scholarly journals Deregulation of Regulatory T Cells in Acute-on-Chronic Liver Failure: A Rat Model

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Shunlan Ni ◽  
Shanshan Li ◽  
Naibin Yang ◽  
Xinyue Tang ◽  
Shengguo Zhang ◽  
...  
Keyword(s):  
Liver Failure ◽  
Rat Model ◽  
Th17 Cell ◽  
Interleukin 10 ◽  
Treg Cells ◽  
Chronic Liver ◽  
Control Group ◽  
Chronic Liver Failure ◽  
T Helper 17 ◽  
Cell Proportion

Aims. Acute-on-chronic liver failure (ACLF) and acute liver failure (ALF) are similar in many respects during their acute exacerbation; however, ACLF generally has a poorer prognosis. We aimed to investigate the role and dynamic changes of regulatory T cell (Treg) and T helper 17 (Th17) cell proportions during ACLF progress. Methods. All rats were classified into two groups randomly: ACLF group and ALF group (control group). The rat model of ACLF was preestablished by intraperitoneal injection of carbon tetrachloride for 2 months. Then acute liver injury was induced by combined D-galactosamine and lipopolysaccharide. Six time points were examined before or after acute induction. Liver samples were performed with hematoxylin-eosin and Masson staining; circulatory Treg and Th17 cell frequencies were determined using flow cytometry assays; serum levels of alanine aminotransferase, aspartate aminotransferase, interleukin-10 (IL-10), and interferon-γ (IFN-γ) were examined. Results. In group ACLF, both Th17 cell proportion and IFN-γ level presented upgrade firstly and then descend latter tendency; the trends of Treg cell proportion and IL-10 level were observed to gradually decrease and became stable. Conclusion. The Treg cells played an important role in the immunologic mechanism during the process of ACLF. And the function of Treg cells in ACLF was defective.

scholarly journals The effect of fractal-like mechanical ventilation on vital signs in a rat model of acute-on-chronic liver failure

2018 ◽  
Vol 39 (11) ◽  
pp. 114008
Author(s):  
Arman Nataj ◽  
Golnar Eftekhari ◽  
Mohammad R Raoufy ◽  
Ali R Mani
Keyword(s):  
Mechanical Ventilation ◽  
Liver Failure ◽  
Rat Model ◽  
Vital Signs ◽  
Chronic Liver ◽  
Chronic Liver Failure

Regulation of IL-22 in a rat model for acute-on-chronic liver failure (ACLF)

2020 ◽  
Vol 73 ◽  
pp. S498
Author(s):  
Katharina Maria Schwarzkopf ◽  
Frank Uschner ◽  
Robert Schierwagen ◽  
Sabine Klein ◽  
Marcus Maximilian Muecke ◽  
...  
Keyword(s):  
Liver Failure ◽  
Rat Model ◽  
Chronic Liver ◽  
Chronic Liver Failure

scholarly journals Lower level of complement component C3 and C3a in the plasma means poor outcome in the patients with hepatitis B virus related acute-on-chronic liver failure

2020 ◽  
Author(s):  
Qian Li ◽  
Qing Lu ◽  
Meng-Qi Zhu ◽  
Chong Huang ◽  
Kang-Kang Yu ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Liver Failure ◽  
Plasma Concentrations ◽  
Chronic Liver ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Chronic Liver Failure ◽  
Complement Components ◽  
B Virus

Abstract Background The purpose of this study was to investigate whether or not the complement system is activated systemically activated and to specify the clinical and prognostic implications of its components during hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF).Methods Blood samples from twenty-seven patients diagnosed with HBV-ACLF, twenty-five patients diagnosed with chronic hepatitis B but without liver failure, and nine health volunteers were enrolled in this survey. Plasma complement components were measured with Enzyme-linked immunosorbent assay. Correlative analysis were assessed between the levels of complement components and the liver failure related index.Results The concentrations of C1q, C3, C3a, C4, C4a and sC5b-9 were significantly higher in the control group than those in the HBV-ACLF group (3.5, 2.4, 2.1, 1.4, 1.3 and 6.0 fold, respectively). However, there was no statistical significance of the differences in the plasma concentrations of mannose binding lectin and factor B between the HBV-ACLF and control groups. The levels of C3 and C3a were inversely correlated with MELDs (P < 0.05) or CLIF-C OFs (P < 0.05).Conclusions Our data demonstrated that the plasma levels of C3 and C3a might be potential novel biomarkers to predict the outcome of HBV-ACLF.

