The Expression of Selected Proapoptotic Molecules in Dermatitis Herpetiformis

The role of the process of apoptosis is investigated in the pathogenesis of many autoimmune diseases; however at present, there is not much information about its role in dermatitis herpetiformis. Skin biopsies were taken from 18 DH patients and from 10 healthy subjects. The localization and expression of Bax, Fas, FasL, TRAIL, TRAIL-R in skin lesions, and perilesional skin were studied by immunohistochemistry. Expression of Bax, Fas, and Fas ligand was detected in the keratinocytes in skin biopsies from DH patients. Expression of TRAIL and TRAIL receptor was confirmed in epidermis, infiltration cells, and some fibroblasts. The expression of examined molecules in biopsies from healthy people was observed only in single cells. There were statistically significant differences between lesional, perilesional, and healthy skin of control group in Bax expression analysis and between lesional skin and control group in Fas, FasL, and TRAIL expression. There were statistically significant differences between control group and perilesional skin in Bax and FasL expression. Our results show that selected proapoptotic molecules may take part in pathogenesis of dermatitis herpetiformis, but the role of apoptosis in this process is not clear.

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The Role of Apoptosis in the Pathogenesis of Dermatitis Herpetiformis

International Journal of Immunopathology and Pharmacology ◽

10.1177/039463200501800411 ◽

2005 ◽

Vol 18 (4) ◽

pp. 691-699 ◽

Cited By ~ 11

Author(s):

M. Caproni ◽

D. Torchia ◽

E. Antiga ◽

D. Degl'Innocenti ◽

E. Barletta ◽

...

Keyword(s):

Fas Ligand ◽

Skin Diseases ◽

Dermatitis Herpetiformis ◽

Basal Layer ◽

Immunohistochemical Analysis ◽

Skin Lesions ◽

Terminal Deoxynucleotidyl Transferase ◽

Cutaneous Lesions ◽

Malignant Skin ◽

Perilesional Skin

Apoptosis is a form of cell death that is claimed to be involved in a number of chronic inflammatory and malignant skin diseases. The aim of this study was to investigate whether apoptosis may contribute to the pathogenesis of epidermal changes in dermatitis herpetiformis (DH) and, in particular, whether certain apoptosis-related markers such as Bax, Bcl-2, Fas and Fas ligand (FasL) take part in this process. For the detection of apoptotic nuclei, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelling technique (TUNEL) was employed on cryostat sections. Skin lesions from six and perilesional skin from four DH patients were stained with monoclonal antibodies to Bax, Bcl-2, Fas and FasL. The same evaluation was also performed on three patients affected by bullous pemphigoid (BP) and in two healthy donors. Using TUNEL technique, a remarkable increase in the apoptotic rate within the epidermal compartment was observed in DH and BP patients in comparison with normal controls. In our immunohistochemical analysis, Bax/Bcl-2 ratio was almost the same in the epidermis of perilesional/lesional DH, BP and healthy skin specimens. In DH and BP specimens both Bax and Bcl-2 proteins were increased in the dermal perivascular compartment. Fas showed a prevalently epidermal staining, both in DH and BP lesions, while FasL was distributed in perivascular and subjunctional dermis; some FasL+ cells infiltrated the DEJ and the basal layer of epidermis. This study allowed us to highlight conspicuous apoptotic phenomena in basal and suprabasal keratinocytes within lesional and perilesional skin of DH. We conclude that in DH, as well as in BP, apoptosis plays a role in the pathogenesis of cutaneous lesions in concert with other pathogenetic mechanisms.

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Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis

Mediators of Inflammation ◽

10.1155/2014/936545 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 10

Author(s):

Agnieszka Zebrowska ◽

Malgorzata Wagrowska-Danilewicz ◽

Marian Danilewicz ◽

Olga Stasikowska-Kanicka ◽

Lilianna Kulczycka-Siennicka ◽

...

Keyword(s):

Mast Cells ◽

Bullous Pemphigoid ◽

Skin Diseases ◽

Dermatitis Herpetiformis ◽

Skin Lesions ◽

Autoimmune Blistering Disease ◽

Mmp9 Expression ◽

Skin Biopsies ◽

Cell Expression ◽

Perilesional Skin

Bullous pemphigoid (BP) and dermatitis herpetiformis (DH) are skin diseases associated with inflammation. However, few findings exist concerning the role of mast cells in autoimmune blistering disease. Skin biopsies were taken from 27 BP and 14 DH patients, as well as 20 healthy individuals. Immunohistochemistry was used to identify the localization and mast cell expression of TNFα and MMP9 in skin lesions and perilesional skin. The serum concentrations of TNFα, MMP9, chymase, tryptase, PAF, and IL-4 were measured by immunoassay. TNFα and MMP9 expression in the epidermis and in inflammatory influxed cells in the dermis was detected in skin biopsies from patients. Although these mediators were found to be expressed in the perilesional skin of all patients, the level was much lower than that in lesional skin. Increased serum PAF levels were observed in BP patients. Mast cells may play an essential role in activating inflammation, which ultimately contributes to the tissue damage observed in BP and DH. Our findings suggest that differences in the pattern of cytokine expression directly contribute to variations in cellular infiltration in DH and BP.

