Protective Effects of Tualang Honey against Oxidative Stress and Anxiety-Like Behaviour in Stressed Ovariectomized Rats

The present study aims to evaluate the antioxidant and anxiolytic-like effect of Tualang honey in stressed ovariectomized (OVX) rats. The animals were divided into; (i) nonstressed sham-operated control rats, (ii) sham-operated control rats exposed to stress, (iii) nonstressed OVX rats, (iv) OVX rats exposed to stress, (v) OVX rats exposed to stress and treated with 17 β-oestradiol (E2) (20 μg daily, sc), and (vi) OVX rats exposed to stress and treated with Tualang honey (0.2 g/kg body weight, orally). The open field test was used to evaluate the anxiety-like behaviour and ELISA kits were used to measure oxidant/antioxidant status of the brain homogenates. The result showed that anxiety-like behavior was significantly increased in stressed OVX compared to other groups, and administering either E2 or Tualang honey significantly decreased anxiety-like behaviour in stressed OVX rats. The levels of malondialdehyde (MDA) and protein carbonyl (PCO) were significantly decreased while the levels/activities of superoxide dismutase (SOD), glutathione S-transferases (GST), glutathione peroxidase (GPx), and glutathione reductase (GR) were significantly increased in the brain homogenates of treated stressed OVX groups compared to untreated stressed OVX. In conclusion, Tualang honey has protective effects against brain oxidative stress and may be useful alternative anxiolytic agent especially for postmenopausal women.

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Tualang Honey Attenuates Noise Stress-Induced Memory Deficits in Aged Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/1549158 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 10

Author(s):

Khairunnuur Fairuz Azman ◽

Rahimah Zakaria ◽

Che Badariah Abdul Aziz ◽

Zahiruddin Othman

Keyword(s):

Oxidative Stress ◽

Memory Performance ◽

Acetylcholinesterase Activity ◽

Protein Carbonyl ◽

Protective Effects ◽

Aged Rats ◽

Stress Exposure ◽

Object Recognition Test ◽

Noise Stress ◽

Tualang Honey

Ageing and stress exposure may lead to memory impairment while oxidative stress is thought to be one of the underlying mechanisms involved. This study aimed to investigate the potential protective effects of Tualang honey supplementation on memory performance in aged rats exposed to noise stress. Tualang honey supplementation was given orally, 200 mg/kg body weight for 28 days. Rats in the stress group were subjected to loud noise, 100 dB(A), 4 hours daily for 14 days. All rats were subjected to novel object recognition test for evaluation of memory performance. It was observed that the rats subjected to noise stress exhibited significantly lower memory performance and higher oxidative stress as evident by elevated malondialdehyde and protein carbonyl levels and reduction of antioxidant enzymes activities compared to the nonstressed rats. Tualang honey supplementation was able to improve memory performance, decrease oxidative stress levels, increase brain-derived neurotrophic factor (BDNF) concentration, decrease acetylcholinesterase activity, and enhance neuronal proliferation in the medial prefrontal cortex (mPFC) and hippocampus. In conclusion, Tualang honey protects against memory decline due to stress exposure and/or ageing via enhancement of mPFC and hippocampal morphology possibly secondary to reduction in brain oxidative stress and/or upregulation of BDNF concentration and cholinergic system.

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Estrogen Replacement Reduces Oxidative Stress in the Rostral Ventrolateral Medulla of Ovariectomized Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/2158971 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 14

Author(s):

Fan Hao ◽

Ying Gu ◽

Xing Tan ◽

Yu Deng ◽

Zhao-Tang Wu ◽

...

