The Frequency of the Wr^a, By and Ms Blood Group Antigens in Blood Donors in the South of England

Abstract Lutheran glycoprotein (Lu gp) has five predicted immunoglobulin superfamily (IgSF ) domains. K562 cells were transfected with Lu cDNA and tested by flow cytometry with monoclonal antibodies and Lu blood group antisera. The results confirmed the identity of Lu cDNA. Deletion mutants lacking the regions encoding one or more IgSF domains were made by inverse polymerase chain reaction (PCR), expressed in K562 cells, and tested with the same antibodies. The Lub and Lu5 antigens and the epitope recognized by monoclonal antibody BRIC 224 were mapped to the first, N-terminal, IgSF domain. Lu4 and Lu8 were mapped to domain 2; Lu20 to domain 3; Lu7 and BRIC 221 epitope to domain 4, and Lu13 and Aub to domain 5. The organization of the LU gene was determined. The region encoding the open reading frame is arranged in 15 exons extending over ≈11 kb on chromosome 19q13.2. The Lua/Lub and Aua/Aub blood group polymorphisms were studied using genomic DNA from typed blood donors. The Lua mutation is a base change in exon 3 (G252 to A) encoding an Arg77 (Lub) to His (Lua) change on the CFG face of domain 1. The Aua/Aub polymorphism is an A1637 to G substitution in exon 12 encoding a Thr539 (Aua) to Ala (Aub) change on the G strand of domain 5.

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Prevalence of ABO, RhD and other clinically significant Blood Group Antigens among blood donors at tertiary care center, Gwalior

Bali Medical Journal ◽

10.15562/bmj.v9i2.1779 ◽

2020 ◽

Vol 9 (2) ◽

pp. 437

Author(s):

Shelendra Sharma ◽

Dharmesh Chandra Sharma ◽

Sunita Rai ◽

Anita Arya ◽

Reena Jain ◽

...

Keyword(s):

Blood Group ◽

Blood Donors ◽

Care Center ◽

Tertiary Care ◽

Blood Group Antigens ◽

Tertiary Care Center ◽

Clinically Significant

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Molecular genotyping of Indian blood group antigens amongst regular voluntary blood donors of Surat city, Gujarat, India

Transfusion and Apheresis Science ◽

10.1016/j.transci.2021.103325 ◽

2021 ◽

pp. 103325

Author(s):

Avani Shah ◽

Parizad Patel ◽

Keyuri Jariwala ◽

Farzin Qureshi ◽

Kanchan Mishra ◽

...

Keyword(s):

Blood Group ◽

Blood Donors ◽

Blood Group Antigens ◽

Molecular Genotyping ◽

Indian Blood

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Frequency of Minor Blood Group Antigens Among Hispanic-American Blood Donors in Southwest Texas.

Blood ◽

10.1182/blood.v114.22.4188.4188 ◽

2009 ◽

Vol 114 (22) ◽

pp. 4188-4188

Author(s):

Michael Silvey ◽

Rachel Beddard ◽

Yidong Chen ◽

Melissa Frei-Jones

Keyword(s):