Cirrhosis Is a Predictor of Adverse Events in Endoscopic Ultrasound Fine-Needle Aspiration: A Propensity-Score Analysis

2019 ◽  
Vol 38 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Antonio Facciorusso ◽  
Vincenzo Rosario Buccino ◽  
Valentina Del Prete ◽  
Matteo Antonino ◽  
Nicola Muscatiello
Keyword(s):  
Propensity Score ◽  
Adverse Events ◽  
Liver Failure ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Chronic Liver ◽  
Control Group ◽  
Chronic Liver Failure ◽  
Fine Needle ◽  
Cirrhotic Patients

Background: Cirrhotic patients are at increased risk of adverse events (AEs) after invasive procedures. However, the safety profile of endoscopic ultrasound (EUS) fine-needle aspiration in cirrhotic patients is still unknown. Objectives: To examine the AEs rate after EUS fine-needle aspiration in cirrhotic patients as compared to a control group of noncirrhotic patients. Methods: Out of 735 patients with suspected abdominal lesions referred to our center between 2006 and 2018, after propensity-score matching, 2 groups were compared: 95 cirrhotics and 95 controls. Primary variables included rate of overall and serious AEs. Secondary endpoint was incidence of acute-on-chronic liver failure. Results: Median age was 64 years, and pancreatic lesions represented the sampled tissue in 57 patients (60%) in each group (p = 1.0) with mean size of 22 mm in both cohorts (p = 0.3). Overall, 20 AEs (21%) of which 9 serious (9.4%) were observed in cirrhotic patients and 3 (3.1%) mild events were experienced by noncirrhotic subjects. Only liver cirrhosis was confirmed as a significant predictor of AEs in multivariate analysis (OR 8.11, 2.34–28; p = 0.001). Overall rate of infections was 6/95 (6.3%), of which 4 were serious (4.2%) in cirrhotics and 1/95 (1%) in the control group (p = 0.05). All serious infections led to the occurrence of acute-on-chronic liver failure. Minor bleeding events were more frequent in cirrhotic patients (4.2 vs. 0%; p = 0.04). Conclusions: The rate of AEs after EUS fine-needle aspiration is higher in cirrhotic patients, and acute-on-chronic liver failure may represent a serious complication of this procedure.

scholarly journals Jieduan-Niwan Formula Reduces Liver Apoptosis in a Rat Model of Acute-on-Chronic Liver Failure by Regulating the E2F1-Mediated Intrinsic Apoptosis Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Wenlong Yang ◽  
Yulin Hao ◽  
Weixin Hou ◽  
Xian Fang ◽  
Peng Fang ◽  
...  
Keyword(s):  
Liver Failure ◽  
Rat Model ◽  
Pathological Condition ◽  
Survival Rates ◽  
Chronic Liver ◽  
Group Model ◽  
Chronic Liver Failure ◽  
Apoptotic Pathway ◽  
Apoptosis Pathway ◽  
Intrinsic Apoptosis Pathway

Acute-on-chronic liver failure (ACLF) is a serious and complicated disease that threatens human health because its pathogenesis is unclear, and the outcome of the current therapies has been less than satisfactory. A national famous doctor of traditional Chinese medicine, Qian Ying, created the Jieduan-Niwan Formula (JDNW), based on his long-term clinical experience. However, despite the good clinical outcome, the biological mechanism by which it works is unknown. In the current study, we established an ACLF rat model by administering human serum albumin (HSA) combined with D-galactosamine (D-GalN) and lipopolysaccharide (LPS) to explore the potential mechanism of JDNW in treating ACLF. The rats were treated with JDNW by administration of the model substances and sacrificed after 4, 8, and 12 h. Then we divided the rats into normal group, model at 4 h, model at 8 h, model at 12 h, JDNW at 4 h, JDNW at 8 h, and JDNW at 12 h. Biochemical and histopathological examinations were performed to compare the rats in different groups. Compared with the ACLF model group, expression levels of alanine transaminase, aspartate aminotransferase, total bilirubin, and TNF-α and IL-6 proteins were reduced in the JDNW group at the corresponding time points, the survival rates of rats were increased, and the pathological condition of the liver was improved. In addition, JDNW treatment improved the ultrastructure of hepatocytes and mitochondria and decreased the hepatocyte apoptosis index. E2F1, P53, P73, Apaf-1, p14ARF, caspase-3, caspase-6, and caspase-7 levels in the JDNW group were distinctly lower than those in the untreated rats. Moreover, Bcl-2 and Mcl-1 levels increased. Thus, JDNW decreases ACLF-induced mortality in rats by modulating the E2F1-mediated intrinsic apoptotic pathway.

