scholarly journals Effect of Er,Cr:YSGG Laser Irradiation with Radial Firing Tips onCandida albicansin Experimentally Infected Root Canals

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Leman Ozkan ◽  
Serap Cetiner ◽  
Tamer Sanlidag
Keyword(s):  
Morphological Changes ◽  
Irradiation Effect ◽  
Treatment Protocols ◽  
Root Canals ◽  
Group 4 ◽  
Infected Root ◽  
Significant Difference ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

Aim. To compare the disinfection effect of Er,Cr:YSGG laser using radial firing tips with NaOCI in root canals infected withC. albicansand to evaluate the irradiation effect on the dentinal surfaces.Material and Methods. In total seventy-six mandibular premolar teeth were used. In order to standardize the incubation and sterilization procedure, eight teeth were used. Sixty-eight of the root canals were incubated withC. albicanssuspension for 72 hours. The specimens were divided into 5 experimental groups. Two groups were constituted as Group 1 was irradiated with 1.5 W laser (n=8) and group 2, which was irradiated with 2 W laser (n=8). Two more groups were formed as Group 3 (2 W laser (n=25) and Group 4 NaOCI (5%) (n=25). Group 5 (n=2) did not receive any treatment. Mann-WhitneyUand Kruskal-WallisHtests were used to compare the different laser output powers. Wilcoxon Signed Ranks Test was used in order to compare theCandidacfu/ml levels according to treatment protocols (P<0.05).Results. Both 1.5 W and 2 W laser resulted in a major reduction ofC. albicanswithout a significant difference. The comparison of the dentin surfaces irradiated with Er,Cr:YSGG laser at two power settings resulted in similar morphological changes. However, NaOCI was found to be more effective in reduction ofC. albicansthan 2 W laser application.Conclusion. According to the results of the present study, the Er,Cr:YSGG laser with radial firing tips presented less antifungal effects onC. albicansin root canals of infected teeth than NaOCl solution.

Morphological Changes in the Rat Granulosa Cell Surface During Differentiation Induced by Estradiol and Gonadotropins

1977 ◽  
Vol 35 ◽  
pp. 484-485
Author(s):  
P. Bagavandoss ◽  
JoAnne S. Richards ◽  
A. Rees Midgley
Keyword(s):  
Cell Surface ◽  
Granulosa Cell ◽  
Morphological Changes ◽  
Follicular Development ◽  
Female Rats ◽  
Functional Changes ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

During follicular development in the mammalian ovary, several functional changes occur in the granulosa cells in response to steroid hormones and gonadotropins (1,2). In particular, marked changes in the content of membrane-associated receptors for the gonadotropins have been observed (1).We report here scanning electron microscope observations of morphological changes that occur on the granulosa cell surface in response to the administration of estradiol, human follicle stimulating hormone (hFSH), and human chorionic gonadotropin (hCG).Immature female rats that were hypophysectcmized on day 24 of age were treated in the following manner. Group 1: control groups were injected once a day with 0.1 ml phosphate buffered saline (PBS) for 3 days; group 2: estradiol (1.5 mg/0.2 ml propylene glycol) once a day for 3 days; group 3: estradiol for 3 days followed by 2 days of hFSH (1 μg/0.1 ml) twice daily, group 4: same as in group 3; group 5: same as in group 3 with a final injection of hCG (5 IU/0.1 ml) on the fifth day.

In vivosilymarin’s antioxidant and anti-apoptotic effects on photodynamic therapy’s responsiveness

2017 ◽  
Vol 21 (03) ◽  
pp. 189-197 ◽  
Author(s):  
Vlad Dionisie ◽  
Simona Clichici ◽  
Rodica M. Ion ◽  
Oana O. Danila ◽  
Remus Moldovan ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Reactive Oxygen Species Generation ◽  
Group 4 ◽  
Significant Difference ◽  
Anti Oxidant ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Apoptotic Effects ◽  
Oxidant Effect

