scholarly journals Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Gonca Cayir Keles ◽  
Umut Balli ◽  
Burcu Ozkan Cetinkaya ◽  
Bulent Ayas ◽  
Arzu Findik ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Acute Phase Proteins ◽  
Plasma Fibrinogen ◽  
Gingival Tissue ◽  
Pentraxin 3 ◽  
Tissue Destruction ◽  
Serum Samples ◽  
Periodontal Inflammation ◽  
Significant Difference ◽  
Experimental Periodontitis

Objective.Pentraxin 3 (PTX3), newly discovered inflammation marker, is a member of acute-phase proteins. The hypothesis, synthesis of gingival tissue and serum PTX-3 increases in the experimental periodontitis model (with 10-day and 40-day periods), was tested by detecting gingival tissue and serum PTX-3 levels in rats with experimental periodontitis.Methods.Thirty rats were randomly divided into three groups of ten animals each: ligature-induced experimental periodontitis groups (with 10-day (Group1) and 40-day periods (Group2)) and healthy group (Group3). At the end of experimental period, rats were sacrificed, and radiological and histomorphometric analyses were performed on the mandibles. PTX3 levels were measured in gingival tissue and serum samples using ELISA. Plasma fibrinogen levels were measured according to the nephelometric method.Results.Significant alveolar bone resorption and periodontal inflammation were evident in periodontitis groups. Levels of PTX3 in gingival tissue were statistically higher in Group 1 than those in groups 2 and 3 (P<0.01). No significant difference was found in serum PTX3 levels between experimental periodontitis and control groups (P>0.05). Plasma fibrinogen levels were significantly increased in the experimental periodontitis groups (P<0.001).Conclusion.PTX3 seems to be associated with tissue destruction in earlier periods of inflammatory periodontal disease, contrary to the fibrinogen findings.

scholarly journals Caffeic Acid Phenethyl Ester Attenuates Gingival Tissue Inflammation in Experimental Periodontitis

2021 ◽  
Author(s):  
Esra DEMİR ◽  
Feyza Otan ÖZDEN ◽  
Bahattin AVCI
Keyword(s):  
Caffeic Acid ◽  
Alveolar Bone ◽  
Caffeic Acid Phenethyl Ester ◽  
Gingival Tissue ◽  
Dependent Manner ◽  
Tnf Α ◽  
Significant Difference ◽  
Experimental Periodontitis ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract Caffeic acid phenethyl ester (CAPE) is an active component of propolis extracts and has anti-inflammatory, antioxidant, immunomodulatory activities. This study aims to investigate the anti-inflammatory effect of two different dosages of CAPE on lipopolysaccharide-induced experimental periodontitis (EP). Forty Sprague Dawley rats were randomly divided into four groups: control, EP, EP treated with 5 µmol/kg/day of CAPE (EP + CAPE 5), and EP treated with 10 µmol/kg/day of CAPE (EP + CAPE 10). Followed by the EP, CAPE was administered intraperitoneally to the EP + CAPE groups for 28 days. Samples were investigated biochemically using an enzyme linked immunoassay (ELISA) kit and alveolar bone loss was measured morphometrically. In both of the CAPE groups, the levels of IL-1β and TNF-α in the gingiva were significantly lower than those in the EP group (p < 0.001). The decrease in tissue levels of TNF-α was greater in the EP + CAPE 10 group than in the EP + CAPE 5 group in a dose-dependent manner. Serum analysis of the cytokines showed no significant difference between the groups. Within the limits of this study, CAPE showed its anti-inflammatory effect by reducing pro-inflammatory cytokines in gingiva and is claimed to be a novel agent in improving the results of periodontal therapy without any known side effects.

