Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector

We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner. Single transfection with pCRTEIL resulted in predominant expression of red fluorescence. Cotransfection with pCRTEIL and Cre-expression plasmid (pCAG/NCre) caused switching from red to green fluorescence. Similarly, cotransfection with pCETRIZ and pCAG/NCre resulted in change of green to red fluorescence. These noninvasive systems will be useful in cell lineage analysis, since descendants of cells exhibiting newly activated gene expression can be continuously monitored in noninvasive fashion.

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StemMapper: a curated gene expression database for stem cell lineage analysis

Nucleic Acids Research ◽

10.1093/nar/gkx921 ◽

2017 ◽

Vol 46 (D1) ◽

pp. D788-D793 ◽

Cited By ~ 9

Author(s):

José P Pinto ◽

Rui S R Machado ◽

Ramiro Magno ◽

Daniel V Oliveira ◽

Susana Machado ◽

...

Keyword(s):

Gene Expression ◽

Stem Cell ◽

Cell Lineage ◽

Gene Expression Database ◽

Stem Cell Lineage ◽

Lineage Analysis

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Faculty Opinions recommendation of Cell lineage analysis of the amphipod crustacean Parhyale hawaiensis reveals an early restriction of cell fates.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1011903.183314 ◽

2003 ◽

Author(s):

Susan Brown

Keyword(s):

Cell Lineage ◽

Cell Fates ◽

Parhyale Hawaiensis ◽

Amphipod Crustacean ◽

Lineage Analysis

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Major sex-determining genes and the control of sexual dimorphism in Caenorhabditis elegans

Genome ◽

10.1139/g89-116 ◽

1989 ◽

Vol 31 (2) ◽

pp. 625-637 ◽

Cited By ~ 4

Author(s):

Jonathan Hodgkin ◽

Andrew D. Chisholm ◽

Michael M. Shen

Keyword(s):

Caenorhabditis Elegans ◽

Sex Determination ◽

X Chromosome ◽

Germ Line ◽

Cell Lineage ◽

Regulatory Genes ◽

Nematode Caenorhabditis Elegans ◽

X Chromosomes ◽

The Many ◽

Lineage Analysis

Sex determination in Caenorhabditis elegans involves a cascade of major regulatory genes connecting the primary sex determining signal, X chromosome dosage, to key switch genes, which in turn direct development along either male or female pathways. Animals with one X chromosome (XO) are male, while animals with two X chromosomes (XX) are hermaphrodite: hermaphrodite development occurs because the action of the regulatory genes is modified in the germ line so that both sperm and oocytes are made inside a completely female soma. The regulatory genes are being examined by both genetic and molecular means. We discuss how these major genes, in particular the last switch gene in the cascade, tra-1, might regulate the many different sex-specific events that occur during the development of the hermaphrodite and of the male.Key words: nematode, Caenorhabditis elegans, sex determination, sexual differentiation, cell lineage analysis.

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Cell lineage of homeotic mutants of Drosophila

Philosophical Transactions of the Royal Society of London Series B Biological Sciences ◽

10.1098/rstb.1985.0183 ◽

1985 ◽

Vol 312 (1153) ◽

pp. 139-144 ◽

Cited By ~ 1

Keyword(s):

Mode Of Action ◽

Cell Lineage ◽

Precursor Cells ◽

Larval Period ◽

Homeotic Genes ◽

Lineage Analysis

The homeotic genes specify the development of specific groups of precursor cells. They establish the correct state of determination of the different primordia. Cell lineage analysis has been particularly useful in studying the mode of action of homeotic genes. The main findings are: (i) most, perhaps all, the homeotic genes are required by every cell of the corresponding primordium (that is, they are cell autonomous); (ii) they act on anatomical units defined by compartment boundaries and including one or more compartments, (iii) most, but not all, homeotic genes are required until the end of the larval period; (iv) the homeotic genes act in combination so that the appropriate development of a given primordium may be established by the contribution of several homeotic genes.