Keyword(s):

Oxidative Stress ◽

Protein Expression ◽

Rostral Ventrolateral Medulla ◽

Sympathetic Tone ◽

Ovariectomized Rats ◽

Ventrolateral Medulla ◽

Sympathetic Outflow ◽

Protective Effects ◽

Estrogen Replacement ◽

Ovx Rats

Cardiovascular disease prevalence rises rapidly after menopause, which is believed to be derived from the loss of estrogen. It is reported that sympathetic tone is increased in postmenopause. The high level of oxidative stress in the rostral ventrolateral medulla (RVLM) contributes to increased sympathetic outflow. The focus of this study was to determine if estrogen replacement reduces oxidative stress in the RVLM and sympathetic outflow in the ovariectomized (OVX) rats. The data of this study showed that OVX rat increased oxidative stress in the RVLM and sympathetic tone; estrogen replacement improved cardiovascular functions but also reduced the level of oxidative stress in the RVLM. These findings suggest that estrogen replacement decreases blood pressure and sympathoexcitation in the OVX rats, which may be associated with suppression in oxidative stress in the RVLM through downregulation of protein expression of NADPHase (NOX4) and upregulation of protein expression of SOD1. The data from this study is beneficial for our understanding of the mechanism of estrogen exerting cardiovascular protective effects on postmenopause.

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Genistein reduced the neural apoptosis in the brain of ovariectomised rats by modulating mitochondrial oxidative stress

British Journal Of Nutrition ◽

10.1017/s0007114510002291 ◽

2010 ◽

Vol 104 (9) ◽

pp. 1297-1303 ◽

Cited By ~ 39

Author(s):

Yan-Hong Huang ◽

Qing-Hong Zhang

Keyword(s):

Oxidative Stress ◽

Learning And Memory ◽

Caspase 3 ◽

Visual Learning ◽

Morris Water Maze Test ◽

High Dose ◽

Dependent Manner ◽

Ovx Rats ◽

Neural Apoptosis ◽

The Brain

The present study was undertaken to investigate the antioxidant effect of chronic ingestion of genistein (Gen) against neural death in the brain of ovariectomised (Ovx) rats. The rats were randomly divided into five groups, i.e. sham-operated (sham), Ovx-only, Ovx with 17β-oestradiol, Ovx with low (15 mg/kg) and high (30 mg/kg) doses of Gen (Gen-L and Gen-H), and were orally administered daily with drugs or vehicle for 6 weeks. The learning and memory abilities were measured by Morris water maze test. Oxidative damages in the brain were evaluated by the level of superoxide dismutase (SOD), malondialdehyde (MDA) and monoamine oxidase (MAO) activities. Neural apoptosis was shown by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining and caspase-3 activity. In the visual learning and memory test, there were no significant differences among the population means of the five groups. While in the probe trial test, the Gen-L group instead of the Gen-H group exhibited reduced escape latency and increased memory frequency than the Ovx group. Although both doses of Gen could reduce acetylcholinesterase activity, only a low dose of Gen could diminish MDA activity significantly in frontal cortex and enhance SOD content in the hippocampus. In contrast, MAO content was decreased in the cortex by either dose of Gen, while in the hippocampus, only a high dose of Gen appeared to be effective. Interestingly, Gen at both the doses could attenuate the increased number of TUNEL-positive neurons and caspase-3 activity in Ovx rats. These results suggest that Gen confers protection against Ovx-induced neurodegeneration by attenuating oxidative stress, lipid peroxidation and the mitochondria-mediated apoptotic pathway in a region- and dose-dependent manner.

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TUALANG HONEY ATTENUATES KAINIC ACID-INDUCED OXIDATIVE STRESS IN RAT CEREBELLUM AND BRAINSTEM

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i12.21084 ◽

2017 ◽

Vol 9 (12) ◽

pp. 155 ◽

Cited By ~ 2

Author(s):

Nur Shafika Mohd Sairazi ◽

K. N. S. Sirajudeen ◽

Mustapha Muzaimi ◽

Mummedy Swamy ◽

Mohd Asnizam Asari ◽

...