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IL-17 Expression in Dermatitis Herpetiformis and Bullous Pemphigoid

Mediators of Inflammation ◽

10.1155/2013/967987 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 23

Author(s):

Agnieszka Zebrowska ◽

Malgorzata Wagrowska-Danilewicz ◽

Marian Danilewicz ◽

Olga Stasikowska-Kanicka ◽

Anna Cynkier ◽

...

Keyword(s):

Bullous Pemphigoid ◽

Healthy Subjects ◽

Inflammatory Process ◽

Essential Role ◽

Skin Diseases ◽

Dermatitis Herpetiformis ◽

Control Group ◽

Bullous Diseases ◽

Skin Biopsies ◽

Perilesional Skin

Dermatitis herpetiformis (DH) and bullous pemphigoid (BP) are skin diseases associated with eosinophilic and neutrophilic infiltrations. Although cytokines are critical for the inflammatory process, there are single findings concerning concentration of IL-17 in bullous diseases. The goal of this study was to assess IL-17 expression in DH and BP patients. Skin biopsies were taken from 10 DH, 14 BP patients and from 10 healthy subjects. The localization and expression of IL-17 was studied by immunohistochemistry and the serum concentration was measured by immunoassays. Expression of IL-17 in the epidermis and in influxed cells in dermis was detected in skin biopsies. Expression of IL-17 was statistically higher in epidermis and infiltration cells in specimens from BP than from DH patients. Examined interleukin expression was detected in perilesional skin of all patients but it was much lower than in lesional skin. The expression of IL-17 was not observed in biopsies from healthy people. Serum level of IL-17 was statistically higher in BP and DH groups as compared to control group. Our results provide the evidence that IL-17 may play an essential role in activating and recruiting eosinophils and neutrophils, which ultimately contribute to the tissue damage in DH and BP.

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The Role of Fas-FasL Signaling Pathway in Induction of Apoptosis in Patients with Sulfur Mustard-Induced Chronic Bronchiolitis

Journal of Toxicology ◽

10.1155/2010/373612 ◽

2010 ◽

Vol 2010 ◽

pp. 1-7 ◽

Cited By ~ 17

Author(s):

Gila Pirzad ◽

Mahvash Jafari ◽

Sasan Tavana ◽

Homayoon Sadrayee ◽

Saeid Ghavami ◽

...

Keyword(s):

Lung Injury ◽

Signaling Pathway ◽

Caspase 3 ◽

Fas Ligand ◽

Sulfur Mustard ◽

Control Group ◽

Induced Apoptosis ◽

Apoptosis And Necrosis ◽

And Control

Sulfur mustard (SM) is an alkylating agent that induces apoptosis and necrosis in cells. Fas-Fas ligand (FasL) interaction could induce apoptosis as well. In this study, it was hypothesized that apoptosis might play an important role in the pathogenesis of SM-induced lung injury via Fas-FasL signaling pathway. In a case-control study, Fas and FasL levels, caspase-3 activity and percent of apoptotic cells were measured in bronchoalveolar lavage (BAL) fluid of patients 20 years after exposure to sulfur mustard and compared with the control group. Results show that Fas and FasL levels were significantly higher in BAL fluid cells in patients group compared with the control (P=.001). No significant differences were observed between mild and moderate-severe groups. BAL fluid cells caspase-3 activity was not significantly different among the mild, moderate-severe, and control groups. The data suggest that Fas-FasL-induced apoptosis was impaired in BAL fluid cells of SM-exposed patients which might be one of the initiators of pathogenesis in SM-induced lung injury in these patients.

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The Estimation of prostate specific antigen (PSA) concentrations in patients with prostatitis by fully automated ELISA technique.

Journal of Medical Research and Health Sciences ◽

10.15520/jmrhs.v3i11.279 ◽

2020 ◽

Vol 3 (11) ◽

pp. 1100-1104

Author(s):

Hussein Naeem Aldhaheri ◽

Ihsan Edan AlSaimary ◽

Murtadha Mohammed ALMusafer

Keyword(s):

Personal Information ◽

Receptor Gene ◽

Specific Antigen ◽

Gene Clusters ◽

Control Group ◽

P Value ◽

Group Data ◽

Elisa Technique ◽

And Control

The Aim of this study was to determine Immunogenetic expression of Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men from Basrah and Maysan provinces. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected. This study shows the effect of PSA level in patients with prostatitis and control group, with P-value <0.0001 therefore the study shows a positive significant between elevated PSA levels and Prostatitis.

