scholarly journals Spectrophotometric quantitative analysis of metronidazole and itraconazole in a combined preparation made on the basis of Tizol gel

2020 ◽  
Vol 20 (5-6) ◽  
pp. 157-163
Author(s):  
Anna I. Zamaraeva ◽  
Natalya S. Bessonova ◽  
Tatyana A. Kobeleva ◽  
Alik I. Sichko
Keyword(s):  
Quantitative Analysis ◽  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Statistical Processing ◽  
Ultraviolet Region ◽  
System Of Equations ◽  
Base Materials

Actuality. Nowadays, the problems of effectiveness and accessibility of dermatoprotective therapy and prevention of dermatological diseases are urgent. The complex use of metronidazole in combination with drugs of other pharmacological groups is particularly relevant and promising. The dosage form consisting of 0.1 g of Itraconazole, 0.1 g of metronidazole and Tizol gel up to 10 g, termed by us Metroitraconazole, can be used in dermatology, ophthalmology and gynecology as a bactericidal and antifungal agent. The aim of the study is to develop the method for the quantitative spectrophotometric determination of metronidazole and itraconazole in a soft dosage form on a titanium-containing base. Materials and methods. For the analysis, we used substances, ethanol solutions of Metronidazole and Itraconazole, an ointment with the conditional name Metroitraconazole, containing 1.0% of the preparations in the Tizol gel. The study was carried out by spectrophotometry in the ultraviolet region, using spectrophotometer SF-2000 (Russia). Results. The study of the absorption spectra and statistical processing of the finding demontstrated that spectrophotometric determination of Itraconazole and metronidazole demanded the wavelengths of 262 and 312 nm, with a relative error of 1.52% and 1.67%, respectively. As a result of the analysis of the soft dosage form, it was determined that the content of metronidazole calculated with the use of Firordt method and a simplified system of equations ranged 0.0987-0.1057 g, and Itraconazole ranged 0.0925-0.1055 g. These data conformed the acceptance criteria. Conclusion. The conducted research allowed us to develop and propose a method for the quantitative determination of itraconazole and metronidazole in Metroitraconazole ointment by means of spectrophotometric method. It allowed us to determine the content of drugs in the dosage form with an error not exceeding the standard deviations.

scholarly journals Difference spectrophotometric method for the determination of Fluphenazine hydrochloride in tablets using peroxomonosulfate

2021 ◽  
Vol 9 (2) ◽  
pp. 64-71
Author(s):  
Mykola Blazheyevskiy ◽  
◽  
Valeriy Moroz ◽  
Olena Mozgova ◽  
◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Present Method ◽  
Molar Absorptivity ◽  
Limit Of Quantification ◽  
Derivatizing Agent ◽  
Sensitive Method

The oxidative derivatization method using potassium hydrogenperoxomonosulfate for the indirect spectrophotometric determination of Fluphenazine hydrochloride is presented. Potassium hydrogenperoxomonosulfate is introduced as a derivatizing agent for Fluphenazine hydrochloride, yielding the sulfoxide. This reaction product was successfully used for the spectrophotometric determination of the Fluphenazine hydrochloride. The UV spectroscopic detection of the sulfoxide proved to be a more robust and sensitive method. The elaborated method allowed the determination of Fluphenazine hydrochloride in the concentration range of 0.2-30 µg mL-1. The molar absorptivity at 349 nm is 5.6×103 (dm3cm-1mol-1). The limit of quantification, LOQ (10S) is 0.24 µg/mL. A new spectrophotometric technique was developed and the possibility of quantitative determination of Fluphenazine hydrochloride in tablets 5.0 mg was demonstrated. The present method is precise, accurate and excipients did not interfere. RSD for Fluphenazine Hydrochloride 5.0 mg tablets was 1.37 %.

scholarly journals The quantitative determination of Perphenazine in tablets by the spectrophotometric method as its sulfoxide obtained with diperoxyazelaic acid

2019 ◽  
Vol 7 (2) ◽  
pp. 96-103
Author(s):  
Mykola Blazheyevskiy ◽  
Myhailo Kucher ◽  
Oleh Shpychak
Keyword(s):  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Present Method ◽  
Derivatizing Agent

