Creating a Long-Term Diabetic Rabbit Model

This study was to create a long-term rabbit model of diabetes mellitus for medical studies of up to one year or longer and to evaluate the effects of chronic hyperglycemia on damage of major organs. A single dose of alloxan monohydrate (100 mg/kg) was given intravenously to 20 young New Zealand White rabbits. Another 12 age-matched normal rabbits were used as controls. Hyperglycemia developed within 48 hours after treatment with alloxan. Insulin was given daily after diabetes developed. All animals gained some body weight, but the gain was much less than the age-matched nondiabetic rabbits. Hyperlipidemia, higher blood urea nitrogen and creatinine were found in the diabetic animals. Histologically, the pancreas showed marked beta cell damage. The kidneys showed significantly thickened afferent glomerular arterioles with narrowed lumens along with glomerular atrophy. Lipid accumulation in the cytoplasm of hepatocytes appeared as vacuoles. Full-thickness skin wound healing was delayed. In summary, with careful management, alloxan-induced diabetic rabbits can be maintained for one year or longer in reasonably good health for diabetic studies.

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Electrospinning of Poly(ethylene-co-vinyl alcohol) Nanofibres Encapsulated with Ag Nanoparticles for Skin Wound Healing

Journal of Nanomaterials ◽

10.1155/2011/201834 ◽

2011 ◽

Vol 2011 ◽

pp. 1-7 ◽

Cited By ~ 22

Author(s):

Chao Xu ◽

Feng Xu ◽

Bin Wang ◽

TianJian Lu

Keyword(s):

Wound Healing ◽

Ag Nanoparticles ◽

Vinyl Alcohol ◽

Skin Wound ◽

Electrospinning Technique ◽

Skin Wound Healing ◽

Electric Voltage ◽

Donor Skin ◽

Poly Ethylene

Skin wound healing is an urgent problem in clinics and military activities. Although significant advances have been made in its treatment, there are several challenges associated with traditional methods, for example, limited donor skin tissue for transplantation and inflammation during long-term healing time. To address these challenges, in this study we present a method to fabricate Poly(ethylene-co-vinyl alcohol) (EVOH) nanofibres encapsulated with Ag nanoparticle using electrospinning technique. The fibres were fabricated with controlled diameters (59 nm–3 μm) by regulating three main parameters, that is, EVOH solution concentration, the electric voltage, and the distance between the injection needle tip (high-voltage point) and the fibre collector. Ag was added to the nanofibres to offer long-term anti-inflammation effect by slow release of Ag nanoparticles through gradual degradation of EVOH nanofibre. The method developed here could lead to new dressing materials for treatment of skin wounds.

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Management of Diffuse Necrotic Cutaneous Wound in a Dog

Sahel Journal of Veterinary Sciences ◽

10.54058/saheljvs.v17i2.133 ◽

2020 ◽

Vol 17 (2) ◽

pp. 49-52

Author(s):

S. T. Muhammad ◽

M. Abdurrahman ◽

D. O. Avazi ◽

B. Usman ◽

E. G. Emmanuel ◽

...

Keyword(s):

Wound Healing ◽

Skin Wound ◽

Initial Assessment ◽

Adhesive Tape ◽

Skin Wound Healing ◽

Management Intervention ◽

Wound Swab ◽

One Year ◽

The Right ◽

Systemic Antibiotics

A one-year-old male Terrier dog weighing 22 kg was presented to the Ahmadu Bello University Veterinary Teaching Hospital (ABUVTH), Zaria, Nigeria, with complaints of sloughing of the skin and purulent discharges from an injured (sloughed) shoulder. Clinical examination revealed normal vital parameters, extensive necrotized skin on the dorsum extending from loin to the right shoulder and enlarged superficial lymph nodes. Blood and wound swab samples were evaluated in the clinical haematology and Microbiology Laboratories of the ABUVTH, respectively. The Pathology result showed leucocytosis due to neutrophilia and monocytosis. Staphylococcus aureus was isolated from the wound swab. The wound was managed by wet-to-dry bandaging technique using topical antiseptics (Para-chloroaniline solution, chlorhexidine and povidone iodine), improvised non-adhesive protective membrane (polyethylene) applied at the wound-bandage interface, bandages along with adhesive tape and systemic antibiotics. Healing occurred uneventfully within 6 weeks of the intervention. The paper discusses the detail of the management initiated and how it greatly influenced the skin wound healing in dogs. In conclusion, proper initial assessment of wounds and appropriate treatment through rigorous wound assessment and bandaging are critical to success in the wound healing and restoration of tissue integrity. This management intervention could be further investigated in animals in order to improve the quality of cutaneous wounds management.