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Total Antioxidant Status ◽

Thiobarbituric Acid ◽

Protein Carbonyl ◽

Multiple Time ◽

Rat Cerebellum ◽

Total Antioxidant ◽

Multiple Time Points ◽

Tualang Honey

Objective: The present study examined the protective effect of tualang honey (TH) against kainic acid (KA)-induced oxidative stress in the cerebellum and brainstem of rats.Methods: Male Sprague-Dawley rats were randomly divided into four groups: Control, KA-treated, TH+KA-treated, and topiramate (TPM, an antiepileptic agent)+KA-treated groups. Rats were pretreated orally with drinking water, TH (1.0 g/kg body weight), or TPM (40 mg/kg body weight), respectively, five times at 12 h intervals. Saline or KA (15 mg/kg body weight) were injected subcutaneously 30 min after last oral treatment. Rats were sacrificed at 2 h, 24 h, and 48 h after KA administration. Oxidative stress markers were analyzed in different brain regions (cerebellum and brainstem) 2 h, 24 h, and 48 h after KA administration.Results: KA caused significant (p<0.05) elevation in the thiobarbituric acid reactive substances level, protein carbonyl contents, and nitric oxide production, impairment of glutathione system, and a significant reduction in the total antioxidant status in the rat cerebellum and brainstem at multiple time-points, as compared to control groups. Pretreatment with TH significantly (p<0.05) reduced the elevation in the thiobarbituric acid reactive substances level, protein carbonyl contents, and nitric oxide production and increasing a reduction in the total antioxidant status in the rat cerebellum and brainstem induced by KA at multiple time-points, as compared to KA only-treated group.Conclusion: Taken together, this study suggests that TH has therapeutic potential in reducing oxidative stress in the cerebellum and brainstem of KA-induced rats via its antioxidant property.

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Effect of Propofol and Fentanyl on Brain Oxidative Stress after Systemic Lipopolysaccharide Injection in Rats

Reactive Oxygen Species ◽

10.20455/ros.2021.r.801 ◽

2021 ◽

Vol 11 ◽

Author(s):

Omar M.E. Abdel-Salam ◽

Eman R. Youness ◽

Nadia A. Mohammed ◽

Amr M.M. Ibrahim

Keyword(s):

Oxidative Stress ◽

Active Form ◽

Saline Group ◽

Paraoxonase 1 ◽

Stress Markers ◽

Anesthetic Agents ◽

Systemic Endotoxemia ◽

Brain Oxidative Stress ◽

The Brain ◽

Different Doses

Systemic inflammation causes brain oxidative stress, a prerequisite for neurodegeneration. In this study, we investigated the effect of the anesthetic agents propofol and fentanyl on brain oxidative stress during mild systemic endotoxemia induced by lipopolysaccharide (LPS) endotoxin. For this purpose, rats were administered LPS (400 μg/kg, intraperitoneally; i.p.), treated at the same time with different doses of propofol or fentanyl, i.p., and euthanized 4 h later. Other groups were treated with the saline, only propofol, or only fentanyl. Oxidative stress markers including malondialdehyde (MDA), nitric oxide (NO), and reduced glutathione (GSH) were determined. In addition, nuclear factor kappaB (NF-kB), paraoxonase-1 (PON-1), and butyrylcholinesterase (BChE) activities were measured in the brain tissue. Results showed that compared with the saline group, administration of LPS caused a marked and significant increase in brain MDA and NO combined with depletion of GSH and decreased PON-1 and BChE activities. Additionally, the active form of NF-kB was significantly increased in the brain of LPS only-treated rats. Treatment with propofol or fentanyl led to a marked and significant decrease in the levels of brain MDA and NO together with a significant increase in GSH and restoration of PON-1 and BChE activities. Furthermore, lower levels of active form of NF-kB were found following treatment with propofol or fentanyl compared with those in the LPS only group. Collectively, these results suggest that propofol and fentanyl exhibit an antioxidant action and attenuate the endotoxin-induced brain oxidative stress.

