Status of Systemic Oxidative Stress during Therapeutic Hypothermia in Patients with Post-Cardiac Arrest Syndrome

Therapeutic hypothermia (TH) is thought to be due to the downregulation of free radical production, although the details of this process remain unclear. Here, we investigate changes in oxidative stress and endogenous biological antioxidant potential during TH in patients with post-cardiac arrest syndrome (PCAS). Nineteen PCAS patients were enrolled in the study. Brain temperature was decreased to the target temperature of 33°C, and it was maintained for 24 h. Patients were rewarmed slowly (0.1°C/h, <1°C/day). The generation of reactive oxygen metabolites (ROMs) was evaluated in plasma samples by d-ROM test. Plasma antioxidant capacity was measured by the biological antioxidant potential (BAP) test. Levels of d-ROMs and BAP levels during the hypothermic stage (33°C) were suppressed significantly compared with pre-TH induction levels (P<0.05), while both d-ROM and BAP levels increased with rewarming (33–36°C) and were correlated with brain temperature. Clinical monitoring of oxidative stress and antioxidant potential is useful for evaluating the redox state of patients undergoing TH after PCAS. Additional therapy to support the antioxidant potential in the rewarming stage following TH may reduce some of the observed side effects associated with the use of TH.

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Effects of Korean black raspberry supplementation on oxidative stress and plasma antioxidant capacity in healthy male smokers

Journal of Functional Foods ◽

10.1016/j.jff.2015.04.047 ◽

2015 ◽

Vol 16 ◽

pp. 393-402 ◽

Cited By ~ 5

Author(s):

Eunkyo Park ◽

SooMuk Cho ◽

Jung eun Lee ◽

Seung Min Lee ◽

Yesl Kim ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Black Raspberry ◽

Healthy Male ◽

Plasma Antioxidant Capacity ◽

Plasma Antioxidant

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Therapeutic hypothermia reduces inflammation and oxidative stress in the liver after asphyxial cardiac arrest in rats

Acute and Critical Care ◽

10.4266/acc.2020.00304 ◽

2020 ◽

Vol 35 (4) ◽

pp. 286-295

Author(s):

Yoonsoo Park ◽

Ji Hyeon Ahn ◽

Tae-Kyeong Lee ◽

Bora Kim ◽

Hyun-Jin Tae ◽

...

Keyword(s):

Oxidative Stress ◽

Cardiac Arrest ◽

Therapeutic Hypothermia ◽

And Oxidative Stress ◽

Asphyxial Cardiac Arrest

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Antioxidant/Oxidant Status and Cardiac Function in Bradykinin B1- and B2-Receptor Null Mice

Physiological Research ◽

10.33549/physiolres.932496 ◽

2013 ◽

pp. 511-517 ◽

Cited By ~ 4

Author(s):

S. DELEMASURE ◽

N. BLAES ◽

C. RICHARD ◽

R. COUTURE ◽

M. BADER ◽

...

Keyword(s):

Oxidative Stress ◽

Free Radical ◽

Cardiovascular Diseases ◽

Vitamin C ◽

Cardiac Function ◽

Cardiac Phenotype ◽

Plasma Antioxidant Capacity ◽

Ascorbyl Free Radical ◽

Plasma Antioxidant ◽

Oxidant Status

Kinin-vasoactive peptides activate two G-protein-coupled receptors (R), B1R (inducible) and B2R (constitutive). Their complex role in cardiovascular diseases could be related to differential actions on oxidative stress. This study investigated impacts of B1R or B2R gene deletion in mice on the cardiac function and plasma antioxidant and oxidant status. Echocardiography-Doppler was performed in B1R (B1R-/-) and B2R (B2R-/-) deficient and wild type (WT) adult male mice. No functional alteration was observed in B2R-/- hearts. B1R-/- mice had significantly lowered fractional shortening and increased isovolumetric contraction time. The diastolic E and A waves velocity ratio was similar in all mice groups. Thus B1R-/- mice provide a model of moderate systolic dysfunction, whereas B2R-/- mice displayed a normal cardiac phenotype. Plasma antioxidant capacity (ORAC) was significantly decreased in both B1R-/- and B2R-/- mice whereas the vitamin C levels were decreased in B2R-/- mice only. Plasma ascorbyl free radical was significantly higher in B1R-/- compared to WT and B2R-/- mice. Therefore, the oxidative stress index, ascorbyl free radical to vitamin C ratio, was increased in both B1R-/- and B2R-/- mice. Hence, B1R and B2R deficiency are associated with increased oxidative stress, but there is a differential imbalance between free radical production and antioxidant defense. The interrelationship between the differential B1R and B2R roles in oxidative stress and cardiovascular diseases remain to be investigated.

