scholarly journals Infections Caused by HRSV A ON1 Are Predominant among Hospitalized Infants with Bronchiolitis in São Paulo City

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Sandra E. Vieira ◽  
Luciano M. Thomazelli ◽  
Milena de Paulis ◽  
Angela E. Ferronato ◽  
Daniele B. Oliveira ◽  
...  
Keyword(s):  
Respiratory Infections ◽  
Phylogenetic Analyses ◽  
Length Of Hospital Stay ◽  
Sao Paulo ◽  
High Rate ◽  
São Paulo ◽  
Positive Rate ◽  
Nasopharyngeal Secretion ◽  
The Mean ◽  
Syncytial Virus

Human respiratory syncytial virus is the main cause of respiratory infections in infants. Several HRSV genotypes have been described. Goals. To describe the main genotypes that caused infections in São Paulo (2013–2015) and to analyze their clinical/epidemiological features. Methods. 94 infants (0–6 months) with bronchiolitis were studied. Clinical/epidemiological information was collected; a search for 16 viruses in nasopharyngeal secretion (PCR-real-time and conventional, sequencing, and phylogenetic analyses) was performed. Results. The mean age was 2.4 m; 48% were male. The mean length of hospital stay was 4.4 d (14% in the Intensive Care Unit). The positive rate of respiratory virus was 98.9%; 73 cases (77.6%) were HRSV (76,7% HRSVA). HRSVA formed three clusters: ON1 (n=34), NA1 (n=1), and NA2 (n=4). All HRSVB were found to cluster in the BA genotype (BA9-n=10; BA10-n=3). Clinical analyses showed no significant differences between the genotype AON1 and other genotypes. Conclusion. This study showed a high rate of HRSV detection in bronchiolitis. HRSVA ON1, which has recently been described in other countries and has not been identified in previous studies in the southeast region of Brazil, was predominant. The clinical characteristics of the infants that were infected with AON1 were similar to infants with infections by other genotypes.

scholarly journals Respiratory syncytial virus B sequence analysis reveals a novel early genotype

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Juan C. Muñoz-Escalante ◽  
Andreu Comas-García ◽  
Sofía Bernal-Silva ◽  
Daniel E. Noyola
Keyword(s):  
Molecular Markers ◽  
Respiratory Syncytial Virus ◽  
Maximum Likelihood ◽  
Respiratory Infections ◽  
Phylogenetic Analyses ◽  
Detection Methods ◽  
Sequence Alignments ◽  
Multiple Sequence ◽  
Individual Gene ◽  
Syncytial Virus

AbstractRespiratory syncytial virus (RSV) is a major cause of respiratory infections and is classified in two main groups, RSV-A and RSV-B, with multiple genotypes within each of them. For RSV-B, more than 30 genotypes have been described, without consensus on their definition. The lack of genotype assignation criteria has a direct impact on viral evolution understanding, development of viral detection methods as well as vaccines design. Here we analyzed the totality of complete RSV-B G gene ectodomain sequences published in GenBank until September 2018 (n = 2190) including 478 complete genome sequences using maximum likelihood and Bayesian phylogenetic analyses, as well as intergenotypic and intragenotypic distance matrices, in order to generate a systematic genotype assignation. Individual RSV-B genes were also assessed using maximum likelihood phylogenetic analyses and multiple sequence alignments were used to identify molecular markers associated to specific genotypes. Analyses of the complete G gene ectodomain region, sequences clustering patterns, and the presence of molecular markers of each individual gene indicate that the 37 previously described genotypes can be classified into fifteen distinct genotypes: BA, BA-C, BA-CC, CB1-THB, GB1-GB4, GB6, JAB1-NZB2, SAB1, SAB2, SAB4, URU2 and a novel early circulating genotype characterized in the present study and designated GB0.

