scholarly journals Phenotypic Profile of Rh and Kell Blood Group Systems among Blood Donors in Cote d’Ivoire, West Africa

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
L. Siransy Bogui ◽  
B. Dembele ◽  
Y. Sekongo ◽  
S. Abisse ◽  
S. Konaté ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Sub Saharan Africa ◽  
Blood Group Antigens ◽  
Red Cell ◽  
Phenotypic Profile ◽  
Blood Transfusion Center ◽  
Donor Population ◽  
Sub Saharan ◽  
Design And Methods

Few countries in sub-Saharan Africa make systematic searches for antigens C, c, E, and e of the Rh and Kell system antigens in the donor and recipient, thereby exposing transfused patients. Purpose and Objectives. In this paper, we propose to determine the red cell Rh and Kell blood groups among blood donors from traditional techniques to improve medical care of transfused patients. This study will allow us to assess the frequency of blood group antigens in these systems. Study Design and Methods. We carried out a study on the red cell typing in the blood donor population of the National Blood Transfusion Center in Abidjan. This study was performed on 651 blood donors. Results. For the Rh system, the antigen frequencies of D, c, e, C, and E are, respectively, 92.93%, 99.85%, 99.85%, 21.97%, and 13.82%. K antigen is found in 0.77% of donors. Discussion and Conclusion. Although the frequencies of the most immunogenic antigens are lower than in the white race, lack of preventive measures makes the immunological risk high in Africa. Furthermore, Africa is full of specificities that are important to note for a better care of our patients.

scholarly journals PREVALENCE OF BLOOD GROUP ANTIGENS OTHER THAN ABO-RhD IN HEALTHY BLOOD DONORS AT A TERTIARY CARE CENTER IN WESTERN RAJASTHAN, INDIA

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Ravindra Kumar Yadav ◽  
Dev Raj Arya ◽  
N.L. Mahawar ◽  
Arun Bharti ◽  
Shailendra Vashistha ◽  
...  
Keyword(s):  
Blood Group ◽  
Analytical Study ◽  
Blood Donors ◽  
Care Center ◽  
Tertiary Care ◽  
Blood Group Antigens ◽  
Tertiary Care Center ◽  
Cross Sectional ◽  
Donor Population ◽  
Compatible Blood

Introduction: It is important to know the frequencies of the various antigens to predict the availability of blood units for alloimmunized patients. Because of the fact that there is always a chance of diversity in phenotype pattern of a donor population, we decided to conduct a study on antigen phenotyping of regular blood donors. Methodology: This blood bank based cross-sectional analytical study was carried out amongst 500 voluntary blood donors over a period of 8 months, i.e., from April, 2019 to November, 2019. Samples from all these donors were subjected to extended phenotyping (C, c, E, e, K, M, N, S, Jka, Jkb, Fya, Fyb, Lea and Leb). Results: In present study, we observed the percentage frequencies of C, c, E, e, K, M, N, S, P1, Lea, Leb, Jka, Jkb, Fya and Fyb antigens as 75.6%, 53.2%, 18.4%, 97.75%, 3.8%, 82.4%, 58.4%, 43.8%, 66.2%, 16.8%, 52.6%, 80.0%, 67.6%, 79.4% and 54.6% respectively. Conclusion: Outcomes of such studies can be used to formulate a rare blood group donor registry and compatible blood can be provided to the patients (especially those requiring multiple transfusions). Keywords: Antigens, Phenotyping, Blood donors.

scholarly journals Distribution of RH and Kell (K) blood group antigens among blood donors in a tertiary care hospital of Jammu region, India

2019 ◽  
Vol 7 (4) ◽  
pp. 1308 ◽  
Author(s):  
Irm Yasmeen ◽  
Meena Sidhu ◽  
Ibrar Ahmed
Keyword(s):  
Ethnic Groups ◽  
Blood Group ◽  
Blood Donors ◽  
Tertiary Care ◽  
Data Bank ◽  
Blood Groups ◽  
Blood Group Antigens ◽  
Red Cell ◽  
Cell Antigen ◽  
K Antigen

