scholarly journals Effect of Mixed Flavonoids, n-3 Fatty Acids, and Vitamin C on Oxidative Stress and Antioxidant Capacity Before and After Intense Cycling

2011 ◽  
Vol 21 (4) ◽  
pp. 328-337 ◽  
Author(s):  
Steven R. McAnulty ◽  
David C. Nieman ◽  
Lisa S. McAnulty ◽  
Worley S. Lynch ◽  
Fuxia Jin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Repeated Measures ◽  
Statistical Design ◽  
Potential Health ◽  
Plasma Antioxidant Capacity ◽  
Plasma Antioxidant ◽  
Post Hoc

Consumption of plant flavonoids, antioxidants, and n-3 fatty acids is proposed to have many potential health benefits derived primarily through antioxidant and anti-inflammatory activities. This study examined the effects of 1,000 mg quercetin + 1,000 mg vitamin C (QC); 1,000 mg quercetin, 1,000 mg vitamin C, 400 mg isoquercetin, 30 mg epigallocatechin gallate, and 400 mg n-3 fatty acids (QFO); or placebo (P), taken each day for 2 wk before and during 3 d of cycling at 57% Wmax for 3 hr, on plasma antioxidant capacity (ferricreducing ability of plasma [FRAP], oxygen-radical absorbance capacity [ORAC]), plasma oxidative stress (F2-isoprostanes), and plasma quercetin and vitamin C levels. Thirty-nine athletes were recruited and randomized to QC, QFO, or P. Blood was collected at baseline, after 2 wk supplementation, immediately postexercise, and 14 hr postexercise. Statistical design used a 3 (groups) × 4 (times) repeated-measures ANOVA with post hoc analyses. Plasma quercetin was significantly elevated in QC and QFO compared with P. Plasma F2-isoprostanes, FRAP, and vitamin C were significantly elevated and ORAC significantly decreased immediately postexercise, but no difference was noted in the overall pattern of change. Post hoc analyses revealed that the QC and QFO groups did not exhibit a significant increase in F2-isoprostanes from baseline to immediately postexercise compared with P. This study indicates that combining flavonoids and antioxidants with n-3 fatty acids is effective in reducing the immediate postexercise increase in F2-isoprostanes. Moreover, this effect occurs independently of changes in plasma antioxidant capacity.

scholarly journals Antioxidant/Oxidant Status and Cardiac Function in Bradykinin B1- and B2-Receptor Null Mice

2013 ◽  
pp. 511-517 ◽  
Author(s):  
S. DELEMASURE ◽  
N. BLAES ◽  
C. RICHARD ◽  
R. COUTURE ◽  
M. BADER ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Cardiovascular Diseases ◽  
Vitamin C ◽  
Cardiac Function ◽  
Cardiac Phenotype ◽  
Plasma Antioxidant Capacity ◽  
Ascorbyl Free Radical ◽  
Plasma Antioxidant ◽  
Oxidant Status

Kinin-vasoactive peptides activate two G-protein-coupled receptors (R), B1R (inducible) and B2R (constitutive). Their complex role in cardiovascular diseases could be related to differential actions on oxidative stress. This study investigated impacts of B1R or B2R gene deletion in mice on the cardiac function and plasma antioxidant and oxidant status. Echocardiography-Doppler was performed in B1R (B1R-/-) and B2R (B2R-/-) deficient and wild type (WT) adult male mice. No functional alteration was observed in B2R-/- hearts. B1R-/- mice had significantly lowered fractional shortening and increased isovolumetric contraction time. The diastolic E and A waves velocity ratio was similar in all mice groups. Thus B1R-/- mice provide a model of moderate systolic dysfunction, whereas B2R-/- mice displayed a normal cardiac phenotype. Plasma antioxidant capacity (ORAC) was significantly decreased in both B1R-/- and B2R-/- mice whereas the vitamin C levels were decreased in B2R-/- mice only. Plasma ascorbyl free radical was significantly higher in B1R-/- compared to WT and B2R-/- mice. Therefore, the oxidative stress index, ascorbyl free radical to vitamin C ratio, was increased in both B1R-/- and B2R-/- mice. Hence, B1R and B2R deficiency are associated with increased oxidative stress, but there is a differential imbalance between free radical production and antioxidant defense. The interrelationship between the differential B1R and B2R roles in oxidative stress and cardiovascular diseases remain to be investigated.

