Prevalence of Duffy Blood Group Antigens and Phenotypes among Saudi Blood Donors in Southwestern Saudi Arabia

BACKGROUND: The development of periodontal diseases depends on the presence of causative microorganisms, host immunity and risk factors. Although variability present among the types of periodontal diseases, all are represented to a shared interaction between host and bacteria. ABO blood groups are the most investigated erythrocyte antigen system. However, limited investigations have been conducted to explore the alliance between ABO blood groups and periodontal diseases. AIM: Our purpose was to explore any possible association between the severity of chronic periodontitis with ABO blood groups and Rh factor. METHODS: A cross-sectional study was carried out on 205 patients out of 1126 generalised chronic periodontitis patients (GCP) who were referred to Al-Farabi Colleges, Riyadh, Saudi Arabia. They were categorized into; group I (mild), group II (moderate) and group III (sever). RESULTS: The patients with blood group O were at a greater risk to develop GCP irrespective of its severity, followed by those with blood group A, B, and AB. The dispensation of the Rh factor in all groups exhibited a significantly greater distribution of Rh positive. CONCLUSION: Genetic factors such as ABO blood group antigens may act as a risk influencer that affects the progression and severity of the chronic periodontitis.

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Prevalence of Red Blood Cell Major Blood Group Antigens and Phenotypes among Omani Blood Donors

Oman Medical Journal ◽

10.5001/omj.2019.92 ◽

2019 ◽

Vol 34 (6) ◽

pp. 496-503

Author(s):

Arwa Z. Al-Riyami ◽

Ali Al-Marhoobi ◽

Saif Al-Hosni ◽

Sabah Al Mahrooqi ◽

Michael Schmidt ◽

...

Keyword(s):

Blood Cell ◽

Blood Group ◽

Red Blood Cell ◽

Blood Donors ◽

Blood Group Antigens

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The Frequency of the Wr^a, By and Ms Blood Group Antigens in Blood Donors in the South of England

Vox Sanguinis ◽

10.1159/000478608 ◽

1960 ◽

Vol 5 (6) ◽

pp. 556-560

Author(s):

T.E. Gleghorn

Keyword(s):

Blood Group ◽

Blood Donors ◽

Blood Group Antigens ◽

The South

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Use of Domain-Deletion Mutants to Locate Lutheran Blood Group Antigens to Each of the Five Immunoglobulin Superfamily Domains of the Lutheran Glycoprotein: Elucidation of the Molecular Basis of the Lua/Lub and the Aua/Aub Polymorphisms

Blood ◽

10.1182/blood.v89.11.4219 ◽

1997 ◽

Vol 89 (11) ◽

pp. 4219-4225 ◽

Cited By ~ 47

Author(s):

S.F. Parsons ◽

G. Mallinson ◽

G.L. Daniels ◽

C.A. Green ◽

J.S. Smythe ◽

...

Keyword(s):

Blood Group ◽

Blood Donors ◽

K562 Cells ◽

Base Change ◽

Immunoglobulin Superfamily ◽

Deletion Mutants ◽

Blood Group Antigens ◽

Reading Frame ◽

Polymerase Chain ◽

Domain 3

Abstract Lutheran glycoprotein (Lu gp) has five predicted immunoglobulin superfamily (IgSF ) domains. K562 cells were transfected with Lu cDNA and tested by flow cytometry with monoclonal antibodies and Lu blood group antisera. The results confirmed the identity of Lu cDNA. Deletion mutants lacking the regions encoding one or more IgSF domains were made by inverse polymerase chain reaction (PCR), expressed in K562 cells, and tested with the same antibodies. The Lub and Lu5 antigens and the epitope recognized by monoclonal antibody BRIC 224 were mapped to the first, N-terminal, IgSF domain. Lu4 and Lu8 were mapped to domain 2; Lu20 to domain 3; Lu7 and BRIC 221 epitope to domain 4, and Lu13 and Aub to domain 5. The organization of the LU gene was determined. The region encoding the open reading frame is arranged in 15 exons extending over ≈11 kb on chromosome 19q13.2. The Lua/Lub and Aua/Aub blood group polymorphisms were studied using genomic DNA from typed blood donors. The Lua mutation is a base change in exon 3 (G252 to A) encoding an Arg77 (Lub) to His (Lua) change on the CFG face of domain 1. The Aua/Aub polymorphism is an A1637 to G substitution in exon 12 encoding a Thr539 (Aua) to Ala (Aub) change on the G strand of domain 5.

