scholarly journals UV Spectrophotometric Method for Determination of Cinitapride in Pure and its Solid Dosage Form

2009 ◽  
Vol 6 (s1) ◽  
pp. S21-S24 ◽  
Author(s):  
B. Thangabalan ◽  
A. Elphine Prabahar ◽  
R. Kalaichelvi ◽  
P. Vijayaraj Kumar
Keyword(s):  
Quality Control ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Analytical Method ◽  
Dosage Form ◽  
Spectrophotometric Assay ◽  
Solid Dosage ◽  
Quality Control Testing ◽  
Routine Quality Control

A new, rapid, precise, accurate and sensitive analytical method was developed for the UV spectrophotometric assay of cinitapride (CTP). The drug obeyed the Beer's law and showed good correlation. It showed absorption maxima at 260 nm in methanol. The linearity was observed between 5-40 µg mL-1. The results of analysis were validated by recovery studies. The recovery was more than 99%. The proposed method is the only method available for spectrophotometric determination of the drug. It is simple, precise, sensitive and reproducible and can be used for the routine quality control testing of the marketed formulations.

UV spectroscopic method for determination of phenytoin in bulk and injection forms

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Spectroscopic Method ◽  
Ich Guidelines ◽  
Precision And Accuracy ◽  
Routine Quality Control

Recent study was conducted to develop a simple UV spectrophotometric method to determine Phenytoin in bulk and injection form according to official requirement and validate as per ICH guidelines. λmax of Phenytoin was found 202 nm. Linearity existed perceived in the concentration assortment 2-8 μg/ml (r2 = 0.999) for the method. The method was validated pertaining to linearity, precision and accuracy studies, LOD and LOQ consistent with ICH guidelines. The existent method was establish to be simple, linear, precise, accurate as well as sensitive and can be applied for routine quality control enquiry for the analysis of Phenytoin in bulk and injection form.

scholarly journals Comparison of the Robustness of Spectrophotometric Method and High-Performance Liquid Chromatographic Method for the Determination of Pioglitazone in Solid Dosage Form

2021 ◽  
pp. 37-45
Author(s):  
Sara Zahid ◽  
Fatima Zahid ◽  
Asma Ahmed ◽  
Waqas Safir
Keyword(s):  
Spectrophotometric Method ◽  
Analytical Method ◽  
High Performance ◽  
Dosage Form ◽  
Raw Materials ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
The Body ◽  
Solid Dosage Form ◽  
Solid Dosage

Pioglitazone is a drug that reduces the amount of glucose (sugar) in the blood. It is included in the class of anti-diabetic drugs called “thiazolidinedione” that are used in the treatment of type II diabetes. It attaches to the peroxisomes proliferated- activated receptor gamma (PPARϒ) on tissues throughout the body and causes the cells to become more sensitive to insulin. As a result, more glucose is removed from the blood.The aim of the study is more precisely to find out the better analytical method for the quantitative measurement of the content of pioglitazone in commercially available drugs using two analytical methods i-e Spectrophotometric method and HPLC method.The analytical method for the pioglitazone hydrochloride was developed by HPLC, and then validated the method according to compendial requirements. Pioglitazone in various Dowglit and Gliden tablets was determined by this developed methodPrecision, Accuracy and stability of Pioglitazone was checked which came out to be100.40% and 100.19% respectively. The analysis of pioglitazone hydrochloride in solid dosage form using the HPLC method shows the results are more sensitive, accurate, validated and economical and can be easily applied to raw materials and finished goods compared to the Spectrophotometer method.

scholarly journals Development and validation of UV spectrophotometric method for orbifloxacin assay and dissolution studies

2014 ◽  
Vol 50 (3) ◽  
pp. 457-465 ◽  
Author(s):  
Edith Cristina Laignier Cazedey ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Quality Control ◽  
Hydrochloric Acid ◽  
Spectrophotometric Method ◽  
Cost Effective ◽  
Pharmaceutical Formulation ◽  
Dissolution Studies ◽  
Linearity Range ◽  
Development And Validation ◽  
Routine Quality Control

New, simple and cost effective UV-spectrophotometric method was developed for the estimation of orbifloxacin in pharmaceutical formulation. Orbifloxacin was estimated at 290 nm in 0.5 M hydrochloric acid. Linearity range was found to be 1.0-6.0 μg mL-1. The method was tested and validated for various parameters according to main guidelines. The proposed method was successfully applied for the determination of orbifloxacin in tablets. The results demonstrated that the procedure is accurate, precise and reproducible, while being simple, economical and less time consuming. It can be suitably applied for the estimation of orbifloxacin in routine quality control and dissolution studies.

scholarly journals Simultaneous Spectrophotometric Method for Determination of Emtricitabine and Tenofovir Disoproxil Fumarate in Three-Component Tablet Formulation Containing Rilpivirine Hydrochloride

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
S. Venkatesan ◽  
N. Kannappan
Keyword(s):  
Spectrophotometric Method ◽  
Analytical Method ◽  
Tenofovir Disoproxil Fumarate ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Tablet Dosage

Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A complexation, derivatization, extraction, evaporation, and sensitive-free direct UV spectrophotometric method is developed and validated for the simultaneous estimation of some antiviral drugs such as emtricitabine (EMT), tenofovir disoproxil fumarate (TDF), and rilpivirine HCl (RPV) in tablet dosage form by Vierordt’s method. The solutions of standard and sample were prepared in methanol. The λmax⁡ for emtricitabine, tenofovir disoproxil fumarate, and rilpivirine hydrochloride were 240.8 nm, 257.6 nm, and 305.6 nm, respectively. Calibration curves are linear in the concentration ranges 4–12 μg/ml for EMT, 6–18 μg/ml for TDF, and 0.5–1.5 μg/ml for RPV, respectively. Results of analysis of simultaneous equation method were analyzed and validated for various parameters according to ICH guidelines.

scholarly journals Spectrophotometric quantitative analysis of metronidazole and itraconazole in a combined preparation made on the basis of Tizol gel

2020 ◽  
Vol 20 (5-6) ◽  
pp. 157-163
Author(s):  
Anna I. Zamaraeva ◽  
Natalya S. Bessonova ◽  
Tatyana A. Kobeleva ◽  
Alik I. Sichko
Keyword(s):  
Quantitative Analysis ◽  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Statistical Processing ◽  
Ultraviolet Region ◽  
System Of Equations ◽  
Base Materials

Actuality. Nowadays, the problems of effectiveness and accessibility of dermatoprotective therapy and prevention of dermatological diseases are urgent. The complex use of metronidazole in combination with drugs of other pharmacological groups is particularly relevant and promising. The dosage form consisting of 0.1 g of Itraconazole, 0.1 g of metronidazole and Tizol gel up to 10 g, termed by us Metroitraconazole, can be used in dermatology, ophthalmology and gynecology as a bactericidal and antifungal agent. The aim of the study is to develop the method for the quantitative spectrophotometric determination of metronidazole and itraconazole in a soft dosage form on a titanium-containing base. Materials and methods. For the analysis, we used substances, ethanol solutions of Metronidazole and Itraconazole, an ointment with the conditional name Metroitraconazole, containing 1.0% of the preparations in the Tizol gel. The study was carried out by spectrophotometry in the ultraviolet region, using spectrophotometer SF-2000 (Russia). Results. The study of the absorption spectra and statistical processing of the finding demontstrated that spectrophotometric determination of Itraconazole and metronidazole demanded the wavelengths of 262 and 312 nm, with a relative error of 1.52% and 1.67%, respectively. As a result of the analysis of the soft dosage form, it was determined that the content of metronidazole calculated with the use of Firordt method and a simplified system of equations ranged 0.0987-0.1057 g, and Itraconazole ranged 0.0925-0.1055 g. These data conformed the acceptance criteria. Conclusion. The conducted research allowed us to develop and propose a method for the quantitative determination of itraconazole and metronidazole in Metroitraconazole ointment by means of spectrophotometric method. It allowed us to determine the content of drugs in the dosage form with an error not exceeding the standard deviations.

Ultraviolet Spectrophotometric Determination of Hydralazine Hydrochloride in Tablets Following Derivatization with Nitrite

1987 ◽  
Vol 70 (1) ◽  
pp. 42-46
Author(s):  
Barry Mopper
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Absorption Maximum ◽  
Spectral Characteristics ◽  
Spectrophotometric Assay ◽  
Assay Method ◽  
Content Uniformity ◽  
Acidic Conditions ◽  
Hydralazine Hydrochloride

Abstract Routine use of the USP XXI spectrophotometric method for the content uniformity determination of hydralazine hydrochloride tablets has shown that tablet excipients can significantly alter the spectral characteristics of the drug and thus cause inaccurate assay values to be obtained. Because of this problem, a simple and reliable alternative spectrophotometric assay method, based on the conversion of hydralazine to tetrazolo [5,l-α]phthalazine with nitrite ions under acidic conditions, was developed. The derivative showed an absorption maximum at about 274 nm and obeyed Beer's law over the concentration range 4-40 μg/mL. Mean recoveries of hydralazine hydrochloride added to commercial coated and uncoated tablets were 101.0% (n = 10) and 100.8% (n = 8), respectively. The proposed method was found suitable for the assay not only of individual tablets but also of tablet composites

scholarly journals Quantification of Rifaximin in Tablets by Spectrophotometric Method Ecofriendly in Ultraviolet Region

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Ana Carolina Kogawa ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Correlation Coefficients ◽  
Environmentally Friendly ◽  
Analytical Techniques ◽  
Ultraviolet Region ◽  
Green Method ◽  
Detection And Quantification ◽  
Routine Quality Control

