Effect of Paeonol on Tissue Destruction in Experimental Periodontitis of Rats

2014 ◽  
Vol 42 (02) ◽  
pp. 361-374 ◽  
Author(s):  
Chih-Yuan Chang ◽  
Earl Fu ◽  
Cheng-Yang Chiang ◽  
Wei-Jeng Chang ◽  
Wan-Chien Cheng ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Inflammatory Cytokines ◽  
Alveolar Bone ◽  
Gingival Tissue ◽  
Tartrate Resistant Acid Phosphatase ◽  
Micro Ct ◽  
Tissue Destruction ◽  
Tissue Inflammation ◽  
Experimental Periodontitis ◽  
Pro Inflammatory Cytokines

We evaluated the effects of paeonol, a phenolic compound of Moutan Cortex, on the tissue inflammation and destruction in experimental periodontitis of rats. The maxillary palatal bony surfaces of 18 rats received injections of lipopolysaccharide (LPS, 5 mg/mL), PBS or LPS-plus-paeonol (40 mg/kg, intra-peritoneal injection) for three days. Five days later, the osteoclasts were examined and compared after tartrate-resistant acid phosphatase staining. In another 36 rats, the experimental periodontitis was induced by placing the ligatures around the maxillary second and mandibular first molars. Seven days later, the periodontal destruction and inflammation in rats with paeonol (40 mg/kg or 80 mg/kg) and those who had no ligature or without paeonol were compared by dental radiography, micro-computerized tomography (micro-CT), and histology. Gingival mRNA expressions of pre-inflammatory cytokines, including IL-1β' IL-6 and TNF-α were also examined. Compared to the effect of the LPS positive control, the paeonol injection significantly reduced the induced osteoclast formation. In ligature-induced periodontitis, the periodontal bone supporting ratio was significantly higher in the ligature-plus-paeonol groups compared to that of the ligature group, although they were still less than those in the non-ligature group. By micro-CT and by histology/histometry, a consistent anti-destructive effect was observed when paeonol was added. Moreover, less amount of inflammatory cell-infiltrated connective tissue area, connective tissue attachment, and mRNA expressions of pro-inflammatory cytokines were presented in the ligature-plus-paeonol groups than those in the ligature group. These results suggested that paeonol might have a protective potential on gingival tissue inflammation and alveolar bone loss during the process of periodontitis by inhibiting pro-inflammatory cytokines.

scholarly journals Effects of Gold Nanoparticles Combined Human β-defensin 3 on The Alveolar Bone of Experimental Periodontitis

2021 ◽  
Author(s):  
Jing Zhou ◽  
Lingjun Li ◽  
Di Cui ◽  
Xiaoting Xie ◽  
Wenrong Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Bone Resorption ◽  
Alveolar Bone ◽  
Ct Scanning ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tartrate Resistant Acid Phosphatase ◽  
Micro Ct ◽  
Tnf Α ◽  
Experimental Periodontitis ◽  
Masson Staining

Abstract Background Nanomaterials of biomedicine and tissue engineering have been proposed in the treatment of periodontitis recently. This study aimed to investigate the effect of gold nanoparticles (AuNPs) combined human β-defensin 3 (hBD3) on the repair of alveolar bone of experimental periodontitis in rats. Methods A model of experimental periodontitis was established by ligating of the maxillary second molars with silk thread in rats, which were treated with or without AuNPs combined hBD3. Micro-focus computerized tomography (micro-CT) scanning, enzyme-linked immunosorbent assay (ELISA) and histological and immunohistochemical staining, including alkaline phosphatase (ALP), osteoprotegerin (OPG) tartrate-resistant acid phosphatase (TRAP) and receptor activator of NF-κB Ligand (RANKL), were used to analyze. Results Micro-CT demonstrated that the alveolar bone resorption was significantly reduced after the treatment of AuNPs combined hBD3. Levels of TNF-α and IL-6 decreased markedly compared with the ligation group. HE and Masson staining showed that AuNPs combined hBD3 group had less inflammatory cell infiltration, collagen fibrosis and fracture, but higher calcification in the new bone tissue. Moreover, the administration of AuNPs combined hBD3 increased the expression of ALP and OPG (related to bone formation) expression, while decreased TRAP and RANKL (related to bone resorption) expression. Conclusions AuNPs combined hBD3 had a protective effect on the progress of experimental periodontitis in rats, and also played a certain role in promoting osteogenesis.

scholarly journals Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Jing Zhou ◽  
Lingjun Li ◽  
Di Cui ◽  
Xiaoting Xie ◽  
Wenrong Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Bone Resorption ◽  
Bone Repair ◽  
Alveolar Bone ◽  
Ct Scanning ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tartrate Resistant Acid Phosphatase ◽  
Micro Ct ◽  
Expression Levels ◽  
Experimental Periodontitis