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The Presence of FYAnull Alele of Duffy Blood Group System in Blood Donors and Individuals from a Malarial Endemic Region of Brazil.

Blood ◽

10.1182/blood.v104.11.2714.2714 ◽

2004 ◽

Vol 104 (11) ◽

pp. 2714-2714 ◽

Cited By ~ 2

Author(s):

Dante M. Langhi ◽

Sergio R. Albuquerque ◽

Dimas T. Covas ◽

Clovis A. Perez ◽

Jose O. Bordin

Keyword(s):

Sequence Analysis ◽

Blood Group ◽

Blood Donors ◽

Single Point Mutation ◽

Binding Motif ◽

Blood Group System ◽

Endemic Region ◽

Group System ◽

Duffy Blood Group ◽

Gata Box

Abstract BACKGROUND: Malaria is a virulent disease caused by the Plasmodium parasite. Innate resistance to malaria infections in humans is conferred by various blood group polymorphisms. The Duffy blood group system consists of Fya and Fyb antigens which are encoded by codominant alleles FYA and FYB. Four phenotypes are defined: Fy(a+b+), Fy(a+b−), Fy(a−b+) and Fy(a−b−). Erythrocytes of Duffy-negative individuals are resistant to invasion by P. vivax. In Blacks the Fy(a−b−) phenotype is associated with a single point mutation (-33T-C) in the GATA-1 binding motif for the erythroid promoter of FYB. STUDY DESIGN AND METHODS: We investigated the phenotypes and the genotypes of Duffy blood group system of 250 individuals living in a malarial endemic region (MER) in the state of Amazon (Brazil), and of 199 blood donors (BD) from a non-endemic region. The phenotyping was done by agglutination gel tests (DiaMed-Latino América) using anti-Fya and anti-Fyb reagents. The molecular analysis for FYA, FYB, FYBES (GATA box mutation nt -33T-C), and FYBWeak (mutations 265 C-T, and 298 G-A) alleles, were performed by PCR-RFLP. The PCR products were digested by Ban I for FYA and FYB identifications; by Sty I for GATA box mutation; Acy I and Mwo I for 265 C-T and 298 G-A mutations, respectively. Some samples that showed discrepancy between the phenotype and genotype results were examined by sequence analysis using the ABI PrismâBig Dyeä Terminator Cycle Sequencing Ready Reaction Kit” (Perkin Elmer), and the interpretation by the software ABI PRISMä 377 DNA Sequencer”, 3.3 version (Perkin Elmer). RESULTS: We found that 34/250 (13.6%) of 250 persons living in the MER and 37/199 (18.6%) of BD had phenotype and genotype discrepant results [Fy(a+b−) FYA/FYB]. In addition, we found that 16/34 (47%) of people living in the MER, and 4/37 (10.8%) of BD did not present the -33T-C mutation, the 265 C-T, or the 298 G-A mutations. The sequence analysis of 2 samples from persons from MER indicated the presence of -33T-C mutation in the FYA allele in one individual (1 FYA/FYB and W/M; FYA/FYB and M/M). Additionally, we detected that 18/34 (53%) of people living in the MER, and 33/37 (89.2%) of BD presented the -33T-C mutation. The sequence analysis of 5 samples indicated the presence of -33T-C mutation in the FYA allele in 4 cases [2 persons from MER and 2 from BD (FYA/FYB e M/M)]. CONCLUSION: Recently the mutation responsible for erythrocyte Duffy antigen-negativity [Fy(a−b−)] was demonstrated in FYA allele in a malarial endemic region of Papua New Guinea. The present data demonstrated the presence of the FYAnull allele not only in persons living in a malarial endemic region but also in Brazilian blood donors from non-endemic areas. In contrast with that which happens with the FYB allele, our results indicated that the presence of the -33T-C mutation in the FYA allele does not abolish the expression of the Fya antigen in the erythrocyte.

