferase activities, liver levels of TBARS, and protein carbonyls. Furthermore, AP supplementation decreased glutathione and glutathione peroxidase activity in the liver. On the other hand, AP supplementation decreased TBARS levels in skeletal muscle. These results suggest that oral high-dose AP administration decreased oxidative stress in skeletal muscle but induced oxidative stress in the liver and increased hepatotoxicity.

scholarly journals Spirulina maxima and its effect on antioxidant activity in fructose induced oxidative stress with histopathological observations

2015 ◽  
Vol 62 (2) ◽  
pp. 13-19
Author(s):  
Urmila Jarouliya ◽  
Anish Zacharia ◽  
Raj K. Keservani ◽  
Godavarthi B.K.S Prasad
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Glucose ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Therapeutic Effects ◽  
Thiobarbituric Acid Reactive Substances ◽  
Spirulina Maxima

Abstract Diabetes mellitus is a metabolic disorder characterised by hyperglycemia and oxidative stress. The aim of the present study is to explore the antioxidant effect of Spirulina maxima in rat model along with the histopathological observations. Diabetes was induced by feeding 10% fructose solution orally to Wistar rats (n = 6) for 30 days, analysed for plasma blood glucose and the markers of the oxidative stress [catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS)]. These biochemical studies were associated with histopathological examination of liver and kidney sections. The microalga Spirulina maxima being rich in proteins and other essential nutrients is widely used as a food supplement. S. maxima at a dose of 5 and 10% per kg and the metformin (500 mg/kg) as reference drug were given orally for 30 days to the diabetic rats. Diabetic rats showed significant (p < 0.001) elevations in plasma blood glucose, thiobarbituric acid-reactive substances and significant reduction in catalase, superoxide dismutase and reduced glutathione activity. Oral administration of 5 and 10% aqueous extract of S. maxima for 30 days restored not only of blood glucose levels but also markers of oxidative stress. Histopathological observations of tissues manifested that the S. maxima administration had the protective and therapeutic effects against fructose-induced abnormalities in diabetic rats. It is concluded that S. maxima is effective in reinstating the antioxidant activity in addition to its antidiabetic effect in type 2 diabetic rats.

scholarly journals Lycopene supplementation attenuated xanthine oxidase and myeloperoxidase activities in skeletal muscle tissues of rats after exhaustive exercise

2005 ◽  
Vol 94 (4) ◽  
pp. 595-601 ◽  
Author(s):  
Chieh-Chung Liu ◽  
Chi-Chang Huang ◽  
Wan-Teng Lin ◽  
Chin-Cheng Hsieh ◽  
Shih-Yi Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Xanthine Oxidase ◽  
Low Dose ◽  
Exhaustive Exercise ◽  
Strenuous Exercise ◽  
High Dose ◽  
Sedentary Control ◽  
Muscle Tissues ◽  
Significant Difference

Strenuous exercise is known to induce oxidative stress leading to the generation of free radicals. The purpose of the present study was to investigate the effects of lycopene, an antioxidant nutrient, at a relatively low dose (2·6 mg/kg per d) and a relatively high dose (7·8 mg/kg per d) on the antioxidant status of blood and skeletal muscle tissues in rats after exhaustive exercise. Rats were divided into six groups: sedentary control (C); sedentary control with low-dose lycopene (CLL); sedentary control with high-dose lycopene (CHL); exhaustive exercise (E); exhaustive exercise with low-dose lycopene (ELL); exhaustive exercise with high-dose lycopene (EHL). After 30 d, the rats in the three C groups were killed without exercise, but the rats in the three E groups were killed immediately after an exhaustive running test on a motorised treadmill. The results showed that xanthine oxidase (XO) activities of plasma and muscle, and muscular myeloperoxidase (MPO) activity in group E were significantly increased compared with group C. Compared with group E, the elevations of XO and MPO activities of muscle were significantly decreased in group EHL. The malondialdehyde concentrations of plasma and tissues in group E were significantly increased by 72 and 114 %, respectively, compared with those in group C. However, this phenomenon was prevented in rats of the ELL and EHL groups. There was no significant difference in the GSH concentrations of erythrocytes in each group; however, exhaustive exercise resulted in a significant decrease in the GSH content of muscle. In conclusion, these results suggested that lycopene protected muscle tissue from oxidative stress after exhaustive exercise.

scholarly journals Effect of silymarin on sodium fluoride-induced toxicity and oxidative stress in rat cardiac tissues

2012 ◽  
Vol 84 (4) ◽  
pp. 1121-1126 ◽  
Author(s):  
Seyed M. Nabavi ◽  
Seyed F. Nabavi ◽  
Akbar H. Moghaddam ◽  
William N. Setzer ◽  
Morteza Mirzaei
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Sodium Fluoride ◽  
Biochemical Parameters ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Glutathione Level ◽  
Thiobarbituric Acid Reactive Substances ◽  
Cardiac Tissues ◽  
And Oxidative Stress

This study aim to evaluate the protective effect of silymarin on sodium fluoride-induced oxidative stress in rat cardiac tissues. Animals were pretreated with silymarin at 20 and 10 mg/kg prior to sodium fluoride consumption (600 ppm through drinking water). Vitamin C at 10 mg/kg was used as standard antioxidant. There was a significant increase in thiobarbituric acid reactive substances level (59.36 ± 2.19 nmol MDA eq/g tissue) along with a decrease in antioxidant enzymes activity (64.27 ± 1.98 U/g tissue for superoxide dismutase activity and 29.17 ± 1.01 µmol/min/mg protein for catalase activity) and reduced glutathione level (3.8 ± 0.15 µg/mg protein) in the tissues homogenates of the sodium fluoride-intoxicated rats. Silymarin administration to animals before sodium fluoride consumption modified the levels of biochemical parameters.