Abstract Background Nanomaterials of biomedicine and tissue engineering have been proposed for the treatment of periodontitis in recent years. This study aimed to investigate the effects of gold nanoparticles (AuNPs) combined with human β-defensin 3 (hBD3) on the repair of the alveolar bones of experimental periodontitis in rats. Methods A model of experimental periodontitis was established by ligation of the maxillary second molars with silk thread in rats, which were treated with or without AuNPs combined with hBD3. Micro‐computerized tomography (micro-CT) scanning, enzyme-linked immunosorbent assay, and histological and immunohistochemical staining, including alkaline phosphatase (ALP), osteoprotegerin (OPG), tartrate-resistant acid phosphatase (TRAP), and receptor activator of NF-κB ligand (RANKL), were used to analyze the samples. Results Micro-CT demonstrated that the alveolar bone resorption was significantly reduced after the treatment with AuNPs combined with hBD3. Levels of TNF-α and IL-6 were decreased markedly compared with the ligation group. H&E and Masson staining showed that AuNPs combined with hBD3 group had less inflammatory cell infiltration, collagen fibrosis and fracture, but higher calcification in the new bone tissue. Moreover, the administration of AuNPs combined with hBD3 increased the expression levels of ALP and OPG (related to bone formation) while decreasing the expression levels of TRAP and RANKL (related to bone resorption) expression. Conclusions AuNPs combined with hBD3 had a protective effect on the progression of experimental periodontitis in rats and played a certain role in suppressing osteoclastogenesis and alleviating the inflammatory destruction of periodontitis along with the promotion of bone repair.

scholarly journals Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Gonca Cayir Keles ◽  
Umut Balli ◽  
Burcu Ozkan Cetinkaya ◽  
Bulent Ayas ◽  
Arzu Findik ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Acute Phase Proteins ◽  
Plasma Fibrinogen ◽  
Gingival Tissue ◽  
Pentraxin 3 ◽  
Tissue Destruction ◽  
Serum Samples ◽  
Periodontal Inflammation ◽  
Significant Difference ◽  
Experimental Periodontitis

Objective.Pentraxin 3 (PTX3), newly discovered inflammation marker, is a member of acute-phase proteins. The hypothesis, synthesis of gingival tissue and serum PTX-3 increases in the experimental periodontitis model (with 10-day and 40-day periods), was tested by detecting gingival tissue and serum PTX-3 levels in rats with experimental periodontitis.Methods.Thirty rats were randomly divided into three groups of ten animals each: ligature-induced experimental periodontitis groups (with 10-day (Group1) and 40-day periods (Group2)) and healthy group (Group3). At the end of experimental period, rats were sacrificed, and radiological and histomorphometric analyses were performed on the mandibles. PTX3 levels were measured in gingival tissue and serum samples using ELISA. Plasma fibrinogen levels were measured according to the nephelometric method.Results.Significant alveolar bone resorption and periodontal inflammation were evident in periodontitis groups. Levels of PTX3 in gingival tissue were statistically higher in Group 1 than those in groups 2 and 3 (P<0.01). No significant difference was found in serum PTX3 levels between experimental periodontitis and control groups (P>0.05). Plasma fibrinogen levels were significantly increased in the experimental periodontitis groups (P<0.001).Conclusion.PTX3 seems to be associated with tissue destruction in earlier periods of inflammatory periodontal disease, contrary to the fibrinogen findings.

scholarly journals Antiperiodontitis Effects of Magnolia biondii Extract on Ligature-Induced Periodontitis in Rats

Nutrients ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 934 ◽  
Author(s):  
Hae Jin Lee ◽  
Dong-Ryung Lee ◽  
Bong-Keun Choi ◽  
Seung Hwan Yang
Keyword(s):  
Bone Loss ◽  
Tissue Damage ◽  
Alveolar Bone ◽  
Histopathological Examination ◽  
Nuclear Factor Κb ◽  
Alveolar Bone Loss ◽  
Gingival Tissue ◽  
Protective Effects ◽  
Tissue Destruction ◽  
Commercial Kits