Effect of pharmacological lowering of plasma urate on exercise-induced oxidative stress

2007 ◽  
Vol 32 (6) ◽  
pp. 1148-1155 ◽  
Author(s):  
S. R. McAnulty ◽  
P. A. Hosick ◽  
L. S. McAnulty ◽  
J. C. Quindry ◽  
L. Still ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Repeated Measures ◽  
Lipid Hydroperoxides ◽  
Double Blind ◽  
Reducing Ability ◽  
Plasma Antioxidant Capacity ◽  
Plasma Urate ◽  
Exercise Induced ◽  
The Impact

Urate is a metabolic end product of purine metabolism that contributes about 66% of the antioxidant capacity of plasma. The objective of this study was to evaluate the importance of plasma urate as an antioxidant using pharmacological lowering and examining the impact on plasma antioxidant capacity and oxidative stress after intense exercise. Fifteen subjects ran for 45 min at ~80% VO2 max under the influence of probenecid (1 g/d) (PRO) or placebo (PLA) in a double-blind, crossover design. Blood samples obtained at baseline, pre-exercise, and immediately post-exercise were analyzed for F2-isoprostanes, lipid hydroperoxides (LHs), ferric-reducing ability of plasma (FRAP), urate, ascorbate (AA), and nitrite. A 2 (group) × 2 (time) repeated-measures analysis of variance (ANOVA), one-way ANOVA, Tukey–Kramer multiple comparison tests, and Student’s t tests were used for statistical analysis. PRO exhibited lowered urate and FRAP compared with baseline (p ≤ 0.05), and group effects existed for the exercise trials (p = 0.023 and p ≤ 0.001, respectively) versus PLA. F2-isoprostanes, nitrite, and AA were increased after exercise (p = 0.004, p = 0.001, and p = 0.003, respectively), but the pattern of change was not different between treatments. This study indicates that plasma markers of exercise-induced oxidative stress were not affected by below-normal physiological concentrations of urate and a diminished antioxidant capacity within the plasma compartment.

scholarly journals EFFECT OF MANGO (Mangifera indica) CV. AZÚCAR JUICE CONSUMPTION ON PLASMA ANTIOXIDANT CAPACITY AND OXIDATIVE STRESS BIOMARKERS

Revista Vitae ◽  
2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Maria bibiana Zapata ◽  
Ana Ramos Polo ◽  
Andres Felipe Alzate ◽  
Luis Fernando Restrepo Betancur ◽  
Benjamin Rojano ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Mangifera Indica ◽  
Total Phenolic ◽  
Healthy Individuals ◽  
Total Glutathione ◽  
Plasma Antioxidant Capacity ◽  
Plasma Antioxidant

Background: Mango (Mangifera indica L.) is one of the world’s most consumed fruit, and it is also a rich source of antioxidants that may prevent oxidative stress. Objectives: This study aimed to determine if mango (cv. Azúcar) juice can improve the antioxidant status of healthy individuals with low consumption of vegetables and fruit. Methods: This was a cross-over single-blind study carried out with 16 healthy individuals for 73 days. Participants were randomly assigned to either a mango juice period or a placebo period. Total phenolic content, antioxidant capacity, mangiferin, thiobarbituric acid reactive substances (TBARS), total glutathione, and 8-hydroxydeoxyguanosine levels were determined in plasma. Results: Plasma antioxidant activity was significantly higher in the juice consumption period than the placebo consumption period; however, total phenolic content, total glutathione, TBARS, and 8-hydroxydeoxyguanosine levels did not show significant differences between juice period and placebo period. Mangiferin was detected in every participant after juice consumption. Conclusions: Mango (cv. Azúcar) juice daily consumption improves plasma antioxidant capacity.