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Cardiac function profiling and its antidiabetic effect of Daphne mucronata in alloxan induced diabetic rabbits

JOURNAL OF BOTANY ◽

10.33195/uochjb-v1i1382017 ◽

2017 ◽

pp. 125-131

Author(s):

BASIT ALI

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Cardiac Function ◽

Herbal Remedies ◽

Elevated Plasma ◽

Antidiabetic Effect ◽

Chronic Hyperglycemia ◽

Daphne Mucronata ◽

Diabetic Rabbits ◽

Alloxan Monohydrate

Diabetes mellitusis a chronic hyperglycemia associated with elevated plasma cholesterol.Natural and traditional herbal remedies are a source of new commercial products. Anti-diabetic potential of D. mucronata extracts (methanol) has been assessed in alloxan monohydrate diabetic induced rabbits.The extracts were given orally by 8 hours. Thedose rate was 12 mg, 125mg, 225 mg and 325mg/kg body weight respectively.At every 2 hours blood sample was collected, serum separated and glucose level was determined by kit method. The result suggests that the treatment of 325 mg/kg body weight significantly(p<0.05) decreased blood glucose and cholesterol level at 143 mg/dl and 166.33 mg/dl in the experimental group.Therefore, it is concluded that the D. mucronata possess significant anti-diabetic activity.

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Histological studies of the effects of wounding vibrissa follicles in the hooded rat

Development ◽

10.1242/dev.83.1.95 ◽

1984 ◽

Vol 83 (1) ◽

pp. 95-108

Author(s):

Colin A. B. Jahoda ◽

Roy F. Oliver

Keyword(s):

Cell Damage ◽

Dermal Papilla ◽

Fibre Production ◽

Scar Tissue ◽

Hair Cycle ◽

Cell Counts ◽

History Of ◽

One Year ◽

Fibre Characteristics

The effects of wounding the lower region of rat vibrissa follicles with a sharp tungsten needle were examined histologically, both shortly after injury and up to one year postoperatively. Following cell damage in the dermal papilla component hair growth ceased, and resumption of fibre production was always preceded by dermal papilla reformation. This papilla healing and regeneration was not associated with the production of scar tissue. In follicles undergoing no cell displacement during wounding (an effect associated with the growth of longer than normal hairs) dermal papillae were reformed from the residual papilla cell population, with recruitment of cells from surrounding mesenchyme. Follicles plucked just prior to wounding revealed little or no original epidermal matrix three days later, confirming that dermal components were primarily affected. Papilla cell counts performed on follicles which had consistently produced longer hairs gave no indication of increased papilla cell numbers. Follicles which underwent displacement of cellular material and displayed distortion of normal follicle morphology shortly after wounding (effects associated with the production of shorter than normal hairs) also revealed abnormalities at long-term biopsy. Moreover these follicles often had a history of altered fibre characteristics from one postoperative generation to the next. It is concluded that gross morphological disruption of the normal cellular relationships in the lower follicle results in a series of reorganizational difficulties with each recurring phase of the hair cycle.

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P5473Perceived well-being after acute myocardial infarction according to diabetic status and its impact long-term mortality. The FAST-MI programme

European Heart Journal ◽

10.1093/eurheartj/ehz746.0427 ◽

2019 ◽

Vol 40 (Supplement_1) ◽

Author(s):

N Danchin ◽

E Puymirat ◽

V Bataille ◽

Y Cottin ◽

X Tabone ◽

...