Several studies have shown that some anti-oxidant natural compounds in combination with photodynamic therapy (PDT) can enhance the effectiveness of treatment. The aim of this study is to evaluate the effect of silymarin (SIL) in combination with 5,10,15,20-tetra-sulphonato-phenyl-porphyrin (TSPP) based photodynamic therapy, on experimental tumors. 30 Wistar rats with Walker carcinosarcoma, were divided into 6 groups: group 0 (control) — control, untreated group; group 1 (TSPP) — one dose of TSPP; group 2 (SIL) — silymarin; group 3 (PDT) — TSPP and irradiation 24 h after; group 4 (SIL[Formula: see text]PDT) — silymarin, TSPP and irradiation 24 h after; group 5 (SIL[Formula: see text]IR) and group 6 (IR) — irradiation and in addition, group 5 received SIL. Silymarin administered before photodynamic therapy decreased the lipid peroxidation ([Formula: see text] < 0.05) and modulated the antioxidant defense in tumor treated with PDT and silymarin suggesting that silymarin administration along with photodynamic therapy has an anti-oxidant effect. The caspase — 8 level and -3 activity increased in PDT and PDT [Formula: see text] SIL groups compared to the control; between the two groups there was a significant difference in term of apoptosis in favor to PDT. In conclusion, silymarin administration inhibited the reactive oxygen species generation and reduced the tumoral cells’ apoptosis, suggesting that natural compound administered before photodynamic therapy did not improve the therapy’s effect.

scholarly journals Evaluation of Apical Filling after Warm Vertical Compaction using Two Different Endodontic Materials: Resilon® and Gutta-Percha

2012 ◽  
Vol 3 (2) ◽  
pp. 166-170
Author(s):  
Carla Zogheib ◽  
Etienne Medioni
Keyword(s):  
Group 4 ◽  
Gutta Percha ◽  
Anterior Teeth ◽  
Significant Difference ◽  
Ah Plus ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Warm Vertical Compaction ◽  
Group 1

ABSTRACT Forty-eight extracted maxillary anterior teeth instrumented with ProTaper system to apical preparations 0.25, 0.40 and 0.60 mm (n = 16 each) were divided into six groups for filling as following: Group 1: Apical preparation 25/100 + gutta-percha/AH Plus sealer, group 2: Apical preparation 25/100 + Resilon/Real Seal, group 3: Apical preparation 40/100 + gutta-percha/AH plus sealer, group 4: Apical preparation 40/100 + Resilon/Real Seal, group 5: Apical preparation 60/100 + gutta-percha/AH plus, group 6: Apical preparation 60/100 + Resilon/Real Seal. All teeth were filled using the warm vertical technique. Horizontal sections at 1, 2 and 3 mm from the apex were observed by SEM under 200 × magnification. Sections were digitally photographed under a stereomicroscope and the images were transferred to a compatible PC for image analysis. The surface area of voids were calculated and compared at all levels; using the Kruskal-Wallis test with Sidak correction and Mann-Whitney U test (p > 0.05). Comparisons within each group (gutta-percha/AH plus and Resilon/Real Seal) showed the presence of voids but there was no significant difference between any level of sectioning for apical preparations of 25 and 40 (p > 0.05). The only difference was in the groups prepared to size 60 and filled with Resilon/ Real Seal which showed significantly more voids than the group filled with gutta-percha/AH plus and especially at the 3 mm level. Conclusion The system Resilon/Real Seal did not achieve better results in terms of sealing ability in the apical third when compared to the conventional gutta-percha/AH plus sealing system. How to cite this article Zogheib C, Naaman A, Medioni E. Evaluation of Apical Filling after Warm Vertical Compaction using Two Different Endodontic Materials: Resilon® and Gutta-Percha. World J Dent 2012;3(2):166-170.

Blood Ca2+ modulates responsiveness of renal 25(OH)D3-1 alpha-hydroxylase to PTH in rats

1987 ◽  
Vol 253 (5) ◽  
pp. E503-E507 ◽  
Author(s):  
T. Matsumoto ◽  
K. Ikeda ◽  
K. Morita ◽  
S. Fukumoto ◽  
H. Takahashi ◽  
...  
Keyword(s):  
Base Line ◽  
Hydroxylase Activity ◽  
Dihydroxyvitamin D ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Serum Inorganic Phosphate ◽  
Group 1

