scholarly journals Microbiological Quality Assessment of Frozen Fish and Fish Processing Materials from Bangladesh

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Sohana Al Sanjee ◽  
Md. Ekramul Karim
Keyword(s):  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Plate Count ◽  
Most Probable Number ◽  
Human Consumption ◽  
Microbiological Analysis ◽  
Fish Processing ◽  
Fish Pathogens ◽  
Probable Number ◽  
Total Coliforms

The present study aims at the microbiological analysis of export oriented frozen fishes, namely, Jew fish, Tongue Sole fish, Cuttle fish, Ribbon fish, Queen fish, and fish processing water and ice from a view of public health safety and international trade. Microbiological analysis includes the determination of total viable aerobic count by standard plate count method and enumeration of total coliforms and fecal coliforms by most probable number method. The presence of specific fish pathogens such asSalmonellaspp. andVibrio choleraewere also investigated. The TVAC of all the samples was estimated below5×105 cfu/g whereas the total coliforms and fecal coliforms count were found below 100 MPN/g and 10 MPN/g, respectively, which meet the acceptable limit specified by International Commission of Microbiological Specification for Food. The microbiological analysis of water and ice also complies with the specifications havingTVAC<20 cfu/mL, and total coliforms and fecal coliforms count were below the limit detection of the MPN method. Specific fish pathogens such asSalmonellasp. andV. choleraewere found absent in all the samples under the investigation. From this study, it can be concluded that the investigated frozen fishes were eligible for export purpose and also safe for human consumption.

scholarly journals MICROBIOLOGICAL EVALUATION OF HYDRIC RESOURCES PROVIDED BY THE TIETÊ RIVER, DISTRICT OF VITORIANA, BOTUCATU, SP, BRAZIL

2006 ◽  
Vol 73 (3) ◽  
pp. 283-286
Author(s):  
T.A.F. Corrêa ◽  
V.L.M. Rall ◽  
M.G. Silva ◽  
C.A.M. Lopes
Keyword(s):  
Infectious Diseases ◽  
Water Samples ◽  
Sao Paulo ◽  
Fecal Coliforms ◽  
Most Probable Number ◽  
Human Consumption ◽  
Probable Number ◽  
Total Coliforms ◽  
Tietê River ◽  
Recreational Use

ABSTRACT Water, fundamental to the existence of mankind, demands the attention of competent authorities since its contamination could become a vehicle for transmission of infectious diseases. Thus the present work aimed to investigate the presence of fecal and total coliforms in water samples taken from wells, a lake and both the margin and channel of the Tiete River, Barra Bonita Dam, Botucatu, São Paulo, Brazil. The necessity of investigation arose by virtue of a high gastrenteritis index detected in the population that utilizes these hydric resources. The most probable number (MPN) of fecal and total coliforms was determined for 168 water samples through the method of multiple tubes. The results showed that only 42% of the samples collected in 4 wells investigated were considered of good quality for human consumption, by not presenting fecal coliforms in 100 mL of water, according to Decree Nº 518 de 25/103/2004. Regarding suitability for bathing/recreational use, according to CONAMA Resolution 357 of 17/03/2005, it was observed that in the locations investigated, only 10% of the samples analyzed were considered inappropriate for bathing and 90% of the remainder were considered satisfactory.

Comparison of culturable fecal coliforms and Escherichia coli enumeration in freshwaters

2007 ◽  
Vol 53 (6) ◽  
pp. 798-801 ◽  
Author(s):  
Tamara Garcia-Armisen ◽  
Josué Prats ◽  
Pierre Servais
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Quality Data ◽  
Plate Count ◽  
Most Probable Number ◽  
Tetrazolium Chloride ◽  
Probable Number ◽  
E Coli ◽  
Glucuronidase Activity

Fecal coliforms (FC) counts were compared with Escherichia coli counts in differently contaminated freshwater samples (n = 166). FC were enumerated by plate count on triphenyl 2,3,5-tetrazolium chloride Tergitol medium. Escherichia coli were enumerated by the most probable number microplate method based on the detection of glucuronidase activity. FC and E. coli counts were highly correlated; an average E. coli/FC ratio equal to 0.77 was found, meaning that on average, 77% of FC were E. coli. Knowing the E. coli/FC ratio allows us to convert the historical microbiological quality data expressed in FC counts into E. coli abundance and thus to compare with present and future monitoring data that are (or will be) based on E. coli enumeration.

