Conservation of the Nrf2-Mediated Gene Regulation of Proteasome Subunits and Glucose Metabolism in Zebrafish

The Keap1-Nrf2 system is an evolutionarily conserved defense mechanism against oxidative and xenobiotic stress. Besides the exogenous stress response, Nrf2 has been found to regulate numerous cellular functions, including protein turnover and glucose metabolism; however, the evolutionary origins of these functions remain unknown. In the present study, we searched for novel target genes associated with the zebrafish Nrf2 to answer this question. A microarray analysis of zebrafish embryos that overexpressed Nrf2 revealed that 115 candidate genes were targets of Nrf2, including genes encoding proteasome subunits and enzymes involved in glucose metabolism. A real-time quantitative PCR suggested that the expression of 3 proteasome subunits (psma3, psma5, and psmb7) and 2 enzymes involved in glucose metabolism (pgd and fbp1a) were regulated by zebrafish Nrf2. We thus next examined the upregulation of these genes by an Nrf2 activator, diethyl maleate, using Nrf2 mutant zebrafish larvae. The results of real-time quantitative PCR and whole-mount in situ hybridization showed that all of these 5 genes were upregulated by diethyl maleate treatment in an Nrf2-dependent manner, especially in the liver. These findings implied that the Nrf2-mediated regulation of the proteasome subunits and glucose metabolism is evolutionarily conserved among vertebrates.

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Accurate, Fast and Cost-Effective Diagnostic Test for Monosomy 1p36 Using Real-Time Quantitative PCR

Disease Markers ◽

10.1155/2014/836082 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8

Author(s):

Pricila da Silva Cunha ◽

Heloisa B. Pena ◽

Carla Sustek D’Angelo ◽

Celia P. Koiffmann ◽

Jill A. Rosenfeld ◽

...

Keyword(s):

Real Time ◽

Diagnostic Test ◽

Quantitative Pcr ◽

Target Genes ◽

Cost Effective ◽

Qpcr Assay ◽

Deletion Syndrome ◽

Comparative Genomic ◽

Real Time Quantitative Pcr ◽

Subtelomeric Deletion

Monosomy 1p36 is considered the most common subtelomeric deletion syndrome in humans and it accounts for 0.5–0.7% of all the cases of idiopathic intellectual disability. The molecular diagnosis is often made by microarray-based comparative genomic hybridization (aCGH), which has the drawback of being a high-cost technique. However, patients with classic monosomy 1p36 share some typical clinical characteristics that, together with its common prevalence, justify the development of a less expensive, targeted diagnostic method. In this study, we developed a simple, rapid, and inexpensive real-time quantitative PCR (qPCR) assay for targeted diagnosis of monosomy 1p36, easily accessible for low-budget laboratories in developing countries. For this, we have chosen two target genes which are deleted in the majority of patients with monosomy 1p36:PRKCZandSKI. In total, 39 patients previously diagnosed with monosomy 1p36 by aCGH, fluorescentin situhybridization (FISH), and/or multiplex ligation-dependent probe amplification (MLPA) all tested positive on our qPCR assay. By simultaneously using these two genes we have been able to detect 1p36 deletions with 100% sensitivity and 100% specificity. We conclude that qPCR ofPRKCZandSKIis a fast and accurate diagnostic test for monosomy 1p36, costing less than 10 US dollars in reagent costs.

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Gene expression profile analysis of primary glioblastomas and non-neoplastic brain tissue: identification of potential target genes by oligonucleotide microarray and real-time quantitative PCR

Journal of Neuro-Oncology ◽

10.1007/s11060-008-9579-4 ◽

2008 ◽

Vol 88 (3) ◽

pp. 281-291 ◽

Cited By ~ 70

Author(s):

Carlos A. Scrideli ◽

Carlos G. Carlotti ◽

Oswaldo K. Okamoto ◽

Vanessa S. Andrade ◽

Maria A. A. Cortez ◽

...

Keyword(s):

Gene Expression ◽

Real Time ◽

Brain Tissue ◽

Quantitative Pcr ◽

Gene Expression Profile ◽

Target Genes ◽

Profile Analysis ◽

Real Time Quantitative Pcr ◽

Tissue Identification ◽

Expression Profile Analysis

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Profiling the lncRNA-miRNA-mRNA Interaction Network in the Submandibular Gland of Diabetic Mice

10.21203/rs.3.rs-677075/v1 ◽

2021 ◽

Author(s):

Xijin Shi ◽

Huimin Liu ◽

Li Li ◽

Yan Zhang ◽

Xin Cong ◽

...