To clarify whether extracellular Ca2+ modulates renal 25-hydroxyvitamin D3 [25(OH)D3]-1 alpha-hydroxylase, thyroparathyroidectomized rats were infused with 15 mM CaCl2, 20 mM EGTA, and/or 2.5 U/h parathyroid hormone (PTH), and blood Ca2+, serum 1,25-dihydroxyvitamin D [1,25(OH)2D], and renal 1 alpha-hydroxylase activity were determined. Rats with CaCl2, EGTA, or PTH infusion (group 1) exhibited low blood Ca2+, serum 1,25(OH)2D, and 1 alpha-hydroxylase activities. Infusion of CaCl2 alone (group 2) caused a significant increase in blood Ca2+ and a reduction in serum 1,25(OH)2D and 1 alpha-hydroxylase compared with group 1. Administration of PTH alone (group 3) markedly elevated blood Ca2+, serum 1,25(OH)2D, and 1 alpha-hydroxylase activity. When EGTA was infused along with PTH (group 4), blood Ca2+ was significantly reduced compared with group 3, and serum 1,25(OH)2D and renal 1 alpha-hydroxylase were further elevated. In contrast, when CaCl2 was infused with PTH (group 5), blood Ca2+ was higher than that in group 3, and serum 1,25(OH)2D and 1 alpha-hydroxylase activities were significantly reduced compared with group 3. No significant difference in serum inorganic phosphate or urinary cAMP excretion was observed by CaCl2 or EGTA infusion in both PTH-treated and nontreated rats. These results demonstrate that extracellular Ca2+ modulates the responsiveness of renal 1 alpha-hydroxylase to PTH as well as the base-line activity of the enzyme in the absence of PTH. These effects of extracellular Ca2+ on renal 1 alpha-hydroxylase may serve to offer an efficient way of regulating 1,25(OH)2D production and serum 1,25(OH)2D concentration by altering the responsiveness of 1 alpha-hydroxylase to PTH and possibly other stimulations depending on the demand for Ca2+.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Feeding preferences of experienced and naïve goats and sheep for the toxic plant Ipomoea carnea subsp. fistulosa

2015 ◽  
Vol 45 (9) ◽  
pp. 1634-1640 ◽  
Author(s):  
Carlos Alberto Oliveira Júnior ◽  
Gabriela Riet-Correa ◽  
Everton Lima ◽  
Danilo Maia Leite ◽  
James A. Pfister ◽  
...  
Keyword(s):  
Cynodon Dactylon ◽  
Feeding Preferences ◽  
Ipomoea Carnea ◽  
Group 4 ◽  
Plant Group ◽  
Significant Difference ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Toxic Plant

<p><bold>Ipomoea carnea</bold> is a toxic plant that grazing goats and cattle may learn to ingest with repeated exposure. The objective of this study was to evaluate the feeding preferences of experienced and non-experienced (naïve) goats and sheep for<bold> I. carnea.</bold>The study used 3 groups of 5 goats (Group 1, experienced that were previously poisoned by the plant; group 2, naïve; Group 3, experienced eaters, composed of animals adapted to eat the fresh plant) and 2 groups of sheep (group 4, experienced that were previously poisoned by the plant; and group 5, naïve). For the test, the animals were placed daily for 10 minutes and 4 days in a rectangular stall (5x7m) with 4 feeders, each with 200g of a different food (<bold>Ipomoea carnea</bold>, commercial concentrate food, recently harvested green grass (mainly <bold>Brachiaria</bold> spp.), and <bold>Cynodon dactylon</bold>hay. The intake of concentrate food was significantly higher (P<0.05) than the consumption of green grass, hay and <bold>I. carnea</bold>. In a second 4 day trial, in which the commercial concentrate food was replaced by freshly harvested <bold>Amorimia septentrionalis</bold>, the ingestion of green grass (<bold>Brachiaria</bold> spp.) was significantly higher (P<0.05) than the consumption of other foods. In both trials there was no significant difference in food consumption between eaters and naïve animals. The results suggest that experienced or naïve sheep and goats do not prefer <bold>I. carnea</bold> when it is offered with other foods or forages, suggesting that animals will avoid the plant and not become poisoned if other food options are available.</p>

Evaluation of the Effect of Different Ferrule Designs on the Fracture Resistance of Endodontically Treated Maxillary Central Incisors Incorporating Fiber Posts, Composite Cores and Crown Restorations

