scholarly journals Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Agnieszka Zebrowska ◽  
Malgorzata Wagrowska-Danilewicz ◽  
Marian Danilewicz ◽  
Olga Stasikowska-Kanicka ◽  
Lilianna Kulczycka-Siennicka ◽  
...  
Keyword(s):  
Mast Cells ◽  
Bullous Pemphigoid ◽  
Skin Diseases ◽  
Dermatitis Herpetiformis ◽  
Skin Lesions ◽  
Autoimmune Blistering Disease ◽  
Mmp9 Expression ◽  
Skin Biopsies ◽  
Cell Expression ◽  
Perilesional Skin

Bullous pemphigoid (BP) and dermatitis herpetiformis (DH) are skin diseases associated with inflammation. However, few findings exist concerning the role of mast cells in autoimmune blistering disease. Skin biopsies were taken from 27 BP and 14 DH patients, as well as 20 healthy individuals. Immunohistochemistry was used to identify the localization and mast cell expression of TNFα and MMP9 in skin lesions and perilesional skin. The serum concentrations of TNFα, MMP9, chymase, tryptase, PAF, and IL-4 were measured by immunoassay. TNFα and MMP9 expression in the epidermis and in inflammatory influxed cells in the dermis was detected in skin biopsies from patients. Although these mediators were found to be expressed in the perilesional skin of all patients, the level was much lower than that in lesional skin. Increased serum PAF levels were observed in BP patients. Mast cells may play an essential role in activating inflammation, which ultimately contributes to the tissue damage observed in BP and DH. Our findings suggest that differences in the pattern of cytokine expression directly contribute to variations in cellular infiltration in DH and BP.

scholarly journals IL-17 Expression in Dermatitis Herpetiformis and Bullous Pemphigoid

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Agnieszka Zebrowska ◽  
Malgorzata Wagrowska-Danilewicz ◽  
Marian Danilewicz ◽  
Olga Stasikowska-Kanicka ◽  
Anna Cynkier ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Healthy Subjects ◽  
Inflammatory Process ◽  
Essential Role ◽  
Skin Diseases ◽  
Dermatitis Herpetiformis ◽  
Control Group ◽  
Bullous Diseases ◽  
Skin Biopsies ◽  
Perilesional Skin

Dermatitis herpetiformis (DH) and bullous pemphigoid (BP) are skin diseases associated with eosinophilic and neutrophilic infiltrations. Although cytokines are critical for the inflammatory process, there are single findings concerning concentration of IL-17 in bullous diseases. The goal of this study was to assess IL-17 expression in DH and BP patients. Skin biopsies were taken from 10 DH, 14 BP patients and from 10 healthy subjects. The localization and expression of IL-17 was studied by immunohistochemistry and the serum concentration was measured by immunoassays. Expression of IL-17 in the epidermis and in influxed cells in dermis was detected in skin biopsies. Expression of IL-17 was statistically higher in epidermis and infiltration cells in specimens from BP than from DH patients. Examined interleukin expression was detected in perilesional skin of all patients but it was much lower than in lesional skin. The expression of IL-17 was not observed in biopsies from healthy people. Serum level of IL-17 was statistically higher in BP and DH groups as compared to control group. Our results provide the evidence that IL-17 may play an essential role in activating and recruiting eosinophils and neutrophils, which ultimately contribute to the tissue damage in DH and BP.

scholarly journals Tissue Factor in Dermatitis Herpetiformis and Bullous Pemphigoid: Link between Immune and Coagulation System in Subepidermal Autoimmune Bullous Diseases

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Agnieszka Zebrowska ◽  
Malgorzata Wagrowska-Danilewicz ◽  
Marian Danilewicz ◽  
Joanna Wieczfinska ◽  
Ewa Pniewska ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Bullous Pemphigoid ◽  
Skin Diseases ◽  
Dermatitis Herpetiformis ◽  
Plasma Concentrations ◽  
Coagulation System ◽  
Variable Expression ◽  
Autoimmune Bullous Diseases ◽  
Skin Biopsies ◽  
Perilesional Skin