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Reversal of age-associated oxidative stress in mice by PFT, a novel kefir product

International Journal of Immunopathology and Pharmacology ◽

10.1177/2058738420950149 ◽

2020 ◽

Vol 34 ◽

pp. 205873842095014

Author(s):

Mamdooh Ghoneum ◽

Shaymaa Abdulmalek ◽

Deyu Pan

Keyword(s):

Oxidative Stress ◽

Low Density Lipoprotein ◽

Redox Homeostasis ◽

Density Lipoprotein ◽

Antioxidant Enzyme Activities ◽

Protective Effects ◽

Oxidative Stress Biomarkers ◽

Age Related ◽

Total Antioxidant ◽

The Brain

Introduction: Oxidative stress is a key contributor to aging and age-related diseases. In the present study, we examine the protective effects of PFT, a novel kefir product, against age-associated oxidative stress using aged (10-month-old) mice. Methods: Mice were treated with PFT orally at a daily dose of 2 mg/kg body weight over 6 weeks, and antioxidant status, protein oxidation, and lipid peroxidation were studied in the brain, liver, and blood. Results: PFT supplementation significantly reduced the oxidative stress biomarkers malondialdehyde (MDA) and nitric oxide; reversed the reductions in glutathione (GSH) levels, total antioxidant capacity (TAC), and anti-hydroxyl radical (AHR) content; enhanced the antioxidant enzyme activities of glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD); inhibited the liver enzyme levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT); significantly reduced triglyceride (TG), total cholesterol (TC), and low density lipoprotein (LDL) levels; and significantly elevated high density lipoprotein (HDL) levels. Interestingly, PFT supplementation reversed the oxidative changes associated with aging, thus bringing levels to within the limits of the young control mice in the brain, liver, and blood. We also note that PFT affects the redox homeostasis of young mice and that it is corrected post-treatment with PFT. Conclusion: Our findings show the effectiveness of dietary PFT supplementation in modulating age-associated oxidative stress in mice and motivate further studies of PFT’s effects in reducing age-associated disorders where free radicals and oxidative stress are the major cause.

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Valsartan Reduces Left Ventricular Hypertrophy in Ovariectomized Spontaneous Hypertensive Rats

American Journal of Hypertension ◽

10.1093/ajh/hpz186 ◽

2020 ◽

Vol 33 (4) ◽

pp. 371-371

Author(s):

Hong Ding ◽

Ning-ying Li ◽

Xiang Zhang ◽

Pan-pan Zhang ◽

Jing Yu

Keyword(s):

Oxidative Stress ◽

Wall Thickness ◽

Interventricular Septum ◽

Posterior Wall ◽

Left Ventricular ◽

Ovariectomized Rats ◽

Control Group ◽

Hypertensive Rats ◽

Sham Control ◽

Ovx Rats

Abstract Objective To investigate the effects of valsartan on left ventricular mass, function, and oxidative stress in ovariectomized spontaneous hypertensive rats (SHR). Methods Twelve-week-old female SHRs were randomly divided into ovariectomy (OVX) control (n = 12), OVX + valsartan (n = 12), sham control (Sham, n = 13), and Sham + valsartan (n = 14) groups. Valsartan (30 mg/kg/day) or double-distilled water was given by oral gavage. After 12 weeks of valsartan or water treatment, left ventricular wall thickness and function, superoxide dismutase (SOD), glutathione peroxidase (GSH), and 8-hydroxydeoxyguanosine (8-OHdG) were assessed. Results There was a significant interaction between ovariectomy and valsartan on interventricular end-diastolic septum thickness (IVSTd), end-systolic interventricular septum thickness (IVSTs), left ventricular end-diastolic posterior wall thickness (LVPWTd), and left ventricular end diastolic diameter (LVEDD) (P < 0.05). Valsartan treatment in OVX rats decreased IVSTd, IVSTs, LVPWTd, and LVPWTs compared to OVX control (P < 0.05). Compared with Sham + control group, LVESP and ±dP/dt of LV were decreased while LVEDP was increased in OVX + control group (all P < 0.05). After valsartan treatment, LVESP and ±dP/dt of LV were increased and LVEDP was decreased in ovariectomized rats (all P < 0.05). Ovariectomy decreased GSH and SOD levels and increased 8-OHdG levels, which were reversed by valsartan treatment (all P < 0.05). Conclusion Valsartan treatment decreases oxidative stress, reduces LV hypertrophy, and improves cardiac function in overiectomized SHR.

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