Microbiological Quality and Incidence of Salmonella on Cherry Tomatoes at Retail in Querétaro, México

2018 ◽  
Vol 81 (4) ◽  
pp. 614-618 ◽  
Author(s):  
MARLA G. LEAL-CERVANTES ◽  
SOFÍA M. ARVIZU-MEDRANO ◽  
RAMÓN MARTÍNEZ-PENICHE ◽  
NANCI E. MARTÍNEZ-GONZÁLES ◽  
MONTSERRAT HERNÁNDEZ-ITURRIAGA
Keyword(s):  
Sampling Site ◽  
Microbiological Quality ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
Local Markets ◽  
Total Coliforms ◽  
E Coli ◽  
Aerobic Plate Count ◽  
Cherry Tomatoes

ABSTRACT Multiple outbreaks related to Salmonella in tomatoes require an evaluation of the risk associated with cherry tomatoes due to the increase in its production, consumption, and marketing in Mexico's central region. The purpose of this study was to determine the microbial quality of cherry tomatoes obtained from two retail sale points (supermarkets and local markets). Cherry tomato samples (333) were collected from four supermarkets and from four local markets, and the contents of aerobic plate count, molds and yeasts, total coliforms, and Escherichia coli were quantified; the presence of Salmonella was simultaneously determined. The median values of the microbial populations were obtained, and the data were analyzed per the sampling site by using the Wilcoxon and Kruskal-Wallis tests. The median of aerobic plate count content in tomatoes obtained from supermarkets ranged between 2.2 and 4.4 log CFU/g, and in markets from 2.9 to 4.8 log CFU/g. For molds and yeasts, the tomatoes from supermarkets (2.0 to 4.1 log CFU/g) and markets (1.5 to 4.5 log CFU/g) showed similar contents. Regardless of the sampling site, the values of total coliforms were very low, ranging from 1.0 to 1.8 log CFU/g. E. coli was detected in 5.4 and 20.1% of samples from supermarkets and markets, respectively; in both sites, the content was low (0.3 to 5.8 most probable number per g). The incidence of Salmonella was 14.1% in supermarkets and 7.8% in local markets. The results obtained from this investigation highlight the elevated risk for consumer health associated with the ingestion of cherry tomatoes.

scholarly journals AVALIAÇÃO DA QUALIDADE MICROBIOLÓGICA DO GENGIBRE IN NATURA COMERCIALIZADO NA REGIÃO METROPOLITANA DE CURITIBA, PR

2004 ◽  
Vol 5 (2) ◽  
Author(s):  
Eliane Rose Serpe ELPO ◽  
Raquel Rejane Bonato NEGRELLE ◽  
Eliane Carneiro GOMES
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
The Other ◽  
Most Probable Number ◽  
Microbiological Analysis ◽  
Probable Number ◽  
Total Coliforms ◽  
Other Hand ◽  
Post Harvesting

Analisou-se 10 amostras de gengibre “in natura” com objetivo de caracterizar o perfil microbiológico deste produto comercializado na região metropolitana de Curitiba, PR (Brasil). Para tanto, foram realizadas a determinação do número mais provável (NMP) de coliformes totais, coliformes a 45º C/g e Escherichia coli, e a presença de Salmonella sp em 25 gramas. Os resultados indicaram que 100% das amostras atenderam ao padrão da legislação vigente para Salmonella sp, enquanto que 30% das amostras de gengibre enquadradas como raízes, tubérculos e similares, e cerca de 50% das amostras como especiarias, temperos, condimentos e similares apresentaram coliformes a 45º C/g. Destas amostras analisadas, 20% confirmaram a presença de Escherichia coli. Observou-se que todas as amostras mostraram elevados índices de coliformes totais, apesar de não haver limite máximo estabelecido pela legislação vigente. Os resultados obtidos podem ser indicativos de inadequações nos procedimentos de beneficiamento pós-colheita e de comercialização. Abstract Ten samples of natural ginger, commercialized in Curitiba, Paraná (Brazil), were analyzed for microbiological quality. The microbiological analysis measured the total coliforms, 45° C/g coliforms, the most probable number (MPN) of Escherichia coli and the presence of Salmonella sp. The results revealed that 100% of the samples complied with the required standard for the absence of Salmonella sp. On the other hand, 30% - 50% had 45º C/g coliforms and 20% of them contained Escherichia coli. It was observed that all the samples had a high value for the total coliforms. This is important since the current legislation does not establish any limits. The results obtained may indicate inadequate procedures for post-harvesting and commercial handling.

scholarly journals Microbiological quality study of Apis mellifera honey produced in the Cerrado - MT