Background: Knowledge about the frequency of red cell antigen phenotype is very important for the creation of donor data bank and to minimize the risk of alloimmunization. This requires the determination of immunological characteristics of blood products and blood recipients by performing phenotyping of clinically significant blood group antigens. The aims and objectives were to study the distribution of Rh and Kell (K) antigen among blood donors of different ethnic groups in a tertiary care hospital.Methods: This was prospective observational cross sectional one-point analysis study which was carried out over a period of one year with effect from November 2015 to October 2016 in the Postgraduate Department of Immunohematology and Blood Transfusion Medicine, Shri Maharaja Gulab Singh (SMGS) Hospital, Government Medical College, Jammu and Kashmir, India. It comprised of voluntary and replacement donors and categorized into different ethnic groups i.e Dogras, Gujjar Muslims, Non-Gujjar Muslims, Kashmiri Pandits, Sikhs and Christian. Donors selection criteria was as per Drug and Cosmetic Act.Results: A total of 500 (Five hundred) blood samples from the donors of all blood groups were typed for the presence of Rh (D, C, E, c, e) and Kell (K) antigens. Out of these 500 samples, 420 were antigen typed by conventional tube technique and 80 samples were typed by column agglutination technique using glass beads. As per ethnicity, maximum donors were Dogras (74%) followed by Non-Gujjar Muslims (9.4%), Gujjar Muslims (9%), Sikhs (5.6%), Kashmiri Pandits (1.4%) and Christians were the least in frequency (0.6%). On phenotyping for Rh and Kell antigens ‘e’ antigen have the ubiquitous distribution and was found to have the highest frequency 486 (97.2%) followed by ‘D’ antigen 472 (94.4%), ‘C’ antigen 426 (85.2%), ‘c’ antigen 320 (64.0%) and ‘E’ antigen 103 (20.6%). Overall frequency of Kell (K) antigen was 2.6%.Conclusions: Knowledge of red cell antigen phenotype frequencies in a population with different ethnic groups can help in creating donor data bank and database for the distribution of blood groups for preparing inhouse cell panels and providing proper antigen compatible blood for patients with multiple alloantibodies and also reduce the risk of RBC antigen alloimmunization along with their complications.

scholarly journals Detection of red cell alloantibodies in thalassaemia patients

2020 ◽  
Vol 7 (2) ◽  
pp. 419 ◽  
Author(s):  
Ansuman Sahu ◽  
Pankaj Parida ◽  
Smita Mahapatra ◽  
Binay Bhusan Sahoo
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Red Cells ◽  
P Value ◽  
Blood Group Antigens ◽  
Red Cell ◽  
Thalassaemia Major ◽  
Medical College ◽  
Blood Group Serology ◽  
Clinically Significant

Background: β-thalassaemia patients receive regular blood transfusion to thrive. Due to antigen disparity between the blood donors and these patients they develop red cell alloantibodies due to alloimmunization.  The objective of this study is to predict the frequency of red cell alloimmunization amongst β-thalassaemia major patients receiving regular blood transfusion.Methods: This study including 106 patients with β-thalassaemia was conducted in the department of Transfusion Medicine, S. C. B. Medical College, Cuttack for a period of 12 months. Alloantibodies to different red cell blood group antigens in multi-transfused thalassaemia patients were detected using the glass bead technology for blood group serology in the present study.Results: Out of 106 β-thalassaemia major patients included in the study, 7.5% of patients developed alloantibodies, all being clinically significant. The alloantibodies were anti-E, anti c, anti e and anti-D. The rate of incidence of these alloantibodies was 3.8%, 1.9%, 0.9% and 0.9% respectively.  There was a significant association between alloantibody formation with number of transfused packed red cells (Mann-Whitney Test: p value = 0.035) and age at first transfusion (p value = 0.001). The factors having no association with alloimmunization to red cell antigens are age and gender.Conclusions: Alloimmunization to various erythrocyte blood group antigens is a common problem in multi-transfused β-thalassaemia patients. There is an association between number of transfused packed red cells and age at first transfusion with alloantibody formation in the study.

