scholarly journals Effects of Gelam Honey (Melaleuca cajuputi) on Alveolar Bone Loss in Experimental Periodontitis

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
N. Hamzah ◽  
S. A. Aziz ◽  
A. R. Fauzi ◽  
Y. A. Mohd Yusof ◽  
M. Razali ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Melaleuca Cajuputi ◽  
Significant Difference ◽  
Gelam Honey ◽  
Bone Level ◽  
Experimental Periodontitis

Gelam honey has been shown to exhibit antioxidant and anti-inflammatory activities in animal model. The aim of this study was to determine the effects of Gelam honey (Melaleuca cajuputi) on alveolar bone level in experimental periodontitis. Thirty male Sprague-Dawley rats were used in this study and randomly divided into four groups: ligated saline (LS), ligated honey (LH), nonligated saline (NLS), and nonligated honey (NLH). Fifteen days after supplementation with Gelam honey (3 g/kg), the rats were sacrificed and alveolar bone level was determined by radiography and histomorphometry. The number of osteoclasts was also calculated for all groups. Both radiographic and histomorphometric analyses showed that alveolar bone resorption was severely induced around the ligated molar in the LS and LH groups. There was no significant difference in alveolar bone level between the LS and LH groups. However, there was a nonsignificant reduction of osteoclast number by 15.2% in LH group compared to LS group. In the NLH group, there was less alveolar bone resorption and the number of osteoclasts was reduced by 13.2% compared to NLS group. In conclusion, systemically supplemented Gelam honey was shown to have the potential of reducing osteoclast activity in the experimental periodontitis rats, even though the effect on alveolar bone level was not well demonstrated and it warrants further research.

Effects of Polycan, a β-glucan, on experimental periodontitis and alveolar bone loss in Sprague-Dawley rats

2012 ◽  
Vol 47 (6) ◽  
pp. 800-810 ◽  
Author(s):  
Y. S. Kim ◽  
S. J. Kang ◽  
J. W. Kim ◽  
H. R. Cho ◽  
S. B. Moon ◽  
...  
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Experimental Periodontitis

scholarly journals The combination of carbonate hydroxyapatite and human β-defensin 3 to enhance collagen fibre density in periodontitis Sprague Dawley rats

2020 ◽  
Vol 53 (2) ◽  
pp. 76
Author(s):  
Ika Andriani ◽  
Edy Meiyanto ◽  
S. Suryono ◽  
Ika Dewi Ana
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Healing Process ◽  
Collagen Fibre ◽  
Control Group ◽  
Sprague Dawley ◽  
Carbonate Hydroxyapatite ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Collagen Density

Background: Carbonate hydroxyapatite (CHA) is used as a scaffold to repair bone resorption. Alveolar bone resorption in periodontitis caused by an infection requires the presence of an antibacterial to support bone regeneration. Human β-defensin 3 (HBD3) is an antimicrobial peptide. The local application of the HBD3 antimicrobial is beneficial to inhibiting drug resistance and protecting tissue regeneration against invasive bacteria. Purpose: This study aims to investigate the effect of the administration of a combination of   CHA with HBD3 on the collagen density of periodontitis rats (Sprague Dawley). Methods: This study was a true experimental study with a post-test control group design. Thirty-two Sprague Dawley animal models were randomly blind selected placed under anaesthetic, then a 2-mm silk ligature was attached as a ligation to the mandibular incisors for 14 days in order to generate periodontitis. The study subjects were divided into two groups, the group with CHA and CHA loaded HBD3 (CHA + HBD3) implantation. On days 7, 14, 21 and 28, four rats were taken randomly from each group for decapitation, followed by histological processing and examination with trichome Mallory staining. The data was analysed using the Kruskal Wallis test (p<0.05). Results: An increase in collagen density during the healing process was found. There was a significant difference between CHA and CHA+HBD (p=0.004 and p=0.008; p<0.05) in collagen density between the groups. Conclusion: The combination of CHA and HBD3 can enhance the collagen density in periodontitis Sprague Dawley rats, compared to CHA only groups.

FSH Aggravates Periodontitis-related Bone Loss in Ovariectomized Rats

2010 ◽  
Vol 89 (4) ◽  
pp. 366-371 ◽  
Author(s):  
S. Liu ◽  
Y. Cheng ◽  
M. Fan ◽  
D. Chen ◽  
Z. Bian
Keyword(s):  
Bone Resorption ◽  
Bone Loss ◽  
Clinical Features ◽  
Alveolar Bone ◽  
Follicle Stimulating Hormone ◽  
Alveolar Bone Loss ◽  
Ovariectomized Rats ◽  
Fsh Receptor ◽  
Histochemical Tests ◽  
Experimental Periodontitis