2010 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Dayane Aparecida dos Santos ◽  
Angela Kwiatkowski ◽  
Marcos Vieira da Silva ◽  
Dalany Menezes ◽  
Lucimar Peres de Moura Pontara
Keyword(s):  
Ph Value ◽  
Microbiological Quality ◽  
Soluble Solids ◽  
Most Probable Number ◽  
Microbiological Analysis ◽  
Mato Grosso ◽  
Probable Number ◽  
Physical And Chemical ◽  
The Town

<p>Monitoring the microbiology of honey is necessary to ensure its final quality. The microbial contamination is a danger to public health, a major concern for the surveillance agency. The objective of this study was to evaluate the microbiological quality of honey produced in the cerrado region, Mato Grosso State. The apiary was installed in the town of Conquista D'Oeste city, a total of 30 boxes of honey covered with asbestos tile and exposed to the sun. The honey was collected in November of 2008. The physical and chemical analysis were carried out: moisture, total soluble solids (TSS) and pH. Microbiological analysis were: mesophilic bacteria facultative aerobic, yeast and mold, Staphylococcus, the most probable number (MPN) of coliforms at 35&ordm;C and 45&ordm;C, and the presence of Salmonella sp. The methods used followed the directions of the Normative Instruction no. 62, August 23, 2003 of Minist&eacute;rio da Agricultura, Pecu&aacute;ria e Abastecimento - MAPA. The pH value resulted in 3.80, SST at 81.5 and humidity at 17 %. Microbiological analysis showed no presence of Salmonella, Staphylococcus and yeast. The count of facultative aerobic bacteria was 5.0 x 101, the two groups of coliforms in NMP was &lt;3.0, and 3.0 x 101 mold. The samples of honey studied showed appropriate microbiological quality.</p><p>&nbsp;</p><p><span>DOI:&nbsp;</span><a href="http://dx.doi.org/10.14685/rebrapa.v4i1.102"><span>http://dx.doi.org/10.14685/rebrapa.v1i1.5</span></a><span>&nbsp;</span></p><p>&nbsp;</p>

scholarly journals Sensory Evaluation of grape yogurt with added calcium and zinc

2010 ◽  
Vol 1 (2) ◽  
pp. 125
Author(s):  
Ivani De Carvalho ◽  
Marinês De Souza ◽  
Ornella Maria Porcu
Keyword(s):  
Purchase Intention ◽  
Aerobic Bacteria ◽  
Most Probable Number ◽  
Human Consumption ◽  
Microbiological Analysis ◽  
High Solids ◽  
Probable Number ◽  
Intent To Purchase ◽  
Hedonic Scale ◽  
Yeasts And Molds

<p>Yogurt is a product widely recommended due to its sensory, nutritional and probiotic characteristics. It is made of milk containing high solids, lactic acid culture and sugar and can also be enriched. The objectives were to develop a grape yogurt with added calcium and zinc, and evaluate its microbiological and sensory characteristics and verify the consumer&rsquo;s acceptance and intent to purchase this product. A formulation of yoghurt enriched with calcium and zinc was elaborated. The microbiological analysis (most probable number (MPN) of coliforms at 35&nbsp;&ordm;C and 45&nbsp;&ordm;C (thermotolerant) Total count of mesophilic aerobic bacteria, <em>Staphylococcus</em> spp., yeasts and molds) indicated that the product was in agreement with existing national legislation for the identity and quality standards. The overall acceptance was conducted with 100 untrained panelists using a 9-point Hedonic Scale (1 - dislike extremely, 9 - like extremely), averaging more than 70.00 % to the questions of taste, flavor, color, sweetness, consistency and overall assessment. As to purchase intention, it was found that 77.00 % of consumers would buy grape yogurt with added calcium and zinc indicating that the formulation is feasible and acceptable for human consumption.</p><p>&nbsp;</p><p>DOI: http://dx.doi.org/10.14685/rebrapa.v1i2.28</p>

A Microbial Survey of Various Fresh and Frozen Seafood Products

1977 ◽  
Vol 40 (5) ◽  
pp. 300-303 ◽  
Author(s):  
JAMES F. FOSTER ◽  
JAMES L. FOWLER ◽  
JOHN DACEY
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Microbiological Quality ◽  
Retail Store ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
Seafood Products ◽  
Aerobic Plate Count ◽  
Fresh Products

The microbiological quality of four frozen and seven fresh seafood products (597 units in total) obtained from a local retail store were analyzed. Aerobic plate count means (geometric) ranged from 3.5 × 103/g to 9.3 × 104/g for the frozen products and from 7.8 × 104/g to 2.7 × 108/g for fresh products. Average (geometric) coliform Most Probable Number (MPN) values ranged from 1.0 to 7. 7/g for the frozen items and from 7.8/g to 4.8 × 103/g for the fresh seafoods. Employing the MPN method, 4.7% of the 597 units analyzed were positive for Escherichia coli. while 7.9% were positive for Staphylococcus aureus. Two percent of the samples contained Clostridium perfringens. Neither salmonellae nor Vibrio parahaemolyticus was isolated in any of the 597 units.

