scholarly journals Quorum Sensing Activity ofMesorhizobiumsp. F7 Isolated from Potable Water

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Pei-Ling Yong ◽  
Kok-Gan Chan
Keyword(s):  
Mass Spectrometry ◽  
Phylogenetic Analysis ◽  
Potable Water ◽  
Bacterial Isolate ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Triple Quadrupole ◽  
Liquid Chromatography Mass Spectrometry ◽  
16S Rdna Gene ◽  
Microbe Interactions

We isolated a bacterial isolate (F7) from potable water. The strain was identified asMesorhizobiumsp. by 16S rDNA gene phylogenetic analysis and screened forN-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) which confirmed the production of multiple AHLs, namely,N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8-HSL) andN-3-oxo-decanoyl-L-homoserine lactone (3-oxo-C10-HSL). These findings will open the perspective to study the function of these AHLs in plant-microbe interactions.

scholarly journals Quorum-Sensing Signal Synthesis by the Yersinia pestis Acyl-Homoserine Lactone Synthase YspI

2006 ◽  
Vol 188 (2) ◽  
pp. 784-788 ◽  
Author(s):  
J. Paul Kirwan ◽  
Ty A. Gould ◽  
Herbert P. Schweizer ◽  
Scott W. Bearden ◽  
Robert C. Murphy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Yersinia Pestis ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Liquid Chromatography Mass Spectrometry ◽  
Wild Type ◽  
Physical Chemical ◽  
Signal Synthesis ◽  
Chemical Techniques ◽  
Culture Supernatants

ABSTRACT The acyl-homoserine lactone molecular species (AHLs) produced by the Yersinia pestis AHL synthase YspI were identified by biochemical and physical/chemical techniques. Bioassays of extracts from culture supernatants of the recombinant YspI and wild-type Yersinia pestis showed similar profiles of AHLs. Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-l-homoserine lactone and N-3-oxo-hexanoyl-l-homoserine lactone.

scholarly journals Analysis of allergenic residues in wines by triple quadrupole LCMS

2019 ◽  
Vol 12 ◽  
pp. 04012
Author(s):  
F.R. Spinelli ◽  
G.J. Cargnel ◽  
A.P. Drehmer ◽  
C. Blatt ◽  
M. Baptistão ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Triple Quadrupole ◽  
Liquid Chromatography Mass Spectrometry ◽  
Treatment Practices ◽  
Unambiguous Determination ◽  
Allergenic Proteins ◽  
Beta Casein ◽  
Fining Agents ◽  
Low Levels

During the winemaking are used technology coadjuvants, between them: albumin, caseinates and lysozyme. These compounds have great oenological properties, however, the presence of their residues can represent risks to people who are allergic to them because they are derived from eggs and milk. Mass spectrometry methods enables unambiguous determination of allergenic proteins at low levels in wines. Therefore, the aim of this study was to determine the concentrations of ovalbumin, alpha-casein, beta-casein and lysozyme in experimental wines treated with different concentrations of them by triple quadrupole liquid chromatography mass spectrometry with Jet Stream Electrospray Ionization (ESI). The wines were elaborated and treated with different concentrations of albumin, lysozyme and potassium caseinate. Bentonite and decantation were used for the removal of the fining agents. The quantification limits (LOQ) for ovalbumin, a-casein, b-casein and lysozyme were: 0.002 mg/L, 0.24 mg/L, 0.75 mg/L and 0.04 mg/L, respectively. Non residues of the proteins were identified in the experimental wines treated with the different amounts of potassium caseinate, albumin and lysozyme, analyzed in this study. These results provide an evidence of the absence of residues of caseinate, albumin and lysozyme in the concentrations tested in the wines if good treatment practices are followed.

scholarly journals Pantoeasp. Isolated from Tropical Fresh Water ExhibitingN-Acyl Homoserine Lactone Production

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Wen-Si Tan ◽  
Nina Yusrina Muhamad Yunos ◽  
Pui-Wan Tan ◽  
Nur Izzati Mohamad ◽  
Tan-Guan-Sheng Adrian ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Sequence Similarity ◽  
Environmental Water ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Gram Negative Bacteria ◽  
Water Sampling ◽  
16S Rdna Gene ◽  
Preliminary Screening ◽  
Pantoea Stewartii

N-Acyl homoserine lactone (AHL) serves as signaling molecule for quorum sensing (QS) in Gram-negative bacteria to regulate various physiological activities including pathogenicity. With the aim of isolating freshwater-borne bacteria that can cause outbreak of disease in plants and portrayed QS properties, environmental water sampling was conducted. Here we report the preliminary screening of AHL production usingChromobacterium violaceumCV026 andEscherichia coli[pSB401] as AHL biosensors. The 16S rDNA gene sequence of isolate M009 showed the highest sequence similarity toPantoea stewartiiS9-116, which is a plant pathogen. The isolatedPantoeasp. was confirmed to produceN-3-oxohexanoyl-L-HSL (3-oxo-C6-HSL) through analysis of high resolution mass tandem mass spectrometry.

scholarly journals Nitrite-Oxidizing Bacterium Nitrobacter winogradskyi ProducesN-Acyl-Homoserine Lactone Autoinducers

2015 ◽  
Vol 81 (17) ◽  
pp. 5917-5926 ◽  
Author(s):  
Brett L. Mellbye ◽  
Peter J. Bottomley ◽  
Luis A. Sayavedra-Soto
Keyword(s):  
Mass Spectrometry ◽  
Chemostat Culture ◽  
Rhodopseudomonas Palustris ◽  
Homoserine Lactone ◽  
Amino Acid Sequences ◽  
Acyl Homoserine Lactone ◽  
Dependent Manner ◽  
Content Type ◽  
Genes Encoding ◽  
A Cell

