Differentiation of Human Breast-Milk Stem Cells to Neural Stem Cells and Neurons

Objectives.Human breast milk contains a heterogeneous population of cells that have the potential to provide a noninvasive source of cells for cell therapy in many neurodegenerative diseases without any ethical concern. The objectives of this study were to differentiate the breast milk-derived stem cells (BMDSC) toward neural stem cells and then into the neurons and neuroglia.Materials and Methods.To do this, the BMDSC were isolated from human breast milk and cultured in Dulbecco’s modified Eagle medium/F12 (DMEM/F12) containing fibroblast growth factor (bFGF). The cells were then characterized by evaluation of the embryonic and stem cell markers. Then, the cells were exposed to culture medium containing 1% B27 and 2% N2 for 7–10 days followed by medium supplemented with B27, N2, bFGF 10 µg/mL, and endothelial growth factor (EGF) 20 µg/mL. Then, the sphere-forming assay was performed. The spheres were then differentiated into three neural lineages by withdrawing growth factor in the presence of 5% FBS (fetal bovine serum). The immunofluorescence was done forβ-tubulin III, O4, and GFAP (glial fibrillary acidic protein).Results.The results indicated that the cells expressed both embryonic and mesenchymal stem cell (MSC) markers. They also showed neurospheres formation that was nestin-positive. The cells were also differentiated into all three neural lineages.Conclusion.The BMDSC can behave in the same way with neural stem cells. They were differentiated into oligodendrocytes, and astrocytes as well as neurons.

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Reduction of Vascular Endothelial Growth Factor A in Human Breast Milk After Intravitreal Injection of Bevacizumab but Not Ranibizumab

Archives of Ophthalmology ◽

10.1001/archophthalmol.2012.112 ◽

2012 ◽

Vol 130 (9) ◽

pp. 1226 ◽

Cited By ~ 10

Author(s):

Christoph Ehlken ◽

Gottfried Martin ◽

Andreas Stahl ◽

Hansjürgen Thomas Agostini

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Breast Milk ◽

Intravitreal Injection ◽

Human Breast Milk ◽

Vascular Endothelial ◽

Human Breast ◽

Endothelial Growth Factor

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Vascular Endothelial Growth Factor (VEGF) Is Present in Human Breast Milk and Its Receptor Is Present on Intestinal Epithelial Cells

Pediatric Research ◽

10.1203/00006450-199905010-00007 ◽

1999 ◽

Vol 45 ◽

pp. 652-657 ◽

Cited By ~ 56

Author(s):

Constantinos G Siafakas ◽

Fani Anatolitou ◽

Robert D Fusunyan ◽

W Allan Walker ◽

Ian R Sanderson

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Breast Milk ◽

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Human Breast Milk ◽

Vascular Endothelial ◽

Intestinal Epithelial ◽

Human Breast ◽

Endothelial Growth Factor

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Soluble Vascular Endothelial Growth Factor Receptor 1 in Human Breast Milk

Hormone Research in Paediatrics ◽

10.1159/000324057 ◽

2011 ◽

Vol 76 (1) ◽

pp. 17-21 ◽

Cited By ~ 3

Author(s):

Taner Ozgurtas ◽

Ibrahim Aydin ◽

Ozden Turan ◽

Esin Koc ◽

Ibrahim M. Hirfanoglu ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Breast Milk ◽

Growth Factor Receptor ◽

Human Breast Milk ◽

Vascular Endothelial ◽

Human Breast ◽

Endothelial Growth Factor

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Breast Milk Stem Cell Survival in Neonate's Gut, Entery into Neonate Circulation and Adaption by the Body

Current Stem Cell Research & Therapy ◽

10.2174/1574888x14666191107095728 ◽

2020 ◽

Vol 15 (2) ◽

pp. 98-101 ◽

Cited By ~ 1

Author(s):

