scholarly journals Phytochemicals Content, Antioxidant Activity and Acetylcholinesterase Inhibition Properties of IndigenousGarcinia parvifoliaFruit

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Siti Hawa Ali Hassan ◽  
Jeffrey R. Fry ◽  
Mohd Fadzelly Abu Bakar
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging ◽  
Acetylcholinesterase Inhibition ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Garcinia Mangostana ◽  
Total Carotenoid Content ◽  
Freeze Dried ◽  
Gallic Acid Equivalent ◽  
Phenolic And Flavonoid

Garcinia parvifoliabelongs to the same family as mangosteen (Garcinia mangostana), which is known locally in Sabah as “asam kandis” or cherry mangosteen. The present study was conducted to determine the phytochemicals content (total phenolic, flavonoid, anthocyanin, and carotenoid content) and antioxidant and acetylcholinesterase inhibition activity of the flesh and peel ofG. parvifolia. All samples were freeze-dried and extracted using 80% methanol and distilled water. For the 80% methanol extract, the flesh ofG. parvifoliadisplayed higher phenolic and flavonoid contents than the peel, with values of7.2±0.3 mg gallic acid equivalent (GAE)/g and5.9±0.1 mg rutin equivalent (RU)/g, respectively. Anthocyanins were detected in the peel part ofG. parvifoliabut absent in the flesh. The peel ofG. parvifoliadisplayed higher total carotenoid content as compared to the flesh part with the values of17.0±0.3and3.0±0.0 mgβ-carotene equivalents (BC)/100 g, respectively. The free-radical scavenging, ferric reducing, and acetylcholinesterase inhibition effect of the flesh were higher as compared to the peel in both extracts. These findings suggested that the edible part ofG. parvifoliafruit has a potential as a natural source of antioxidant and anti-Alzheimer’s agents.

scholarly journals Assessing the Potential of Algae Extracts for Extending the Shelf Life of Rainbow Trout (Oncorhynchus mykiss) Fillets

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 910
Author(s):  
María I. Sáez ◽  
María D. Suárez ◽  
Francisco J. Alarcón ◽  
Tomás F. Martínez
Keyword(s):  
Rainbow Trout ◽  
Cold Storage ◽  
Haematococcus Pluvialis ◽  
Radical Scavenging ◽  
Storage Period ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Color Parameters ◽  
Total Carotenoid Content

This study evaluates the potential of different algae extracts (Crassiphycus corneus, Cc; Ulva ohnoi, Uo; Arthrospira platensis, Ap; Haematococcus pluvialis, Hp) as additives for the preservation of rainbow trout fillets. The extracts were prepared with different water to ethanol ratios from the four algae species. The highest ferric reducing antioxidant power (FRAP) was observed in Uo extracted in 80% ethanol. Ap aqueous extract also had considerable FRAP activity, in agreement with a high total phenolic content. Radical scavenging activity (DPPH) was higher in Cc 80% ethanol extract, in agreement with a high total carotenoid content. In fact, when the algae aqueous extracts were assayed on the fish fillets, their antioxidant activity exceeded that of ascorbic acid (ASC). All algae extracts delayed microbial growth and lipid oxidation processes in trout fillets throughout the cold storage period compared to controls, and also improved textural parameters, these effects being more evident for Ap and Hp. With respect to the color parameters, the Hp extract prevented the a* values (redness) from decreasing throughout cold storage, a key point when it comes to colored species, not least salmonids. On the other hand, the Ap extract was not as effective as the rest of treatments in avoiding a* and b* decrease throughout the storage period, and thereby the color parameters were impaired. The results obtained, together with the natural origin and the viability for large-scale cultivation, make algae extracts interesting fish preservative agents for the food industry.

scholarly journals Assessment of Total Phenolic, Flavonoid Content and Antioxidant Activity of Ocimum sanctum Linn