2007 ◽  
Vol 8 (7) ◽  
pp. 62-69 ◽  
Author(s):  
Emre Ozel ◽  
Idil Dikbas ◽  
Jale Tanalp ◽  
Temel Koksal ◽  
Mustafa Ersoy
Keyword(s):  
Fracture Resistance ◽  
Quartz Fiber ◽  
Group 4 ◽  
Fiber Posts ◽  
Significant Difference ◽  
Group 6 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Abstract Aim The purpose of this study was to evaluate the fracture resistance of endodontically treated maxillary central incisors restored with quartz fiber posts, composite cores, and crowns when different types of ferrule designs were incorporated. Methods and Materials Sixty maxillary incisors were divided into six groups: Group 1 (control): teeth with root canal treatments having a full crown prosthesis; Group 2: teeth with a 2 mm circumferential ferrule; Group 3: teeth with a 2 mm ferrule only in the vestibular region; Group 4: teeth with a 2 mm ferrule only in the palatal region; Group 5: teeth with a 2 mm ferrule in the vestibular and palatal region, having cavities in both proximal areas; and Group 6: teeth with no ferrule. The teeth in the experimental groups were restored with quartz fiber posts-composite cores and full metallic crowns. All experimental teeth were subjected to an increasing compressive force with a crosshead speed of 1 mm/min, until fracture occurred. Results The median fracture values of groups were as follows: Group 1: 574.4 N, Group 2: 472.4 N, Group 3: 474.3 N, Group 4: 480.7 N, Group 5: 463.1 N, and Group 6: 297.9 N. A statistically significant difference was found between Group 1 and Group 6 (p< 0.01). Conclusion It was concluded different ferrule designs did not have any influence on the fracture resistance of teeth with fiber posts. The results of this study indicate fiber posts can safely be used for their reinforcing properties. Furthermore, there is no significant change in the resistance of teeth with fiber posts regardless of which ferrule design is incorporated. The property of these types of posts is an additional advantage in clinical practice. Citation Dikbas I, Tanalp J, Ozel E, Koksal T, Ersoy M. Evaluation of the Effect of Different Ferrule Designs on the Fracture Resistance of Endodontically Treated Maxillary Central Incisors Incorporating Fiber Posts, Composite Cores and Crown Restorations. J Contemp Dent Pract 2007 November; (8)7:062-069.

Expression of the Proto-Oncogene eIF4E in Inflammation of the Oral Cavity

2002 ◽  
Vol 126 (3) ◽  
pp. 290-295 ◽  
Author(s):  
Binoy Chandy ◽  
Fleurette Abreo ◽  
Raja Nassar ◽  
Fred J. Stucker ◽  
Cherie-Ann Nathan
Keyword(s):  
Oral Cavity ◽  
Normal Mucosa ◽  
Premalignant Lesions ◽  
Mild Dysplasia ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

OBJECTIVE: eIF4E (4E) is elevated in 100% of head and neck squamous cell carcinoma (HNSCC) and in premalignant lesions of the larynx. However, it is not elevated in normal mucosa. In this study, we hypothesize that 4E is not significantly elevated in inflammation unlike its expression in premalignant lesions of the oral cavity. STUDY DESIGN: Biopsies from the oral cavity were divided into 5 groups: (1) normal mucosa, (2) chronic inflammation, (3) mild dysplasia from leukoplakic lesions, (4) mild dysplasia in surgical margins of patients with HNSCC, and (5) HNSCC. Immunohistochemical qualitative analysis was then performed. RESULTS: None of the 15 specimens in group 1 and 100% of the 15 specimens in group 5 expressed 4E. Of the 29 specimens in group 2 only 4/29 (13%) overexpressed 4E compared with 10/31 (32%) in group 3 and 9/21 (42%) in group 4. There was a significant difference between groups 2 and 3 and groups 2 and 4 ( P < 0.0001 and P < 0.003 respectively) but no significant difference between groups 1 and 2 ( P = 0.13) and between groups 3 and 4 ( P = 0.30). CONCLUSION: 4E is not significantly elevated in inflammation of the oral cavity thus fulfilling one of the criteria that biomarkers require to be useful in a clinical setting.

Physicochemical Analysis of Dental and Household Hypochlorites

2021 ◽  
Author(s):  
Michele Méndez Velázquez
Keyword(s):  
Physicochemical Analysis ◽  
Pulp Tissue ◽  
Common Solution ◽  
Group 4 ◽  
All Solutions ◽  
Significant Difference ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Aim: Sodium hypochlorite is the most common solution in the Endodontics field, however, NaOCl concentrations of commercial brands which are prepared for this purpose differ from their actual concentration. The aim of this study was to compare the physicochemical properties of dental and household hypochlorites. Materials and Methods: The solutions were divided in the following groups: Control: Clorarex 6%, Group 1: Viarzoni-T 5.25%, Group 2: Endo Chlord 3%, Group 3: Chlor- XTRA 6%, Group 4: Viarzoni-T (diluted from 5.25%), Group 5: Cloralex (diluted from 6%), Group 6: Chlor- Xtra (diluted from 6%). pH and temperature were measured by means of potentiometer, dilution test was performed using bovine tissue and exposed to each solution. For cytotoxicity assay, erythrocytes were exposed to each solution and stained with Sternheimer-Malbin solution. Results: Dissolution times showed a significant difference since the shortest time was Cloralex (6.6 ± 2.09). The solution with the highest alkalinity in the original concentration was Endo Chlord for dental use (11.7 ± 2.2). The solution with the highest alkalinity in the original concentration was Endo Chlord for dental use (11.7 ± 2.2) but it also was the solution with less cytotoxicity. Conclusion: Viarzoni-T was the only solution without any dissolving capacity, Cloralex, ChorlXtra and EndoChlord were capable of dissolving bovine pulp tissue effectively, reducing their concentration of Cloralex and ChlorXtra did not reduce the pH, but all solutions showed a cytotoxic effect on erythrocites.