scholarly journals ABO, Rhesus blood groups and transfusion-transmitted infections among blood donors in Gabon

2018 ◽  
Vol 13 (1) ◽  
pp. 12 ◽  
Author(s):  
Christ-Dominique Ngassaki-Yoka ◽  
Jophrette Mireille Ntsame Ndong ◽  
Cyrille Bisseye
Keyword(s):  
Blood Group ◽  
Blood Donor ◽  
Representative Sample ◽  
Blood Donors ◽  
Blood Groups ◽  
Abo Blood Group ◽  
Blood Group Antigens ◽  
Abo Blood Groups ◽  
Donor Population ◽  
Monospecific Antisera

Background: Few studies focused on the study of blood groups in Gabon. This study aimed to determine the phenotypic frequency of ABO and Rhesus antigens in blood donors of Libreville and to assess the association between ABO blood groups and transfusion-transmitted infections.Materials and Methods: The study of ABO and Rhesus blood groups concerned 4,744 blood donors. ABO and Rhesus phenotyping were obtained using monoclonal monospecific antisera: anti-A, anti-B, anti-AB, anti-D, anti-E, anti-C, anti-c, and anti-e with an automate (QWALYS® 3, DIAGAST, France) or a card gel (ID Card, BIO-RAD) according to manufacturer’s instructions.Results: The phenotypic frequency of blood group antigens A, B, AB and O were respectively 21.0%; 17.6%; 2.6% and 58.9%. Those of rhesus antigens D, d, C, c, E and e were 97.7%; 2.3%; 15.9%; 99.9%; 17.6%; 99.3%, respectively. The prevalence of ABO and Rh antigens in Gabonese donors reported here are significantly different from those of neighboring countries. No association was found between the prevalence of HIV, HCV and syphilis and ABO blood groups. Instead, HBV seroprevalence was twice as high among non-O blood groups donors compared with blood group O donors [OR = 2 (CI 1.26 to 3.2), p = 0.003].Conclusions: This study provides new data on phenotypic frequency of ABO and Rh blood groups in a representative sample of the Gabonese blood donor population. It suggests a significant association between ABO blood group and HBV infection.

scholarly journals Prevalence of Red Blood Cell Major Blood Group Antigens and Phenotypes among Omani Blood Donors

2019 ◽  
Vol 34 (6) ◽  
pp. 496-503
Author(s):  
Arwa Z. Al-Riyami ◽  
Ali Al-Marhoobi ◽  
Saif Al-Hosni ◽  
Sabah Al Mahrooqi ◽  
Michael Schmidt ◽  
...  
Keyword(s):  
Blood Cell ◽  
Blood Group ◽  
Red Blood Cell ◽  
Blood Donors ◽  
Blood Group Antigens

Prevalence of Duffy Blood Group Antigens and Phenotypes among Saudi Blood Donors in Southwestern Saudi Arabia

2021 ◽  
Vol 67 (01/2021) ◽  
Author(s):  
Amr Halawani ◽  
Muhammad Saboor ◽  
Hisham Abu-Tawil ◽  
Ayman Mahzari ◽  
Abdullah Mansor ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Blood Group ◽  
Blood Donors ◽  
Blood Group Antigens ◽  
Duffy Blood Group