2019 ◽  
Vol 40 ◽  
pp. 30-35
Author(s):  
Ishwor Pathak ◽  
Muna Niraula
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Ocimum Sanctum ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Phenolic And Flavonoid

Ocimum sanctum, commonly known as Tulasi in Nepal, is a pharmacologically important plant due to its active constituents. In this work, extraction was carried out in hexane, chloroform and methanol solvents and their phytochemical screening was performed. Total phenolic and flavonoid contents in the plant were measured by Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method respectively. Antioxidant activity of the extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Alkaloids, flavonoids, tannins, glycosides, polyphenols, terpenoids, tannins and steroids are mainly found in the extracts. Based on the result obtained, the plant posses a significant amount of total phenolic and total flavonoid content. Both phenolic and flavonoid contents were highest in methanol extract, followed by chloroform and hexane extract. Antioxidant activity of the extracts as ascorbic acid standard (IC50 value = 41.34 µg/mL) was in the order of methanol extract (IC50 value = 47.73 µg/mL) > chloroform extract (IC50 value = 79.46 µg/mL) > hexane extract (IC50 value = 94.68 µg/mL). The extent of the antioxidant activity of the plant is following the number of total phenolics and flavonoids present in it.

scholarly journals Estimation of Total Phenolic and Flavonoid Contents in Some Medicinal Plants and Their Antioxidant Activities

2015 ◽  
Vol 15 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Bedraj Pandey ◽  
Meena Rajbhandari
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Aqueous Acetone ◽  
Total Phenolic ◽  
Aqueous Methanol ◽  
Acetone Extracts ◽  
Phenolic And Flavonoid

Quercetin was isolated from ethyl acetate fraction of a methanol extract of the outer scale of onion by repeated sephadex LH-20 column chromatography. Methanol, 50% aqueous methanol and 70% aqueous acetone extracts of different parts of four medicinal plants, Ficus benghalensis, Elaeocarpus sphaericus, Ipomea carnea and Azeratum conyzoides were prepared and screened for total phenolic and flavonoid contents and free radical scavenging activity. The total phenolics contents were measured spectrophotometrically by using Folin-Ciocalteu reagent and total flavonoids content by using aluminum chloride colorimetric method. Free radical scavenging activity was evaluated using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. All the investigated medicinal plant extracts contained high amount of phenolics. The highest amount was detected in 70% aqueous acetone extract of E. sphaericus (298.8±9.03 mg GAE/g extract) and lowest amount in 50% aqueous methanol extract of F. benghalensis (6.7±0.73 mg GAE/g extract). The highest amount of flavonoid was found in methanol extract (78.2±2.72 mg quercetin/g extract) and the lowest amount was detected in 50% aqueous methanol extract (2.1±0.25 mg quercetin/g extract) of F. benghalensis. DPPH free radical scavenging assay was carried out only in 70% acetone extracts. The highest IC50 value was observed for E. sphaericus (34.0 μg/ml). A strong linear correlation between total phenolic content and antioxidant activity was found indicating that the major antioxidants are phenolics.DOI: http://dx.doi.org/10.3126/njst.v15i1.12010  Nepal Journal of Science and Technology Vol. 15, No.1 (2014) 53-60

scholarly journals Antioxidant activity and total phenolic and flavonoid contents of Salvia amplexicaulis Lam. extracts

2014 ◽  
Vol 66 (1) ◽  
pp. 307-316 ◽  
Author(s):  
Ana Alimpic ◽  
Mariana Oaldje ◽  
V. Matevski ◽  
P.D. Marin ◽  
Sonja Duletic-Lausevic
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Methanol Extracts ◽  
Whole Plant ◽  
Phenolic And Flavonoid