Blood Group ◽

Race And Ethnicity ◽

Retrospective Cohort ◽

Blood Donors ◽

Conflicts Of Interest ◽

South Texas ◽

Blood Type ◽

Blood Group Antigens ◽

Hispanic American ◽

Antigen Testing

Abstract Abstract 4188 The frequency of minor blood group antigens has not been described among Hispanic blood donors. A retrospective cohort study was performed using the South Texas Blood and Tissue Center (STBTC) donor database, LifeTrak. Blood donors self-identified race and ethnicity. Caucasian and Hispanic donors were eligible. Donors who did not have minor antigen testing performed were not included. The STBTC has a high proportion of Hispanic blood donors (299047/779409, 38%), who are primarily of Mexican ancestry. Minor antigen testing was performed in 10% (74140/779409) of all donors; 8% (25394/299407) of Hispanic donors and 11% (42891/392074) of Caucasian donors. Hispanic donors were significantly more like to express blood type O Rh+ than Caucasian donors (13920/25394, 55% v 14947/74140, 35%; p<0.001). The frequency of ABO and RhD blood groups were similar to those reported previously in Caucasian and Hispanic donors (Garraty, et al. Transfusion, 2004). Of the minor antigens, expression of Rh E also differed significantly between Hispanic and Caucasian donors (4706/11744, 40% v 5416/19497, 28%; p<0.001). The prevalence of the other minor antigens were more similar and are reported in Figure 1. Complete Rh haplotypes were available in 8296 Caucasian donors and 7176 Hispanic donors. The haplotype RhDce differed significantly in 36% of Caucasians and 50% of Hispanics (3022/8296 v 3589/7176). The haplotype RhDce was previously reported as most common in African-Americans at 44% (Avent, et al. Blood, 2000). Haplotype RhDCE was found in 8% of Caucasians and 10% of Hispanics (660/8296 v 643/7176); haplotype RhDcE in 16% of Caucasians and 15% of Hispanics (1354/8296 v 1041/7176); and haplotype RhDCe was found in 39% of Caucasians and 27% of Hispanics (3260/8296 v 1903/7176). Differences exist in the frequency of minor blood group antigens among Caucasian and Hispanic blood donors in Southwest Texas. Disclosures: No relevant conflicts of interest to declare.

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SNP Genotyping and LD Testing in ERMAP: Revealing Scianna Blood Group Diversity in NIH Blood Donors

Blood ◽

10.1182/blood.v118.21.2322.2322 ◽

2011 ◽

Vol 118 (21) ◽

pp. 2322-2322

Author(s):

Courtney A. Follit ◽

Patricia A. R. Brunker ◽

Willy A. Flegel

Keyword(s):

Native American ◽

Ethnic Groups ◽

Blood Group ◽

Blood Donors ◽

Rare Variants ◽

Leader Sequence ◽

Blood Group System ◽

Blood Group Antigens ◽

Group System ◽

Transfusion Reactions

Abstract Abstract 2322 Background: The Scianna blood group system has been implicated in cases of hemolytic disease of the fetus and newborn and the detection of antibodies to rare antigens in this system have impacted on transfusion management in some patients. Recently, it has been discovered that the Scianna blood group antigens are expressed by the erythrocyte membrane-associated protein (ERMAP), a 475 amino acid red cell adhesion protein consisting of 12 exons with the transcription region spanning exons 3–12. Rare variants in exons 4 and 12 have been reported in patients who have made antibodies to Scianna antigens or have a serological null phenotype for the Scianna system. ERMAP is a member of the butyrophilin-like family, featuring an extracellular immunoglobulin variable and intracellular B30.2 domains. Although one ERMAP variant is detected in one commercial molecular assay (Sc1/Sc2), most reported variants in this gene are rare, and therefore remain largely unrecognized during transfusion planning. ERMAP polymorphisms remain unreported on a large scale, contributing to the uncertainty concerning their clinical significance. To fill this void, we characterized seventeen single nucleotide polymorphisms (SNPs) in exons 3, 4, and 12 of ERMAP in 905 repeat blood donors. Methods: The DNA of consenting, repeat NIH blood donors were genotyped for seventeen variants in the ERMAP gene. DNA was isolated from whole blood using the Qiagen's MagAttract EZ1 kit. Following polymerase chain reaction amplification, the samples were genotyped by ligation detection reaction (LDR). LDR utilizes a thermostable ligase to generate single stranded DNA fragments of engineered length with allele-specific fluorescent labels, allowing for rapid, multiplexed genotyping. Ligated products were resolved by capillary electrophoresis (3730 DNA analyzer and GeneMapper software (Life Technologies)). Results: Eleven of the seventeen variants (G35S, R81Q, nt307Δ2, Q296Q, R332X, R392H, L399L, L409L, S442P, L452P, and L452L) were monomorphic in this cohort (N=905). Overall, the 54c>t and 76c>t transitions in exon 3 had minor allele frequencies (MAF) of 0.21 and 0.23, respectively, and appeared in all self-identified ethnic groups (except Native American donors (n=2)) with maxima observed in donors of self-identified Hispanic ethnicity (n=16; MAF=0.41 and 0.44, respectively). These SNPs showed significant linkage disequilibrium (r2=0.86 [95%CI 0.85–0.88]). African-American donors (n=57) had the highest frequency of variant 11c>t (MAF 0.07) and variant 755c>t (MAF 0.018), which was absent or extremely rare in other ethnic groups. The Caucasian donor population was the only group to display variations 788g>a and 1094g>a (MAF 0.003 and 0.0008 respectively). Conclusions: This is the largest sample of blood donors to be comprehensively genotyped for Scianna variants to date. We observed population-specific polymorphism of these rare variants according to the donor's self-identified ethnicity, which is under further study. Determining the diversity in the Scianna blood group system may help explain otherwise unclear transfusion reactions, particularly if these variants impact on Scianna antigen surface density (especially the predicted leader sequence variants in exon 3) or other ERMAP functions (via variants in the intracellular domain encoded by exon 12). High throughput donor genotyping will allow evaluation of the clinical importance in alloimmunization for variants like the 11c>t, 54c>t, and 76c>t SNPs that lie in the predicted leader sequence and polymorphisms 755c>t, 788g>a, and 1094g>a that lie within the intracellular B30.2 domain of the ERMAP protein. Awareness of the frequencies of these variations can therefore be a clinically useful aid in the investigation of donors implicated in transfusion reactions. Disclosures: No relevant conflicts of interest to declare.