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Feedback-Induced Variations of Distribution in a Representative Gene Model

International Journal of Bifurcation and Chaos ◽

10.1142/s0218127415400088 ◽

2015 ◽

Vol 25 (07) ◽

pp. 1540008

Author(s):

Peijiang Liu ◽

Zhanjiang Yuan ◽

Lifang Huang ◽

Tianshou Zhou

Keyword(s):

Gene Expression ◽

Power Law ◽

Positive Feedback ◽

Bimodal Distribution ◽

Stochastic Bifurcation ◽

Protein Distribution ◽

Identical Cell ◽

Induced Changes ◽

Gene Switching ◽

Cell To Cell Variability

Gene expression is inherently noisy, implying that the number of mRNAs or proteins is not invariant rather than follows a distribution. This distribution can not only provide the exact information on the dynamics of gene expression but also describe cell-to-cell variability in a genetically identical cell population. Here, we systematically investigate a two-state model of gene expression, a model paradigm used to study expression dynamics, focusing on the effect of feedback on the type of mRNA or protein distribution. If there is no feedback, then the distribution may be bimodal, power-law tailed, or Poisson-like, depending on gene switching rates. However, we find that feedback can tune or change the type of the distribution in each case and tends to unimodalize the distribution as its strength increases. Specifically, positive feedback can change not only a power-law tailed distribution into a bimodal or Poisson-like distribution but also a bimodal distribution into a Poisson-like distribution (implying that stochastic bifurcation can take place). In addition, it can make a Poisson-like distribution become more peaked but does not change the type of this distribution. In contrast to positive feedback, negative feedback has less influence on the shape of the distributions except for the bimodal case. In all cases, the noise-feedback curve used extensively in previous studies cannot well reflect the feedback-induced changes in the shape of distributions. Feedback-induced variations in distribution would be important for cell survival in fluctuating environments.

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Cell lineage analysis in neural crest ontogeny

Journal of Neurobiology ◽

10.1002/neu.480240203 ◽

1993 ◽

Vol 24 (2) ◽

pp. 146-161 ◽

Cited By ~ 74

Author(s):

Nicole M. Le Douarin ◽

Elisabeth Dupin

Keyword(s):

Neural Crest ◽

Cell Lineage ◽

Lineage Analysis

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ming is expressed in neuroblast sublineages and regulates gene expression in the Drosophila central nervous system

Development ◽

10.1242/dev.116.4.943 ◽

1992 ◽

Vol 116 (4) ◽

pp. 943-952 ◽

Cited By ~ 8

Author(s):

X. Cui ◽

C.Q. Doe

Keyword(s):

Gene Expression ◽

Central Nervous System ◽

Nervous System ◽

Cell Fate ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Cell Lineage ◽

Cell Lineages ◽

Finger Protein ◽

Cell Diversity

Cell diversity in the Drosophila central nervous system (CNS) is primarily generated by the invariant lineage of neural precursors called neuroblasts. We used an enhancer trap screen to identify the ming gene, which is transiently expressed in a subset of neuroblasts at reproducible points in their cell lineage (i.e. in neuroblast ‘sublineages’), suggesting that neuroblast identity can be altered during its cell lineage. ming encodes a predicted zinc finger protein and loss of ming function results in precise alterations in CNS gene expression, defects in axonogenesis and embryonic lethality. We propose that ming controls cell fate within neuroblast cell lineages.

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Genetic analysis of leaf development in cotton

Development ◽

10.1242/dev.113.supplement_1.39 ◽

1991 ◽

Vol 113 (Supplement_1) ◽

pp. 39-46 ◽

Cited By ~ 1

Author(s):

Liam Dolan ◽

R. Scott Poethig

Keyword(s):

Leaf Growth ◽

Leaf Shape ◽

Cell Lineage ◽

Cotton Leaf ◽

Expansion Phase ◽

Genetic Mosaics ◽

Developmental Age ◽

Allometric Analysis ◽

Marginal Region ◽

Lineage Analysis

Leaf shape in cotton is regulated by the developmental age of the shoot and by several major genes that affect leaf lobing. The effect of these factors was investigated by allometric analysis, cell lineage analysis, and by studying the expression of the leaf shape mutation, Okra, in genetic mosaics. Allometric analysis of leaf growth suggests that leaf shape is determined during the initiation of the primordium rather than during the expansion phase of leaf growth. Clonal analysis demonstrates that both the rate and duration of cell division are fairly uniform throughout the leaf. Cells in the marginal region of the developing cotton leaf contribute more to the growth of the lamina than they do in tobacco. The Okra mutation acts early in the development of a leaf and appears to accentuate a developmental pattern that is also responsible for heteroblastic variation in leaf shape. The expression of this mutation in genetic mosaics demonstrates that its effect does not diffuse laterally within the leaf primordium.

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