This study was designed to examine the in vitro antioxidant DPPH free radical-scavenging activity, and the total phenolic and flavonoid content of ethanol and methanol extracts of Salvia amplexicaulis Lam. in the whole plant and different parts, leaves, stems and flowers. The largest amounts of extract yield were obtained from the flowers, 14.14% and 12.00 % (w/w) in the ethanol of methanol extracts, respectively. The ethanol extract of leaves (16.07 ?g/ml) and methanol extract of the whole plant (21.28 ?g/ml) showed the highest activity against the DPPH radical. The ethanol extract of the leaves was the richest in phenols (222.40 mg GAE/g) and flavonoids (49.81 mg QE/g), whereas the methanol extract of the whole plant contained the highest amount of phenolics (180.89 mg GAE/g) and flavonoids (38.15 mg QE/g). A very strong linear correlation between antioxidant activity and the phenolic content of the extracts was established. The obtained results suggest that S. amplexicaulis could be regarded as an important source of natural antioxidants.

scholarly journals Effects of drying methods on the physicochemical properties and antioxidant capacity of Kuini powder

2021 ◽  
Vol 24 ◽  
Author(s):  
Gan Wei Shuen ◽  
Lew Yan Yi ◽  
Thor Sing Ying ◽  
Germaine Chng Yu Von ◽  
Yus Aniza Binti Yusof ◽  
...  
Keyword(s):  
Freeze Drying ◽  
Colour Change ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Drying Methods ◽  
Vacuum Oven ◽  
Oven Drying ◽  
Convection Oven ◽  
Total Carotenoid Content ◽  
Freeze Dried

Abstract Kuini powders were obtained through spray-drying, freeze-drying, vacuum oven drying and convection oven drying. Colour, water activity (Aw), moisture content, hygroscopicity, wettability, flowability, degree of caking, water solubility index (WSI), total colour change, total phenolic content and total carotenoid content of Kuini powders were determined and compared. Convection oven drying resulted in the highest yield (46.97%), moisture content (4.91%), Aw (0.55) and WSI (74.33%) among all the drying methods. However, convection oven-dried Kuini powder had the lowest hygroscopicity and wettability, which were 18.66% and 12.04 s, respectively. Spray drying resulted in poor hygroscopicity (22.41%), degree of caking (22.16%), wettability (275s), WSI (45.67%) and higher colour change (59.81). Least total colour change (19.05) and higher yellowness (57.31) were observed in freeze-drying. In addition, freeze-dried Kuini powder had the highest total phenolic content (24.76 mg/100 g) and total carotenoid content (1.61 mg/100 g). Drying temperature had a negative and significant correlation with the retention of color pigments and antioxidant content. The physicochemical properties and antioxidant content of freeze-dried Kuini powder were preferred over spray-dried, vacuum oven-dried and convection oven-dried powders. Hence, freeze-drying offers potential application in the food products.

scholarly journals Phytochemical Evaluation of Some Medicinal Plants of Puthan District of Nepal

2020 ◽  
Vol 19 (1) ◽  
pp. 97-106
Author(s):  
Hari Giri ◽  
Meena Rajbhandari
Keyword(s):  
Medicinal Plants ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Aqueous Acetone ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid ◽  
Different Parts

Different parts of eleven medicinal plants used in the traditional medicine in Puthan district were collected. Three different extracts, methanol, 50% aqueous methanoland 70% aqueous acetone extractswere prepared for each plant material. The methanol extractswere screened for the presence of different classes of phytochemicals. Total phenolic andflavonoid content, as well as DPPH freeradical scavenging activity, were evaluated. All the investigated extracts contain a high amount of phenolics and flavonoids.The highest amount of phenolicsand flavonoids were detected in the methanolextract of bark of Bauhinia variegate(355.35+3.69 mg GAE/g and 209.23 ±1.25 mg QE/g extract). Among the eight tested extracts, the highest radical scavenging activity was shown by methanol extract of bark of B. variegate (IC50 3.68 μg/ml). The extract having the highest phenolic and flavonoid content showed the lowest IC50 demonstrating the positive correlation between radical scavenging activity and total phenolic and flavonoid content.