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Role of Nutrition Counseling and Lifestyle Modification in Managing Prediabetes

Food and Nutrition Bulletin ◽

10.1177/03795721211025434 ◽

2021 ◽

pp. 037957212110254

Author(s):

Harleen Kaur ◽

Neerja Singla ◽

Rohini Jain

Keyword(s):

Hemoglobin A1c ◽

Lifestyle Modification ◽

Fasting Blood Glucose ◽

Nutrition Intervention ◽

Control Group ◽

Population Prevalence ◽

Significant Difference ◽

And Control ◽

Experimental Group

Objective: India is the second country after China having the highest population prevalence of diabetes. Several research studies investigating diabetes have been done, but not much work has been done on prediabetes. The purpose of this study was to investigate the effect of nutrition and lifestyle modification on prediabetic females. Methods: A total of 120 prediabetic females from Ludhiana city were divided into 2 matched groups: control group (n = 60) and experimental group (n = 60). Impact of nutrition intervention for dietary and lifestyle modification (for 3 months) was assessed on the anthropometric, dietary, biochemical parameters, and diabetes risk score of the experimental group and control group (no intervention). Results: All the selected 120 subjects completed the study (experimental group = 60; control group = 60). There was significant difference in the changes between the 2 groups throughout the study. The fasting blood glucose and glycated hemoglobin A1c levels of the experimental group subjects reduced significantly ( P ≤ .01). However, no change was observed among the control group subjects. The lipid profile of the experimental group showed a significant improvement ( P ≤ .01). Conclusion: Nutrition counselling of the prediabetics regarding dietary and lifestyle modification is recommended so as to improve their metabolic control, thus preventing them from being diabetics.

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A Comparison of Efficiency in Teacher Correction Strategies in Iranian EFL Learners’ Speaking Improvement

International Journal of English Linguistics ◽

10.5539/ijel.v6n6p183 ◽

2016 ◽

Vol 6 (6) ◽

pp. 183

Author(s):

Roya Keshavarz ◽

Amin Marzban

Keyword(s):

Corrective Feedback ◽

Placement Test ◽

Control Group ◽

Teaching Methodology ◽

Moderator Variable ◽

Efl Learners ◽

Feedback Type ◽

Significant Difference ◽

And Control

<p>The present study was an attempt to investigate the effect two types of corrective feedback (i.e., recast and metalinguistic) in order to find out which one is more effective on EFL learners’ speaking improvement and also to see if gender could play a role in the relative impact of the two types of corrective feedback on learners’ speaking ability. To this end, 65 EFL learners of intermediate level in one of language institutes in Shiraz, Iran were selected and divided into three groups including two experimental groups and one control. The instruments used to collect the data included IELTS test as the pre and post tests and Oxford Placement Test (OPT) in order to obtain the homogeneity in participants’ English proficiency. The collected data were codified and entered into SPSS Software (Version 22) and were analysed using descriptive statistics, t-test, and Tukey test. The results indicated that although applying these two types of corrective feedback could have made improvement in EFL learners’ speaking ability, there was not observed any significant difference between impacts of recast and metalinguistic on EFL learners’ production. The test results also indicated that there was not any significant difference regarding gender within the three groups. This homogeneity further shows that in this study, the gender variable did not have any effect on the role of corrective feedback and it can be concluded that the observed difference between metalinguistic group, recast group, and control group is just the result of the provided corrective feedback type which has acted as the intervening variable and the moderator variable such as gender did not prove to have any effect in the outcome of this study. The findings can contribute to syllabus design and teaching methodology areas.</p>

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Studying the Role of C-Reactive Protein in Patients with Sleeve Gastrectomy in Baghdad

Journal of Techniques ◽

10.51173/jt.v3i1.282 ◽

2021 ◽

Vol 3 (1) ◽

pp. 55-60

Author(s):

Mohammed Abbas Fadil ◽

Raya Ezat Maroof ◽

Moayed Abbas Fadil

Keyword(s):

Sleeve Gastrectomy ◽

Healthy Person ◽

Control Group ◽

C Reactive Protein ◽

Serum Samples ◽

Reactive Protein ◽

Medical City ◽

Healthy Control ◽

And Control

Obesity and severe obesity are increasing serious health problems with an epidemic percentage in most countries. In Sleeve gastrectomy, a part of the stomach structure is removed, limiting its capacity by about two to three. A total of thirty blood samples were collected from patients with obesity and the result was compared with healthy person throughout the time from November 2019 to September 2020. Before sleeve gastrectomy and after more than 6 months of sleeve surgery, the sample was collected from the Medical City/Baghdad Teaching Hospital, the withdrawal was again taken at home to have pre and post sleeve gastrectomy, Patient age ranged between [20-46] years for obese patients and healthy control. Then the serum samples were obtained from patients and control group to screen for C-reactive protein by agglutination method. The result of the present study found that the positivity of CRP in pre-operation is higher than that of post-operative with high significance [P<0.005].

