Induction of Mitochondria-Mediated Apoptosis in Ca Ski Human Cervical Cancer Cells Triggered by Mollic Acid Arabinoside Isolated fromLeea indica

Leea indicais a medicinal plant traditionally used to treat cancer. Through bioassay-guided approach, we isolated mollic acid arabinoside (MAA), for the first time fromLeea indica. Here, we present the apoptosis-inducing effect of MAA on Ca Ski cervical cancer cells. Based on DAPI staining, MAA-treated cells manifested nuclear shrinkage, condensation, and fragmentation. We further confirmed the fragmentation of DNA using TUNEL assay. During early apoptosis, MAA caused the perturbation of plasma membrane through externalization of PS, followed by the formation of apoptotic blebs. Prior to these events, MAA triggered rapid dissipation of the mitochondrial membrane potential. In the upstream, MAA increased the expression of Bax, decreased the expression of Bcl-2, and augmented the Bax/Bcl-2 ratio. These findings suggested that MAA induced mitochondrial-mediated apoptosis in Ca Ski cells and thus provide the scientific explanation for the traditional application of this herbal medicine in cancer treatment.

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Pinelliae Rhizoma Herbal-Acupuncture Solution Induced Apoptosis in Human Cervical Cancer Cells, SNU-17

The American Journal of Chinese Medicine ◽

10.1142/s0192415x0600393x ◽

2006 ◽

Vol 34 (03) ◽

pp. 401-408 ◽

Cited By ~ 11

Author(s):

Jong-Seok Yoon ◽

Jung-Chul Seo ◽

Sang-Won Han

Keyword(s):

Cervical Cancer ◽

Cell Death ◽

Cancer Cells ◽

Caspase 3 ◽

Dapi Staining ◽

Apoptotic Gene ◽

Cervical Cancer Cells ◽

Induced Apoptosis ◽

Human Cervical Cancer Cells ◽

Human Cervical Cancer

Pinelliae Rhizoma has been used traditionally in Korea to promote the liver Qi activity and the function of the digestive system. We investigated whether the Pinelliae Rhizoma herbal-acupuncture solution (PRHS) would induce cell-death on SNU-17, human cervical cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to investigate the cytotoxicity of PRHS. The cell death was identified as apoptosis with 4, 6-diamidineo-2-phenylindole (DAPI) staining, and terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. PRHS could induce apoptosis of SNU-17 via Bax-related caspase-3 activation. The expressions of both Bax, a pro-apoptotic gene, and caspase-3, an apoptotic gene, were increased. The results might provide the experimental data for the clinical use of Pinelliae Rhizoma on cervical cancer.

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Antiproliferative Effects of Palmitoylethanolamide on Human Cervical Cancer Cells

10.28991/iccr-2019-020 ◽

2019 ◽

Author(s):

Laura Bonfili ◽

Valentina Cecarini ◽

Anna Maria Eleuteri

Keyword(s):

Cervical Cancer ◽

Cancer Cells ◽

Antiproliferative Effects ◽

Cervical Cancer Cells ◽

Human Cervical Cancer Cells ◽

Human Cervical Cancer

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Genistein Induces Alterations of Epigenetic Modulatory Signatures in Human Cervical Cancer Cells

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520617666170918142114 ◽

2018 ◽

Vol 18 (3) ◽

pp. 412-421 ◽

Cited By ~ 24

Author(s):

Madhumitha Kedhari Sundaram ◽

Mohammad Zeeshan Ansari ◽

Abdullah Al Mutery ◽

Maryam Ashraf ◽

Reem Nasab ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Cells ◽

In Silico ◽

Tumour Suppressor Genes ◽

Dietary Polyphenols ◽

In Silico Studies ◽

Genistein Treatment ◽

Cervical Cancer Cells ◽

Human Cervical Cancer Cells ◽

Human Cervical Cancer

Introduction: Epidemiological studies indicate that diet rich in fruits and vegetables is associated with decreased cancer risk thereby indicating that dietary polyphenols can be potential chemo-preventive agents. The reversible nature of epigenetic modifications makes them a favorable target for cancer prevention. Polyphenols have been shown to reverse aberrant epigenetic patterns by targeting the regulatory enzymes, DNA methyltransferases (DNMTs) and histone deacetylases (HDACs). In vitro and in silico studies of DNMTs and HDACs were planned to examine genistein’s role as a natural epigenetic modifier in human cervical cancer cells, HeLa. Methods: Expression of the tumour suppressor genes (TSGs) [MGMT, RARβ, p21, E-cadherin, DAPK1] as well the methylation status of their promoters were examined alongwith the activity levels of DNMT and HDAC enzymes after treatment with genistein. Expression of DNMTs and HDACs was also studied. In-silico studies were performed to determine the interaction of genistein with DNMTs and HDACs. Results: Genistein treatment significantly reduced the expression and enzymatic activity of both DNMTs and HDACs in a time-dependent way. Molecular modeling data suggest that genistein can interact with various members of DNMT and HDAC families and support genistein mediated inhibition of their activity. Timedependent exposure of genistein reversed the promoter region methylation of the TSGs and re-established their expression. Conclusions: In this study, we find that genistein is able to reinstate the expression of the TSGs studied by inhibiting the action of DNMTs and HDACs. This shows that genistein could be an important arsenal in the development of epigenetic based cancer therapy.

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