Seroepidemiological study of respiratory syncytial virus in São Paulo State, Brazil

1998 ◽  
Vol 55 (3) ◽  
pp. 234-239 ◽  
Author(s):  
M. J. Cox ◽  
R. S. Azevedo ◽  
P. A. Cane ◽  
E. Massad ◽  
G. F. Medley
Keyword(s):  
Respiratory Syncytial Virus ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State ◽  
Syncytial Virus

scholarly journals Gastrointestinal nematode infections in sheep raised in Botucatu, state of São Paulo, Brazil

2014 ◽  
Vol 23 (3) ◽  
pp. 348-354 ◽  
Author(s):  
Maurício Orlando Wilmsen ◽  
Bruna Fernanda Silva ◽  
César Cristiano Bassetto ◽  
Alessandro Francisco Talamini do Amarante
Keyword(s):  
Haemonchus Contortus ◽  
Natural Infection ◽  
Gastrointestinal Nematode ◽  
Gastrointestinal Nematodes ◽  
Sao Paulo ◽  
São Paulo ◽  
Mean Intensity ◽  
Oesophagostomum Columbianum ◽  
The Mean ◽  
In Parenthesis

Gastrointestinal nematode infections were evaluated in sheep raised in Botucatu, state of São Paulo, Brazil between April 2008 and March 2011. Every month, two tracer lambs grazing with a flock of sheep were exposed to natural infection with gastrointestinal nematodes for 28 consecutive days. At the end of this period, the lambs were sacrificed for worm counts. Haemonchus contortus presented 100% of prevalence. The seasons exerted no significant influence on the mean intensity of H. contortus, which ranged from 315 worms in November 2010 to 2,5205 worms in January 2011. The prevalence of Trichostrongylus colubriformis was also 100%, with the lowest mean intensity (15 worms) recorded in February 2011 and the highest (9,760 worms) in October 2009. In the case of T. colubriformis, a significant correlation coefficient was found between worm counts vs. rainfall (r = −0.32; P <0.05). Three other nematodes species were found in tracer lambs, albeit in small numbers. Their prevalence and mean intensity (in parenthesis) were as follows: Oesophagostomum columbianum 28% (25.2), Cooperia curticei 7% (4.5) and Trichuris spp. 2% (1). In conclusion, the environmental conditions of the area proved to be highly favorable for the year-round transmission of H. contortus and T. colubriformis.

Clathrosporium retortum, a novel aeroaquatic fungus in the Sordariomycetidae (Ascomycota) from Brazil

Phytotaxa ◽  
2015 ◽  
Vol 239 (1) ◽  
pp. 17
Author(s):  
Larissa Bernardino Moro ◽  
Gregorio Delgado ◽  
Iracema Helena SCHOENLEIN-CRUSIUS
Keyword(s):  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Type Specimen ◽  
Molecular Data ◽  
Sao Paulo ◽  
São Paulo ◽  
Present Fungus ◽  
Broad Concept ◽  
Morphological And Molecular Data ◽  
First Time

Clathrosporium retortum sp. nov., collected on submerged mixed leaf litter samples at Ilha do Cardoso State Park, São Paulo state, Brazil, is described based on morphological and molecular data. The fungus is characterized by forming whitish, dense, subglobose to irregular propagules, hyaline to subhyaline when young, subhyaline to dark brown at maturity, that are formed by densely interwoven conidial filaments with each conidial cell repeatedly branching bilaterally or occasionally unilaterally. Phylogenetic analyses using partial LSU nrDNA sequence data suggest that C. retortum belongs in the Sordariomycetes (Ascomycota) where it forms a well-supported clade with Clohesia corticola in the Sordariomycetidae, but its ordinal or familial placement remains unresolved. Its phylogenetic placement confirms the polyphyletic nature of aeroaquatic fungi like Clathrosporium, as it was distantly related to one available sequence in GenBank named as C. intricatum, the type species, which is phylogenetically related to the Helotiales (Leotiomycetes). However, due to lack of authenticity of the identity of this sequence with the type specimen of C. intricatum, a broad concept of Clathrosporium is tentatively adopted here to accommodate the present fungus instead of introducing a new genus. Beverwykella clathrata, Helicoön septatissimum and Peyronelina glomerulata are recorded for the first time from Brazil. Cancellidium applanatum and Candelabrum brocchiatum are new records for the state of São Paulo.

scholarly journals Surveillance of influenza A H1N1 2009 among school children during 2009 and 2010 in São Paulo, Brazil

