Alteration of Neutrophil Reactive Oxygen Species Production by Extracts of Devil’s Claw (Harpagophytum)

Harpagophytum, Devil’s Claw, is a genus of tuberiferous xerophytic plants native to southern Africa. Some of the taxa are appreciated for their medicinal effects and have been traditionally used to relieve symptoms of inflammation. The objectives of this pilot study were to investigate the antioxidant capacity and the content of total phenols, verbascoside, isoverbascoside, and selected iridoids, as well as to investigate the capacity of variousHarpagophytumtaxa in suppressing respiratory burst in terms of reactive oxygen species produced by human neutrophils challenged with phorbol myristate acetate (PMA), opsonisedStaphylococcus aureus,andFusobacterium nucleatum.Harpagophytumplants were classified into different taxa according to morphology, and DNA analysis was used to confirm the classification. A putative new variety ofH. procumbensshowed the highest degree of antioxidative capacity. Using PMA, threeHarpagophytumtaxa showed anti-inflammatory effects with regard to the PBS control. A putative hybrid betweenH. procumbensandH. zeyheriin contrast showed proinflammatory effect on the response of neutrophils toF. nucleatumin comparison with treatment with vehicle control.Harpagophytumtaxa were biochemically very variable and the response in suppressing respiratory burst differed. Further studies with larger number of subjects are needed to corroborate anti-inflammatory effects of different taxa ofHarpagophytum.

Download Full-text

Anti-inflammatory effects of Erythromycin and Tetracycline on Propionibacterium. acnes induced production of chemotactic factors and reactive oxygen species by human neutrophils

Dermatology Online Journal ◽

10.5070/d304975508 ◽

2002 ◽

Vol 8 (2) ◽

Author(s):

A Jain ◽

L Sangal ◽

E Basal ◽

P Kaushal G ◽

SK Agarwal

Keyword(s):

Reactive Oxygen Species ◽

Propionibacterium Acnes ◽

Reactive Oxygen ◽

Human Neutrophils ◽

Oxygen Species ◽

Chemotactic Factors ◽

Anti Inflammatory

Download Full-text

Helicobacter pylori water extracts prime human neutrophils for enhanced reactive oxygen species production and stimulate production of CXC chemokine but not CC chemokine

Gastroenterology ◽

10.1016/s0016-5085(08)83250-7 ◽

2001 ◽

Vol 120 (5) ◽

pp. A654

Author(s):

Tadashi Shimoyama ◽

Qiang Liu ◽

Shinsaku Fukuda ◽

Takashi Umeda ◽

Ichiro Mizuki ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Helicobacter Pylori ◽

Reactive Oxygen Species Production ◽

Reactive Oxygen ◽

Human Neutrophils ◽

Oxygen Species ◽

Water Extracts ◽

Cc Chemokine ◽

Cxc Chemokine ◽

Species Production

Download Full-text

Simultaneous use of electrochemistry and chemiluminescence to detect reactive oxygen species produced by human neutrophils

Cell Biology International ◽

10.1016/j.cellbi.2008.08.016 ◽

2008 ◽

Vol 32 (12) ◽

pp. 1486-1496 ◽

Cited By ~ 12

Author(s):

Sergey Shleev ◽

Jonas Wetterö ◽

Karl-Eric Magnusson ◽

Tautgirdas Ruzgas

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Human Neutrophils ◽

Oxygen Species ◽

Simultaneous Use

Download Full-text

Inflammation and Anti-Inflammatory Agents – Reactive Oxygen Species and Toxicity

Systems Biology of Free Radicals and Antioxidants ◽

10.1007/978-3-642-30018-9_147 ◽

2014 ◽

pp. 3197-3216 ◽

Cited By ~ 1

Author(s):

Peter Kovacic ◽

Ratnasamy Somanathan

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Oxygen Species ◽

Anti Inflammatory

Download Full-text

The Effect of Physical Training on Peripheral Blood Mononuclear Cell Ex Vivo Proliferation, Differentiation, Activity, and Reactive Oxygen Species Production in Racehorses

Antioxidants ◽

10.3390/antiox9111155 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1155

Author(s):

Olga Witkowska-Piłaszewicz ◽

Rafał Pingwara ◽

Anna Winnicka

Keyword(s):

Reactive Oxygen Species ◽

Cell Proliferation ◽

Immune Cell ◽

Ex Vivo ◽

Exercise Physiology ◽

Reactive Oxygen ◽

Oxygen Species ◽

Peripheral Blood Mononuclear ◽

Before And After ◽

Anti Inflammatory

Physical activity has an influence on a variety of processes in an athlete’s organism including the immune system. Unfortunately, there is a lack of studies regarding racehorse immune cells, especially when the horse model is compared to human exercise physiology. The aim of the study was to determine changes in immune cell proliferation, lymphocyte populations, and monocyte functionality in trained and untrained racehorses after exercise. In this study, field data were collected. The cells from 28 racehorses (14 untrained and 14 well-trained) were collected before and after exercise (800 m at a speed of about 800 m/min) and cultured for 4 days. The expression of CD4, CD8, FoxP3, CD14, MHCII, and CD5 in PBMC, and reactive oxygen species (ROS) production, as well as cell proliferation, were evaluated by flow cytometry. In addition, IL-1β, IL-4, IL-6, IL-10, IL-17, INF-γ, and TNF-α concentrations were evaluated by ELISA. The creation of an anti-inflammatory environment in well-trained horses was confirmed. In contrast, a pro-inflammatory reaction occurred in untrained horses after training. In conclusion, an anti-inflammatory state occurs in well-trained racehorses, which is an adaptational reaction to an increased workload during training.