Antioxidant effect of organic purple grape juice on exhaustive exercise

2013 ◽  
Vol 38 (5) ◽  
pp. 558-565 ◽  
Author(s):  
Cristiane L. Dalla Corte ◽  
Nélson R. de Carvalho ◽  
Guilherme P. Amaral ◽  
Gustavo O. Puntel ◽  
Luiz Fernando A. Silva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Grape Juice ◽  
Exercise Bout ◽  
Thiobarbituric Acid ◽  
Exhaustive Exercise ◽  
Rat Tissues ◽  
Dichlorofluorescein Diacetate ◽  
Rat Tissue ◽  
The Brain

This study aimed to assess the potential protective effect of organic purple grape juice (PGJ) on oxidative stress produced by an exhaustive exercise bout in rats. To test this hypothesis, rats were acutely treated with organic PGJ (Vitis labrusca) and subsequently submitted to an exhaustive exercise bout. Parameters of oxidative stress, such as thiobarbituric acid reactive species (TBARS) levels, 2′,7′,-dichlorofluorescein diacetate (DCFH-DA) oxidation, and nonprotein sulfhydryl levels (NP-SH) in the brain, skeletal muscle, and blood, were evaluated. Enzyme activity of Na+,K+-ATPase, Ca2+-ATPase, and δ-aminolevulinate dehydratase (δ-ALA-D) in the brain, skeletal muscle, and blood were also assayed. Statistical analysis showed that the exhaustive exercise bout increased TBARS levels and DCFH-DA oxidation, and decreased NP-SH levels in rat tissue. Ca2+-ATPase activity was increased in groups exposed to both exercise and PGJ treatment. The results indicate that organic PGJ intake was able to protect against the oxidative damage caused by an exhaustive exercise bout in different rat tissues.

scholarly journals The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 866
Author(s):  
Takaki Tominaga ◽  
Tsukasa Ikemura ◽  
Koichi Yada ◽  
Kazue Kanda ◽  
Kaoru Sugama ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Organ Damage ◽  
Exhaustive Exercise ◽  
Strenuous Exercise ◽  
Intestinal Damage ◽  
Urine Biomarkers ◽  
Exercise Induced ◽  
And Oxidative Stress ◽  
Healthy Males

Strenuous exercise induces organ damage, inflammation and oxidative stress. To prevent exercise-induced organ damage, inflammation and oxidative stress, rehydrating may be an effective strategy. In the present study, we aimed to examine whether beverage intake after exhaustive exercise to recover from dehydration prevents such disorders. Thirteen male volunteers performed incremental cycling exercise until exhaustion. Immediately after exercise, the subjects drank an electrolyte containing water (rehydrate trial: REH) or did not drink any beverage (control trial: CON). Blood samples were collected before (Pre), immediately (Post), 1 h and 2 h after exercise. Urine samples were also collected before (Pre) and 2 h after exercise. We measured biomarkers of organ damage, inflammation and oxidative stress in blood and urine. Biomarkers of muscle, renal and intestinal damage and inflammation increased in the blood and urine after exercise. However, changes in biomarkers of organ damage and inflammation did not differ between trials (p > 0.05). The biomarker of oxidative stress, thiobarbituric acid reactive substances (TBARS), in plasma, showed different changes between trials (p = 0.027). One hour after exercise, plasma TBARS concentration in REH had a higher trend than that in CON (p = 0.052), but there were no significant differences between Pre and the other time points in each trial. These results suggest that beverage intake after exercise does not attenuate exercise-induced organ damage, inflammation or oxidative stress in healthy males. However, rehydration restores exercise-induced oxidative stress more quickly.

scholarly journals Ginsenoside-Rg1 Protects the Liver against Exhaustive Exercise-Induced Oxidative Stress in Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Mallikarjuna Korivi ◽  
Chien-Wen Hou ◽  
Chih-Yang Huang ◽  
Shin-Da Lee ◽  
Ming-Fen Hsu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Thiobarbituric Acid ◽  
Exhaustive Exercise ◽  
Protein Carbonyls ◽  
Regular Exercise ◽  
Thiobarbituric Acid Reactive Substance ◽  
Ginsenoside Rg1 ◽  
Exercise Induced ◽  
First Time