Dermatitis herpetiformis (DH) and bullous pemphigoid (BP) are skin diseases associated with eosinophilic and neutrophilic infiltrations. Although chemokines are critical for the selective accumulation and activation of various leukocyte subsets in the inflammatory process, there are few findings concerning inflammatory cells and production of coagulation factors in blistering diseases. Skin biopsies were taken from 14 patients with DH, 27 with BP, and 20 control subjects. The localization and expression of tissue factor (TF) in skin lesions and perilesional skin were studied by immunohistochemistry and confirmed by Western Blot. Moreover the plasma concentrations of TF were measured by immunoassays. D dimers, fibrinogen, and selected coagulation parameters were measured by routine methods. Expression of TF in the epidermis and in inflammatory influxed cells in dermis was detected in skin biopsies from BP patients. Examined TF expression was detected in perilesional skin of all BP patients too. The expression of TF was not observed in biopsies from healthy people and DH patients. The findings of the study show an increased expression of tissue factor in the lesional and perilesional skin of patients with bullous pemphigoid. The difference in chemokine pattern expression and variations in the cellular infiltration in BP and DH cause variable expression of TF.

The Role of Apoptosis in the Pathogenesis of Dermatitis Herpetiformis

2005 ◽  
Vol 18 (4) ◽  
pp. 691-699 ◽  
Author(s):  
M. Caproni ◽  
D. Torchia ◽  
E. Antiga ◽  
D. Degl'Innocenti ◽  
E. Barletta ◽  
...  
Keyword(s):  
Fas Ligand ◽  
Skin Diseases ◽  
Dermatitis Herpetiformis ◽  
Basal Layer ◽  
Immunohistochemical Analysis ◽  
Skin Lesions ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Cutaneous Lesions ◽  
Malignant Skin ◽  
Perilesional Skin

Apoptosis is a form of cell death that is claimed to be involved in a number of chronic inflammatory and malignant skin diseases. The aim of this study was to investigate whether apoptosis may contribute to the pathogenesis of epidermal changes in dermatitis herpetiformis (DH) and, in particular, whether certain apoptosis-related markers such as Bax, Bcl-2, Fas and Fas ligand (FasL) take part in this process. For the detection of apoptotic nuclei, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelling technique (TUNEL) was employed on cryostat sections. Skin lesions from six and perilesional skin from four DH patients were stained with monoclonal antibodies to Bax, Bcl-2, Fas and FasL. The same evaluation was also performed on three patients affected by bullous pemphigoid (BP) and in two healthy donors. Using TUNEL technique, a remarkable increase in the apoptotic rate within the epidermal compartment was observed in DH and BP patients in comparison with normal controls. In our immunohistochemical analysis, Bax/Bcl-2 ratio was almost the same in the epidermis of perilesional/lesional DH, BP and healthy skin specimens. In DH and BP specimens both Bax and Bcl-2 proteins were increased in the dermal perivascular compartment. Fas showed a prevalently epidermal staining, both in DH and BP lesions, while FasL was distributed in perivascular and subjunctional dermis; some FasL+ cells infiltrated the DEJ and the basal layer of epidermis. This study allowed us to highlight conspicuous apoptotic phenomena in basal and suprabasal keratinocytes within lesional and perilesional skin of DH. We conclude that in DH, as well as in BP, apoptosis plays a role in the pathogenesis of cutaneous lesions in concert with other pathogenetic mechanisms.

scholarly journals The Expression of Selected Proapoptotic Molecules in Dermatitis Herpetiformis

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Zebrowska Agnieszka ◽  
Erkiert-Polguj Anna ◽  
Wagrowska-Danilewicz Malgorzata ◽  
Danilewicz Marian ◽  
Sysa-Jedrzejowska Anna ◽  
...  
Keyword(s):  
Fas Ligand ◽  
Dermatitis Herpetiformis ◽  
Single Cells ◽  
Skin Lesions ◽  
Control Group ◽  
Trail Expression ◽  
Skin Biopsies ◽  
And Control ◽  
Perilesional Skin

The role of the process of apoptosis is investigated in the pathogenesis of many autoimmune diseases; however at present, there is not much information about its role in dermatitis herpetiformis. Skin biopsies were taken from 18 DH patients and from 10 healthy subjects. The localization and expression of Bax, Fas, FasL, TRAIL, TRAIL-R in skin lesions, and perilesional skin were studied by immunohistochemistry. Expression of Bax, Fas, and Fas ligand was detected in the keratinocytes in skin biopsies from DH patients. Expression of TRAIL and TRAIL receptor was confirmed in epidermis, infiltration cells, and some fibroblasts. The expression of examined molecules in biopsies from healthy people was observed only in single cells. There were statistically significant differences between lesional, perilesional, and healthy skin of control group in Bax expression analysis and between lesional skin and control group in Fas, FasL, and TRAIL expression. There were statistically significant differences between control group and perilesional skin in Bax and FasL expression. Our results show that selected proapoptotic molecules may take part in pathogenesis of dermatitis herpetiformis, but the role of apoptosis in this process is not clear.