Alveolar bone loss is one of the prominent pathologic and clinical features of periodontitis. Recently, the direct effect of follicle-stimulating hormone (FSH) on bone resorption has been demonstrated. However, the effect of FSH on alveolar bone loss remains unknown. This study tested the hypothesis that FSH would exacerbate periodontitis-related alveolar bone loss. Experimental periodontitis was induced in ovariectomized rats, and the rats were treated with extrinsic FSH or its inhibitor, leuprorelin. After mandibles were collected, we performed morphological examinations to evaluate bone loss, enzyme histochemical tests for osteoclasts, and immunohistochemical examinations for FSH receptor (FSHR). The results showed that FSH significantly increased alveolar bone resorption compared with non-FSH-treated ovariectomized rats (P < 0.05), and the number of FSHR-positive cells was positively correlated with alveolar bone loss area (r = 0.682, P < 0.01). Our results suggested that FSH can aggravate alveolar bone loss by FSHR, independent of estrogen.

scholarly journals Boric acid inhibits alveolar bone loss in rat experimental periodontitis through diminished bone resorption and enhanced osteoblast formation

2020 ◽  
Vol 15 (4) ◽  
pp. 437-444
Author(s):  
Nazmus Shalehin ◽  
Akihiro Hosoya ◽  
Hiroaki Takebe ◽  
Md Riasat Hasan ◽  
Kazuharu Irie
Keyword(s):  
Bone Resorption ◽  
Bone Loss ◽  
Boric Acid ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Experimental Periodontitis

scholarly journals In-VivoEffect of Andrographolide on Alveolar Bone Resorption Induced byPorphyromonas gingivalisand Its Relation with Antioxidant Enzymes

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Rami Al Batran ◽  
Fouad H. Al-Bayaty ◽  
Mazen M. Jamil Al-Obaidi
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Reduced Glutathione ◽  
Serum Levels ◽  
Normal Control Group ◽  
Control Group ◽  
Healthy Male ◽  
Sprague Dawley ◽  
Bone Level ◽  
Left And Right

Alveolar bone resorption is one of the most important facts in denture construction.Porphyromonas gingivalis(Pg) causes alveolar bone resorption, and morphologic measurements are the most frequent methods to identify bone resorption in periodontal studies. This study has aimed at evaluating the effect of Andrographolide (AND) on alveolar bone resorption in rats induced byPg. 24 healthy maleSprague Dawleyrats were divided into four groups as follows: normal control group and three experimental groups challenged orally withPgATCC 33277 five times a week supplemented with 20 mg/kg and 10 mg/kg of AND for twelve weeks. Alveolar bones of the left and right sides of the mandible were assessed by a morphometric method. The bone level, that is, the distance from the alveolar bone crest to cementumenamel junction (CEJ), was measured using 6.1 : 1 zoom stereomicroscope and software. AND reduced the effect ofPgon alveolar bone resorption and decreased the serum levels of Hexanoyl-Lysine (HEL); furthermore the reduced glutathione/oxidised glutathione (GSH/GSSG) ratio in AND treated groups (10 and 20 mg/kg) significantly increased when compared with thePggroup(P<0.05). We can conclude that AND suppresses alveolar bone resorption caused byPgin rats.

Resorption of Residual Ridges (RRR) in Rats

1987 ◽  
Vol 66 (12) ◽  
pp. 1753-1757 ◽  
Author(s):  
I. Nishimura ◽  
P.J. Damiani ◽  
D.A. Atwood
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Minimal Trauma ◽  
Specific Factors ◽  
Maxilla And Mandible ◽  
The Right ◽  
Mandibular First Molar

The post-extraction resorption of residual ridges (RRR) is a major and largely unsolved health problem, probably of multifactorial etiology. In order for one to study the role of specific factors in the pathophysiology of RRR, the use of animal models is desirable. The purpose of this study was to establish a reliable animal model and a standardized assay system to measure RRR for future experiments. A new oblique cephalometric device was designed to take pairs of xeroradiographs on the right and left sides of the rat skull and mandible at a 45-degree angle to the horizontal plane. Preliminary studies confirmed the reproducibility of the technique. All molars were extracted with minimal trauma from the right maxilla and mandible in five male Sprague-Dawley rats (40 days old). Longitudinal cephalographic examinations were performed before and immediately after extraction and at two, four, eight, and 12 weeks after extraction. Alveolar bone resorption was measured on enlarged cephalographs (5. 7X) at a point mesial to the mandibular first molar. Sequential mean bone resorption was 0.8 ± 0.2 mm (S.D.), 1.0 ± 0.3, 1.3 ± 0.3, and 1.5 ± 0.4, respectively. Graphically, these findings produced bone loss curves similar to those observed in man. These results indicate that the rat model may be utilized in longitudinal studies of the resorption of the residual ridge.

scholarly journals Effect of jackfruit seed fortification on high calcium milk on bone collagen density