scholarly journals The Effect of Modified Sini Decoction on Survival Rates of Patients with Hepatitis B Virus Related Acute-on-Chronic Liver Failure

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Jian-Xing Luo ◽  
Yang Zhang ◽  
Xiao-Yu Hu ◽  
Chang Yu
Keyword(s):  
Internal Medicine ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Liver Failure ◽  
Survival Rates ◽  
Chronic Liver ◽  
Control Group ◽  
Observation Group ◽  
Chronic Liver Failure ◽  
B Virus

Aim of the Study.To verify the effect of modified sini decoction on patients with hepatitis B virus related acute-on-chronic liver failure.Materials and Methods.A retrospective cohort study was conducted. Patients who had been treated with modified sini decoction and standard comprehensive internal medicine were assigned to an observation group, and patients who had been treated with standard comprehensive internal medicine were selected as a control group. The total bilirubin (TBIL), albumin (ALB), alanine aminotransferase (ALT), prothrombin activity (PTA), CTP, and MELD scores were analyzed at weeks 4, 8, and 12 after treatment, respectively. Meanwhile, the 12-week survival rate was analyzed.Results.The levels of TBIL and ALT were remarkably decreased, while the levels of ALB and PTA were remarkably increased in both groups at weeks 4, 8, and 12 after treatment, respectively, but the effects in the observation group were greater (P < 0.05). The CTP and MELD scores at 8-week and 12-week were lower in the observation group than in the control group (P < 0.05). At 12 weeks, the mean survival times of the observation group and the control group were 66.7 and 45.5 d, respectively. Significant improvement of 12-week survival rate [39/62 (62.9%) versus 18/50 (36.0%), P = 0.001] was observed in the observation group after treatment.Conclusions.Modified sini decoction could protect the liver function and improve the survival rates of patients with hepatitis B virus related acute-on-chronic liver failure.

scholarly journals Retrospective Analysis of the Clinical Efficacy of N-Acetylcysteine in the Treatment of Hepatitis B Virus Related Acute-on-Chronic Liver Failure

2021 ◽  
Vol 8 ◽  
Author(s):  
Meng-Lan Wang ◽  
Xiu-Jun Yin ◽  
Xue-Lian Li ◽  
Fa-Da Wang ◽  
Jing Zhou ◽  
...  
Keyword(s):  
Treatment Group ◽  
Liver Failure ◽  
Intrahepatic Cholestasis ◽  
Chronic Liver ◽  
Control Group ◽  
Chronic Liver Failure ◽  
Baseline Serum ◽  
Treatment Measures ◽  
New Treatment ◽  
Coagulation Dysfunction

Objective: HBV-related acute-on-chronic liver failure (HBV-ACLF) has a high mortality due to severe intrahepatic cholestasis and coagulation dysfunction, thus new treatment measures are urgently needed to improve the therapeutic effect. This study aimed to observe the efficacy of N-acetylcysteine (NAC) in the treatment of HBV-ACLF.Methods: The data of patients with HBV-ACLF admitted to West China Hospital from October 2019 to August 2020 were collected retrospectively, and they were divided into treatment group and control group according to whether they had received additional NAC treatment. The improvement of biochemistry, coagulation function and disease severity score after 14 days of hospitalization were analyzed between two groups.Results: A total of 90 HBV-ACLF patients were included, including 42 patients in treatment group and 48 patients in control group. Compared with baseline, serum TBil, DBil, TBA, GGT and ALP in two groups both decreased significantly, while PTA increased significantly. Interesting, the decrease of serum TBil, DBil and TBA and the increase of PTA in treatment group were all significantly than these in control group. Additionally, more patients in treatment group than control group changed from CTP grade C to grade B. Subgroup analysis of CTP grade C patients showed that the decrease of serum TBil, DBil and TBA and the increase of PTA in treatment group were significantly than these in control group.Conclusion: The NAC treatment may help to improve intrahepatic cholestasis and coagulation dysfunction of HBV-ACLF.

scholarly journals Plasma Interleukin-10: A Likely Predictive Marker for Hepatitis B Virus-Related Acute-on-Chronic Liver Failure

2014 ◽  
Vol 14 (7) ◽  
Author(s):  
Ke Wang ◽  
Zhe-bin Wu ◽  
Yi-nong Ye ◽  
Jing Liu ◽  
Geng-lin Zhang ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Liver Failure ◽  
Predictive Marker ◽  
Interleukin 10 ◽  
Chronic Liver ◽  
Chronic Liver Failure ◽  
B Virus
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