Amit Ghosh

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Breast Milk ◽

Systemic Circulation ◽

The Body ◽

Future Research ◽

Human Breast Milk ◽

Human Breast ◽

Cell Therapies ◽

Therapeutic Uses

: The stem cell exchange during pregnancy is thought to remain chimeras for life. Few studies recently revealed that maternal transfer of viable stem cells to the offspring continues even after birth during breastfeeding. Some of these stem cells are likely to be integrated into different organs (brain, blood, kidneys, and pancreas) including neurons and insulin-producing cells in the pancreas to become functional cells. This finding opens a new avenue for research on therapeutic uses of breast milk- derived stem cells. : Recently Dr. Foteini Hassiotou used glowing mice, which were genetically modified to express a gene called tdTomato that causes cells to appear red under fluorescence light. These mice were mated, and their babies were swapped with the pups of another, unmodified mother mouse. The new pups suckled the modified mouse and, as a result, obtained glowing red stem cells from breast milk. The study has never been replicated in humans, so it is not clear yet if the findings apply to humans as in the case of mice. However, the results of the study are the jumping-off points for future research on human breast milk stem cells and their possible application in stem cell therapies. Additional studies are necessary to understand the passage of human breast milk stem cells through the neonate’s GI tract, and passage to the systemic circulation.

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Potential of Ayurvedic Drugs in Differentiating Neuronal Stem Cells from Human Breast Milk: A Review

Nepal Journal of Biotechnology ◽

10.3126/njb.v7i1.26956 ◽

2019 ◽

Vol 7 (1) ◽

pp. 113-123

Author(s):

Rinki Kumari

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Breast Milk ◽

Cell Types ◽

Human Mesenchymal Stem Cell ◽

Human Breast Milk ◽

Cell Renewal ◽

Human Breast ◽

Neuronal Stem Cells ◽

Ayurvedic Drugs

Recently, Stem cell therapy has revolutionized excellent clinical therapy with the potential of stem cells to differentiate into various cell types and it may help to replace different cell lines of an organism. Frequent, clinical trials are carried out to merge the new scientific stem cell information and techniques with traditional knowledge and plant extracts that may result in less toxic, affordable, and highly available natural alternative therapeutics. Ayurveda, the traditional Indian system of medicine has given great emphasis to the promotion of health. Ayurveda therapies are based on the restoration of body balance and nourishment of dhatus. Rasayana concept of Ayurveda explains tissue regeneration and cell renewal. Rasayana drugs and therapies provide research opportunities for the biology of regeneration. Specific Medhya Rasayana stimulates and nourishes respective Medha (Dhi, Driti, and Smriti) dhatus. Interpretation of this description offers clues for specific differentiation of neuronal stem cells from human breast milk (HBM) in the presence of some herbal extracts. The previous studies suggest that neuronal stem cells differentiate from Human Breast Milk (Human mesenchymal stem cell) more effectively with Madhya Rasayana drugs. The present review highlights the potential of Ayurveda and its possible contributions in regenerative medicine.

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The Therapeutic Potential of Conditioned Medium from Human Breast Milk Stem Cells in Treating Spinal Cord Injury

Asian Spine Journal ◽

10.31616/asj.2019.0026 ◽

2020 ◽

Vol 14 (2) ◽

pp. 131-138 ◽

Cited By ~ 1

Author(s):

Maryam Borhani-Haghighi ◽

Shadan Navid ◽

Yousef Mohamadi

Keyword(s):

Spinal Cord ◽

Stem Cells ◽

Spinal Cord Injury ◽

Breast Milk ◽

Conditioned Medium ◽

Rat Model ◽

Intrathecal Administration ◽

Human Breast Milk ◽

Human Breast ◽

Cord Injury

Study Design: Experimental animal study.Purpose: This study investigated the therapeutic effects of human breast milk stem cell (BMSC)-conditioned medium (BMSC-CM) in a model of spinal cord injury (SCI) in male Sprague-Dawley rats.Overview of Literature: SCI is one of the leading causes of disability in addition to sensory and motor impairment. So far, there have been no successful treatments for SCI. Given the positive outcomes associated with using stem cells and their derivatives as a treatment for various diseases, there is a growing interest in using them as an SCI treatment. Recent research has demonstrated that CM from stem cells has therapeutic advantages.Methods: Human BMSCs were isolated and characterized, and CM was subsequently collected. Animals received an intrathecal administration of BMSC-CM after SCI. The activity of caspase-3 was measured to assess apoptosis, and levels of tumor necrosis factor-α and interleukin-1β were measured to assess inflammation. Also, sensory and locomotor performances were assessed after SCI and BMSC-CM administration.Results: Administration of CM from BMSC reduced apoptosis and inflammation at the site of injury in a rat model of SCI (p<0.05). Motor, sensory, locomotor, and sensorimotor performances were significantly improved in rats that received BMSC-CM after SCI.Conclusions: Intrathecal administration of BMSC-CM improved recovery in a rat model of SCI.

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