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Prevalence of Principal Rh Blood Group Antigens in Blood Donors at the Blood Bank of a Tertiary Care Hospital in Southern India

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2016/16621.7726 ◽

2016 ◽

Cited By ~ 2

Author(s):

Deepthi Krishna Gundrajukuppam

Keyword(s):

Blood Group ◽

Blood Donors ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Blood Bank ◽

Southern India ◽

Blood Group Antigens ◽

Care Hospital ◽

Rh Blood Group

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PREVALENCE OF BLOOD GROUP ANTIGENS OTHER THAN ABO-RhD IN HEALTHY BLOOD DONORS AT A TERTIARY CARE CENTER IN WESTERN RAJASTHAN, INDIA

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v4i3.1028 ◽

2020 ◽

Vol 4 (3) ◽

Author(s):

Ravindra Kumar Yadav ◽

Dev Raj Arya ◽

N.L. Mahawar ◽

Arun Bharti ◽

Shailendra Vashistha ◽

...

Keyword(s):

Blood Group ◽

Analytical Study ◽

Blood Donors ◽

Care Center ◽

Tertiary Care ◽

Blood Group Antigens ◽

Tertiary Care Center ◽

Cross Sectional ◽

Donor Population ◽

Compatible Blood

Introduction: It is important to know the frequencies of the various antigens to predict the availability of blood units for alloimmunized patients. Because of the fact that there is always a chance of diversity in phenotype pattern of a donor population, we decided to conduct a study on antigen phenotyping of regular blood donors. Methodology: This blood bank based cross-sectional analytical study was carried out amongst 500 voluntary blood donors over a period of 8 months, i.e., from April, 2019 to November, 2019. Samples from all these donors were subjected to extended phenotyping (C, c, E, e, K, M, N, S, Jka, Jkb, Fya, Fyb, Lea and Leb). Results: In present study, we observed the percentage frequencies of C, c, E, e, K, M, N, S, P1, Lea, Leb, Jka, Jkb, Fya and Fyb antigens as 75.6%, 53.2%, 18.4%, 97.75%, 3.8%, 82.4%, 58.4%, 43.8%, 66.2%, 16.8%, 52.6%, 80.0%, 67.6%, 79.4% and 54.6% respectively. Conclusion: Outcomes of such studies can be used to formulate a rare blood group donor registry and compatible blood can be provided to the patients (especially those requiring multiple transfusions). Keywords: Antigens, Phenotyping, Blood donors.

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