The oxidative derivatization method using diperoxyazelaic acid for the indirect spectrophotometric determination of Perphenazine dihydrochloride is presented. Diperoxyazelaic acid is introduced as a derivatizing agent for Perphenazine, yielding sulfoxides. This reaction product was successfully employed for the spectrophotometric determination of Perphenazine dihydrochloride. The UV spectroscopic detection of sulfoxide has been proven to be the more robust and selective. The method developed allowed determination of Perphenazine dihydrochloride in the concentration range of 1–40 µg/mL. The limits of quantification (LOQ=10S) is 3.3 µg·ml-1. A new spectrophotometric method has been developed, and the possibility of the quantitative determination of Perphenazine dihydrochloride in Perphenazine Tablets has been demonstrated. The present method is precise, accurate and other inactive excipients of the drug do not interfere. RSD = 2.00%; δ=( -µ) 100%/µ = – 0.85 %).

scholarly journals Quantitative determination of Levomepromazine in pharmaceuticals by spectrophotometric method as its sulfoxide

2020 ◽  
Vol 8 (1) ◽  
pp. 117-124
Author(s):  
Olena Mozgova ◽  
Mykola Blazheyevskiy
Keyword(s):  
Sodium Chloride ◽  
Citric Acid ◽  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Present Method ◽  
Limit Of Quantification ◽  
Derivatizing Agent ◽  
Sensitive Method

The oxidative derivatization method using Diperoxyazelaic acid for the indirect spectrophotometric determination of Levomepromazine hydrochloride is presented. Diperoxyazelaic acid is introduced as a derivatizing agent for Levomepromazine, yielding the sulfoxides. This reaction product was successfully used for the spectrophotometric determination of the Levomepromazine hydrochloride. The UV spectroscopic detection of the sulfoxide proved to be a more robust and sensitive method. The elaborated method allowed the determination of Levomepromazine hydrochloride in the concentration range of 3-150 µg/mL. The limit of quantification, LOQ (10S) is 2.85 µg/mL. A new spectrophotometric technique was developed and the possibility of quantitative determination of Levomepromazine in Tisercin Solution for Injection 25mg/mL was demonstrated. The present method is precise, accurate and other excipients: anhydrous citric acid, monothioglycerol, sodium chloride did not interfere. RSD = 1.24 % (δ = –0.02 %).

scholarly journals Spectrophotometric Determination of Chlorpropamide in Bulk and Dosage Form by Complexation with Chloranilic Acid

2010 ◽  
Vol 3 (1) ◽  
pp. 207
Author(s):  
C. J. Mbah ◽  
N. H. Okorie
Keyword(s):  
Charge Transfer ◽  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Electron Donor ◽  
Dosage Form ◽  
Spectrophotometric Study ◽  
Chloranilic Acid ◽  
Coloured Complex ◽  
Charge Transfer Complexation

A spectrophotometric study of chlorpropamide is described. The method is based on the charge-transfer complexation between chlorpropamide (the n-electron donor) with chloranilic acid (the p-acceptor) to form a violet coloured complex having absorption maxima at 530 nm. Beer’s law is obeyed for up to 5-25 mg/ml of chlorpropamide. Results of the analysis of this method were validated statistically by recovery studies. The proposed method is simple, accurate and precise for the quantitative determination of chlorpropamide in bulk and tablet formulations.Keywords: Chlorpropamide; Spectrophotometry; Chloranilic acid.© 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi:10.3329/jsr.v3i1.4808                 J. Sci. Res. 3 (1), 207-212 (2011)

Spectrophotometric Determination of Ethchlorvynol in Biologic Specimens

1974 ◽  
Vol 20 (2) ◽  
pp. 159-162 ◽  
Author(s):  
Jack E Wallace ◽  
Horace E Hamilton ◽  
Joel A Riloff ◽  
Kenneth Blum
Keyword(s):  
Quantitative Determination ◽  
Ultraviolet Light ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Product Mixture

Abstract A sensitive, highly specific spectrophotometric method for quantitative determination of ethchlorvynol in biologic specimens is described. The procedure is based on conversion of ethchlorvynol to a product mixture consisting of two major components that strongly absorb ultraviolet light. Results can be obtained within 45 min of receipt of a specimen.

scholarly journals Development of a Method of Analysis of Ofloxacin in the Complex Preparation "Ofloxazol"

2021 ◽  
Vol 10 (3) ◽  
pp. 70-75
Author(s):  
T. A. Kobeleva ◽  
A. I. Sichko ◽  
A. I. Zamaraeva ◽  
N. S. Bessonova
Keyword(s):  
Quantitative Determination ◽  
Dosage Form ◽  
Inflammatory Diseases ◽  
Statistical Processing ◽  
Chemotherapeutic Agents ◽  
Spectrophotometric Analysis ◽  
Uv Spectrophotometry ◽  
Treatment And Prevention ◽  
Complex Preparation