ABSTRACTNitrobacter winogradskyiis a chemolithotrophic bacterium that plays a role in the nitrogen cycle by oxidizing nitrite to nitrate. Here, we demonstrate a functionalN-acyl-homoserine lactone (acyl-HSL) synthase in this bacterium. TheN. winogradskyigenome contains genes encoding a putative acyl-HSL autoinducer synthase (nwi0626,nwiI) and a putative acyl-HSL autoinducer receptor (nwi0627,nwiR) with amino acid sequences 38 to 78% identical to those inRhodopseudomonas palustrisand otherRhizobiales. Expression ofnwiIandnwiRcorrelated with acyl-HSL production during culture.N. winogradskyiproduces two distinct acyl-HSLs,N-decanoyl-l-homoserine lactone (C10-HSL) and a monounsaturated acyl-HSL (C10:1-HSL), in a cell-density- and growth phase-dependent manner, during batch and chemostat culture. The acyl-HSLs were detected by bioassay and identified by ultraperformance liquid chromatography with information-dependent acquisition mass spectrometry (UPLC-IDA-MS). The C=C bond in C10:1-HSL was confirmed by conversion into bromohydrin and detection by UPLC-IDA-MS.

scholarly journals Enterobacter cancerogenus produces short chain N-3-oxo-acylhomoserine lactones quorum sensing molecules

2015 ◽  
Author(s):  
Kok-Gan Chan ◽  
Wen-Si Tan
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Mass Spectrometry Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Synthesis Activity

Enterobacter cancerogenus strain M004 genome size is 5.67 Mb. Here, its luxI homologue, designated as ecnI which is ecnI gene (633 bp) was cloned and overexpressed. Its AHL synthesis activity was verified using the high-resolution liquid chromatography-mass spectrometry analysis revealed the production of N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) and N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C8-HSL). The cloning and characterization of luxI homologue of E. cancerogenus strain M004 was firstly reported here.

scholarly journals Discovery of Pandoraea pnomenusa RB38 N-acyl homoserine Lactone Synthase (PpnI) and its Complete Genome Sequence Analysis

2015 ◽  
Author(s):  
Kok-Gan Chan ◽  
Robson Ee ◽  
Kah-Yan How ◽  
Siew-Kim Lee ◽  
Wai-Fong Yin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Homoserine Lactone ◽  
Tandem Mass ◽  
Acyl Homoserine Lactone ◽  
Multiple Sequence ◽  
Genome Sequence Analysis ◽  
Luxr Homolog ◽  
Distinct Cluster ◽  
Complete Genome Sequence Analysis ◽  
Phylogenetic Neighbor

In this study, we sequenced the genome of P. pnomenusa RB38 and reported the finding of a pair of cognate luxI/R homologs which we firstly coined as ppnI, which is found adjacent to a luxR homolog, ppnR. An additional orphan luxR homolog, ppnR2 was also discovered. Multiple sequence alignment revealed that PpnI is a distinct cluster of AHL synthase compared to those of its nearest phylogenetic neighbor, Burkholderia spp. When expressed heterologously and analysed using high resolution tandem mass spectrometry, PpnI directs the synthesis of N-octanoylhomoserine lactone (C8-HSL). To our knowledge, this is the first documentation of the luxI/R homologs of the genus of Pandoraea.

scholarly journals Enterobacter cancerogenus produces short chain N-3-oxo-acylhomoserine lactones quorum sensing molecules

2015 ◽  
Author(s):  
Kok-Gan Chan ◽  
Wen-Si Tan
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Mass Spectrometry Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Synthesis Activity

Enterobacter cancerogenus strain M004 genome size is 5.67 Mb. Here, its luxI homologue, designated as ecnI which is ecnI gene (633 bp) was cloned and overexpressed. Its AHL synthesis activity was verified using the high-resolution liquid chromatography-mass spectrometry analysis revealed the production of N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) and N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C8-HSL). The cloning and characterization of luxI homologue of E. cancerogenus strain M004 was firstly reported here.

scholarly journals Determination of Chlormequat in Pears by Liquid Chromatography/Mass Spectrometry

2000 ◽  
Vol 83 (3) ◽  
pp. 742-747 ◽  
Author(s):  
Hans G J Mol ◽  
Ruud C J Van Dam ◽  
Rob J Vreeken ◽  
Odile M Steijger
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Trap ◽  
Triple Quadrupole ◽  
Liquid Chromatography Mass Spectrometry ◽  
Liquid Chromatograph ◽  
Triple Quadrupole Instrument ◽  
Chromatography Mass Spectrometry ◽  
Quadrupole Instrument

Abstract A straightforward and reliable method was developed for the determination of chlormequat in pears by liquid chromatography/mass spectrometry (LC/MS). Water and methanol were compared as extraction solvents. Because no significant differences in extraction efficiency or repeatability were found, water was chosen as the extraction solvent. The extracts were analyzed without cleanup by either an ion-trap liquid chromatograph/mass spectrometer in the single MS mode or a triple-quadrupole instrument in the MS/MS mode, using electrospray ionization. Both instruments were equally suitable for quantitation and confirmation of identity. Recoveries were 76–103%, and reproducibility was ≤12%. The lowest detection limit (0.007 mg/kg) was obtained with the triple-quadrupole instrument in the MS/MS mode.

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