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Detection of antibodies reacting with the antithetical duffy blood group antigens Fya and Fyb using recombinant fusion proteins containing the duffy extracellular domain

Transfusion and Apheresis Science ◽

10.1016/j.transci.2006.09.003 ◽

2006 ◽

Vol 35 (3) ◽

pp. 207-216 ◽

Cited By ~ 3

Author(s):

William P. Sheffield ◽

Varsha Bhakta ◽

Donald R. Branch ◽

Gregory A. Denomme

Keyword(s):

Blood Group ◽

Fusion Proteins ◽

Extracellular Domain ◽

Blood Group Antigens ◽

Duffy Blood Group ◽

Recombinant Fusion Proteins

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Prevalence of ABO, Rh and KELL Blood Group Types and Transfusion- Transmissible Infections (TTI) among Blood Donors in Najran City, Saudi Arabia

Biomedical & Pharmacology Journal ◽

10.13005/bpj/2210 ◽

2021 ◽

Vol 14 (02) ◽

pp. 1065-1076

Author(s):

Osama M Alshehri ◽

Mohammed H Nahari ◽

Elhashimi E Hassan ◽

Musab F Alqahtani ◽

Turki H Awaji

Keyword(s):

Saudi Arabia ◽

Blood Transfusion ◽

Hepatitis B ◽

Blood Group ◽

Blood Donation ◽

Complete Blood Count ◽

Blood Groups ◽

Type I ◽

Blood Group Antigens ◽

Safe Blood

The knowledge of Red blood cells polymorphism and blood group antigens prevalence at the local and regional levels is necessary for safe blood transfusion services. This study was aimed to estimate the prevalence of significant blood group phenotypes like ABO, Rh, and Kell among the Najran people of Saudi Arabia. The transfusion transmittable infection (TTI) rate and blood abnormalities among various blood types were assessed to ensure safe blood transfusion. ABO and Rh blood prevalence (n=970) and Rh phenotype polymorphism were estimated in over 531 unrelated donors. The blood samples were screened for certain TTIs like AHBC- Anti-hepatitis B core, HTLV-1- human T-lymphotropic virus type I, HCV- hepatitis C virus, HBsAg- Hepatitis B antigen, HIV- Human immunodeficiency virus, SIC- Sickle cell, MP- Malaria parasite, and SYP- Syphilis. The selected samples were also observed for blood abnormalities by performing a complete blood count (CBC). Out of 970 subjects, 966 were males, and only 4 were females. The O>A>B>AB blood groups were identified with 46.89, 29.3, 9.1, and 2.38% prevalence among Rh-positive phenotype. While in the Rh system, 87.6% and 12.3% of Rh positive and Rh negative was observed. Among 953 samples, the prevalence of seropositive donors was approximately 5.66%. The screening showed about 5.036, 0.104, 0.314, 0.209, 2.18, 0.104, and 0.209% positivity for AHBC, HCV, HBsAg, HIV, SIC, MP, and SYP respectively. Results found that the frequency of D, C, E, c, and e were 99.9%, 67.98, 25.8, 77.9, 98.49%, respectively, in over 531 subjects. The e allele was more prevalent in Najran city. After observing the variations in the CBC parameters among the donors, it was perceived that about 28.78, 99.9, 29.41, and 31.6% of blood abnormalities were noticed for O, AB, B, and A blood groups, respectively. For blood banks and transfusion services, which play a significant role in the medical care of the patient, awareness of the distribution of the blood group is essential. Increasing consistency of blood donation programs would improve both donor satisfaction and motivation for potential donations of blood in near future.

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