Comparison of a Rapid Plate Count and MPN Methods for Enumeration of Fecal Coliforms and Escherichia coli in Soft-Shell Clams

1995 ◽  
Vol 58 (11) ◽  
pp. 1197-1200 ◽  
Author(s):  
GUADALUPE R. GARCIA ◽  
ROBERT E. HAYMOND ◽  
DIANE M. SPRAGUE ◽  
EMMA R. SINGLETON ◽  
JAMES T. PEELER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fecal Coliform ◽  
Mya Arenaria ◽  
Fecal Coliforms ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
Rapid Procedure ◽  
Soft Shell ◽  
Plate Count Method

A direct elevated temperature plate count method utilizing modified fecal coliform agar with rosolic acid (ETPC/mFC) was compared to 5-tube and 3-tube most probable number (MPN) procedures for its accuracy in enumerating fecal coliforms and Escherichia coli in naturally and artificially contaminated soft-shell clams (Mya arenaria). The results indicated that the extent of overall recovery of fecal coliforms was similar in the two methods tested. Therefore, the ETPC/mFC method may be considered as a rapid procedure for fecal coliform screening during depuration of soft-shell clams.

scholarly journals MICROBIOLOGICAL QUALITY OF GOAT MILK PRODUCED ON ALAGOAS STATE, BRAZIL

2019 ◽  
Vol 11 (2) ◽  
pp. 47-56
Author(s):  
Erica Guedes Celestino ◽  
Micheline Thaís Santos ◽  
Sybelle Georgia Silva ◽  
Tania Marta Carvalho dos Santos ◽  
Elizabeth Simões do Amaral Alves ◽  
...  
Keyword(s):  
Microbiological Quality ◽  
Goat Milk ◽  
Raw Milk ◽  
Most Probable Number ◽  
Probable Number ◽  
Pasteurized Milk ◽  
Total Coliforms ◽  
Specific Medium

The objective of this study was to verify the microbiological quality of raw and pasteurized goat milk and produced in Alagoas state, Brazil. Was carried out three interval collect of pasteurized and raw milk and the samples taken to the laboratory. All samples were subjected to aerobic mesophilic microorganisms counting, psychrotrophic microorganisms, determination of the most probable number (MPN) of total coliforms and thermotolerant microorganisms. For analysis, samples were plated on specific medium (Baird-Parker Agar Base himedia M043) and incubated at 37 °C for 24h. To Salmonella sp. detection was used plating the medium Salmonella Shigella agar. The presence of coliforms at 35 °C was detected in all samples as well as 45 °C except to first sample. To the pasteurized milk was found to coliforms at 35 °C and 45 °C in the second and third samples. Only in the second test was checked the presence of mesofilic bacteria. We have not found samples contaminated with microorganisms psychrotrophic, Salmonella sp. and Staphylococcus sp. According to the obtained results the quality of collected milk has unacceptable conditions of consumption compared to coliform counts, according to the parameters established by law.

scholarly journals Bacteriological Quality of Drinking Water in Mardan, Khyber Pakhtunkhwa, Pakistan

2015 ◽  
Vol 21 ◽  
pp. 1-6
Author(s):  
Javid Ali ◽  
Said Hassan ◽  
Dr Ziaurahman ◽  
Inayat Ur Rahman ◽  
Sadhair Abbas ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Fecal Coliforms ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
Khyber Pakhtunkhwa ◽  
E Coli ◽  
Micro Organisms

The present study was aimed to isolate and identify micro-organisms load of drinking water of Mardan city, Khyber Pakhtunkhwa Pakistan. A total of 27 samples of drinking water were collected from different locations of the study area. Total Plate Count was determined by pour plate method, while total coliforms, total fecal coliforms and E. coli were determined by multiple tube fermentation method. Of the total collected samples, 17 (62.96%) samples were contaminated with either one or more than one type of microorganisms. The results of most probable number test showed that 13 (48.15%) samples were unsatisfactory. It was concluded that the water should be treated before consumption for drinking purpose. Regular assessment of the water quality is recommended as regular monitoring of the water quality for improvement not only prevents disease and hazards but also checks the water resources from becoming further polluted. ECOPRINT 21: 1-6, 2014DOI: http://dx.doi.org/10.3126/eco.v21i0.11897

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