Download Full-text

The Arabidopsis RboHB Encoded by At1g09090 Is Important for Resistance against Nematodes

International Journal of Molecular Sciences ◽

10.3390/ijms21155556 ◽

2020 ◽

Vol 21 (15) ◽

pp. 5556 ◽

Cited By ~ 1

Author(s):

Abdalmenem I. M. Hawamda ◽

Adil Zahoor ◽

Amjad Abbas ◽

Muhammad Amjad Ali ◽

Holger Bohlmann

Keyword(s):

Reactive Oxygen Species ◽

Respiratory Burst ◽

Reactive Oxygen ◽

Heterodera Schachtii ◽

Oxygen Species ◽

Cyst Nematodes ◽

Feeding Site ◽

Plant Life ◽

Respiratory Burst Oxidase ◽

Respiratory Burst Oxidase Homologue

Reactive oxygen species are a byproduct of aerobic metabolic processes but are also produced by plants in defense against pathogens. In addition, they can function as signaling molecules that control various aspects of plant life, ranging from developmental processes to responses to abiotic and biotic stimuli. In plants, reactive oxygen species can be produced by respiratory burst oxidase homologues. Arabidopsis contains 10 genes for respiratory burst oxidase homologues that are involved in different aspects of plant life. Plant pathogenic cyst nematodes such as Heterodera schachtii induce a syncytium in the roots of host plants that becomes a feeding site which supplies nutrients throughout the life of the nematode. In line with this function, the transcriptome of the syncytium shows drastic changes. One of the genes that is most strongly downregulated in syncytia codes for respiratory burst oxidase homologue B. This gene is root-specific and we confirm here the downregulation in nematode feeding sites with a promoter::GUS (β-glucuronidase) line. Overexpression of this gene resulted in enhanced resistance against nematodes but also against leaf-infecting pathogens. Thus, respiratory burst oxidase homologue B has a role in resistance. The function of this gene is in contrast to respiratory burst oxidase homologues D and F, which have been found to be needed for full susceptibility of Arabidopsis to H. schachtii. However, our bioinformatic analysis did not find differences between these proteins that could account for the opposed function in the interaction with nematodes.

Download Full-text

Involvement of Caspases in Neutrophil Apoptosis: Regulation by Reactive Oxygen Species

Blood ◽

10.1182/blood.v92.12.4808 ◽

1998 ◽

Vol 92 (12) ◽

pp. 4808-4818 ◽

Cited By ~ 236

Author(s):

Bengt Fadeel ◽

Anders Åhlin ◽

Jan-Inge Henter ◽

Sten Orrenius ◽

Mark B. Hampton

Keyword(s):

Reactive Oxygen Species ◽

Cell Death ◽

Nadph Oxidase ◽

Reactive Oxygen ◽

Human Neutrophils ◽

Neutrophil Apoptosis ◽

Dependent Manner ◽

Caspase Activity ◽

Oxygen Species ◽

Spontaneous Apoptosis

Abstract Human neutrophils have a short half-life and are believed to die by apoptosis or programmed cell death both in vivo and in vitro. We found that caspases are activated in a time-dependent manner in neutrophils undergoing spontaneous apoptosis, concomitant with other characteristic features of apoptotic cell death such as morphologic changes, phosphatidylserine (PS) exposure, and DNA fragmentation. The treatment of neutrophils with agonistic anti-Fas monoclonal antibodies (MoAbs) significantly accelerated this process. However, in cells treated with the potent neutrophil activator phorbol 12-myristate 13-acetate (PMA), caspase activity was only evident after pharmacologic inhibition of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. Similarily, inhibition of the NADPH oxidase in constitutive and Fas/APO-1–triggered apoptosis resulted in increased rather than suppressed levels of caspase activity, suggesting that reactive oxygen species may prevent caspases from functioning optimally in these cells. Moreover, oxidants generated via the NADPH oxidase were essential for PS exposure during PMA-induced cell death, but not for neutrophils undergoing spontaneous apoptosis. We conclude that caspases are an important component of constitutive and Fas/APO-1–triggered neutrophil apoptosis. However, these redox sensitive enzymes are suppressed in activated neutrophils, and an alternate oxidant-dependent pathway is used to mediate PS exposure and neutrophil clearance under these conditions.

Download Full-text