Association of a Large Panel of Cytokine Gene Polymorphisms with Complications and Comorbidities in Type 2 Diabetes Patients

Aims. The polymorphisms of pro- and anti-inflammatory cytokines may be involved in type 2 diabetes (T2D) pathogenesis and its complications.Methods. We investigated in 102 T2D patients the association of the cytokine polymorphisms in the TNF-α, IL-10, IL-6, TGF-β1, and IFN-γgenes with the T2D microvascular complications and comorbidities (hypertension, dyslipidemia, and obesity). Cytokine genotypes were determined by PCR using Cytokine Genotyping Tray kit.Results.Diabetic retinopathy was associated with GG genotype and G allele in TGF-β1 codon 25C/G polymorphism (p=0.004andp=0.018) and the nephropathy was associated the lower frequency of GG genotype in IL-10 -1082G/A polymorphism (p=0.049). Hypertension was associated with the CC genotype and C allele for IL-10 -592C/A polymorphism (p=0.013andp=0.009) and higher frequencies of T (p=0.047) and C (p=0.033) alleles of the TGF-β1 codon 10T/C and IL-10 -819T/C polymorphisms, respectively. The TGF-β1 codon 10T/C polymorphism was associated with the BMI groups (p=0.026): the CC genotype was more frequent in the group with BMI < 25 Kg/m2, while the TC genotype was more frequent in the group with BMI = 30 Kg/m2.Conclusions. Our findings suggest that TGF-β1 and IL-10 polymorphisms are involved in complications and comorbidities in T2D patients.

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PO086 THE ASSOCIATION BETWEEN FNDC5 GENE POLYMORPHISMS AND DIABETIC RETINOPATHY IN CHINESE TYPE 2 DIABETES PATIENTS

Diabetes Research and Clinical Practice ◽

10.1016/s0168-8227(14)70381-x ◽

2014 ◽

Vol 106 ◽

pp. S89

Author(s):

S.Y. Wang ◽

S.S. Tang ◽

C. Hu ◽

W.P. Jia

Keyword(s):

Type 2 Diabetes ◽

Diabetic Retinopathy ◽

Gene Polymorphisms ◽

Diabetes Patients

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Association of IL-10 (− 1082 A/G) and IL-6 (− 174 G/C) gene polymorphism with type 2 diabetes mellitus in Ethiopia population

BMC Endocrine Disorders ◽

10.1186/s12902-021-00738-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Birhanu Ayelign ◽

Markos Negash ◽

Henok Andualem ◽

Tadelo Wondemagegn ◽

Eyuel Kassa ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Gene Polymorphism ◽

Gene Polymorphisms ◽

Outcome Variable ◽

Cytokine Gene ◽

Increased Risk ◽

Cytokine Gene Polymorphisms

Abstract Background Interleukin (IL)-6 and IL-10 are the most important cytokine with pro and anti-inflammatory activities, respectively. Dysregulation of IL-6 and IL-10 are associated with increased risk of developing Type 2 Diabetes Mellitus (T2DM). Despite this, a fundamental understanding of both cytokine gene polymorphisms with its expression is critical in understanding of cellular mechanism of insulin resistance as well as T2DM intervention. Therefore, this study aimed to assess IL-6 (− 174 G/C) and IL-10 (− 1082 A/G) gene polymorphism, and its association with T2DM, North West Ethiopia. Methods A comparative cross-sectional study from January to May 2018 was conducted on study participants with T2DM and apparently healthy controls. Deoxyribonucleic acid (DNA) extraction and genotyping was carried out by using amplification refractory mutation system polymerase chain reaction to detect polymorphism of IL-6 and IL-10 gene at the position − 174 and − 1082, respectively. The logistic regression model was fitted to assess the association of between cytokine gene polymorphisms and T2DM. Odds ratio with 95% CI was determined to assess the presence and strength of association between the explanatory variables and outcome variable. A P-value < 0.05 was considered as statistically significant. Result Participants carrying the GG genotype of IL-6 (− 174) (OR (95% CI) = 4.61 (2.07–10.54) was a high likelihood of having T2DM compared to those carrying the CC and AA genotypes. AA and AG genotypes of IL-10 (− 1082) were at lower odd of developing T2DM compared to those carrying the GG genotype. In addition, individuals carrying the G allele of IL-6 (− 174) have 2.82-fold odds of developing T2DM compared to individuals carrying the C allele (OR (95% CI) =2.81 (1.78–4.50)). Conclusion Our study revealed that genetic polymorphisms of IL-6 (− 174) GG genotype is the potential host genetic risk factors to T2DM. While, IL-10 (− 1082) AA genotype is negatively associated with T2DM. Therefore, IL-6 (− 174) and IL-10 (− 1082) genetic variation may be considered as a biomarker for early screening and diagnosis of T2DM.

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Correction: Prevalence and Progression Rate of Diabetic Retinopathy in Type 2 Diabetes Patients in Correlation with the Duration of Diabetes

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/a-0757-3796 ◽

2018 ◽

Vol 126 (09) ◽

pp. e2-e2

Author(s):

Margarete Voigt ◽

Sebastian Schmidt ◽

Thomas Lehmann ◽

Benjamin Köhler ◽

Christof Kloos ◽

...

