scholarly journals Thrombospondin-1 (TSP-1) Stimulates Expression of Integrinα6 in Human Breast Carcinoma Cells: A Downstream Modulator of TSP-1-Induced Cellular Adhesion

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Anitha S. John ◽  
Vicki L. Rothman ◽  
George P. Tuszynski
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Human Keratinocytes ◽  
Cellular Adhesion ◽  
Immunohistochemical Localization ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Thrombospondin 1

Thrombospondin-1 (TSP-1) is involved in a variety of different cellular processes including cell adhesion, tumor progression, and angiogenesis. This paper reports the novel finding that TSP-1 upregulates integrinα6 subunit in human keratinocytes and human breast cancer cells resulting in increased cell adhesion and tumor cell invasion. The effect of TSP-1 onα6 subunit expression was examined in human keratinocytes and breast adenocarcinoma cell lines (MDA-MB-231) treated with TSP-1 and in TSP-1 stably transfected breast cancer cells. TSP-1 upregulatedα6 message and protein in these cells as revealed by differential display, Northern and Western blot analysis and immunohistochemical localization studies. The increased expression ofα6 was shown to mediate adhesion and invasion of these cells to laminin, a major component of the basement membrane and extracellular matrix (ECM). These data suggest that TSP-1 plays an integral role in the attachment of cells to the ECM facilitating cell motility and angiogenesis.

The Impact of Antioxidants from the Diet on Breast Cancer Cells Monitored by Raman Microspectroscopy

2018 ◽  
Vol 16 (2) ◽  
pp. 127-137
Author(s):  
Paula Sofia Coutinho Medeiros ◽  
Ana Lúcia Marques Batista de Carvalho ◽  
Cristina Ruano ◽  
Juan Carlos Otero ◽  
Maria Paula Matos Marques
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Breast Adenocarcinoma ◽  
Coumaric Acid ◽  
Human Breast ◽  
The Impact

Background: The impact of the ubiquitous dietary phenolic compound p-coumaric acid on human breast cancer cells was assessed, through a multidisciplinary approach: Combined biological assays for cytotoxicity evaluation and biochemical profiling by Raman microspectroscopic analysis in cells. </P><P> Methods: Para-coumaric acid was shown to exert in vitro chemoprotective and antitumor activities, depending on the concentration and cell line probed: a significant anti-invasive ability was detected for the triple-negative MDA-MB-231 cells, while a high pro-oxidant effect was found for the estrogen- dependent MCF-7 cells. A striking cell selectivity was obtained, with a more noticeable outcome on the triple-negative MDA-MB-231 cell line. Results: The main impact on the cellular biochemical profile was verified to be on proteins and lipids, thus justifying the compound´s anti-invasive effect and chemoprotective ability. Conclusion: p-Coumaric acid was thus shown to be a promising chemoprotective/chemotherapeutic agent, particularly against the low prognosis triple-negative human breast adenocarcinoma.

scholarly journals Doxorubicin–transferrin conjugate alters mitochondrial homeostasis and energy metabolism in human breast cancer cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Paulina Wigner ◽  
Krzysztof Zielinski ◽  
Magdalena Labieniec-Watala ◽  
Agnieszka Marczak ◽  
Marzena Szwed
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Mitochondrial Function ◽  
Breast Cancer Cells ◽  
Chemotherapeutic Agents ◽  
Breast Adenocarcinoma ◽  
Breast Cancer Cell Lines ◽  
Antioxidant Systems ◽  
Exact Nature ◽  
Human Breast

AbstractDoxorubicin (DOX) is considered one of the most powerful chemotherapeutic agents but its clinical use has several limitations, including cardiomyopathy and cellular resistance to the drug. By using transferrin (Tf) as a drug carrier, however, the adverse effects of doxorubicin as well as drug resistance can be reduced. The main objective of this study was to determine the exact nature and extent to which mitochondrial function is influenced by DOX–Tf conjugate treatment, specifically in human breast adenocarcinoma cells. We assessed the potential of DOX–Tf conjugate as a drug delivery system, monitoring its cytotoxicity using the MTT assay and ATP measurements. Moreover, we measured the alterations of mitochondrial function and oxidative stress markers. The effect of DOX–Tf was the most pronounced in MDA-MB-231, triple-negative breast cancer cells, whereas non-cancer endothelial HUVEC-ST cells were more resistant to DOX–Tf conjugate than to free DOX treatment. A different sensitivity of two investigate breast cancer cell lines corresponded to the functionality of their cellular antioxidant systems and expression of estrogen receptors. Our data also revealed that conjugate treatment mediated free radical generation and altered the mitochondrial bioenergetics in breast cancer cells.