scholarly journals COMPARATIVE EVALUATION OF THE EFFECT OF SMEAR LAYER REMOVAL WITH EDTA AND ND: YAG LASER ON THE APICAL MICRO LEAKAGE OF TWO EPOXY RESIN BASED ROOT CANAL SEALERS - AN INVITRO STUDY.

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Dhanalakshmi Subramanian ◽  
Mahendran Kavitha ◽  
Anuraag Gurtu ◽  
Bakthavatchalam Balakrishnan ◽  
Mahalakshmi Jayaraman
Keyword(s):  
Nd:Yag Laser ◽  
Smear Layer ◽  
Maxillary Central Incisor ◽  
Layer Removal ◽  
Group 4 ◽  
Significant Difference ◽  
Ah Plus ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

Aim: To compare the effect of smear layer removal with 17% EDTA and Nd:YAG laser on the apical microleakage of two resin based sealers. Materials and Methods: Sixty freshly extracted maxillary central incisor teeth with patent canals were selected. The teeth were debrided and stored in saline for 24 hours before use. The teeth were sectioned at CEJ & cleaning and shaping were done upto 50 size by step back technique. The following groups were analyzed. GROUP 1:  Gutta percha (GP) + AH plus sealer without smear layer removal. GROUP 2:  GP + RC seal sealer without smear layer removal. GROUP 3:  GP + AH plus treated with 17% EDTA. GROUP 4:  GP + RC seal treated with 17% EDTA. GROUP 5:  GP + AH plus treated with Nd:YAG laser. GROUP 6:  GP + RC seal treated with Nd:YAG laser. 8 samples were subjected to dye penetration study for apical microleakage & 2 samples were subjected to SEM in each group to show the effect of smear layer removal by EDTA and laser. The data were analyzed by using ANOVA and TUKEY-HSD test. Results: Groups 1 and 2 showed maximum microleakage , other groups showed lesser microleakage but there was no statistically significant difference between laser and EDTA treated Groups.  Conclusion: EDTA and Nd:YAG laser can be effectively used to  remove smear layer and showed less apical microleakage compared to non-treated groups. Keywords: 17% EDTA, Nd:YAG LASER , AH plus , RC sealer ,  Smear layer.

scholarly journals Effect of spreader size on microleakage of roots filled with cold lateral compaction technique

2015 ◽  
Vol 09 (03) ◽  
pp. 378-381
Author(s):  
Tugba Turk ◽  
Beyser Piskin ◽  
Hasan Orucoglu ◽  
Berdan Aydin
Keyword(s):  
Maxillary Incisor ◽  
Human Teeth ◽  
Fluid Filtration ◽  
Root Canals ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Apical Leakage ◽  
Group 1

ABSTRACT Objectives: To evaluate the effect of spreader size on apical leakage of maxillary incisor teeth. Materials and Methods: A total of 75 permanent human teeth with no carious and no fracture or crack were used for this study. After removing the crown from the cementoenamel junction and the standardization of the root lengths, the specimens were randomly divided into five groups: Group 1 - Roots were not instrumented. Group 2 - Root canals were enlarged using the step-back technique to a #40 file and filled using cold lateral compaction (CLC) of gutta-percha (GP). Group 3 - During the filling procedure, the first spreader used was size 40. Group 4 - The first spreader used was size 35. Group 5 - The initial spreader used was size 25. The amount of leakage through the filled root canals was evaluated by computerized fluid filtration model. Statistical analyzes were done using Kruskal–Wallis test and Mann–Whitney test (P < 0.05). Results: There were statistically significant differences among the groups (P < 0.05). While the uninstrumented group (Group 1) had no leakage, instrumented but not filled roots (Group 2) demonstrated the highest leakage values. There were no differences between Group 3 and 4. Group 5 showed significantly less leakage than Group 3 and 4. Conclusion: Spreader size used during CLC of GP appeared to be a significant factor on apical leakage of roots. Using smaller size spreader during CLC may provide relatively less leakage.

Sign in / Sign up

Export Citation Format

Share Document