scholarly journals Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Mohsen Keyvanfard ◽  
Khadijeh Alizad ◽  
Razieh Shakeri
Keyword(s):  
Standard Deviation ◽  
Sodium Cromoglycate ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Biological Samples ◽  
Limit Of Detection ◽  
Micellar Medium ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A new kinetic spectrophotometric method is described for the determination of ultratrace amounts of sodium cromoglycate (SCG). The method based on catalytic action of SCG on the oxidation of amaranth with periodate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of the amaranth at 518 nm, for the first 4 min from initiation of the reaction. Calibration curve was linear in the range of 4.0−36.0 ng mL−1SCG. The limit of detection is 2.7 ng mL−1SCG. The relative standard deviation (RSD) for ten replicate analyses of 12, 20, and 28 ng mL−1SCG was 0.40%, 0.32%, and 0.53%, respectively. The proposed method was used for the determination of SCG in biological samples.

scholarly journals The Development of a New Kinetic Spectrophotometric Method for the Determination of Vanadium(V) Based on its Catalytic Effect on the Oxidation of Malachite Green Oxalate by Bromate in Acidic and Micellar Medium

2010 ◽  
Vol 7 (4) ◽  
pp. 1612-1620 ◽  
Author(s):  
M. Keyvanfard ◽  
N. Abedi
Keyword(s):  
Malachite Green ◽  
Spectrophotometric Method ◽  
Catalytic Effect ◽  
Limit Of Detection ◽  
Fixed Time ◽  
Micellar Medium ◽  
Relative Standard ◽  
Kinetic Spectrophotometric ◽  
Malachite Green Oxalate

A new, simple, sensitive and selective kinetic spectrophotometric method was developed for the determination of ultra trace amounts of vanadium(V). The method is based on the catalytic effect of vanadium(V) on the oxidation of malachite green oxalate (MG) by bromate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in the absorbance of malachite green oxalate (MG) at 625 nm with a fixed-time method. The decrease in the absorbance of MG is proportional to the concentration of vanadium(V) in the range of 1-100 ng/mL with a fixed time of 0.5-2 min from the initiation of the reaction. The limit of detection is 0.71 ng/mL of vanadium(V). The relative standard deviation for the determination of 5, 30, 50 ng/mL of vanadium(V) was2.5% 2.6%, 2.4% and respectively. The method was applied to the determination of vanadium(V) in water samples.

Kinetic Spectrophotometric Determination of Ruthenium by its Catalytic Effect on the Reduction of Spadns with Sodium Hypophosphite in Micellar Media

2012 ◽  
Vol 204-208 ◽  
pp. 4067-4070 ◽  
Author(s):  
Zhi Rong Zhou ◽  
Li Zhen Zhang
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Catalytic Effect ◽  
Limit Of Detection ◽  
Fixed Time ◽  
Sodium Hypophosphite ◽  
Micellar Media ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A simple kinetic spectrophotometric method was developed for the determination of trace amounts of Ru (III). The method is based on the reduction of spadns by sodium hypophosphite (NaH2PO2) in micellar media. The reaction was monitored spectrophotometrically by measuring the decrease in the absorbance of spadns at 515 nm with a fixed-time method. The decrease in the absorbance of spadns is proportional to the concentration of Ru (III) in the range 0.40–10.0 μg/L with a fixed time of 2.5–7.0 min from the initiation of the reaction. The limit of detection is 0.12 μg/L Ru (III). The relative standard deviation for the determination of 0.10 and 0.20 μg/25mL Ru (III) was 2.3 % and 2.0 %, respectively. The method was applied to the determination of Ru (III) in some ores and metallurgy products.

scholarly journals Flotation-Spectrophotometric Method for the Determination of Aluminium ion Using Xylenol Orange

2011 ◽  
Vol 8 (4) ◽  
pp. 1528-1535 ◽  
Author(s):  
F. Nekouei ◽  
Sh. Nekouei
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Xylenol Orange ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Ion Associate ◽  
Sensitive Method

A simple, fast, reproducible and sensitive method for the flotation- spectrophotometric determination of Al3+is reported. The apparent molar absorptivity (ε) of the ion associate was determined to be 8.35×104L mol-1cm-1. The calibration curve was linear in the concentration range of 1.0-50 ng mL-1of Al3+with a correlation coefficient of 0.9997. The limit of detection (LOD) was 0.621 ng mL. The relative standard deviation (RSD) at 10 and 30 ng mL-1of aluminium were 1.580 and 2.410% (n=7) respectively. The method was applied for measuring the amount of aluminium in water samples.