2019 ◽  
Vol 4 (2) ◽  
pp. 82
Author(s):  
Aulia Ayub ◽  
Fun Nagede Adinsyah ◽  
Arum Trisnaningtyas Sugiyanto Putri ◽  
Presty Dwi Fitriani ◽  
Risma Martasuri ◽  
...  
Keyword(s):  
Collagen Fiber ◽  
Alveolar Bone ◽  
Seed Extract ◽  
Bone Collagen ◽  
Control Group ◽  
Sprague Dawley ◽  
Fiber Density ◽  
Sprague Dawley Rats ◽  
High Calcium ◽  
Significant Difference

Osteoporosis is a bone disease characterized by reduced bone mass and damage to the microarchitecture of bone tissue. One of osteoporosis types is primary type-1 osteoporosis or postmenopausal osteoporosis due to estrogen deficiency. Calcium is an important mineral that plays a central role in the formation of bone strength structure. Jackfruit seeds (Artocarpus heterophyllus) contain isoflavones that can replace the role of estrogen in the body. Fortification of jackfruit seeds and high calcium milk can be a potential alternative for the treatment of postmenopausal osteoporosis. The purpose of this study was to determine the effect of drinking high calcium milk fortified with jackfruit seed extract (Artocarpus heterophyllus) on alveolar bone collagen fiber density of menopause induced rats. Twenty-five Sprague dawley rats were included in this study. Twenty Sprague dawley rats were ovariectomized, while 5 rats were left untreated (normal control group). Seven days after ovariectomy was performed, 5 rats were treated with fortification of jackfruit seed extract and high calcium milk, 5 rats were treated with jackfruit seed extract, 5 rats were treated with high calcium milk, and 5 rats were left untreated (ovariectomized control group). The treatments were done for 28 days. The rats were euthanized and the alveolar bone was taken and processed for a histological specimen. Alveolar bone collagen fibers were observed under a binocular microscope with 100 magnification. Kruskal-Wallis result showed there was a significant difference in the alveolar bone collagen fiber density among these groups (p<0.05). Mann-Whitney U Test showed a significant difference (p<0.05) in the alveolar bone collagen fiber density of the rats treated with fortification of jackfruit seed extract and high calcium milk compared to the group treated with jackfruit seed extract, that with high calcium milk, or ovariectomized control group. No significant difference in alveolar bone collagen fiber density was found between the group treated with fortification of jackfruit seed extract and high calcium milk and the normal control group (p>0.05). In conclusion, consuming high calcium milk fortified with jackfruit seed extract increases the density of alveolar bone collagen fibers.

3,4,5-Trihydroxybenzoic acid attenuates ligature-induced periodontal disease in Wistar rats.

2020 ◽  
Vol 19 ◽  
Author(s):  
Ozkan Karatas ◽  
Fikret Gevrek
Keyword(s):  
Bone Loss ◽  
Gallic Acid ◽  
Wistar Rats ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Collagenase Activity ◽  
Osteoblastic Activity ◽  
Cell Counts ◽  
Experimental Periodontitis ◽  
Anti Inflammatory

Background: 3,4,5-Trihydroxybenzoic acid, which is also known as gallic acid, is an anti-inflammatory agent who could provide beneficial effects in preventing periodontal inflammation. The present study aimed to evaluate the anti-inflammatory effects of gallic acid on experimental periodontitis in Wistar rats. Alveolar bone loss, osteoclastic activity, osteoblastic activity, and collagenase activity were also determined. Methods: 32 Wistar rats were used in the present study. Study groups were created as following: Healthy control (C,n=8) group; periodontitis (P,n=8) group; periodontitis and 30 mg/kg gallic acid administered group (G30,n=8); periodontitis and 60 mg/kg gallic acid administered group (G60,n=8). Experimental periodontitis was created by placing 4-0 silk sutures around the mandibular right first molar tooth. Morphological changes in alveolar bone were determined by stereomicroscopic evaluation. Mandibles were undergone histological evaluation. Matrix metalloproteinase (MMP)-8, tissue inhibitor of MMPs (TIMP)-1, bone morphogenetic protein (BMP)-2 expressions, tartrate-resistant acid phosphatase (TRAP) positive osteoclast cells, osteoblast, and inflammatory cell counts were determined. Results: Highest alveolar bone loss was observed in the periodontitis group. Both doses of gallic acid decreased alveolar bone loss compared to the P group. TRAP-positive osteoclast cell counts were higher in the P group, and gallic acid successfully lowered these counts. Osteoblast cells also increased in gallic acid administered groups. Inflammation in the P group was also higher than those of C, G30, and G60 groups supporting the role of gallic acid in preventing inflammation. 30 and 60 mg/kg doses of gallic acid decreased MMP-8 levels and increased TIMP-1 levels. BMP levels increased in gallic acid administered groups, similar to several osteoblasts. Conclusion: Present results revealed an anti-inflammatory effect of gallic acid, which was indicated by decreased alveolar bone loss and collagenase activity and increased osteoblastic activity.

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