scholarly journals ANTIOXIDATIVE AND FREE RADICAL SCAVENGING POTENTIALS OF CYCLOSORUS INTERRUPTUS (WILLD.) H. ITÔ AND PRONEPHRIUM NUDATUM (ROXB. EX GRIFF.) HOLTTUM

2019 ◽  
pp. 87-94
Author(s):  
ABHIJIT MITRA ◽  
MANABENDRA DUTTA CHOUDHURY ◽  
PRAKASH ROY CHOUDHURY ◽  
DEEPA NATH ◽  
SUBRATA DAS ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
In Vitro Systems ◽  
Reducing Power Assay ◽  
Phenolic And Flavonoid

Objectives: The work aims to screen the antioxidative potentials of different crude extracts of the fronds of two medicinally important pteridophytes of Southern Assam, India, namely, Cyclosorus interruptus (Willd.) H. Itô and Pronephrium nudatum (Roxb. ex Griff.) Holttum. Methods: Frond extracts of the pteridophytes were prepared by Soxhlet hot extraction method. Total phenolic content (TPC) and total flavonoid content (TFC) of the hexane, ethyl acetate, acetone, and methanol extracts of the fronds of the plants were done by following standard protocol. In vitro assessment of the antioxidative behavior of the extracts was performed using standard 2,2-diphenyl-1-picrylhydrazyl scavenging assay, reducing power assay, metal chelating assay, hydroxyl, superoxide, and 2,2’- azino-bis (3-ethylbenzothiazoline - 6 – sulfonic acid) radical scavenging methods in different in vitro systems. Results: Preliminary phytochemical analysis implicated the presence of phenolic and flavonoid compounds in all the frond extracts. The methanol extract of the fronds of both the plants showed maximum phenolic and flavonoid contents in comparison to the other extracts, however, that of C. interruptus was found to be higher than P. nudatum. Antioxidative potentials of the said extracts were also found to be impressive and noteworthy. The decreasing order of the antioxidative efficacies of the extracts was found to be same as that of TPC and TFC of the extracts. Conclusion: It is pertinent to comment that the methanol extract of the fronds of both the plants may be treated as a potential source of natural antioxidants.

scholarly journals Evaluation of Phenolic and Flavonoid Content, Antioxidant and Radical Scavenging Capacity of Baru (Dipteryx alata Vog.) Almond

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 326-326
Author(s):  
Deja Ivy ◽  
Rami Najjar ◽  
Maureen Meister ◽  
Maria Margareth Naves ◽  
Rafaela Feresin
Keyword(s):  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Freeze Dried ◽  
Trolox Equivalent ◽  
Phenolic And Flavonoid ◽  
Dipteryx Alata

Abstract Objectives The legume plant species Dipteryx alata Vog., commonly known as baru, is a tree that grows in the Brazilian savanna. It produces drupe type of fruit and has an edible seed referred to as the baru nut. The baru nut is of interest as it possesses bioactive compounds useful in managing chronic diseases, yet little is known about its composition. Thus, the aim of this study was to determine the phenolic and flavonoid content, as well as antioxidant and radical scavenging capacity of the baru nut. Methods Baru nuts were roasted, pulverized, and freeze-dried prior to polyphenolic extractions, which were performed using 80% ethanol and ultrasonic bath. Baru crude extracts were then purified with chloroform, evaporated, and freeze-dried prior to being assayed. Total phenolic content and total flavonoid content of purified baru polyphenolic extracts were determined using Folin-Ciocalteu reagent and aluminum chloride colorimetric assay, respectively. Antioxidant capacity was measured using Trolox equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant power (FRAP) while 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) assays were used to determine radical scavenging capacity. Results Total phenolic content of purified baru nut extract was 58 ± 10 μmol gallic acid equivalent/L while total flavonoid content was 7 ± 0 μg quercetin equivalent/mL. The TEAC of purified baru nut extract was 161 ± 5 μmol trolox equivalent (TE)/L, which was higher than its FRAP, 55 ± 0.5 μmol TE/L. Radical scavenging capacity of baru nut extract was 17 ± 3 and 5 ± 7 μmol TE/L according to DPPH and ORAC, respectively. Conclusions Baru nut is rich in polyphenols and has high antioxidant activity. Further research is warranted to determine the antioxidant properties of baru nut in vitro and in vivo. Funding Sources No sources of funding.

