scholarly journals High Levels of 17β-Estradiol Are Associated with Increased Matrix Metalloproteinase-2 and Metalloproteinase-9 Activity in Tears of Postmenopausal Women with Dry Eye

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Guanglin Shen ◽  
Xiaoping Ma
Keyword(s):  
Matrix Metalloproteinase ◽  
Mrna Expression ◽  
Postmenopausal Women ◽  
Dry Eye ◽  
Sex Steroids ◽  
Serum Levels ◽  
Matrix Metalloproteinase 2 ◽  
Schirmer Test ◽  
Metalloproteinase 9 ◽  
Estradiol Levels

Purpose. To determine the serum levels of sex steroids and tear matrix metalloproteinases (MMP) 2 and 9 concentrations in postmenopausal women with dry eye.Methods. Forty-four postmenopausal women with dry eye and 22 asymptomatic controls were enrolled. Blood was drawn and analyzed for serum levels of sex steroids and lipids. Then, the following tests were performed: tear collection, Ocular Surface Disease Index (OSDI) questionnaire, fluorescein tear film break-up time (TBUT), corneal fluorescein staining, Schirmer test, and conjunctival impression cytology. The conjunctival mRNA expression and tear concentrations of MMP-2 and MMP-9 were measured.Results. Serum 17β-estradiol levels were significantly higher in the dry eye subjects than in the controls (P=0.03), whereas there were no significant differences in levels of testosterone, dehydroepiandrosterone sulfate (DHEA-S), and progesterone. Tear MMP-2 and MMP-9 concentrations (P<0.001), as well as the MMP-9 mRNA expression in conjunctival samples (P=0.02), were significantly higher in dry eye subjects than in controls. Serum 17β-estradiol levels were positively correlated with tear MMP-2 and MMP-9 concentrations and negatively correlated with Schirmer test values.Conclusions. High levels of 17β-estradiol are associated with increased matrix metalloproteinase-2 and metalloproteinase-9 activity in tears of postmenopausal women with dry eye.

scholarly journals Effects of menopause on corneal topography and dry eye

2017 ◽  
Vol 6 (2) ◽  
pp. 461
Author(s):  
Fethiye Gulden Turgut ◽  
Abdulkadir Turgut ◽  
Zehra Nihal Dolgun ◽  
Nadiye Köroğlu
Keyword(s):  
Postmenopausal Women ◽  
Dry Eye ◽  
Test Scores ◽  
Central Corneal Thickness ◽  
Corneal Thickness ◽  
Schirmer Test ◽  
Positive Correlation ◽  
Eye Symptoms ◽  
Estradiol Levels ◽  
Dry Eye Symptoms

Background: The aim of this study was to investigate the effect of estrogen and progesterone on central corneal thickness, corneal curvature, and the development of dry eye in postmenopausal women.Methods: This is a case-control study including 54 premenopausal and 54 postmenopausal women. The two groups were compared in terms of follicle stimulating hormone, estradiol, progesterone, and horizontal and vertical curvatures, astigmatism, central corneal thickness, Schirmer I test scores, and dry eye scores of both eyes.Results: In the postmenopausal women, central corneal thickness was significantly thinner in both eyes (p=0.017) and the Schirmer test scores were significantly lower both for the right (p=0.001) and the left eye (p=0.003). Estradiol levels were positively correlated with central corneal thickness (p=0.003-0.006) and Schirmer test scores (p=0.004-0.002) whereas progesterone levels showed a positive correlation only with Schirmer test scores (p=0.036-0.044). Although a significant positive correlation was found between estradiol levels and dry eye scores (p=0,009), no significant correlation was found between progesterone levels and dry eye scores (p=0,118).Conclusions: Age-related hormonal abnormalities lead to central corneal thinning, increased dry eye symptoms, and decreased tear flow, particularly in the postmenopausal term. Decreased estrogen levels are associated with central corneal thickness and dry-eye symptoms, decreased estrogen and progesterone levels correlates well with diminished tear production but progesterone levels does not effect dry-eye symptoms.