Over the past decades, periodontitis has become a rising health problem and caused various diseases. In the many studies shows that some extracts and compound to the prevention and treatment of periodontitis. This study focuses on the effects of inhibition of gingival damage and alveolar bone loss. The aim of this study was to evaluate the protective effects of Magnolia biondii extract (MBE) against ligature-induced periodontitis in rats. A ligature was placed around the molar teeth for 8 weeks, and MBE was administered for 8 weeks. Gingival tissue damage and alveolar bone loss were measured by microcomputed tomography (CT) analysis and histopathological examination. Serum Interluekin-1 β (IL-1β), tumor necrosis factor-α (TNF-α), cyclooxygenases-2 (COX-2), and receptor activator of nuclear factor–κB ligand (RANKL) levels were investigated using commercial kits to confirm the antiperiodontitis effects of MBE. We confirmed that ligature-induced periodontitis resulted in gingival tissue damage and alveolar bone loss. However, treatment for 8 weeks with MBE protected from periodontal tissue damage and downregulated serum inflammatory cytokine factors and RANKL levels. These results suggest that MBE exerts antiperiodontitis effects by inhibiting gingival tissue destruction and alveolar bone loss through regulation of anti-inflammatory cytokines in periodontitis-induced rats.

scholarly journals Blood Oxidative Stress Modulates Alveolar Bone Loss in Chronically Stressed Rats

2020 ◽  
Vol 21 (10) ◽  
pp. 3728 ◽  
Author(s):  
Micaele Maria Lopes Castro ◽  
Priscila Cunha Nascimento ◽  
Deiweson Souza-Monteiro ◽  
Sávio Monteiro Santos ◽  
Mayra Barros Arouck ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Alveolar Bone ◽  
Physical Restraint ◽  
Thiobarbituric Acid ◽  
Experimental Period ◽  
Thiobarbituric Acid Reactive Substance ◽  
Micro Ct ◽  
Plus Maze ◽  
Behavioral Parameters ◽  
Experimental Periodontitis

We aimed to investigate the effects of chronic stress (CS) on experimental periodontitis (EP) in rats. For this, 28 Wistar rats were divided into four groups: control, ligature-induced experimental periodontitis (EP), chronic stress (CS; by physical restraint model) and CS+EP (association of chronic stress and ligature-induced periodontitis). The experimental period lasted 30 days, including exposure to CS every day and ligature was performed on the 15th experimental day. After 30 days, the animals were submitted to the behavioral test of the elevated plus maze (EPM). Next, rats were euthanized for blood and mandible collection in order to evaluate the oxidative biochemistry (by nitric oxide (NO), reduced-glutathione activity (GSH), and thiobarbituric acid reactive substance levels (TBARS)) and alveolar bone characterization (by morphometric, micro-CT, and immunohistochemistry), respectively. The behavioral parameters evaluated in EPM indicated higher anxiogenic activity in the CS and CS+EP, groups, which is a behavioral reflex of CS. The results showed that CS was able to change the blood oxidative biochemistry in CS and CS+EP groups, decrease GSH activity in the blood, and increase the NO and TBARS concentrations. Thus, CS induces oxidative blood imbalance, which can potentialize or generate morphological, structural, and metabolic damages to the alveolar bone.

Cannabidiol decreases bone resorption by inhibiting RANK/RANKL expression and pro-inflammatory cytokines during experimental periodontitis in rats

2009 ◽  
Vol 9 (2) ◽  
pp. 216-222 ◽  
Author(s):  
Marcelo H. Napimoga ◽  
Bruno B. Benatti ◽  
Flavia O. Lima ◽  
Polyanna M. Alves ◽  
Alline C. Campos ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Inflammatory Cytokines ◽  
Rankl Expression ◽  
Experimental Periodontitis ◽  
Pro Inflammatory Cytokines

scholarly journals The effect of sword bean extract on the relationship between rheumatoid arthritis and periodontitis in mice

2019 ◽  
Author(s):  
Kosei Matsumoto ◽  
Yuko Nakatsuka ◽  
Kaname Shirai ◽  
Shintaro Shimizu ◽  
Shunshuke Yanase ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Bone Resorption ◽  
Alveolar Bone ◽  
Cathepsin K ◽  
Joint Inflammation ◽  
Tissue Destruction ◽  
Tissue Inflammation ◽  
Canavalia Gladiata ◽  
Experimental Mouse ◽  
The Relationship

