Quality of Red Blood Cells Isolated from Umbilical Cord Blood Stored at Room Temperature

Red blood cells (RBCs) from cord blood contain fetal hemoglobin that is predominant in newborns and, therefore, may be more appropriate for neonatal transfusions than currently transfused adult RBCs. Post-collection, cord blood can be stored at room temperature for several days before it is processed for stem cells isolation, with little known about how these conditions affect currently discarded RBCs. The present study examined the effect of the duration cord blood spent at room temperature and other cord blood characteristics on cord RBC quality. RBCs were tested immediately after their isolation from cord blood using a broad panel of quality assays. No significant decrease in cord RBC quality was observed during the first 65 hours of storage at room temperature. The ratio of cord blood to anticoagulant was associated with RBC quality and needs to be optimized in future. This knowledge will assist in future development of cord RBC transfusion product.

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In VitroLarge Scale Production of Human Mature Red Blood Cells from Hematopoietic Stem Cells by Coculturing with Human Fetal Liver Stromal Cells

BioMed Research International ◽

10.1155/2013/807863 ◽

2013 ◽

Vol 2013 ◽

pp. 1-12 ◽

Cited By ~ 7

Author(s):

Jiafei Xi ◽

Yanhua Li ◽

Ruoyong Wang ◽

Yunfang Wang ◽

Xue Nan ◽

...

Keyword(s):

Stem Cells ◽

Hematopoietic Stem Cells ◽

Cord Blood ◽

Red Blood Cells ◽

Stromal Cells ◽

Blood Cells ◽

Fetal Liver ◽

Erythroid Differentiation ◽

Erythroid Cells ◽

Hematopoietic Stem

In vitromodels of human erythropoiesis are useful in studying the mechanisms of erythroid differentiation in normal and pathological conditions. Here we describe an erythroid liquid culture system starting from cord blood derived hematopoietic stem cells (HSCs). HSCs were cultured for more than 50 days in erythroid differentiation conditions and resulted in a more than 109-fold expansion within 50 days under optimal conditions. Homogeneous erythroid cells were characterized by cell morphology, flow cytometry, and hematopoietic colony assays. Furthermore, terminal erythroid maturation was improved by cosculturing with human fetal liver stromal cells. Cocultured erythroid cells underwent multiple maturation events, including decrease in size, increase in glycophorin A expression, and nuclear condensation. This process resulted in extrusion of the pycnotic nuclei in up to 80% of the cells. Importantly, they possessed the capacity to express the adult definitiveβ-globin chain upon further maturation. We also show that the oxygen equilibrium curves of the cord blood-differentiated red blood cells (RBCs) are comparable to normal RBCs. The large number and purity of erythroid cells and RBCs produced from cord blood make this method useful for fundamental research in erythroid development, and they also provide a basis for future production of available RBCs for transfusion.

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Therapeutic use of red blood cells and platelets derived from human cord blood stem cells

Stem Cells Translational Medicine ◽

10.1002/sctm.20-0517 ◽

2021 ◽

Vol 10 (S2) ◽

Author(s):

Xiaoyan Xie ◽

Hailei Yao ◽

Xiaoyan Han ◽

Wen Yue ◽

Xuetao Pei

Keyword(s):

Stem Cells ◽

Cord Blood ◽

Red Blood Cells ◽

Blood Cells ◽

Therapeutic Use ◽

Human Cord Blood ◽

Blood Stem Cells ◽

Cord Blood Stem Cells

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45. Quality parameters of red blood cells isolated from room temperature-stored umbilical cord blood

Cryobiology ◽

10.1016/j.cryobiol.2011.09.048 ◽

2011 ◽

Vol 63 (3) ◽

pp. 318

Author(s):

Mariia Zhurova ◽

John Akabutu ◽

Jason Acker

Keyword(s):

Cord Blood ◽

Red Blood Cells ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Blood Cells ◽

Room Temperature ◽

Quality Parameters

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AS-7 improved in vitro quality of red blood cells prepared from whole blood held overnight at room temperature

Transfusion ◽

10.1111/trf.12779 ◽

2014 ◽

Vol 55 (1) ◽

pp. 108-114 ◽

Cited By ~ 4

Author(s):

Margaret F. Veale ◽

Geraldine Healey ◽

Amrita Sran ◽

Katherine A. Payne ◽

Majid Zia ◽

...