Matrix metalloproteinase-9 and matrix metalloproteinase-2 as biomarkers of various courses in multiple sclerosis

2009 ◽  
Vol 15 (3) ◽  
pp. 316-322 ◽  
Author(s):  
Y Benešová ◽  
A Vašků ◽  
H Novotná ◽  
J Litzman ◽  
P Štourač ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Matrix Metalloproteinase ◽  
Clinical Status ◽  
Serum Levels ◽  
Matrix Metalloproteinase 9 ◽  
Matrix Metalloproteinase 2 ◽  
Significant Elevation ◽  
Blood Brain Barrier Disruption ◽  
Mcdonald Criteria ◽  
Metalloproteinase 9

Background Matrix metalloproteinases are notable contributors to neuroinflammation and blood-brain barrier disruption in multiple sclerosis (MS). Objective The goal of this study was to determine the serum levels of matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-2 (MMP-2), and their tissue inhibitors (TIMP-1) and (TIMP-2), and to investigate their possible relations to type, disability, and severity of MS. Materials and methods Eighty-seven patients with definite MS according to the McDonald criteria and 50 healthy controls were enrolled in the study. Their clinical status was evaluated with the Expanded Disability Status Scale. Serum levels were analyzed by enzyme-linked immunoassay. Results A significant elevation in MMP-9 serum levels and in the MMP-9/TIMP-1 ratio was found in the whole MS group ( P < 0.001), in the relapsing–remitting MS (RRMS) ( P < 0.001), and secondary-progressive MS (SPMS) ( P < 0.001) groups when compared with the controls. A significant elevation in MMP-2 serum levels and in the MMP-2/TIMP-2 ratio was observed in the primary progressive ( P < 0.001) and the SPMS ( P < 0.002) groups when compared with the RRMS group, and this increase was also associated with the disability ( P < 0.001) and severity ( P < 0.05) of the disease. Conclusion We confirmed that metalloproteinases are useful biological markers in MS, providing information about the clinical type, disability, and severity of the disease.

scholarly journals Pathophysiological disturbances in complicated pancreatic pseudocysts

2015 ◽  
Vol 96 (2) ◽  
pp. 191-197
Author(s):  
O Yu Gerbali ◽  
I A Krivoruchko ◽  
N N Goncharova
Keyword(s):  
Matrix Metalloproteinase ◽  
Interleukin 6 ◽  
Serum Levels ◽  
Matrix Metalloproteinase 2 ◽  
Pancreatic Pseudocysts ◽  
Type I ◽  
Tissue Inhibitor ◽  
Favorable Conditions ◽  
Endothelial Growth Factor ◽  
Metalloproteinase 9

Aim. To determine profibrogenic mediators, markers of endothelial dysfunction and hemostasis in patients with different types of complicated pancreatic pseudocysts.Methods. The study included 47 patients aged 43.58±7.38 years: 42 males and 5 females. The patients were allocated to three groups based on the classification of pancreatic pseudocysts by A. D’Egidio and М. Schein (1991). All patients underwent surgery. Serum levels of vascular endothelial growth factor, matrix metalloproteinase-9, matrix metalloproteinase-2 tissue inhibitor, the complex of metalloproteinase-9 and inhibitor, plasma levels of interleukin-6, -8, -18 and -10 were determined by ELISA method. Blood glutathione peroxidase activity and malondialdehyde serum level were determined by spectrophotometry.Results.Levels of Interleukin-6, -8 and -18 was higher in patients with type I of pseudocysts compared to control (pConclusion. Increased concentration of matrix metalloproteinase-2 tissue inhibitor and increased inhibition coefficient in patients with chronic pancreatitis may reflect favorable conditions for pancreatic remodeling and fibrosis.

Sign in / Sign up

Export Citation Format

Share Document