Effect of Paeonol on Tissue Destruction in Experimental Periodontitis of Rats

2014 ◽  
Vol 42 (02) ◽  
pp. 361-374 ◽  
Author(s):  
Chih-Yuan Chang ◽  
Earl Fu ◽  
Cheng-Yang Chiang ◽  
Wei-Jeng Chang ◽  
Wan-Chien Cheng ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Inflammatory Cytokines ◽  
Alveolar Bone ◽  
Gingival Tissue ◽  
Tartrate Resistant Acid Phosphatase ◽  
Micro Ct ◽  
Tissue Destruction ◽  
Tissue Inflammation ◽  
Experimental Periodontitis ◽  
Pro Inflammatory Cytokines

We evaluated the effects of paeonol, a phenolic compound of Moutan Cortex, on the tissue inflammation and destruction in experimental periodontitis of rats. The maxillary palatal bony surfaces of 18 rats received injections of lipopolysaccharide (LPS, 5 mg/mL), PBS or LPS-plus-paeonol (40 mg/kg, intra-peritoneal injection) for three days. Five days later, the osteoclasts were examined and compared after tartrate-resistant acid phosphatase staining. In another 36 rats, the experimental periodontitis was induced by placing the ligatures around the maxillary second and mandibular first molars. Seven days later, the periodontal destruction and inflammation in rats with paeonol (40 mg/kg or 80 mg/kg) and those who had no ligature or without paeonol were compared by dental radiography, micro-computerized tomography (micro-CT), and histology. Gingival mRNA expressions of pre-inflammatory cytokines, including IL-1β' IL-6 and TNF-α were also examined. Compared to the effect of the LPS positive control, the paeonol injection significantly reduced the induced osteoclast formation. In ligature-induced periodontitis, the periodontal bone supporting ratio was significantly higher in the ligature-plus-paeonol groups compared to that of the ligature group, although they were still less than those in the non-ligature group. By micro-CT and by histology/histometry, a consistent anti-destructive effect was observed when paeonol was added. Moreover, less amount of inflammatory cell-infiltrated connective tissue area, connective tissue attachment, and mRNA expressions of pro-inflammatory cytokines were presented in the ligature-plus-paeonol groups than those in the ligature group. These results suggested that paeonol might have a protective potential on gingival tissue inflammation and alveolar bone loss during the process of periodontitis by inhibiting pro-inflammatory cytokines.

scholarly journals Antiperiodontitis Effects of Magnolia biondii Extract on Ligature-Induced Periodontitis in Rats

Nutrients ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 934 ◽  
Author(s):  
Hae Jin Lee ◽  
Dong-Ryung Lee ◽  
Bong-Keun Choi ◽  
Seung Hwan Yang
Keyword(s):  
Bone Loss ◽  
Tissue Damage ◽  
Alveolar Bone ◽  
Histopathological Examination ◽  
Nuclear Factor Κb ◽  
Alveolar Bone Loss ◽  
Gingival Tissue ◽  
Protective Effects ◽  
Tissue Destruction ◽  
Commercial Kits

Over the past decades, periodontitis has become a rising health problem and caused various diseases. In the many studies shows that some extracts and compound to the prevention and treatment of periodontitis. This study focuses on the effects of inhibition of gingival damage and alveolar bone loss. The aim of this study was to evaluate the protective effects of Magnolia biondii extract (MBE) against ligature-induced periodontitis in rats. A ligature was placed around the molar teeth for 8 weeks, and MBE was administered for 8 weeks. Gingival tissue damage and alveolar bone loss were measured by microcomputed tomography (CT) analysis and histopathological examination. Serum Interluekin-1 β (IL-1β), tumor necrosis factor-α (TNF-α), cyclooxygenases-2 (COX-2), and receptor activator of nuclear factor–κB ligand (RANKL) levels were investigated using commercial kits to confirm the antiperiodontitis effects of MBE. We confirmed that ligature-induced periodontitis resulted in gingival tissue damage and alveolar bone loss. However, treatment for 8 weeks with MBE protected from periodontal tissue damage and downregulated serum inflammatory cytokine factors and RANKL levels. These results suggest that MBE exerts antiperiodontitis effects by inhibiting gingival tissue destruction and alveolar bone loss through regulation of anti-inflammatory cytokines in periodontitis-induced rats.

scholarly journals Effects of Gelam Honey (Melaleuca cajuputi) on Alveolar Bone Loss in Experimental Periodontitis

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
N. Hamzah ◽  
S. A. Aziz ◽  
A. R. Fauzi ◽  
Y. A. Mohd Yusof ◽  
M. Razali ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Melaleuca Cajuputi ◽  
Significant Difference ◽  
Gelam Honey ◽  
Bone Level ◽  
Experimental Periodontitis