scholarly journals Antioxidant Activities, Phenolic and Flavonoid Contents of Methanolic Extract of Stelechocarpus burahol Fruit and its Fractions

2018 ◽  
Vol 17 (2) ◽  
pp. 153-159
Author(s):  
Nur Herlina ◽  
Sugeng Riyanto ◽  
Sudibyo Martono ◽  
Abdul Rohman
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Diammonium Salt ◽  
Gallic Acid Equivalent ◽  
Phenolic And Flavonoid

Kepel or Stelechocarpus burahol is an exotic fruit originating from Java, Indonesia. This research was intended to evaluate the antioxidant activities of extracts and fractions of kepel fruit pulp (KFP) based on radical scavenging capability towards 2,2′-azinobis(3-ethylbenzo thiazoline-6-sulphonic acid) diammonium salt (ABTS) and 2,2′-diphenildiphenyl-1-picrylhydrazil radical (DPPH). In this study, the radical scavenging activities were also correlated with total phenolics and flavonoid contents. Among the evaluated samples, the ethyl acetate fraction exhibited the highest antiradical antioxidant activity towards ABTS radical with IC50 of 0.35 μg/ml, lower than that of vitamin C (as positive control). It also showed highest antiradical activity using DPPH based assay. Methanolic extract had total phenolic content of 58.28 ± 0.37% wt/wt gallic acid equivalent, higher than its fraction. Meanwhile the petroleum ether soluble fraction revealed flavonoid content (76.06 ± 11.9%) as rutin equivalent, among the extract and fractions evaluated. Based on coefficient (R2) values, phenolics and flavonoids contents contributed to 73.09% and 30.99%% towards antiradical scavenging activities, respectively. Dhaka Univ. J. Pharm. Sci. 17(2): 153-159, 2018 (December)

scholarly journals Antioxidative and Anticholinesterase Activity ofCyphomandra betaceaFruit

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Siti Hawa Ali Hassan ◽  
Mohd Fadzelly Abu Bakar
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Anticholinesterase Activity ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Frap Assay ◽  
Total Anthocyanin ◽  
Gallic Acid Equivalent

Cyphomandra betaceais one of the underutilized fruits which can be found in tropical and subtropical countries. This study was conducted to determine the antioxidant activity and phytochemical contents in different parts (i.e., flesh and peel) of the fruits. Antioxidants were analyzed using DPPH and ABTS free radical scavenging assays as well as FRAP assay. Anticholinesterase activity was determined using enzymatic assay using acetyl cholinesterase enzyme. For 80% methanol extract, the peel of the fruit displayed higher antioxidant activity in both FRAP and ABTS free radical scavenging assays while the flesh displayed higher antioxidant activity in the DPPH assay. Total phenolic and total flavonoid content were higher in the peel with the values of 4.89 ± 0.04 mg gallic acid equivalent (GAE)/g and 3.36 ± 0.01 mg rutin equivalent (RU)/g, respectively. Total anthocyanin and carotenoid content were higher in the flesh of the fruit with the values of 4.15 ± 0.04 mg/100 g and 25.13 ± 0.35 mg/100 g. The anticholinesterase was also higher in the peel ofC. betacea. The same trends of phytochemicals, antioxidant, and anticholinesterase were also observed in the distilled water extracts. These findings suggested thatC. betaceahas a potential as natural antioxidant-rich nutraceutical products.

Sign in / Sign up

Export Citation Format

Share Document