scholarly journals Clinical Presentation and Antibiotic Susceptibility of Contact Lens Associated Microbial Keratitis

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Hesam Hedayati ◽  
Mahboubeh Ghaderpanah ◽  
Seyed Ahmad Rasoulinejad ◽  
Mohammad Montazeri
Keyword(s):  
Contact Lens ◽  
Antibiotic Susceptibility ◽  
Clinical Presentation ◽  
Causative Organism ◽  
Microbial Culture ◽  
Microbial Keratitis ◽  
Cross Sectional ◽  
Corneal Ulcers ◽  
Contact Lens Solution ◽  
Southwest Of Iran

Introduction.In recent years, the number of contact lens wearers has dramatically increased in Iran, particularly in youngsters. The purpose of current study was to assess the clinical presentation and antibiotic susceptibility of contact lens related microbial keratitis in Ahvaz, southwest of Iran.Methodology.A cross-sectional investigation of 26 patients (33 eyes) with contact lens induced corneal ulcers who were admitted to Imam Khomeini Hospital, Ahwaz City, from June 2012 to June 2013 was done. In order to study microbial culture and susceptibility of corneal ulcers, all of them were scraped.Results.Eight samples were reported as sterile.Pseudomonas aeruginosa(80%) in positive cultures was the most widely recognized causative organism isolated. This is followed byStaphylococcus aureus12% andEnterobacter8%. The results showed that 84% of the microorganism cases were sensitive to ciprofloxacin, while imipenem, meropenem, and ceftazidime were the second most effective antibiotics (76%).Conclusion.Results of current study show the importance of referring all contact lens wearers with suspected corneal infection to ophthalmologists for more cure. The corneal scraping culture and contact lens solution should be performed to guide antibiotic therapy.

Relationship Between Climate, Disease Severity, and Causative Organism for Contact Lens–Associated Microbial Keratitis in Australia

2007 ◽  
Vol 144 (5) ◽  
pp. 690-698.e1 ◽  
Author(s):  
Fiona Stapleton ◽  
Lisa J. Keay ◽  
Paul G. Sanfilippo ◽  
Suchi Katiyar ◽  
Katie P. Edwards ◽  
...  
Keyword(s):  
Disease Severity ◽  
Contact Lens ◽  
Causative Organism ◽  
Microbial Keratitis

scholarly journals Histopathology of Cutaneous Leishmaniasis Caused by Leishmania donovani in Sri Lanka

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Harshima Wijesinghe ◽  
Nayana Gunathilaka ◽  
Saveen Semege ◽  
Nishantha Pathirana ◽  
Nuwani Manamperi ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Cutaneous Leishmaniasis ◽  
Leishmania Donovani ◽  
Clinical Presentation ◽  
Cross Sectional Study ◽  
Causative Organism ◽  
Variable Intensity ◽  
Cross Sectional ◽  
The North ◽  
Parasitic Load

Cutaneous leishmaniasis (CL) is a neglected tropical disease that is gaining importance in Sri Lanka and internationally. The clinical presentation, pathology, and method of parasite elimination in CL vary according to the species. Leishmania donovani is the causative organism for leishmaniasis in Sri Lanka. This collaborative cross-sectional study describes the clinicopathological features of cutaneous leishmaniasis among personnel of the tri-forces serving in the North and East of the country. The histology of fifty cases of CL confirmed by at least two methods (slit skin smear, lesion aspirate, tissue impression, and histology) was reviewed. The parasitic load was assessed semiquantitatively. The histological features were correlated with the clinical presentation and organism load. The majority (89.8%; n=44) presented with a single lesion mostly located in the upper limb (69.4%). The lesion types included papule (34.7%), nodule (32.7%), and an ulcer (30.6%). The evolution time of lesions averaged 31.55 weeks. Epidermal changes were observed in 49 of the biopsies and included hyperkeratosis (90.0%; n=45), acanthosis (44.0%; n=22), atrophy (34.0%; n=17), and interface change (66%; n=33). Dermal changes were seen in all cases and were characterized by a lymphohistioplasmacytic inflammatory infiltrate of variable intensity with ill-formed granuloma in 19 cases (38%) and well-formed epithelioid granulomas in 22 cases (44%). Focal necrosis was present in 20% (n=10). Leishmania amastigote forms were observed in 88% (n=44). Transepidermal elimination (P=0.025), granuloma (P=0.027) formation, and type of lesion (P=0.034) were significantly associated with the organism load. Granuloma formation was associated with a reduction in organism load, indicating that the macrophage activation played an important role in the control of the organism.

scholarly journals Component Analysis of Multipurpose Contact Lens Solutions To Enhance Activity against Pseudomonas aeruginosa and Staphylococcus aureus