scholarly journals Profile of Skin Biopsies and Patterns of Granulomatous Skin Diseases in a Tertiary Care Center of Western Nepal

2020 ◽  
Vol 3 (2) ◽  
pp. 288-293
Author(s):  
Ajay Kumar ◽  
Saujan Subedi ◽  
Jenny Pun ◽  
Binamra Basnet ◽  
Raja Babu Giri ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Care Center ◽  
Tertiary Care ◽  
Cost Effective ◽  
Skin Lesions ◽  
Clinical Impression ◽  
Clinicopathological Correlation ◽  
Diagnostic Challenge ◽  
Cross Sectional ◽  
Skin Biopsies

Background: Granulomatous skin diseases are one of the leading causes of morbidity in tropical countries like Nepal. These granulomatous skin lesions often pose diagnostic challenge to clinicians as well as to dermatopathologists. Histopathologic examination of a biopsy specimen represents one of the most informative and cost-effective procedure and may help to avoid other, costlier and invasive diagnostic workup. Materials and Methods: This cross-sectional observational study from October 2018 to October 2019, at department of dermatology, enrolled 142 cases of skin biopsies. Correlation between clinical impression and histopathological findings was evaluated. Results: Out of 13940 dermatology visits/ consultations, 142 (1.01%) skin biopsies were performed. Head, neck and face were the commonest sites of biopsies (29.6%). The most common biopsy technique was incisional type in 70 (50.4%). Histopathology showed granulomatous features in 49 (34.8%) cases, out of which tuberculoid type was the commonest, in 29 (58.0%). Positive clinicopathological correlation was seen in 117/142 (82.4%) for all biopsies and 41/49 (85%) for granulomaous skin lesions. Conclusion: Tuberculoid type was the most common cutaneous granuloma. High clinicopathological correlation in our study supports histopathology as an important tool for diagnosis of granulomatous as well overall skin disorders.

scholarly journals The Role of Intereukin-31 in Pathogenesis of Itch and Its Intensity in a Course of Bullous Pemphigoid and Dermatitis Herpetiformis

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Lilianna Kulczycka-Siennicka ◽  
Anna Cynkier ◽  
Elżbieta Waszczykowska ◽  
Anna Woźniacka ◽  
Agnieszka Żebrowska
Keyword(s):  
Signaling Pathway ◽  
Bullous Pemphigoid ◽  
Dermatitis Herpetiformis ◽  
Subjective Symptoms ◽  
Autoimmune Blistering Disease ◽  
Stat Signaling ◽  
Blistering Disease ◽  
Autoimmune Blistering Diseases

Itch which is one of the major, subjective symptoms in a course of bullous pemphigoid and dermatitis herpetiformis makes those two diseases totally different than other autoimmune blistering diseases. Its pathogenesis is still not fully known. The aim of this research was to assess the role of IL-31 in development of itch as well as to measure its intensity. Obtained results, as well as literature data, show that lower concentration of IL-31 in patients’ serum may be correlated with its role in JAK/STAT signaling pathway which is involved in development of autoimmune blistering disease. Intensity of itch is surprisingly huge problem for the patients and the obtained results are comparable with results presented by atopic patients.

scholarly journals Expression of the JAK/STAT Signaling Pathway in Bullous Pemphigoid and Dermatitis Herpetiformis

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
K. Juczynska ◽  
A. Wozniacka ◽  
E. Waszczykowska ◽  
M. Danilewicz ◽  
M. Wagrowska-Danilewicz ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Bullous Pemphigoid ◽  
Target Genes ◽  
Dermatitis Herpetiformis ◽  
Skin Lesions ◽  
Control Group ◽  
Cytokine Network ◽  
Activation Of Transcription ◽  
Autoimmune Bullous Diseases ◽  
Stat Signaling