Keyword(s):

Real Time ◽

Submandibular Gland ◽

Quantitative Pcr ◽

Target Genes ◽

Interaction Network ◽

Differentially Expressed ◽

Bioinformatics Analyses ◽

Real Time Quantitative Pcr ◽

Network Analyses ◽

Cerna Network

Abstract Background: Hyposalivation is one of the common symptoms of diabetes. Although long non-coding RNAs (lncRNAs) have recently been reported to play important roles in the pathogenesis of diabetes, the role of lncRNAs in diabetes-induced hyposalivation remains unknown. Methods: The present study aimed to explore the function of lncRNA-microRNA-mRNA regulatory network in the submandibular gland (SMGs) under the context of diabetes. LncRNA expression profile of the SMGs was analyzed using microarray technology. Differentially expressed lncRNAs were confirmed using real-time quantitative PCR. Bioinformatics analyses were performed, and Coding-non-coding gene co-expression (CNC) and competing endogenous RNA (ceRNA) networks were constructed to explore the potential mechanisms of diabetes-induced hyposalivation. Results: A total of 1,273 differentially expressed lncRNAs (536 up-regulated and 737 downregulated) were identified in the SMGs tissues of db/db mice. CNC and ceRNA network analyses were performed based on five differentially expressed lncRNAs validated by real-time quantitative PCR. Gene Ontology analysis of target genes of CNC network revealed that “calcium ion binding” was a highly enriched molecular function. Kyoto Encyclopedia of Genes and Genomes pathway analysis of target genes of ceRNA network revealed that the “mammalian target of rapamycin signaling pathway” was significantly enriched. Conclusions: On the whole, the findings of the present study may provide insight into the possible mechanism of diabetes-induced hyposalivation.

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Selection of stable reference genes for real-time quantitative PCR in honey bee pesticide toxicity studies

Journal of Apicultural Research ◽

10.1080/00218839.2021.1950972 ◽

2021 ◽

pp. 1-11

Author(s):

Sanghyeon Kim ◽

Susie Cho ◽

Si Hyeock Lee

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Honey Bee ◽

Reference Genes ◽

Pesticide Toxicity ◽

Real Time Quantitative Pcr ◽

Toxicity Studies ◽

Selection Of

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A papaya-specific gene, papain, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic papayas

European Food Research and Technology ◽

10.1007/s00217-008-0935-6 ◽

2008 ◽

Vol 228 (2) ◽

pp. 301-309 ◽

Cited By ~ 15

Author(s):

Wentao Xu ◽

Weibin Bai ◽

Feng Guo ◽

Yunbo Luo ◽

Yanfang Yuan ◽

...

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Reference Gene ◽

Pcr Detection ◽

Specific Gene ◽

Real Time Quantitative Pcr ◽

Endogenous Reference Gene ◽

Endogenous Reference

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Development of a rapid detection and quantification method of Karenia mikimotoi by real-time quantitative PCR

Harmful Algae ◽

10.1016/j.hal.2012.03.004 ◽

2012 ◽

Vol 17 ◽

pp. 83-91 ◽

Cited By ~ 41

Author(s):

Jian Yuan ◽

Tiezhu Mi ◽

Yu Zhen ◽

Zhigang Yu

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Rapid Detection ◽

Karenia Mikimotoi ◽

Real Time Quantitative Pcr ◽

Quantification Method ◽

Detection And Quantification

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Use of real-time quantitative PCR to investigate root and gall colonisation by co-inoculated isolates of the nematophagous fungusPochonia chlamydosporia

Annals of Applied Biology ◽

10.1111/j.1744-7348.2009.00333.x ◽

2009 ◽

Vol 155 (1) ◽

pp. 143-152 ◽

Cited By ~ 20

Author(s):

S.D. Atkins ◽

B. Peteira ◽

I.M. Clark ◽

B.R. Kerry ◽

P.R. Hirsch

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Real Time Quantitative Pcr

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Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

Applied and Environmental Microbiology ◽

10.1128/aem.00965-06 ◽

2006 ◽

Vol 72 (12) ◽

pp. 7894-7896 ◽

Cited By ~ 193

Author(s):

Silvia Bofill-Mas ◽

Nestor Albinana-Gimenez ◽

Pilar Clemente-Casares ◽

Ayalkibet Hundesa ◽

Jesus Rodriguez-Manzano ◽

...

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

High Stability ◽

Wastewater Sludge ◽

Human Polyomavirus ◽

Sewage Effluent ◽

Viral Contamination ◽

Human Adenoviruses ◽

Urban Sewage ◽

Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

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