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Decreased hepatosplanchnic antioxidant uptake during hepatic ischaemia/reperfusion in patients undergoing liver resection

Clinical Science ◽

10.1042/cs20070317 ◽

2008 ◽

Vol 114 (8) ◽

pp. 553-560 ◽

Cited By ~ 3

Author(s):

Marcel C. G. van de POLL ◽

Cornelis H. C. Dejong ◽

Marc A. J. G. Fischer ◽

Aalt Bast ◽

Ger H. Koek

Keyword(s):

Oxidative Stress ◽

Uric Acid ◽

Liver Resection ◽

Xanthine Oxidase ◽

Oxidase Activity ◽

Ischaemic Preconditioning ◽

Xanthine Oxidase Activity ◽

Ischaemia Reperfusion ◽

Plasma Antioxidant Capacity ◽

Plasma Antioxidant

Oxidative stress mediates cell injury during ischaemia/reperfusion. On the other hand, experimental findings suggest that ROS (reactive oxygen species) induce processes leading to ischaemic preconditioning. The extent and source of oxidative stress and its effect on antioxidant status in the human liver during intermittent ischaemia and reperfusion remains ill-defined. Therefore the aim of the present study was to investigate the occurrence of oxidative stress in humans undergoing liver resection. Liver biopsies, and arterial and hepatic venous blood samples were taken from ten patients undergoing hepatectomy with an intermittent Pringle manoeuvre. Plasma MDA (malondialdehyde) and hepatic GSSG levels were measured as markers of oxidative stress and plasma uric acid as a marker of xanthine oxidase activity. In addition, changes in hepatosplanchnic consumption of plasma antioxidants and hepatic levels of carotenoids and glutathione (GSH) were measured. After ischaemia, hepatosplanchnic release of MDA and increased hepatic GSSG levels were found. This was accompanied by the release of uric acid, reflecting xanthine oxidase activity. During reperfusion, ongoing oxidative stress was observed by further increases in hepatic GSSG content and hepatosplanchnic MDA release. Uric acid release was minimal during reperfusion. A gradual decrease in plasma antioxidant capacity and net hepatosplanchnic antioxidant uptake was observed upon prolonged cumulative ischaemia. Oxidative stress occurs during hepatic ischaemia in man mainly due to xanthine oxidase activity. Interestingly, the gradual decline in plasma antioxidant capacity and net hepatosplanchnic antioxidant uptake during prolonged cumulative ischaemia, preserved both hydrophilic and lipophilic hepatic antioxidant levels. Decreasing plasma levels and net hepatosplanchnic uptake of plasma antioxidants may warrant antioxidant supplementation, although it should be clarified to what extent limitation of oxidative stress compromises ROS-dependent pathways of ischaemic preconditioning.

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Influence of Carbohydrate Ingestion on Oxidative Stress and Plasma Antioxidant Potential Following a 3 h Run

Free Radical Research ◽

10.1080/10715760303850 ◽

2003 ◽

Vol 37 (8) ◽

pp. 1-1

Author(s):

Steven R. M

Keyword(s):

Oxidative Stress ◽

Antioxidant Potential ◽

Carbohydrate Ingestion ◽

Plasma Antioxidant

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Effect of Mixed Flavonoids, n-3 Fatty Acids, and Vitamin C on Oxidative Stress and Antioxidant Capacity Before and After Intense Cycling

International Journal of Sport Nutrition and Exercise Metabolism ◽

10.1123/ijsnem.21.4.328 ◽

2011 ◽

Vol 21 (4) ◽

pp. 328-337 ◽

Cited By ~ 13

Author(s):

Steven R. McAnulty ◽

David C. Nieman ◽

Lisa S. McAnulty ◽

Worley S. Lynch ◽

Fuxia Jin ◽

...

Keyword(s):

Oxidative Stress ◽

Fatty Acids ◽

Vitamin C ◽

Antioxidant Capacity ◽

Repeated Measures ◽

Statistical Design ◽

Potential Health ◽

Plasma Antioxidant Capacity ◽

Plasma Antioxidant ◽

Post Hoc

Consumption of plant flavonoids, antioxidants, and n-3 fatty acids is proposed to have many potential health benefits derived primarily through antioxidant and anti-inflammatory activities. This study examined the effects of 1,000 mg quercetin + 1,000 mg vitamin C (QC); 1,000 mg quercetin, 1,000 mg vitamin C, 400 mg isoquercetin, 30 mg epigallocatechin gallate, and 400 mg n-3 fatty acids (QFO); or placebo (P), taken each day for 2 wk before and during 3 d of cycling at 57% Wmax for 3 hr, on plasma antioxidant capacity (ferricreducing ability of plasma [FRAP], oxygen-radical absorbance capacity [ORAC]), plasma oxidative stress (F2-isoprostanes), and plasma quercetin and vitamin C levels. Thirty-nine athletes were recruited and randomized to QC, QFO, or P. Blood was collected at baseline, after 2 wk supplementation, immediately postexercise, and 14 hr postexercise. Statistical design used a 3 (groups) × 4 (times) repeated-measures ANOVA with post hoc analyses. Plasma quercetin was significantly elevated in QC and QFO compared with P. Plasma F2-isoprostanes, FRAP, and vitamin C were significantly elevated and ORAC significantly decreased immediately postexercise, but no difference was noted in the overall pattern of change. Post hoc analyses revealed that the QC and QFO groups did not exhibit a significant increase in F2-isoprostanes from baseline to immediately postexercise compared with P. This study indicates that combining flavonoids and antioxidants with n-3 fatty acids is effective in reducing the immediate postexercise increase in F2-isoprostanes. Moreover, this effect occurs independently of changes in plasma antioxidant capacity.

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