The Novel Method for Delivery miRNA-34a: a New Cationic PEGylated Niosomal Formulation for the Treatment of Breast Cancer

2021 ◽  
Author(s):  
Najmeh Alsadat Abtahi ◽  
Fateme Haghiralsadat ◽  
Abolghasem Siyadatpanah ◽  
Javad Zavar Reza ◽  
Veeranoot Nissapatorn ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
High Efficiency ◽  
Small Diameter ◽  
Spherical Shape ◽  
Cancer Drug ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Mcf 7

Abstract Background: The reactive surface of nanoparticles makes it possible to simply modify with a biocompatible coating and load with therapeutic agents such as siRNA, miRNA, an anti-cancer drug, and antibody. MicroRNAs, like the noncoding RNAs, contribute critical to the regulation of numerous cellular functions via transcriptional silencing. MicroRNAs (miRNAs) have enormous potential in cancer treatment, however, it is difficult to deliver them effectively to most solid tumors. The encapsulation of miRNA-34a in niosome nanoparticles is an attractive strategy for biopharmaceutical resources against cancer. The present study investigated the effectiveness of anticancer activity against MCF-7 and T47D human breast adenocarcinoma cells of a new noiosome system composed of nonionic surfactants. Methods: We used the optimum formulations to transfer miRNA-34a to breast cancer cells, providing potential benefits, such as exceptionally high entrapment efficiency (almost 100%), spherical shape, suitable positive charge (zeta potential~ + 24 mV) and small diameter (~100 nm). Results: The miRNA-34a-niosomes represented improved cytotoxic activity against the cancer cells compared to readily dispersed miRNA-34a. The resulting data indicate that delivery of miRNA-34a via niosome can affect tumor suppression, highlighting its promising anticancer effects in breast cancer cells. Conclusion: In conclusion, the developed a new carrier to improve the delivery of miRNA-34a into the tumor cells. The formulation provided in the present work is stable with a sustained release, high efficiency of miRNA-34a loading having a diameter of 115 nm. miRNA loading is performed without potentially harmful chemical reactions. Niosomes loaded with miRNA-34a in this study represented significant cytotoxic effects against the human breast cancer MCF-7 and T47D cells, which highlights their potential effect on this type of cancer cells.

Relaxin influences growth, differentiation and cell-cell adhesion of human breast-cancer cells in culture

1994 ◽  
Vol 57 (1) ◽  
pp. 129-134 ◽  
Author(s):  
Tatiana Bani Sacchi ◽  
Daniele Bani ◽  
Maria Luisa Brandi ◽  
Alberto Falchetti ◽  
Mario Bigazzi
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Cells In Culture ◽  
Cell Cell

scholarly journals FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 cells): Morphometric Analysis

2018 ◽  
Author(s):  
Firdos Alam Khan ◽  
Sultan Akhtar ◽  
Sarah Ameen Almofty ◽  
Dana Almohazey ◽  
Munthar Alomari
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Breast Adenocarcinoma ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Surgical Interventions ◽  
Mcf 7

Breast cancer treatment mostly revolved around radiation therapy and surgical interventions, these treatments doesn&rsquo;t provide satisfactory relief to the patients and carry unmanageable side-effects. Nanomaterials show promising results in treating cancer cells and have many advantages such as high biocompatibility, bioavailability and effective therapeutic capabilities. Interestingly, fluorescent magnetic nanoparticles have been used in many biological and diagnostic applications, but there is no report of use of fluorescent magnetic submicronic polymer nanoparticles (FMSP-nanoparticles) in the treatment of human breast cancer cells. In the present study, we have tested the effect FMSP-nanoparticles on human breast cancer cells (MCF-7). We have tested different concentrations (1.25&micro;g/1mL, 12.5&micro;g/mL and 50&micro;g/1mL) of FMSP-nanoparticles in MCF-7 cells and evaluated the nanoparticles response morphometrically. Our results revealed that FMSP-nanoparticles produced a concentration dependent effect on the cancer cells, dose of 1.25&micro;g/mL produced no significant effect on the cancer cell morphology and cell death, whereas dosages of 12.5&micro;g/mL and 50&micro;g/mL respectively showed significant nuclear augmentation, disintegration, chromatic condensation followed by dose dependent cell death. Our results demonstrate FMSP-nanoparticles have ability to induce cell death in MCF-7 cells and may be considered as a potential anti-cancer agent for breast cancer treatments.

scholarly journals IGFBP5 induces cell adhesion, increases cell survival and inhibits cell migration in MCF-7 human breast cancer cells

2012 ◽  
Vol 125 (7) ◽  
pp. 1693-1705 ◽  
Author(s):  
A. Sureshbabu ◽  
H. Okajima ◽  
D. Yamanaka ◽  
E. Tonner ◽  
S. Shastri ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Cell Adhesion ◽  
Cell Survival ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7
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