Kinetic Spectrophotometric Method for the Determination of Trace Ruthenium(III) by its Catalytic Effect on the Oxidation of Dibromo Caboxy Arsenazo with Bromate in Cationic Micellar Media

2012 ◽  
Vol 602-604 ◽  
pp. 1289-1293 ◽  
Author(s):  
Zhi Rong Zhou ◽  
Li Zhen Zhang
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Catalytic Effect ◽  
Fixed Time ◽  
Micellar Medium ◽  
Micellar Media ◽  
Relative Standard ◽  
Kinetic Spectrophotometric ◽  
Good Agreement

A spectrophotometric method was developed for the determination of trace Ru (III) is described, based on its catalytic effect of Ru (III) on the oxidation of dibromo caboxy arsenazo (DBCAA) by bromate in acidic and micellar medium at 100 °C. The reaction was monitored spectrophotometrically by measuring the decrease in the absorbance of the catalytic reaction of DBCAA at 530 nm with a fixed-time method. The calibration curve for the recommended method was linear in the concentration range over 0.037–1.0 µg/L and the detection limit of the method for Ru (III) was 0.011 µg/L. The method was applied to the determination of Ru (III) in some ores and metallurgy products. The results are in good agreement with the recommend values with the relative standard deviation of 2.6 %–3.8 % and the recovery of 97.2 %–104.3 %.

scholarly journals The Development of a New Inhibition Kinetic Spectrophotometric Method for the Determination of Phenylhydrazine Based on its Inhibitory Effect on Oxidation of Methyl Red by Bromate in Micellar Medium

2010 ◽  
Vol 7 (4) ◽  
pp. 1591-1597
Author(s):  
Mohsen Keyvanfard
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Fixed Time ◽  
Inhibitory Effect ◽  
Methyl Red ◽  
Micellar Medium ◽  
Relative Standard ◽  
Inhibition Kinetic ◽  
Kinetic Spectrophotometric

A new, simple, sensitive and selective kinetic spectrophotometric method was developed for the determination of trace amounts of phenylhydrazine over the range of 0.02-0.30 μg/mL. The method is based on the inhibitory effect of phenylhydrazine on the oxidation of methyl red by bromate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of methyl red at 518 nm with a fixed-time 0.5–2.0 min from initiation of the reaction..The relative standard deviation of 0.08 and 0.2 μg/mL phenylhydrazine was 1.7 and 2.4%, respectively. The method was applied to the determination of phenylhydrazine in water samples.

scholarly journals Spectrophotometric Method for Determination of Paraquat in Food and Biological Samples

1997 ◽  
Vol 80 (2) ◽  
pp. 388-391 ◽  
Author(s):  
Ritu Kesari ◽  
Manish Rai ◽  
Vinay Kumar Gupta
Keyword(s):  
Standard Deviation ◽  
Metal Ions ◽  
Relative Standard Deviation ◽  
Alkaline Medium ◽  
Spectrophotometric Method ◽  
Biological Samples ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

Abstract A sensitive spectrophotometric method was developed for determination of paraquat, a widely used herbicide. Paraquat was reduced with glucose in an alkaline medium, and the blue radical ion obtained was measured at 600 nm. Beer’s law was obeyed at 0.1–1.2 ppm paraquat. The molar absorptivity was 1.26 × 105 L mol-1 cm-1. The standard deviation and relative standard deviation were ± 0.007 and 2.0%, respectively, for 5 μg paraquat/10 mL analyzed over 7 days. The method was free from interference by other commonly used pesticides and metal ions. The method may be used to the determine paraquat in plants, fruits, grains, water, blood, and urine.

scholarly journals Ultraviolet Spectrophotometric Method for Determination of Glipizide in Presence of Liposomal/Proliposomal Turbidity

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Neelkant Prasad ◽  
Roshan Issarani ◽  
Badri Prakash Nagori
Keyword(s):  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Uv Detection ◽  
Limit Of Quantification ◽  
Relative Standard ◽  
Precision And Accuracy ◽  
Clear Solution

A simple and sensitive ultraviolet spectrophotometric method for quantitative estimation of glipizide in presence of lipid turbidity is described to avoid false estimation due to diffraction by turbidity. UV detection was performed at 230 nm, 225 nm, and 235 nm, and the calibration curve was plotted between resultant of absorbance of [230 nm − (225 nm + 235 nm)/2] and concentration of analyte. The calibration curve was linear over the concentration range tested (1–20 μg/mL) with limit of detection of 0.27 μg/mL and limit of quantification of 0.82 μg/mL. Percent relative standard deviations and percent relative mean error, representing precision and accuracy, respectively, for clear as well as turbid solutions, were found to be within acceptable limits, that is, always less than 0.69 and 0.41, respectively, for clear solution and 0.65 and 0.47, respectively, for turbid solution. Conclusively, our method was successfully applied for the determination of glipizide in clear as well as turbid solutions, and it was found that the drug analyte in both types of solutions can be detected from the same calibration curve accurately and precisely and glipizide entrapped in the liposomes or in proliposomal matrix was not detected.