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Effect of Probiotics on Allergic Rhinitis: A Randomized, Controlled, Clinical Trial

Galen Medical Journal ◽

10.31661/gmj.v9i0.1918 ◽

2020 ◽

Vol 9 ◽

pp. 1918 ◽

Cited By ~ 1

Author(s):

Mahnaz Sadeghi-Shabestari ◽

Yalda Jabbari Moghaddam ◽

Hasan Rezapoor ◽

Mojataba Sohrabpour

Keyword(s):

Clinical Trial ◽

Allergic Rhinitis ◽

Standard Therapy ◽

Control Group ◽

Controlled Clinical Trial ◽

P Value ◽

Nasal Discharge ◽

Oral Corticosteroids ◽

And Control

Background: Allergic rhinitis (AR) is one of the most common diseases in the world and affects about 10-50% of the general population. Probiotics are live microorganisms that help the normal state of the intestine, and if prescribed correctly, they can stimulate the mucosal immune system to prevent inflammatory symptoms of allergy and atopy. The present study aims to investigate the role of probiotics in the treatment of AR when added to standard therapy as adjuvant agents. Materials and Methods: In this clinical trial study, 28 patients older than 15 years with AR randomly divided into probiotics and control groups. The probiotics group received standard therapy for AR accompanied by probiotic capsules every 12 hours for eight weeks, whereas the control group received standard therapy for AR with placebo capsules as the same protocol. Data were analyzed using SPSS Version 23 (IBM Corporation, Armonk, NY, USA) and, the P-value less than 0.05 was considered statistically significant. Results: In the probiotics group, 14.3% of patients had sneezing at the baseline, which significantly decreased to 4.6% (P<0.01). Also, the necessity for nasal and oral corticosteroids after treatment with probiotics in the probiotics group was less than the control group (P<0.01). Although cough, nasal discharge, conchae hypertrophy, and night sleep disorders reduced after treatment with probiotics, this reduction was not statistically significant between the two groups. Conclusion: Based on the results of this clinical trial, the use of probiotics had no significant effect on the outcome of patients with AR. [GMJ.2020;9:e1918]

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Clinical Impact of Estradiol/Testosterone Ratio in Patients with Acute Ischemic Stroke

10.21203/rs.3.rs-33503/v3 ◽

2020 ◽

Author(s):

Jung-Won Choi ◽

In Woo Ryoo ◽

Jun Yeong Hong ◽

Kyung-Yul Lee ◽

Hyo Suk Nam ◽

...

Keyword(s):

Ischemic Stroke ◽

Acute Ischemic Stroke ◽

Sex Hormones ◽

Control Group ◽

Control Groups ◽

Sexual Hormones ◽

Stroke Group ◽

Male Patients ◽

And Control

Abstract Background: Sex hormones may be associated with a higher incidence of ischemic stroke or stroke-related events. In observational studies, lower testosterone concentrations are associated with infirmity, vascular disease, and adverse cardiovascular risk factors. Currently, female sexual hormones are considered neuroprotective agents. The purpose of this study was to assess the role of sex hormones and the ratio of estradiol/testosterone (E/T) in patients with acute ischemic stroke (AIS).Methods: Between January 2011 and December 2016, 146 male patients with AIS and 152 age- and sex-matched control subjects were included in this study. Sex hormones, including estradiol, progesterone, and testosterone, were evaluated in the AIS patient and control groups. We analyzed the clinical and physiological levels of sex hormones and hormone ratios in these patients.Results: The E/T ratio was significantly elevated among patients in the stroke group compared to those in the control group (P = 0.001). Categorization of data into tertiles revealed that patients with the highest E/T ratio were more likely to have AIS [odds ratio (OR) 3.084; 95% Confidence interval (CI): 1.616-5.886; P < 0.001) compared with those in the first tertile. The E/T ratio was also an independent unfavorable outcome predictor with an adjusted OR of 1.167 (95% CI: 1.053-1.294; P = 0.003).Conclusions: These findings support the hypothesis that increased estradiol and reduced testosterone levels are associated with AIS in men.

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