scholarly journals Cultivable Anaerobic Microbiota of Infected Root Canals

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Takuichi Sato ◽  
Keiko Yamaki ◽  
Naoko Ishida ◽  
Kazuhiro Hashimoto ◽  
Yasuhisa Takeuchi ◽  
...  
Keyword(s):  
Anaerobic Bacteria ◽  
16S Rrna Gene Sequencing ◽  
Bacterial Count ◽  
Oral Bacteria ◽  
Rrna Gene ◽  
Anaerobic Culture ◽  
Root Canals ◽  
Infected Root ◽  
Rrna Gene Sequencing ◽  
Molecular Biological Techniques

Objective. Periapical periodontitis is an infectious and inflammatory disease of the periapical tissues caused by oral bacteria invading the root canal. In the present study, profiling of the microbiota in infected root canals was performed using anaerobic culture and molecular biological techniques for bacterial identification.Methods. Informed consent was obtained from all subjects (age ranges, 34–71 years). Nine infected root canals with periapical lesions from 7 subjects were included. Samples from infected root canals were collected, followed by anaerobic culture on CDC blood agar plates. After 7 days, colony forming units (CFU) were counted and isolated bacteria were identified by 16S rRNA gene sequencing.Results. The mean bacterial count (CFU) in root canals was(0.5±1.1)×106(range8.0×101–3.1×106), and anaerobic bacteria were predominant (89.8%). The predominant isolates wereOlsenella(25.4%),Mogibacterium(17.7%),Pseudoramibacter(17.7%),Propionibacterium(11.9%) andParvimonas(5.9%).Conclusion. The combination of anaerobic culture and molecular biological techniques makes it possible to analyze rapidly the microbiota in infected root canals. The overwhelming majority of the isolates from infected root canals were found to be anaerobic bacteria, suggesting that the environment in root canals is anaerobic and therefore support the growth of anaerobes.

Cultivable bacteria in infected root canals as identified by 16S rRNA gene sequencing

2007 ◽  
Vol 22 (4) ◽  
pp. 266-271 ◽  
Author(s):  
J. F. Siqueira ◽  
I. N. Rôças ◽  
S. S. M. Paiva ◽  
K. M. Magalhães ◽  
T. Guimarães-Pinto
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Root Canals ◽  
Cultivable Bacteria ◽  
Infected Root ◽  
Rrna Gene Sequencing

scholarly journals Characterization of Actinomyces Isolates from Infected Root Canals of Teeth: Description of Actinomyces radicidentis sp. nov.

2000 ◽  
Vol 38 (9) ◽  
pp. 3399-3403 ◽  
Author(s):  
Matthew D. Collins ◽  
Lesley Hoyles ◽  
Sotos Kalfas ◽  
Goran Sundquist ◽  
Tor Monsen ◽  
...  
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Root Canals ◽  
Infected Root ◽  
Sequencing Studies ◽  
Rrna Gene Sequencing ◽  
Biochemical Testing

Two strains of a previously undescribedActinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. Analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct from previously described Actinomyces andArcanobacterium species. Comparative 16S rRNA gene-sequencing studies showed the bacterium to be a hitherto unknown subline within a group of Actinomyces species which includes Actinomyces bovis, the type species of the genus. Based on phylogenetic and phenotypic evidence, we propose that the unknown bacterium isolated from human clinical specimens be classified as Actinomyces radicidentis sp. nov. The type strain ofActinomyces radicidentis is CCUG 36733.

scholarly journals Comparison of the Vitek 2, API 20A, and 16s rRNA Gene Sequencing for the Identification of Anaerobic Bacteria

2015 ◽  
Vol 18 (1) ◽  
pp. 20 ◽  
Author(s):  
Gyun Cheol Park ◽  
Sook Jin Jang ◽  
Min Jung Lee ◽  
Joong-Ki Kook ◽  
Min Jung Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Anaerobic Bacteria ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Vitek 2 ◽  
Rrna Gene Sequencing

scholarly journals Contributions to human breast milk microbiome and enteromammary transfer of Bifidobacterium breve