Despite regular exercise benefits, acute exhaustive exercise elicits oxidative damage in liver. The present study determined the hepatoprotective properties of ginsenoside-Rg1 against exhaustive exercise-induced oxidative stress in rats. Forty rats were assigned into vehicle and ginsenoside-Rg1 groups (0.1 mg/kg bodyweight). After 10-week treatment, ten rats from each group performed exhaustive swimming. Estimated oxidative damage markers, including thiobarbituric acid reactive substance (TBARS) (67%) and protein carbonyls (56%), were significantly (P<0.01) elevated after exhaustive exercise but alleviated in ginsenoside-Rg1 pretreated rats. Furthermore, exhaustive exercise drastically decreased glutathione (GSH) content (∼79%) with concurrent decreased superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities. However, these changes were attenuated in Rg1 group. Additionally, increased xanthine oxidase (XO) activity and nitric oxide (NO) levels after exercise were also inhibited by Rg1 pretreatment. For the first time, our findings provide strong evidence that ginsenoside-Rg1 can protect the liver against exhaustive exercise-induced oxidative damage.

scholarly journals The effects of superoxide dismutase knockout on the oxidative stress parameters and survival of mouse erythrocyt

2009 ◽  
Vol 14 (1) ◽  
Author(s):  
Agnieszka Grzelak ◽  
Marcin Kruszewski ◽  
Ewa Macierzyńska ◽  
Łukasz Piotrowski ◽  
Łukasz Pułaski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Blood Plasma ◽  
Reticulocyte Count ◽  
Thiobarbituric Acid ◽  
Protective Effects ◽  
Thiobarbituric Acid Reactive Substances ◽  
Heinz Bodies ◽  
Plasma Glutathione ◽  
Activity Decrease

AbstractThe erythrocytes of 12-month old Sod1 −/− mice showed an increased level of reactive oxygen species (ROS), as estimated by the degree of dihydroethidine and dihydrorhodamine oxidation, and the increased level of Heinz bodies. No indices of severe oxidative stress were found in the red blood cells and blood plasma of Sod1 −/− mice as judged from the lack of significant changes in the levels of erythrocyte and plasma glutathione, plasma protein thiol and carbonyl groups and thiobarbituric-acid reactive substances in the blood plasma. However, a decreased erythrocyte lifespan, increased reticulocyte count and splenomegaly were noted, indicating the importance of superoxide dismutase for maintaining erythrocyte viability. The levels of erythrocyte ROS and Heinz bodies and the reticulocyte count were indistinguishable in Sod1 +/+ and Sod1 +/− mice, suggesting that a superoxide dismutase activity decrease to half of its normal value may be sufficient to secure the protective effects of the enzyme.

scholarly journals Silicone dioxide nano-particles enhance toxicity of lead on oxidative and nitro-oxidative stress

2017 ◽  
Author(s):  
I. A. Bandas ◽  
M. I. Kulitska ◽  
T. Ya. Yaroshenko ◽  
M. M. Korda
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Silicon Dioxide ◽  
Blood Serum ◽  
Lead Acetate ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Silicon Dioxide Nanoparticles ◽  
Modified Proteins

Introduction. Nanoparticles are widely used in scientific research, industry and medicine. The established capability of nanoparticles to increase the transport of chemicals and drugs into cells and through the body barriers makes the possibility of potentiating the chemical contaminants toxicity in case of their simultaneous intake an urgent matter.The aim of the study – to learn the effect of silicon dioxide nanoparticles on the ability of chemical lead acetate toxicant to cause oxidative and nitro-oxidative stress in blood serum and liver of experimental rats.Research Methods. Experiments were conducted on 40 white outbred male rats, 150–160 g in weight, which were divided into 4 groups. Animals of the group (control) 1 were daily administered with saline solution intragastrically. The rats of the group 2 were administered with colloidal solution of silicon dioxide nanoparticles in a dose of 50 mg/kg of body weight. Animals of the group 3 were injected with lead acetate in aqueous solution in a dose of 20 mg/kg of body weight (expressed as lead), the group 4 – with solution of silicon dioxide nanoparticles with lead acetate daily during 3 weeks at the same doses. The total activity of NO-synthase, catalase, superoxide dismutase, NOx content, thiobarbituric acid reactive substances, oxidized modified proteins, reduced glutathione, ceruloplasmin and total serum antioxidant activity were determined in serum and liver. The obtained parameters were statistically processed.Results and Discussion. It was proved that silicon dioxide nanoparticles did not influence the studied parameters considerably. The administration of lead acetate to rats caused significant changes of all indices. However, the maximum changes of the parameters were evidenced in the group of animals in cases of simultaneous administration of silicon dioxide nanoparticles and lead acetate. In that case, the content of thiobarbituric acid reactive substances, NOx, oxidized modified proteins, reduced glutathione, and superoxide dismutase activity in blood serum and liver homogenate of rats varied significantly compared with the parameters of the group of animals that were administered with the chemical toxicant only.Conclusion. Silicon dioxide nanoparticles enhance the capability of the chemical lead acetate toxicant to cause oxidative and nitro-oxidative stress in blood serum and liver of the experimental rats.

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