Keyword(s):

Type 2 Diabetes ◽

Diabetic Retinopathy ◽

Progression Rate ◽

Duration Of Diabetes ◽

Diabetes Patients

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MON-187 The E23K Missense Variant of the Gene Encoding the Pancreatic Beta-Cell KATP Channel Subunit Kir6.2 (KCNJ11) Associates with Diabetic Retinopathy and Hyperglycemia Control in Type 2 Diabetes Patients

Journal of the Endocrine Society ◽

10.1210/js.2019-mon-187 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Sameh Sarray ◽

Wassim Almawi

Keyword(s):

Type 2 Diabetes ◽

Diabetic Retinopathy ◽

Beta Cell ◽

Katp Channel ◽

Pancreatic Beta Cell ◽

Missense Variant ◽

Gene Encoding ◽

Diabetes Patients

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STUDY OF PREVALENCE AND CLINICAL PROFILE OF NEPHROPATHY AND RETINOPATHY IN TYPE 2 DIABETES PATIENTS AT TERTIARY CARE CENTER, UDAIPUR

10.36106/gjra/0100380 ◽

2021 ◽

pp. 6-8

Author(s):

Yash Salil Patel

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Diabetic Retinopathy ◽

Diabetic Nephropathy ◽

Microvascular Complications ◽

Tertiary Care ◽

Positive Family History ◽

Diabetic Patients

Microvascular complications of Type 2 Diabetes Mellitus (T2DM), (retinopathy and nephropathy) have a similar etiopathogenetic mechanism besides genetic predisposition. Even though these two complications frequently co-exist, their frequency varies. The association of these two signicant complications and their coexistence needs a relook. To study prevalence of retinopathy and nephropathy in Type 2 diabetes mel Aim: litus. Comparison of diabetic retinopathy and nephropathy in Type 2 diabetes mellitus and its correlation of diabetic retinopathy and nephropathy with duration of illness and various risk factors that affects development, progression and severity of diabetic retinopathy and nephropathy. 100 diabetic patients were taken up for study for a period of one year meeti Methodology: ng the criteria for the present study. Detailed history was taken from patient and meticulous examination was done of all patients with special emphasis on renal and ophthalmic symptoms. Clinical data and investigation prole was tabulated. Statistical analysis was done. Among 100 patients, 22 had diabetic retinopathy. Among patients with diab Results & Conclusion: etic retinopathy, 68.18% patients had positive family history. Among 100 patients, 32 had diabetic nephropathy, mean FBS was 207 mg%, PPBS was 317.8 mg% and mean HbA was 9.2%. Among patients with diabetic retinopathy, mean FBS was 211 mg%, PPBS was 324.9 1c mg%, HbA was 9.5%. From this study it is found that diabetic nephropathy starts earlier than retinopathy. In this study 1c hypertension was found to accelerate progression into nephropathy and retinopathy.

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The Relationship between Peripheral Nerve Conduction Velocity and Ophthalmological Findings in Type 2 Diabetes Patients with Early Diabetic Retinopathy

Journal of Ophthalmology ◽

10.1155/2018/2439691 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Azusa Ito ◽

Hiroshi Kunikata ◽

Masayuki Yasuda ◽

Shojiro Sawada ◽

Keiichi Kondo ◽

...

Keyword(s):

Type 2 Diabetes ◽

Diabetic Retinopathy ◽

Conduction Velocity ◽

Nerve Conduction ◽

Nerve Conduction Velocity ◽

Neuronal Damage ◽

Intima Media Thickness ◽

Clinical Findings ◽

Diabetes Patients

Purpose. Nerve conduction velocity (NCV) is an indicator of neuronal damage in the distal segment of the peripheral nerves. Here, we determined the association between NCV and other systemic and ocular clinical findings, in type 2 diabetes patients with early diabetic retinopathy (DR). Methods. This study included 42 eyes of 42 type 2 diabetes patients (median age: 54 years) with no DR or with mild nonproliferative DR. Standard statistical techniques were used to determine associations between clinical findings. Results. Sural sensory conduction velocity (SCV) and tibial motor conduction velocity (MCV) were significantly lower in mild nonproliferative DR patients than patients with no DR (P=0.008 and P=0.01, resp.). Furthermore, logistic regression analyses revealed that sural SCV and tibial MCV were independent factors contributing to the presence of mild nonproliferative DR (OR 0.83, P=0.012 and OR 0.69 P=0.02, resp.). Tibial MCV was correlated with choroidal thickness (CT) (P=0.01), and a multiple regression analysis revealed that age, tibial MCV, and carotid intima-media thickness were independent associating factors with CT (P=0.035, P=0.015, and P=0.008, resp.). Conclusions. Our findings suggest that reduced NCV may be closely associated with early DR in type 2 diabetes patients. Thus, reduced nerve conduction is a potential early biomarker of DR.

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