2019 ◽  
Author(s):  
Kattayoun Kordy ◽  
Thaidra Gaufin ◽  
Martin Mwangi ◽  
Fan Li ◽  
Chiara Cerini ◽  
...  
Keyword(s):  
Breast Milk ◽  
Anaerobic Bacteria ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Human Breast Milk ◽  
Shotgun Metagenomics ◽  
Breastfed Infants ◽  
Skin Contact ◽  
Rrna Gene Sequencing

AbstractIncreasing evidence supports the importance of the breast milk microbiome in seeding the infant gut. However, the origin of bacteria in milk and the process of milk microbe-mediated seeding of infant intestine need further elucidation. Presumed sources of bacteria in milk include locations of mother-infant and mother-environment interactions. We investigate the role of mother-infant interaction on breast milk microbes. Shotgun metagenomics and 16S rRNA gene sequencing identified milk microbes of mother-infant pairs in breastfed infants and in infants that have never latched. Although breast milk has low overall biomass, milk microbes play an important role in seeding the infant gut. Breast milk bacteria were largely comprised of Staphylococcus, Streptococcus, Acinetobacter, and Enterobacter primarily derived from maternal areolar skin and infant oral sites in breastfeeding pairs. This suggests that the process of breastfeeding is a potentially important mechanism for propagation of breast milk microbes through retrograde flux via infant oral and areolar skin contact. In one infant delivered via Caesarian section, a distinct strain of Bifidobacteria breve was identified in maternal rectum, breast milk and the infant’s stool potentially suggesting direct transmission. This may support the existence of microbial translocation of this anaerobic bacteria via the enteromammary pathway in humans, where maternal bacteria translocate across the maternal gut and are transferred to the mammary glands. Modulating sources of human milk microbiome seeding potentially imply opportunities to ultimately influence the development of the infant microbiome and health.

scholarly journals 16S rRNA Gene Sequencing in Routine Identification of Anaerobic Bacteria Isolated from Blood Cultures: TABLE 1.

2010 ◽  
Vol 48 (3) ◽  
pp. 946-948 ◽  
Author(s):  
Ulrik Stenz Justesen ◽  
Marianne Nielsine Skov ◽  
Elisa Knudsen ◽  
Hanne Marie Holt ◽  
Per Søgaard ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Anaerobic Bacteria ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Blood Cultures ◽  
Rrna Gene ◽  
Routine Identification ◽  
Rrna Gene Sequencing

scholarly journals Temporal Shifts in Microbial Communities in Nonpregnant African-American Women with and without Bacterial Vaginosis

2008 ◽  
Vol 2008 ◽  
pp. 1-9 ◽  
Author(s):  
John Wertz ◽  
Natasha Isaacs-Cosgrove ◽  
Claudia Holzman ◽  
Terence L. Marsh
Keyword(s):  
Bacterial Vaginosis ◽  
African American Women ◽  
Anaerobic Bacteria ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Sexually Transmitted ◽  
Community Shift ◽  
Vaginal Tract ◽  
Phylogenetic Composition ◽  
Rrna Gene Sequencing

Bacterial vaginosis (BV) has been described as an increase in the number of anaerobic and facultatively anaerobic bacteria relative to lactobacilli in the vaginal tract. Several undesirable consequences of this community shift can include irritation, white discharge, an elevated pH, and increased susceptibility to sexually transmitted infections. While the etiology of the condition remains ill defined, BV has been associated with adverse reproductive and pregnancy outcomes. In order to describe the structure of vaginal communities over time we determined the phylogenetic composition of vaginal communities from seven women sampled at multiple points using 16S rRNA gene sequencing. We found that women with no evidence of BV had communities dominated by lactobacilli that appeared stable over our sampling periods while those with BV had greater diversity and decreased stability overtime. In addition, onlyLactobacillus inerswas found in BV positive communities.