Keyword(s):

Health Status ◽

Chest Pain ◽

Well Being ◽

Good Health ◽

Health Condition ◽

Diabetic Patients ◽

Diabetic Status ◽

Medication Side ◽

One Year

Abstract Background Little information is available regarding subjective well-being according to diabetic status in patients surviving an acute myocardial infarction (AMI). Aims and methods A health status questionnaire was sent to all patients participating in the nationwide French FAST-MI cohorts (2005, 2010 and 2015) one year after the acute episode of AMI, with a specific question on overall health condition (how do you feel: very well, well, fair, poor) (n=6082), and additional questions on the presence of any chest pain (n=4590), presence nuisance bleedings (n=3968), and reporting of perceived medication side-effects (n=2220). Answers were analysed according to diabetic status. We also analysed in the 2005 and 2010 cohorts whether subjective health status at one year was a correlate of 5-year mortality. Results Compared with non-diabetic patients (n=4692), those with diabetes (n=1390) had a poorer perceived health status (poor/fair health status: 40% vs 30%, P<0.001), more presence of chest pain (31% vs 23%, P<0.001), but less nuisance bleedings (47% vs 56%, P<0.001) and similar reported medication side-effects (37% vs 41%, P=0.12). Using logistic regression analysis, diabetes was an independent correlate of poorer health status (any diabetes OR vs non-diabetic 1.29, 1.13–1.49, P<0.001; non-insulin-treated: 1.17, 1.00–1.36, P=0.05, insulin-treated: 1.58, 1.29–1.94, P<0.001). In diabetic patients, perceived health condition at one year was an independent correlate of 5-year death (Cox multivariate analysis): compared with patients reporting very good health, HR (95% CI) 2.16 (1.08–4.32) for good health, 3.06 (1.53–6.11) for fair health, and 3.63 (1.55–8.55) for poor health; in non-diabetic patients, 5-year survival was similar in those reporting good or very good health status, but lower in those reporting fair or poor health status (Figure). Figure 1. 5-year survival by health status Conclusion Compared with non-diabetic patients, patients with diabetes described a poorer general health and more residual chest pain after AMI, but had less nuisance bleedings. Health condition reported one year after AMI was an independent correlate of subsequent long-term mortality, particularly for diabetic patients. Acknowledgement/Funding Amgen, AstraZeneca, Bayer, Daiichi-Sankyo, Eli-Lilly, GSK, MSD, Novartis, Pfizer, Sanofi, Servier

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Regulated splicing of the fibronectin EDA exon is essential for proper skin wound healing and normal lifespan

The Journal of Cell Biology ◽

10.1083/jcb.200212079 ◽

2003 ◽

Vol 162 (1) ◽

pp. 149-160 ◽

Cited By ~ 193

Author(s):

Andrés F. Muro ◽

Anil K. Chauhan ◽

Srecko Gajovic ◽

Alessandra Iaconcig ◽

Fabiola Porro ◽

...

Keyword(s):

Wound Healing ◽

Alternative Splicing ◽

Skin Wound ◽

Mouse Strains ◽

Skin Wound Healing ◽

Alternatively Spliced ◽

Complete Exclusion ◽

Development And Aging

Fibronectins (FNs) are multifunctional high molecular weight glycoproteins present in the blood plasma and in the ECMs of tissues. The FN primary transcript undergoes alternative splicing in three regions generating up to 20 main different variants in humans. However, the precise role of the FN isoforms is poorly understood. One of the alternatively spliced exons is the extra domain A (EDA) or extra type III homology that is regulated spatially and temporally during development and aging. To study its in vivo function, we generated mice devoid of EDA exon-regulated splicing. Constitutive exon inclusion was obtained by optimizing the splice sites, whereas complete exclusion was obtained after in vivo CRE-loxP–mediated deletion of the exon. Homozygous mouse strains with complete exclusion or inclusion of the EDA exon were viable and developed normally, indicating that the alternative splicing at the EDA exon is not necessary during embryonic development. Conversely, mice without the EDA exon in the FN protein displayed abnormal skin wound healing, whereas mice having constitutive inclusion of the EDA exon showed a major decrease in the FN levels in all tissues. Moreover, both mutant mouse strains have a significantly shorter lifespan than the control mice, suggesting that EDA splicing regulation is necessary for efficient long-term maintenance of biological functions.

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