Gelam honey has been shown to exhibit antioxidant and anti-inflammatory activities in animal model. The aim of this study was to determine the effects of Gelam honey (Melaleuca cajuputi) on alveolar bone level in experimental periodontitis. Thirty male Sprague-Dawley rats were used in this study and randomly divided into four groups: ligated saline (LS), ligated honey (LH), nonligated saline (NLS), and nonligated honey (NLH). Fifteen days after supplementation with Gelam honey (3 g/kg), the rats were sacrificed and alveolar bone level was determined by radiography and histomorphometry. The number of osteoclasts was also calculated for all groups. Both radiographic and histomorphometric analyses showed that alveolar bone resorption was severely induced around the ligated molar in the LS and LH groups. There was no significant difference in alveolar bone level between the LS and LH groups. However, there was a nonsignificant reduction of osteoclast number by 15.2% in LH group compared to LS group. In the NLH group, there was less alveolar bone resorption and the number of osteoclasts was reduced by 13.2% compared to NLS group. In conclusion, systemically supplemented Gelam honey was shown to have the potential of reducing osteoclast activity in the experimental periodontitis rats, even though the effect on alveolar bone level was not well demonstrated and it warrants further research.

Rosiglitazone Reduces the Evolution of Experimental Periodontitis in the Rat

2006 ◽  
Vol 85 (2) ◽  
pp. 156-161 ◽  
Author(s):  
R. Di Paola ◽  
E. Mazzon ◽  
D. Maiere ◽  
D. Zito ◽  
D. Britti ◽  
...  
Keyword(s):  
Cellular Proliferation ◽  
Alveolar Bone ◽  
Bone Destruction ◽  
Neutrophil Infiltration ◽  
Tissue Expression ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Ppar Γ ◽  
Periodontal Inflammation ◽  
Experimental Periodontitis

The peroxisome proliferator-activated receptor-γ (PPAR-γ) receptor appears to play a pivotal role in the regulation of cellular proliferation and inflammation. Recent evidence also suggests that rosiglitazone, a PPAR-γ agonist, reduces acute and chronic inflammation. We hypothesized that rosiglitazone would attenuate periodontal inflammation. In the present study, we investigated the effects of rosiglitazone in a rat model of ligature-induced periodontitis. At day 8, ligation significantly induced an increase in neutrophil infiltration, as well as of gingivomucosal tissue expression of iNOS, nitrotyrosine formation, and poly (ADP-ribose) polymerase activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone destruction. Intraperitoneal injection of rosiglitazone (10 mg/kg 10% DMSO daily for 8 days) significantly decreased all of the parameters of inflammation, as described above. Analysis of these data demonstrated that rosiglitazone exerted an anti-inflammatory role during experimental periodontitis, and was able to ameliorate the tissue damage associated with ligature-induced periodontitis.

scholarly journals Evaluation of Gene Polymorphism and Gingival Crevicular Fluid Levels of Matrix Metalloproteinase-3 in a Group of Turkish Periodontitis Patients

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1260
Author(s):  
Gökhan Kasnak ◽  
Mustafa Yılmaz ◽  
Revan Birke Koca Ünsal ◽  
Nuray Gürel Polat ◽  
Erhan Fıratlı
Keyword(s):  
Gingival Crevicular Fluid ◽  
Venous Blood ◽  
Turkish Population ◽  
Stage Ii ◽  
Enzyme Linked Immunosorbent Assay ◽  
Collagen Structure ◽  
Tissue Destruction ◽  
Periodontal Inflammation ◽  
Significant Difference ◽  
Crevicular Fluid