Rifaximin is an oral nonabsorbable antibiotic that acts locally in the gastrointestinal tract with minimal systemic adverse effects. It does not have spectrophotometric method ecofriendly in the ultraviolet region described in official compendiums and literature. The analytical techniques for determination of rifaximin reported in the literature require large amount of time to release results and are significantly onerous. Furthermore, they use toxic reagents both for the operator and environment and, therefore, cannot be considered environmentally friendly analytical techniques. The objective of this study was to develop and validate an ecofriendly spectrophotometric method in the ultraviolet region to quantify rifaximin in tablets. The method was validated, showing linearity, selectivity, precision, accuracy, and robustness. It was linear over the concentration range of 10–30 mg L−1with correlation coefficients greater than 0.9999 and limits of detection and quantification of 1.39 and 4.22 mg L−1, respectively. The validated method is useful and applied for the routine quality control of rifaximin, since it is simple with inexpensive conditions and fast in the release of results, optimizes analysts and equipment, and uses environmentally friendly solvents, being considered a green method, which does not prejudice either the operator or the environment.

scholarly journals UV Spectrophotometric Method for the Estimation of Tadalafil in Bulk and Tablet Dosage form

2010 ◽  
Vol 7 (3) ◽  
pp. 833-836 ◽  
Author(s):  
Mohammad Yunoos ◽  
D.Gowri Sankar ◽  
B.Pragati Kumar ◽  
Shahul Hameed
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Dosage Forms ◽  
Form Solution ◽  
Solid Dosage ◽  
Percentage Recovery ◽  
Accuracy And Precision ◽  
Tablet Dosage

A simple, sensitive, precise and highly accurate UV spectrophotometric method has been developed for the determination of tadalafil in bulk and tablet dosage form. Solution of tadalafil in methanol shows maximum absorbance at 284 nm. Beer’s law was obeyed in the concentration range of 2-20 mcg mL-1with 1.65x104mol-1cm-1, the slope, intercept, correlation coefficient, detection and quantitation limits were also calculated. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The method was validated by determining its sensitivity, accuracy and precision which proves suitability of the developed method for the routine estimation of tadalafil in bulk and solid dosage form.

scholarly journals UV Spectrophotometric Method for the Estimation of Valacyclovir HCl in Tablet Dosage Form

2009 ◽  
Vol 6 (3) ◽  
pp. 814-818 ◽  
Author(s):  
M. Ganesh ◽  
C. V. Narasimharao ◽  
A. Saravana Kumar ◽  
K. Kamalakannan ◽  
M. Vinoba ◽  
...  
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Dosage Forms ◽  
Form Solution ◽  
Solid Dosage ◽  
Percentage Recovery ◽  
Accuracy And Precision ◽  
Tablet Dosage

A simple, sensitive, highly accurate UV spectrophotometric method has been developed for the determination of valacyclovir in bulk and tablet dosage form. Solution of valacyclovir in 0.1N HCl shows maximum absorbance at 255 nm. Beer’s law was obeyed in the concentration range of 5-25 mcg mL-1with 1.0910x104mol-1cm-1, the slope, intercept, correlation coefficient, detection and quantitation limits were also calculated. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The percentages assay of valacyclovir HCl in tablet was 99.82%. The method was validated by determining its sensitivity, accuracy and precision which proves suitability of the developed method for the routine estimation of valacyclovir in bulk and solid dosage form.

scholarly journals Simultaneous determination of chloramphenicol and hydrocortisone acetate in cream using TLC desitrometry method

2012 ◽  
Vol 2 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Nia Kristiningrum ◽  
Mia Rakhmawati
Keyword(s):  
Quality Control ◽  
Mobile Phase ◽  
Limit Of Detection ◽  
Pharmaceutical Journal ◽  
Hydrocortisone Acetate ◽  
Limit Of Quantification ◽  
Quality Control Testing ◽  
Label Claim ◽  
Routine Quality Control

A rapid and reproducible TLC method was developed for the determination of hydrocortisone acetate and chloramphenicol in cream. The analytes were dissolved with methanol and chromatographed on silica Gel GF 254 TLC plate using chloroform:ethyl acetate in the ratio of 1:1.5 (v/v) as mobile phase. Spots at Rf 0.29 and Rf 0.59 were recognized as chloramphenicol and hydrocortisone acetate, respectively. Quantitative analysis was done through densitometric measurement at wavelength 265 nm. Method was found linear over the concentration range of 300-900 ng/spot with the correlation coefficient of 0.999 and 0.998 for hydrocortisone acetate and chloramphenicol, respectively. Specificity showed calculation of purity and identity more than 0.99. The limit of detection (LOD) and the limit of quantification (LOQ) of the method were 23.84 and 71.51 ng/spot for hydrocortisone acetate, 21.06 and 63.18 ng/spot for chloramphenicol. The precision of this method was less than 2.8% whereas the means of the recovery data were 100.40± 0.579% for hydrocortisone acetate and 100.24±1.20% for chloramphenicol. The proposed method has been applied to the determination of hydrocortisone acetate and chloramphenicol in commercial cream formulations and the recovery of label claim were 99.23±0.66% (chloramphenicol) and 99.25±0.41% (hydrocortisone acetate) for brand A and 100.32±0.87% (chloramphenicol) and 100.53±0.78% (hydrocortisone acetate) for brand B. The developed method was successfully used for the assay of hydrocortisone acetate and chloramphenicol. The method is simple, sensitive and precise; it can be used for the routine quality control testing of marketed formulations.DOI: http://dx.doi.org/10.3329/icpj.v2i1.12871 International Current Pharmaceutical Journal 2012, 2(1): 7-10 

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