A family of eleven proteins comprises the Janus kinases (JAK) and signal transducers and activators of transcription (STAT) signaling pathway, which enables transduction of signal from cytokine receptor to the nucleus and activation of transcription of target genes. Irregular functioning of the cascade may contribute to pathogenesis of autoimmune diseases; however, there are no reports concerning autoimmune bullous diseases yet to be published. The aim of this study was to evaluate the expression of proteins constituting the JAK/STAT signaling pathway in skin lesions and perilesional area in dermatitis herpetiformis (DH) and bullous pemphigoid (BP), as well as in the control group. Skin biopsies were collected from 21 DH patients, from 20 BP patients, and from 10 healthy volunteers. The localization and expression of selected STAT and JAK proteins were examined by immunohistochemistry and immunoblotting. We found significantly higher expression of JAK/STAT proteins in skin lesions in patients with BP and DH, in comparison to perilesional skin and the control group, which may be related to proinflammatory cytokine network and induction of inflammatory infiltrate in tissues. Our findings suggest that differences in the JAK and STAT expression may be related to distinct cytokines activating them and mediating neutrophilic and/or eosinophilic infiltrate.

scholarly journals The Imbalance Between Metalloproteinases and Their Tissue Inhibitors Is Involved in the Pathogenesis of Dermatitis Herpetiformis

2005 ◽  
Vol 2005 (6) ◽  
pp. 373-379 ◽  
Author(s):  
Agnieszka Zebrowska ◽  
Joanna Narbutt ◽  
Anna Sysa-Jedrzejowska ◽  
Jozef Kobos ◽  
Elzbieta Waszczykowska
Keyword(s):  
Autoimmune Disease ◽  
Coeliac Disease ◽  
Healthy Subjects ◽  
Dermatitis Herpetiformis ◽  
Skin Lesions ◽  
Blister Fluid ◽  
Exact Role ◽  
Basal Keratinocytes ◽  
Intestinal Lesions ◽  
Skin Biopsies

Dermatitis herpetiformis (DH) is a subepidermal autoimmune disease characterized by skin and intestinal lesions consistent with coeliac disease. There are also some data that metalloproteinases (MMPs) are involved in the development of skin lesions in DH, however their exact role in this process is not fully understood. The aim of the study was to investigate whether MMPs and their inhibitors are involved in pathogenesis of DH. Skin biopsies were taken from 13 patients with active DH and from 10 healthy subjects. The localization and expression of MMPs and TIMPs were examined by immunohistochemistry. MMPs expression was detected in basal keratinocytes and in the whole epidermis in all of the DH subjects. Neutrophils in microabscesses and in blister fluid were also positive for MMPs. Expression of TIMPs was moderate or weak in all examined biopsies. Our results allow us to conclude that imbalance between these enzymes takes an important role in the pathogenesis of DH.

scholarly journals Granulomatous lesions of the skin

1970 ◽  
Vol 1 (2) ◽  
pp. 81-86 ◽  
Author(s):  
K Gautam ◽  
RR Pai ◽  
S Bhat
Keyword(s):  
Skin Diseases ◽  
Histopathological Examination ◽  
Skin Lesions ◽  
Diagnostic Challenge ◽  
Modes Of Presentation ◽  
Supportive Role ◽  
Granulomatous Lesions ◽  
Granulomatous Dermatitis ◽  
Skin Biopsies

Background: Granulomatous skin lesions often present as a diagnostic challenge to dermatopathologists due to various modes of presentation and identical histological picture produced by several causes. The aim of the study was to study different granulomatous skin lesions and to determine the relative frequency, the level of clinicopathologic concordance and to compare our results with those of other workers. Materials and Methods: A retrospective analysis of skin biopsies received over a period of two years from January 2007 to December 2008; was performed, and cases of granulomatous dermatitis reported on histopathological examination were reviewed along with special stains. Results: Out of a total of 1590 skin biopsies 106 (6.67%) cases were found to have a granulomatous reaction. It was common in males (63.21%) with most occurring in the fourth to fifth decades. Majority of cases (79 cases, 74.5%) were categorized as infectious granulomatous lesions with predominance of leprosy (63 cases, 79.7%) followed by tuberculosis (6 cases, 7.6%). An overall clinicopathologic concordance was seen in 97% of cases of leprosy. Conclusion: In this study leprosy is the most common cause of granulomatous skin lesions. It can be concluded that histopathology plays an important role in classification of leprosy, and in diagnosis and management of a variety of granulomatous skin diseases. Special stains play a supportive role in infectious granulomas. Keywords: Granulomatous skin lesion; Leprosy; Skin biopsy DOI: http://dx.doi.org/10.3126/jpn.v1i2.5397 JPN 2011; 1(2): 81-86

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