Kinetic Spectrophotometric Method for the Determination of Selenium (IV) by its Catalytic Effect on the Reduction of Arsenazo III with Sodium Hypophosphite in Micellar Media

2012 ◽  
Vol 554-556 ◽  
pp. 926-933
Author(s):  
Zhi Rong Zhou ◽  
Li Zhen Zhang
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Catalytic Effect ◽  
Fixed Time ◽  
Sodium Hypophosphite ◽  
Micellar Media ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A simple and sensitive kinetic spectrophotometric method for the determination of trace selenium (IV) is described, based on its catalytic effect on the reduction arsenazo III (AsA III) with sodium hypophosphite (NaH2PO2) in a solution of 0.02 mol/L sulfuric acid and in the presence of cationic micellar media. The reaction rate is monitored spectrophotometrically by measuring the decrease in absorbance of AsA III at 550 nm with a fixed-time method. The decrease in the absorbance of AsA III is proportional to the concentration of Se(IV) in the range 0.16–1.0 µg/L after a fixed time of 4–10 min from the initiation of the reaction. The limit of detection is 0.049 µg/L Se(IV). The influence of th e factors such as acidity, concentration of reactants, type and concentration of surfactants, reactive time, temperature and co-existing ions on the reaction is discussed. The optimum reaction conditions of reaction are established and some kinetic parameters are determined; the apparent activation energy of catalytic reaction is 59.51 kJ/mol. The relative standard deviation for eleven replicate determination of 0.02, and 0.01µg/25mL Se(IV) was 2.0 % and 2.4 %, respectively. Combined with sulphydryl dextrane gel (SDG) separation and enriching, the method has been successfully applied to the determination of Se (IV) in foodstuff and human hair samples with the relative standard deviation of 2.1 %–5.8 % and the recovery of 97.0 %–103.3 %, the results are in good agreement with those provided by ICP-AES method.

Determination of diltiazem based on the reduction of Cu(II)–BCA complexes in micellar medium

2010 ◽  
Vol 88 (6) ◽  
pp. 533-539 ◽  
Author(s):  
Larissa Zuppardo Lacerda Sabino ◽  
Daniele Cestari Marino ◽  
Horacio Dorigan Moya
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Confidence Level ◽  
Limit Of Detection ◽  
Calibration Graph ◽  
Micellar Medium ◽  
Solution Ph ◽  
Simple Method ◽  
Relative Standard

A simple method was developed for determining microquantities of diltiazem, based on the reduction of copper(II) in buffered solution (pH 7.0) and the use of a micellar medium containing 4,4′-dicarboxy-2,2′-biquinoline acid. The copper(I) produced reacts with 4,4′-dicarboxy-2,2′-biquinoline acid and the complexes formed are spectrophotometrically measured at 558 nm. A typical calibration graph shows good linearity (r = 0.993) from 20 to 100 μg mL–1 of diltiazem. The limit of detection and relative standard deviation were calculated as 12 μg mL–1 (99% confidence level) and 3.5% (40 μg mL–1; n = 6), respectively, with a mean recovery value of 96.5% found in pharmaceutical dosages. A straightforward and effective way to recycle the reagents is addressed. The hazardous aspects of the Cu(I)–BCA reaction are presented as well.

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF CEFPIROME IN PHARMACEUTICAL PREPARATION

2020 ◽  
pp. 124-127
Author(s):  
DILIP M CHAFLE
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Effect Of Temperature ◽  
Percentage Error ◽  
Ferric Nitrate ◽  
Red Color ◽  
Relative Standard

Objective: A simple, sensitive and precise visible spectrophotometric method has been proposed for the determination of cefpirome (CFM) in pure and oral injectable dosage form. Methods: A spectrophotometric method is based on the formation of stable red color product by oxidation of drugs by ferric nitrate and subsequent complexation with 1, 10 – phenanthroline with maximum absorption at 515 nm. Result: The red color complex was formed between Fe (II) and 1, 10 – phenanthroline after reduction of Fe (III) to Fe (II) in the presence of CFM drug. The phosphoric acid solution was used only for quenching the complex formation reaction. Several parameters such as the maximum wavelength of absorption, the volume of reagents, sequence of addition and effect of temperature and time of heating were optimized to achieve high sensitivity, stability and reproducible results. Under the optimum conditions, linear relationship with good correlation coefficient (0.994) was found over the concentration range from 0.20 to 6.00 μg/mL with a molar extinction coefficient 7.7813 × 104 L/mol/cm, limit of detection 0.2026 and limit of quantification 0.6141 μg/mL, respectively. Conclusion: The proposed method was evaluated statistically for linearity, accuracy, and precision in terms of standard deviation, percentage recovery, percentage error and relative standard deviation. The proposed method can be applied for the routine estimation of CFM in the laboratory.

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