Introduction. The creation of new effective antibacterial drugs for the treatment and prevention of purulent-inflammatory diseases is an urgent task of modern pharmacy. Active use in the treatment of purulent infection is found by chemotherapeutic agents from the class of fluoroquinolones, which include ofloxacin.Aim. Development of a method for the quantitative determination of ofloxacin in the complex preparation "Ofloxazol".Materials and methods. For the analysis, the substance ofloxacin, titanium-containing gel "Tizol", solutions of ofloxacin on 95 % ethanol, hydrochloric acid 0.01 mol/l, ointment under the conditional name "Ofloxazol" containing 0.5 % of the drug in the gel "Tizol" were used. The study was carried out by near-UV spectrophotometry.Results and discussion. When studying the absorption spectra, it was found that for the quantitative spectrophotometric analysis of ofloxacin, it is rational to use the wavelength range of 275-320 nm (λmax = 294 nm). Statistical processing of the analysis results showed that the relative error of quantitative determination does not exceed ±1.66 %. The sensitivity of the determination of ofloxacin is 0.245 mcg/ml at A(min) = 0.02. The developed method is validated. Its specificity, linearity, correctness and precision are confirmed. According to the calibration schedule, the content of ofloxacin in the soft dosage form is determined, it is in the range of 0.0483-0.0562 g, which corresponds to the permissible deviations.Conclusion. The conducted studies allowed us to develop and propose a method for the quantitative determination of ofloxacin in the ointment "Ofloxazol", obtained on a titanium-containing basis. The method allows you to evaluate the quality of manufacturing the dosage form, including setting the content of the drug with an error that does not exceed the standard deviations.

scholarly journals Simple UV Spectrophotometric Determination of Rosuvastatin Calcium in Pure Form and in Pharmaceutical Formulations

2009 ◽  
Vol 6 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Alka Gupta ◽  
P. Mishra ◽  
K. Shah
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pure Form ◽  
Ultraviolet Region ◽  
Rosuvastatin Calcium ◽  
Sensitive Spectrophotometric Method

A new, simple and sensitive spectrophotometric method in ultraviolet region has been developed for the determination of rosuvastatin calcium in bulk and in pharmaceutical formulations. Rosuvastatin exhibits absorption maxima at 244 nm with apparent molar absorptivity of 7.2345 ×104L/mol.cm in methanol. Beer’s law was found to be obeyed in the concentration range of 2-18 µg/mL. The method is accurate, precise and economical. This method is extended to pharmaceutical preparations. In this method, there is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated statistically and by recovery studies

scholarly journals Development and validation of aranosa assay in the dosage form

2018 ◽  
Vol 17 (2) ◽  
pp. 57-62 ◽  
Author(s):  
Z. S. Shprakh ◽  
E. V. Ignаteva ◽  
I. V. Yartseva
Keyword(s):  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Active Substance ◽  
Dosage Form ◽  
Sorbic Acid ◽  
Drug Dosage ◽  
Acid Method ◽  
Drug Dosage Form ◽  
Development And Validation

Background. Aranosa, a drug of nitrosoalkylureas class, was developed and studied at N.N. Blokhin NMRCO, Russia, and at present it is produced by N.N. Blokhin NMRСO branch “Naukoprofi”. One of the stages of the drug standardization is the development of an assay technique for quantitative determination of active substance in the final drug dosage form.Objective. Development and validation of an assay for quantitative determination of Aranosa in the dosage form.Materials and methods. The study used “Aranosa, lyophilisate for solution for injection 0.5 g”; Аranosa, substance-powder (N.N. Blokhin NMRCO branch “Naukoprofi”); polyvinylpyrrolidone low-molecular medical; sorbic acid. Method: spectrophotometry. Results. An assay was developed for quantitative spectrophotometric determination of Aranosa in the dosage form “Aranosa, lyophilisate for solution for injection 0.5 g”. Validation was performed to prove reliability and accuracy of the obtained results. The assay was evaluated by validation characteristics, such as specificity, linearity, trueness, precision.Conclusions. The developed assay is provided with trueness, repeatability, precision, and linearity and can be used in the range of 80– 120 % of nominal Aranosa content in the dosage form.

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