2016 ◽  
Vol 60 (7) ◽  
pp. 4259-4263 ◽  
Author(s):  
Leo Lin ◽  
Janie Kim ◽  
Hope Chen ◽  
Regis Kowalski ◽  
Victor Nizet
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Contact Lens ◽  
Dilution Method ◽  
Microbial Keratitis ◽  
Content Type ◽  
Contact Lens Solutions ◽  
Contact Lens Solution ◽  
Broth Dilution ◽  
Solution Formulation

ABSTRACTMore than 125 million people wear contact lenses worldwide, and contact lens use is the single greatest risk factor for developing microbial keratitis. We tested the antibacterial activity of multipurpose contact lens solutions and their individual component preservatives against the two most common pathogens causing bacterial keratitis,Pseudomonas aeruginosaandStaphylococcus aureus. Thein vitroantibacterial activity of five multipurpose contact lens solutions (Opti-Free GP, Boston Simplus, Boston Advance, Menicare GP, and Lobob) was assayed by the standard broth dilution method. Synergy between the preservative components found in the top performing solutions was assayed using checkerboard and time-kill assays. The ISO 14729 criteria and the standard broth dilution method were used to define an optimized contact lens solution formulation against a clinical panel of drug-susceptible and drug-resistantP. aeruginosaandS. aureusstrains. Preservatives with the biguanide function group, chlorhexidine and polyaminopropylbiguanide (PAPB), had the best antistaphylococcal activity, while EDTA was the best antipseudomonal preservative. The combination of chlorhexidine and EDTA had excellent synergy againstP. aeruginosa. A solution formulation containing chlorhexidine (30 ppm), PAPB (5 ppm), and EDTA (5,000 ppm) had three to seven times more antipseudomonal activity than anything available to consumers today. A multipurpose contact lens solution containing a combination of chlorhexidine, PAPB, and EDTA could help to reduce the incidence of microbial keratitis for contact lens users worldwide.

scholarly journals Microbiological analysis of contact lens cases and effective health behaviors

2020 ◽  
Vol 7 (2) ◽  
pp. e17-e17
Author(s):  
Fatemeh Eslami ◽  
Hamid Reza Ghasemi Basir ◽  
Abbas Moradi ◽  
Mina Bayat
Keyword(s):  
Health Behaviors ◽  
Contact Lens ◽  
Bacterial Contamination ◽  
Contact Lenses ◽  
Significant Proportion ◽  
Enterobacter Aerogenes ◽  
Cross Sectional Study ◽  
Microbial Culture ◽  
Microbiological Analysis ◽  
Cross Sectional

Introduction: Contact lenses are increasingly being used for cosmetic or therapeutic purposes, followed by subsequent contamination and complications such as keratitis. The lens case is one of the most common places to find the cause of contamination. Objectives: This study was designed to evaluate the health behaviors affecting the lens case contamination and its relationship with the result of lens case culture which can help in prevention of complications. Patients and Methods: In this cross-sectional study that was performed in northwest of Iran, 150 asymptomatic participants were assessed for health behaviors affecting the lens case contamination and their lens cases were sampled for culture and antibiogram. Data were analyzed with SPSS-16 software. Results: The frequency of positive microbial culture in medical and cosmetic contact lens cases was 30.7% and 66.8%, respectively and 32.7% in general. Among the isolated bacteria observed in positive cultures, Alcaligenes,Enterobacter aerogenes, gram-positive Diphtheroid bacilli, Pseudomonas aeruginosa and Staphylococcus epidermidis were the most common microorganisms, respectively. Conclusion: A significant proportion of contact lens cases, especially those used for cosmetic purposes had bacterial contamination. Failure to replace the lens case for more than 9 months and the mismatch of the lens solution brand with its storage case will increase bacterial contamination. Washing the lens case with soap and water, and drying it after washing, will reduce bacterial contamination.

Clinical presentation and morbidity of contact lens–associated microbial keratitis: a retrospective study

2013 ◽  
Vol 252 (2) ◽  
pp. 299-306 ◽  
Author(s):  
Johan G. Hoddenbach ◽  
Sharmila S. Boekhoorn ◽  
Rene Wubbels ◽  
Willem Vreugdenhil ◽  
Jeroen Van Rooij ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Contact Lens ◽  
Clinical Presentation ◽  
Microbial Keratitis

scholarly journals Organism Isolation in Corneal Ulcer- Utility of Different Techniques