Screening and Molecular Characterization of Cellulase Producing Actinobacteria from Litchi Orchard

2019 ◽  
Vol 13 (1) ◽  
pp. 90-101
Author(s):  
Sanju Kumari ◽  
Utkarshini Sharma ◽  
Rohit Krishna ◽  
Kanak Sinha ◽  
Santosh Kumar
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Biochemical Characterization ◽  
Evolutionary Relationship ◽  
Gene Sequencing ◽  
Cellulase Activity ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Rrna Gene Sequencing

Background: Cellulolysis is of considerable economic importance in laundry detergents, textile and pulp and paper industries and in fermentation of biomass into biofuels. Objective: The aim was to screen cellulase producing actinobacteria from the fruit orchard because of its requirement in several chemical reactions. Methods: Strains of actinobacteria were isolated on Sabouraud’s agar medium. Similarities in cultural and biochemical characterization by growing the strains on ISP medium and dissimilarities among them perpetuated to recognise nine groups of actinobacteria. Cellulase activity was measured by the diameter of clear zone around colonies on CMC agar and the amount of reducing sugar liberated from carboxymethyl cellulose in the supernatant of the CMC broth. Further, 16S rRNA gene sequencing and molecular characterization were placed before NCBI for obtaining recognition with accession numbers. Results: Prominent clear zones on spraying Congo Red were found around the cultures of strains of three groups SK703, SK706, SK708 on CMC agar plates. The enzyme assay for carboxymethylcellulase displayed extra cellulase activity in broth: 0.14, 0.82 and 0.66 µmol mL-1 min-1, respectively at optimum conditions of 35°C, pH 7.3 and 96 h of incubation. However, the specific cellulase activities per 1 mg of protein did not differ that way. It was 1.55, 1.71 and 1.83 μmol mL-1 min-1. The growing mycelia possessed short compact chains of 10-20 conidia on aerial branches. These morphological and biochemical characteristics, followed by their verification by Bergey’s Manual, categorically allowed the strains to be placed under actinobacteria. Further, 16S rRNA gene sequencing, molecular characterization and their evolutionary relationship through phylogenetics also confirmed the putative cellulase producing isolates of SK706 and SK708 subgroups to be the strains of Streptomyces. These strains on getting NCBI recognition were christened as Streptomyces glaucescens strain SK91L (KF527284) and Streptomyces rochei strain SK78L (KF515951), respectively. Conclusion: Conclusive evidence on the basis of different parameters established the presence of cellulase producing actinobacteria in the litchi orchard which can convert cellulose into fermentable sugar.

scholarly journals Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white Pieris canidia (Lepidoptera: Pieridae)

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 254
Author(s):  
Ying Wang ◽  
Jianqing Zhu ◽  
Jie Fang ◽  
Li Shen ◽  
Shuojia Ma ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
16S Rrna Gene Sequencing ◽  
Adult Survival ◽  
Rrna Gene ◽  
Life Stages ◽  
Microbial Composition ◽  
Significant Difference ◽  
Functional Inference ◽  
Rrna Gene Sequencing ◽  
Insect Fitness

We characterized the gut microbial composition and relative abundance of gut bacteria in the larvae and adults of Pieris canidia by 16S rRNA gene sequencing. The gut microbiota structure was similar across the life stages and sexes. The comparative functional analysis on P. canidia bacterial communities with PICRUSt showed the enrichment of several pathways including those for energy metabolism, immune system, digestive system, xenobiotics biodegradation, transport, cell growth and death. The parameters often used as a proxy of insect fitness (development time, pupation rate, emergence rate, adult survival rate and weight of 5th instars larvae) showed a significant difference between treatment group and untreated group and point to potential fitness advantages with the gut microbiomes in P. canidia. These data provide an overall view of the bacterial community across the life stages and sexes in P. canidia.

Sign in / Sign up

Export Citation Format

Share Document