AbstractObjectivesSeveral studies in humans and experimental animals have reported an interaction between rheumatoid arthritis (RA) and periodontitis (PD). We previously showed that extracts of Canavalia gladiata (sword bean extract, SBE) can treat PD in rats. Here, we investigated the relationship between RA and PD and the effects of SBE in an experimental mouse model.MethodsFemale SKG mice were assigned to eight groups (n=6/group): (1) Untreated controls, (2) RA (induced at 6 weeks of age), (3) PD (induced at 10 weeks of age), (4) RA + PD, (5) SBE (2 mg/ml in drinking water starting at 5 weeks of age), (6) PD + SBE, (7) RA + SBE, and (8) RA + PD + SBE. Mice were sacrificed at 13 weeks of age, and alveolar bone resorption, periodontal tissue inflammation, and paw joint inflammation were assessed by histology and immunohistochemistry.ResultsMice in the RA + PD group exhibited significantly higher inflammation scores in the joint tissues as well as more abundant IL-17-positive cells and cathepsin K-positive osteoclasts in the radial bone compared with the RA mice. Alveolar bone resorption was also significantly more severe in the RA + PD mice than in the PD mice. SBE treatment significantly improved all bone resorption and tissue inflammation scores in mice with RA + PD.ConclusionConcomitant RA and PD exacerbates the tissue destruction symptomatic of each condition. SBE suppresses all parameters evaluated, suggesting that it is has anti-inflammatory activities in both RA and PD.

A probiotic combination attenuates experimental colitis through inhibition of innate cytokine production

2017 ◽  
Vol 8 (2) ◽  
pp. 231-241 ◽  
Author(s):  
M.S. Kim ◽  
J.S. Byun ◽  
Y.S. Yoon ◽  
D.Y. Yum ◽  
M.J. Chung ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Immune Cell ◽  
Therapeutic Potential ◽  
Lactobacillus Rhamnosus ◽  
Experimental Colitis ◽  
Intestinal Bleeding ◽  
Myeloperoxidase Activity ◽  
Tissue Destruction ◽  
Raw264.7 Macrophages ◽  
Pro Inflammatory Cytokines

Inflammatory bowel disease (IBD) is a severe immune cell-mediated syndrome characterised by extensive inflammatory and effector mucosal responses leading to tissue destruction in the colon and small intestine. The leading hypothesis is that dysbiosis of the gut flora causes an excessive immune response and inflammation in the gastrointestinal track. Lactic acid bacteria (LAB) can correct dysbiosis of the normal microbiota. In the current study, the therapeutic potential of seven LAB strains in combination to treat IBD was evaluated using experimental colitis model. This LAB cocktail, designated GI7, includes four strains of Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactococcus lactis, two strains of Bifidobacterium bifidum, Bifidobacterium breve, and one strain of Streptococcus thermophilus. We confirmed that GI7 suppressed pro-inflammatory cytokines in Raw264.7 macrophages. When dextran sulphate sodium-induced colitic mice were treated with GI7, their symptoms of colitis, as assessed by body weight, colon length, myeloperoxidase activity, intestinal bleeding, and histological damage, were reduced compared to untreated mice. In addition, GI7 treatment significantly inhibited the production of innate pro-inflammatory cytokines during colitic progression. Therefore, we suggest that GI7, a combination of seven LAB, has a potential role in the treatment of IBD.

scholarly journals Deferoxamine Reduces Inflammation and Osteoclastogenesis in Avulsed Teeth

2021 ◽  
Vol 22 (15) ◽  
pp. 8225
Author(s):  
Ko Eun Lee ◽  
Mijeong Jeon ◽  
Seunghan Mo ◽  
Hyo-Seol Lee ◽  
Je Seon Song ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Root Surface ◽  
Tartrate Resistant Acid Phosphatase ◽  
Micro Ct ◽  
Sprague Dawley ◽  
Micro Computed Tomography ◽  
Model Cell

Replacement and inflammatory resorption are serious complications associated with the delayed replantation of avulsed teeth. In this study, we aimed to assess whether deferoxamine (DFO) can suppress inflammation and osteoclastogenesis in vitro and attenuate inflammation and bone resorption in a replanted rat tooth model. Cell viability and inflammation were evaluated in RAW264.7 cells. Osteoclastogenesis was confirmed by tartrate-resistant acid phosphatase staining, reactive oxygen species (ROS) measurement, and quantitative reverse transcriptase–polymerase chain reaction in teeth exposed to different concentrations of DFO. In vivo, molars of 31 six-week-old male Sprague–Dawley rats were extracted and stored in saline (n = 10) or DFO solution (n = 21) before replantation. Micro-computed tomography (micro-CT) imaging and histological analysis were performed to evaluate inflammation and root and alveolar bone resorption. DFO downregulated the genes related to inflammation and osteoclastogenesis. DFO also reduced ROS production and regulated specific pathways. Furthermore, the results of the micro-CT and histological analyses provided evidence of the decrease in inflammation and hard tissue resorption in the DFO group. Overall, these results suggest that DFO reduces inflammation and osteoclastogenesis in a tooth replantation model, and thus, it has to be further investigated as a root surface treatment option for an avulsed tooth.

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