Introduction: Periodontitis is characterized by the destruction of tooth-supporting tissues. Matrix metalloproteinases (MMPs) play a significant part in the degradation of collagen structure. The gingival crevicular fluid (GCF) levels of MMPs increase with the progression of periodontal inflammation. Polymorphisms can be responsible for high expression of MMPs and can exacerbate the breakdown of collagen structure. This study aims to investigate the effect of MMP-3 -1171 5A/6A polymorphism and the GCF levels of MMP-3 in a group of Turkish periodontitis patients. Materials and Methods: Non-smoking, stage II grade A periodontitis (S II-Gr A) (n = 68) and stage II grade B periodontitis (S II-Gr C) (n = 64) patients were recruited. Healthy individuals (H) (n = 72) without signs of gingivitis or periodontitis served as the control. Venous blood was collected from participants to obtain DNA, and the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was used to detect polymorphism. GCF samples were taken to assess MMP-3 levels using an enzyme-linked immunosorbent assay (ELISA). Results: The MMP-3 -1179 5A/6A distribution showed no significant difference between the groups (p > 0.05). However, the MMP-3 GCF levels of the S II-Gr C group were higher than those of both the S II-Gr A and H groups (p < 0.05), and elevated MMP-3 levels were detected in S II-Gr A compared to H (p < 0.05). Conclusion: The MMP-3 GCF levels showed an association with periodontal tissue destruction, although single nucleotide polymorphism was not associated with the S II-Gr C and S II-Gr A groups in the Turkish population.

Periodontitis-mediated Conversion of CD4+CD25+ Regulatory T Cells into Proinflammatory Interleukin 17-producing T Helper Cells

2021 ◽  
Author(s):  
Hongrui Liu ◽  
Dongfang Li ◽  
Minqi Li
Keyword(s):  
Th17 Cell ◽  
Alveolar Bone ◽  
Treg Cells ◽  
Inflammatory Factors ◽  
Gingival Tissue ◽  
Control Group ◽  
Interleukin 17 ◽  
Related Gene ◽  
T Helper ◽  
Experimental Periodontitis

Abstract Background: Among CD4+ T helper (Th) cells, Th17-Treg imbalance is a major pathogenesis of Chronic periodontitis (CP). Treg cells are often considered to play a protective role but they may have plasticity. This study aimed to clarify the regulatory role of Th17-Treg balance in periodontitis and further reveal Treg plasticity.Methods: An experimental periodontitis model was established by ligation of a silk thread and local injection of Pg-LPS. Inflammatory factors in serum and gingival tissues were measured by ELISA and RT-PCR. The degree of alveolar bone absorption was evaluated by micro-CT and histomorphological analysis. Quantities of Treg and Th17 cell and their related gene expression levels were examined. Furthermore, after magnetic bead-sorting spleen Treg cells, their characteristic genes and Th17 cell-related factors were explored at the mRNA level.Results: Inflammatory cytokines in serum and gingival tissue increased significantly in experimental periodontitis, which revealed obvious crestal bone loss around maxillary second molars. Consistently, we found increased secretion of RANKL by osteoblasts, thereby promoting the formation of osteoclasts and enhancing their activity in periodontitis. Further analysis showed that the number of Th17 cells and expression of related genes increased more significantly than Treg cells, demonstrating Treg-Th17 imbalance in periodontitis. Flow cytometry showed that the proportions of Treg cells in the blood and spleen of the periodontitis group was lower than those of the control group. Furthermore, Treg cell-related gene Foxp3 was downregulated and their expression of Th17 cell-related genes Rorc and IL-17A were increased. Conclusions: These results provided evidence that periodontitis may lead to Treg-Th17 conversion, although its mechanisms requires further study.

Cinnamic acid decreases periodontal inflammation and alveolar bone loss in experimental periodontitis

2020 ◽  
Vol 55 (5) ◽  
pp. 676-685
Author(s):  
Ozkan Karatas ◽  
Hatice Balci Yuce ◽  
Mehmet Murat Taskan ◽  
Fikret Gevrek ◽  
Cemil Alkan ◽  
...  
Keyword(s):  
Bone Loss ◽  
Cinnamic Acid ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Periodontal Inflammation ◽  
Experimental Periodontitis

scholarly journals Clinical application of concentrate growth factors combined with bone substitute in Alveolar ridge preservation of anterior teeth