2012 ◽  
Vol 52 (185) ◽  
Author(s):  
M Thapa ◽  
A K Sharma ◽  
D N Shah ◽  
S KC ◽  
S Shrestha
Keyword(s):  
Corneal Ulcer ◽  
Descriptive Study ◽  
Microbial Keratitis ◽  
Cross Sectional ◽  
Corneal Ulcers ◽  
Isolation Techniques ◽  
Corneal Scraping ◽  
Microbial Agents ◽  
Culture Negative ◽  
Corneal Biopsy

Introduction: Various techniques are available for isolation of microorganism in cases of microbial keratitis including conventional methods of scraping, re-scraping inoculation in different media and corneal biopsy. Methods: This was a cross sectional descriptive study conducted from February 2002 to July 2003. A total of 161 eyes of corneal ulcers were evaluated with scraping inoculation. Re-scraping and corneal biopsy were performed in those cases which did not grow any organism in the first scraping or which did not respond to initial treatment. Results: Microorganisms were isolated in 116 patients (72.04%) after first scraping. S pneumoniae was seen in 50(42%), Fusarium in 21(18%) and Aspergillus in 16(14%) cases. Re-scraping was done in 22 culture negative and non improving cases of which 14 (63.63%) showed isolation of organisms. After re-scraping, Fusarium and Aspergillus were isolated in four patients each (18% each) followed by S. pneumoniae in 3 (14%), S. aureus in 2 (9%), and Penicillium in 1(5%) case.Corneal biopsy was done in 10 cases with isolation of microbial agents in 7 (70%) with Aspergillus and S. aureus in three each, and S. epidermidis in 1(10%). Conclusions: Apart from conventional scraping, other isolation techniques should be considered in the treatment of non-responding and culture negative cases of corneal ulcers. Compared to bacteria, fungi are difficult to isolate in the first scraping and requires more frequent repeat scraping and corneal biopsy. Keywords: Corneal biopsy, corneal scraping, corneal ulcer, microbial keratitis, organismal isolation.

scholarly journals Antibiotic susceptibility patterns of pseudomonas corneal ulcers in contact lens wearers

2011 ◽  
Vol 18 (3) ◽  
pp. 228 ◽  
Author(s):  
Zahra Mohajernezhadfard ◽  
Payman Vahedi ◽  
Mehrdad Mohammadpour ◽  
Alireza Khodabande
Keyword(s):  
Contact Lens ◽  
Antibiotic Susceptibility ◽  
Corneal Ulcers ◽  
Antibiotic Susceptibility Patterns ◽  
Susceptibility Patterns

scholarly journals Spectral and Antibiotic Susceptibility of Pathogens Isolated from Saudi Patients with Diabetic Foot Infections

2021 ◽  
Vol 12 (1) ◽  
pp. 16-20
Author(s):  
Samiah Hamad S Al-Mijalli
Keyword(s):  
Staphylococcus Aureus ◽  
Diabetic Foot ◽  
Antibiotic Susceptibility ◽  
Empirical Therapy ◽  
Multidrug Resistant ◽  
Foot Ulcers ◽  
Sensitivity Testing ◽  
Cross Sectional ◽  
Diabetic Foot Infections ◽  
Microbiological Methods

Diabetic foot infections (DFIs) are a significant health issue and a common complication among patients with diabetes. To develop antibiotic therapy for these high-risk patients, the current study evaluates the scope of DFIs and identifies the causing microbes. It also measures spectrum and antibiotic susceptibility of the pathogens isolated from adults with DFIs in Saudi Arabia. To achieve the study objectives, a cross-sectional study was implemented and the baseline characteristics for 44 patients with DFIs were defined. Optimal aerobic and anaerobic microbiological techniques were utilized to culture specimens isolated from infected foot ulcers. The standard microbiological methods were employed to identify the bacterial isolates and antibiotic susceptibility testing was conducted following the procedures of the Clinical and Laboratory Standards Institute (CLSI). Results showed that 12 microorganisms were isolated from the participants’ diabetic foot ulcers. Staphylococcus Aureus was ranked first because it appeared in 29 (65.9%) cases. Streptococcus Agalactiae was ranked second and multi-microbial infections were also found. Most of the organisms were susceptible to Vancomycin, Ciprofloxacin, and Cefalexin, but they were resistant to Methicillin, Gentamicin, and Ampicillin antibiotics. Staphylococcus Aureus was most sensitive to Ciprofloxacin, while it was resistant to Methicillin. About 10% of the isolates were multidrug-resistant. The study concludes that while Vancomycin should be used empirically for Gram-positive isolates, Ciprofloxacin can be taken into consideration for most of the Gram-negatives aerobes. Based on including various microorganisms and the advent of multidrug-resistant strains, proper culture and sensitivity testing are necessary prior to the empirical therapy.

Sign in / Sign up

Export Citation Format

Share Document