Keyword(s):

Red Blood Cells ◽

Whole Blood ◽

Blood Cells ◽

Room Temperature

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Production of hematopoietic cells from umbilical cord blood stem cells for transfusion purposes: Focus on ex vivo generation of red blood cells

SCRIPTA MEDICA ◽

10.5937/scriptamed1202099i ◽

2012 ◽

Vol 43 (2) ◽

pp. 99-105

Author(s):

Zoran Ivanović ◽

Marija Vlaski

Keyword(s):

Stem Cells ◽

Cord Blood ◽

Red Blood Cells ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Blood Cells ◽

Ex Vivo ◽

Hematopoietic Cells ◽

Blood Stem Cells ◽

Cord Blood Stem Cells

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Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

International Journal of Molecular Sciences ◽

10.3390/ijms20194875 ◽

2019 ◽

Vol 20 (19) ◽

pp. 4875 ◽

Cited By ~ 1

Author(s):

Vanegas ◽

Galindo ◽

Páez-Gutiérrez ◽

González-Acero ◽

Medina-Valderrama ◽

...

Keyword(s):

Human Leukocyte Antigen ◽

Cell Viability ◽

Cord Blood ◽

Red Blood Cells ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Blood Cells ◽

Human Leukocyte ◽

Hla Typing ◽

Leukocyte Antigen

Hematopoietic progenitor cell (HPC) transplantation is a treatment option for malignant and nonmalignant diseases. Umbilical cord blood (UCB) is an important HPC source, mainly for pediatric patients. It has been demonstrated that human leukocyte antigen (HLA) matching and cell dose are the most important features impacting clinical outcomes. However, UCB matching is performed using low resolution HLA typing and it has been demonstrated that the unnoticed mismatches negatively impact the transplant. Since we found differences in CD34+ viability after thawing of UCB units matched for two different patients (p = 0.05), we presumed a possible association between CD34+ cell viability and HLA. We performed a multivariate linear model (n = 67), comprising pre-cryopreservation variables and high resolution HLA genotypes separately. We found that pre-cryopreservation red blood cells (RBC), granulocytes, and viable CD34+ cell count significantly impacted CD34+ viability after thawing, along with HLA-B or -C (R2 = 0.95, p = 0.01; R2 = 0.56, p = 0.007, respectively). Although HLA-B*40:02 may have a negative impact on CD34+ cell viability, RBC depletion significantly improves it.

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A comparison of murine T-cell–depleted adult bone marrow and full-term fetal blood cells in hematopoietic engraftment and immune reconstitution

Blood ◽

10.1182/blood.v99.1.364 ◽

2002 ◽

Vol 99 (1) ◽

pp. 364-371 ◽

Cited By ~ 16

Author(s):

Benny J. Chen ◽

Xiuyu Cui ◽

Gregory D. Sempowski ◽

Maria E. Gooding ◽

Congxiao Liu ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

T Cells ◽

T Cell ◽

Cord Blood ◽

Blood Cells ◽

Immune Reconstitution ◽

Full Term ◽

Fetal Blood ◽

Adult Bone

Umbilical cord blood has been increasingly used as a source of hematopoietic stem cells. A major area of concern for the use of cord blood transplantation is the delay in myeloid and lymphoid recovery. To directly compare myeloid and lymphoid recovery using an animal model of bone marrow and cord blood as sources of stem cells, hematopoietic engraftment and immune recovery were studied following infusion of T-cell–depleted adult bone marrow or full-term fetal blood cells, as a model of cord blood in a murine allogeneic transplantation model (C57BL/6 [H-2b] → BALB/c [H-2d]). Allogeneic full-term fetal blood has poorer radioprotective capacity but greater long-term engraftment potential on a cell-to-cell basis compared with T-cell–depleted bone marrow. Allogeneic full-term fetal blood recipients had decreased absolute numbers of T, B, and dendritic cells compared with bone marrow recipients. Splenic T cells in allogeneic full-term fetal blood recipients proliferated poorly, were unable to generate cytotoxic effectors against third-party alloantigens in vitro, and failed to generate alloantigen-specific cytotoxic antibodies in vivo. In addition, reconstituting T cells in fetal blood recipients had decreased mouse T-cell receptorδ single-joint excision circles compared with bone marrow recipients. At a per-cell level, B cells from fetal blood recipients did not proliferate as well as those found in bone marrow recipients. These results suggest that full-term fetal blood can engraft allogeneic hosts across the major histocompatibility barrier with slower hematopoietic engraftment and impaired immune reconstitution.

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