scholarly journals Effects of Dietary Strawberry Supplementation on Antioxidant Biomarkers in Obese Adults with Above Optimal Serum Lipids

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Arpita Basu ◽  
Stacy Morris ◽  
Angel Nguyen ◽  
Nancy M. Betts ◽  
Dongxu Fu ◽  
...  
Keyword(s):  
Trace Elements ◽  
Enzyme Activity ◽  
Antioxidant Capacity ◽  
Serum Lipids ◽  
Low Dose ◽  
High Dose ◽  
Obese Adults ◽  
Plasma Antioxidant Capacity ◽  
Dose Control ◽  
Plasma Antioxidant

Berries have shown several cardiovascular health benefits and have been associated with antioxidant functions in experimental models. Clinical studies are limited. We examined the antioxidant effects of freeze-dried strawberries (FDS) in adults [n=60; age:49±10years; BMI:36±5 kg/m2(mean ± SD)] with abdominal adiposity and elevated serum lipids. Participants were randomized to one of the following arms: low dose strawberry (25 g/day FDS), low dose control beverage (LD-C), high dose strawberry (50 g/d FDS), and high dose control beverage (HD-C) for 12 weeks. Control beverages were matched for calories and total fiber. Plasma antioxidant capacity, trace elements (copper, iron, selenium, and zinc), whole blood glutathione (GSH), and enzyme activity (catalase, glutathione peroxidase, and glutathione reductase) were examined at screening (0 week) and after 12 weeks’ intervention. At 12 weeks, plasma antioxidant capacity and glutathione levels were higher in the strawberry versus control groups (low and high dose FDS: 45% and 42% for plasma antioxidant capacity and 28% and 36% for glutathione, resp.); glutathione was higher in the high versus low dose strawberry group (allp<0.05). Serum catalase activity was higher in the low dose strawberry (43%) versus control group (p<0.01). No differences were noted in plasma trace elements and glutathione enzyme activity. Dietary strawberries may selectively increase plasma antioxidant biomarkers in obese adults with elevated lipids.

Plasma Antioxidant Capacity Of Olympic Sailors Prior To The Olympic Games

2009 ◽  
Vol 41 ◽  
pp. 2
Author(s):  
Nathan A. Lewis ◽  
Brian Moore ◽  
Pete Cunningham ◽  
Lindy Castell ◽  
Jan Knight
Keyword(s):  
Antioxidant Capacity ◽  
Olympic Games ◽  
Plasma Antioxidant Capacity ◽  
Plasma Antioxidant ◽  
The Olympic Games

scholarly journals Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 87
Author(s):  
Francesca Perut ◽  
Laura Roncuzzi ◽  
Sofia Avnet ◽  
Annamaria Massa ◽  
Nicoletta Zini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Mammalian Cells ◽  
Dependent Manner ◽  
Potential Health ◽  
Human Mesenchymal Stromal Cells ◽  
Health Promoting ◽  
Size And Morphology

Plant-derived exosome-like nanovesicles (EPDENs) have recently been isolated and evaluated as potential bioactive nutraceutical biomolecules. It has been hypothesized that EPDENs may exert their activity on mammalian cells through their specific cargo. In this study, we isolated and purified EPDENs from the strawberry juice of Fragaria x ananassa (cv. Romina), a new cultivar characterized by a high content of anthocyanins, folic acid, flavonols, and vitamin C and an elevated antioxidant capacity. Fragaria-derived EPDENs were purified by a series of centrifugation and filtration steps. EPDENs showed size and morphology similar to mammalian extracellular nanovesicles. The internalization of Fragaria-derived EPDENs by human mesenchymal stromal cells (MSCs) did not negatively affect their viability, and the pretreatment of MSCs with Fragaria-derived EPDENs prevented oxidative stress in a dose-dependent manner. This is possibly due to the presence of vitamin C inside the nanovesicle membrane. The analysis of EPDEN cargo also revealed the presence of small RNAs and miRNAs. These findings suggest that Fragaria-derived EPDENs may be considered nanoshuttles contained in food, with potential health-promoting activity.

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