2021 ◽  
Author(s):  
Dilinuer Keranmu ◽  
Nijiati Nuermuhanmode ◽  
Ailimaierdan Ainiwaer ◽  
Adili Moming ◽  
Gu Li ◽  
...  
Keyword(s):  
Growth Factors ◽  
Alveolar Bone ◽  
Gingival Tissue ◽  
Control Group ◽  
Ridge Preservation ◽  
Alveolar Ridge ◽  
Anterior Teeth ◽  
Alveolar Ridge Preservation ◽  
Bone Powder ◽  
Significant Difference

Abstract Objective: To investigate the clinical effect of concentrated growth factors (CGF) combined with Bio-oss bone powder on Alveolar ridge preservation (ARP) during implantology. Methods: A total of 38 patients were selected and randomly divided into 2 groups, with 19 cases in each group. The extraction sockets were filled with Bio-oss bone powder with or without CGF. VAS pain score was recorded within1 week and Landry wound healing index (LWHI) was recorded at 1, 2 and 3 weeks after operation. CBCT was taken 3 and 6 months after operation to measure and compare the changes of vertical height, width and gray value of alveolar bone at extraction site. The changes of alveolar bone contour were observed clinically and compared between the two groups. Results: The VAS score of CGF group was lower than control group on the 1st and 3rd day after operation (P < 0.05). The LWHI of CGF group was higher than control group 1 week after operation (P < 0.05). The absorption of the labial and palatal plates height and the width in the CGF group was significantly less than the control group at 3 months (P<0.05). The gray value of alveolar bone in CGF group was significantly higher than control group at 3 months (P < 0.05). There was no significant difference in new bone contour between the two groups (P > 0.05). 94.7% cases in CGF group did not undergo bone grafting, which was significantly higher than control group (78.9%). Conclusions: The use of CGF combined with Bio-oss bone powder can help to reduce postoperative pain at the early stage of healing, form sufficient keratinized gingival tissue, effectively maintain the height and width of alveolar bone in the three-dimensional direction and provide good conditions for implant repair in the future.

Effects of Curcumin on Alveolar Bone Loss in Experimental Periodontitis in Rats: A Morphometric and Histopathologic Study

2017 ◽  
Vol 87 (5-6) ◽  
pp. 262-270 ◽  
Author(s):  
Aysun Akpinar¹ ◽  
Metin Calisir² ◽  
Nebi Cansın Karakan³ ◽  
Aysan Lektemur Alpan4 ◽  
Fahrettin Goze5 ◽  
...  
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Interleukin 10 ◽  
Alveolar Bone Loss ◽  
Control Group ◽  
Serum Samples ◽  
Male Wistar Rats ◽  
The Mean ◽  
Experimental Periodontitis ◽  
And Control

Abstract. Background: Curcumin is found in the rhizomes of the turmeric plant that has been showed antioxidant and anti-inflammatory effect. The aim of this study was to evaluate the effects of systemic curcumin therapy on alveolar bone loss in an experimental periodontitis model in rats. Material and Methods: Thirty-two male Wistar rats were randomly divided to 4 groups: 75 mg/kg/daily curcumin (C75; n =8), 150 mg/kg/daily curcumin (C150; n =8), Control (n =8), and Ligated (n =8). Curcumin was administrated using gastric gavage. After 12 days, the rats were sacrificed. Right mandibles samples were histopathologically examined. Alveolar bone loss was measured. Interleukin 1β (IL-1β) and interleukin 10 (IL-10) were evaluated in the serum samples and gingival homogenates. Results: The measurements of alveolar bone loss in the mandibular molars revealed significantly higher bone-loss values in the Ligated group than the Control, C75 and C150 groups. The IL-1β levels in the gingival homogenates were significantly increased in the Ligated group compared to those of the Control, C75 and C150 groups. The serum IL-1β levels in the Ligated group were significantly higher than the Control group. The mean osteoblast numbers in the Ligated group were lower than those of the Control, C75 and C150 groups. The C150 groups showed significantly more osteoblasts than the Control group. The osteoclast numbers in the Ligated group increased significantly compared to the C75, C150 and control groups. Conclusion: This study demonstrates that systemic administration of curcumin at the 75 and 150mg/kg doses reduced alveolar bone loss in the periodontal disease in rats. Keywords: Alveolar bone loss, Antioxidant, Curcumin, Ligature-induced, Histomorphometric, Micronutrition

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