The risk of hepatitis B virus infection by transfusion in Kumasi, Ghana

Blood ◽  
2003 ◽  
Vol 101 (6) ◽  
pp. 2419-2425 ◽  
Author(s):  
Jean-Pierre Allain ◽  
Daniel Candotti ◽  
Kate Soldan ◽  
Francis Sarkodie ◽  
Bruce Phelps ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Hbv Dna ◽  
Hbv Infection ◽  
Sub Saharan Africa ◽  
B Virus ◽  
Sub Saharan ◽  
Hbv Transmission

The risk of hepatitis B virus (HBV) transmission by transfusion in sub-Saharan Africa is considered to be relatively low, and testing of blood donors is often not done or is done relatively poorly. To re-examine this attitude, we identified HBV chronically infected blood donors from a major hospital in Ghana with a range of hepatitis B surface antigen (HBsAg) assays. Test efficacy was estimated using HBV DNA as a gold standard, and the risk of HBV infection in blood recipients was estimated for different testing strategies. Particle agglutination, dipstick, and enzyme immunoassay (EIA) HBsAg screening detected 54%, 71%, and 97% of HBV infectious donors, respectively. The risk of HBV transmission to recipients less than 10 years old ranged between 1:11 and 1:326 with blood unscreened and screened by EIA, respectively. For older recipients, the risk decreased a further 4-fold because of the high frequency of natural exposure to HBV. A total of 98% of HBsAg-confirmed positive samples contained HBV DNA. HBV DNA load was less than 1 × 104 IU/mL in 75% of HBsAg-reactive samples, most of them anti-HBe reactive. Approximately 0.5% of HBsAg-negative but anti-HBc-positive samples contained HBV DNA. The use of sensitive HBsAg tests is critical to prevent transfusion transmission of HBV infection to young children in a population with a 15% prevalence of chronic HBV infection in blood donors. However, this will not have much effect on the prevalence of this infection unless other strategies to protect children from infection are also advanced in parallel.

scholarly journals Use of Domain-Deletion Mutants to Locate Lutheran Blood Group Antigens to Each of the Five Immunoglobulin Superfamily Domains of the Lutheran Glycoprotein: Elucidation of the Molecular Basis of the Lua/Lub and the Aua/Aub Polymorphisms

Blood ◽  
1997 ◽  
Vol 89 (11) ◽  
pp. 4219-4225 ◽  
Author(s):  
S.F. Parsons ◽  
G. Mallinson ◽  
G.L. Daniels ◽  
C.A. Green ◽  
J.S. Smythe ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
K562 Cells ◽  
Base Change ◽  
Immunoglobulin Superfamily ◽  
Deletion Mutants ◽  
Blood Group Antigens ◽  
Reading Frame ◽  
Polymerase Chain ◽  
Domain 3

Abstract Lutheran glycoprotein (Lu gp) has five predicted immunoglobulin superfamily (IgSF ) domains. K562 cells were transfected with Lu cDNA and tested by flow cytometry with monoclonal antibodies and Lu blood group antisera. The results confirmed the identity of Lu cDNA. Deletion mutants lacking the regions encoding one or more IgSF domains were made by inverse polymerase chain reaction (PCR), expressed in K562 cells, and tested with the same antibodies. The Lub and Lu5 antigens and the epitope recognized by monoclonal antibody BRIC 224 were mapped to the first, N-terminal, IgSF domain. Lu4 and Lu8 were mapped to domain 2; Lu20 to domain 3; Lu7 and BRIC 221 epitope to domain 4, and Lu13 and Aub to domain 5. The organization of the LU gene was determined. The region encoding the open reading frame is arranged in 15 exons extending over ≈11 kb on chromosome 19q13.2. The Lua/Lub and Aua/Aub blood group polymorphisms were studied using genomic DNA from typed blood donors. The Lua mutation is a base change in exon 3 (G252 to A) encoding an Arg77 (Lub) to His (Lua) change on the CFG face of domain 1. The Aua/Aub polymorphism is an A1637 to G substitution in exon 12 encoding a Thr539 (Aua) to Ala (Aub) change on the G strand of domain 5.

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