2012 ◽  
Vol 45 (5) ◽  
pp. 563-566 ◽  
Author(s):  
Sandra Baltazar Guatura ◽  
Aripuana Sakurada Aranha Watanabe ◽  
Clarice Neves Camargo ◽  
Ana Maria Passos ◽  
Sheila Negrini Parmezan ◽  
...  
Keyword(s):  
School Children ◽  
Influenza A ◽  
Respiratory Infections ◽  
Vaccination Campaign ◽  
Sao Paulo ◽  
Morbidity Rate ◽  
High Morbidity ◽  
São Paulo ◽  
High Morbidity Rate ◽  
Influenza A H1n1

INTRODUCTION: Influenza A H1N1 2009 is associated with a high morbidity rate among children around the world, including Brazil. This survey was conducted on samples of symptomatic children (< 12 years) to investigate the influenza virus as the etiological agent of respiratory infections in a day care school in a health facility during the first and second pandemic wave of H1N1 (2009-2010) in São Paulo, Brazil. METHODS: Influenza infections were determined by real-time PCR in 34% (47/137) of children with a median age of 5 years (8 months - 12 years), from June to October 2009 and in 16% (14/85) of those with median age of 6 years (1-12 years), from March to November 2010. RESULTS: In general, most positive cases (64%) occurred in children aged 5-12 years, this age group was significantly the most affected (39.8%, p = 0.001, OR = 8.3, CI 95% 1.9-36.9). Wheezing was reported by 31% (19/61) and dyspnea by 23% (14/61) of the studied patients. An outbreak of influenza H1N1 with an attack rate of 35.7% among children (median age 6 years) was documented in April 2010, before the vaccination campaign against the pandemic virus was extended for children up to 5 years in Brazil. CONCLUSIONS: Therefore, the study reinforces the recommendation to immunize school children to reduce the incidence of the disease.

scholarly journals Protozooplankton and its relationship with environmental conditions in 13 water bodies of the Mogi-Guaçu basin - SP, Brazil

2013 ◽  
Vol 13 (4) ◽  
pp. 152-163 ◽  
Author(s):  
Inessa Lacativa Bagatini ◽  
Ana Lúcia Gerardi Spínola ◽  
Bianca de Miranda Peres ◽  
Adrislaine da Silva Mansano ◽  
Mafalda Alexandra Antunes Rodrigues ◽  
...  
Keyword(s):  
Environmental Variables ◽  
Sao Paulo ◽  
Water Bodies ◽  
Bromophenol Blue ◽  
São Paulo ◽  
Paulo State ◽  
Two Samples ◽  
São Paulo State ◽  
Biological Aspects ◽  
The Mean

Protozooplankton is an important component of the aquatic microbial food webs and its composition, density, and distribution reflect the chemical, physical, and biological aspects of the environment. Considering the scarce literature on freshwater protozoans in Brazil and on protozoan ecology in subtropical environments, we listed the ciliates and amoebae taxa found in 13 water bodies in São Paulo State and analyzed their abundance in relation to the environmental variables. We collected two samples in each environment, fixed immediately with mercuric chloride and stained with bromophenol blue. After microscopical analysis, 74 protozoan genera were identified and the Ciliophora were dominant in the majority of the environments. The Stichotrichia, represented mostly by the genus Halteria, occurred in all environments, and was the dominant subclass in five of them. The canonic correspondence analysis of the most frequent genera and the environmental variables showed that nitrite and nitrate were the variables that better explained the distribution of Limnostrombidium, Urotricha, and Vorticella. The densities of the genera Halteria, Coleps, and of the species Cinetochilum margaritaceum were positively affected by increasing concentrations of dissolved oxygen, particulate phosphate, conductivity, and temperature. C. margaritaceum were also negatively affected by increasing concentrations of nitrite and nitrate. Considering that we made only one sampling in each environment, the richness was high compared to the mean diversity of lakes in the São Paulo State. The Diogo Lake, located in an ecological reserve, was the richest one, confirming the need of more research on the biodiversity of more preserved environments.

Geographical clusters and social risk factors for suicide in the city of São Paulo, 2006–2015: An ecologic study

2020 ◽  
Vol 66 (5) ◽  
pp. 460-468
Author(s):  
Daniel Hideki Bando ◽  
Ligia Vizeu Barrozo ◽  
Fernando Madalena Volpe
Keyword(s):  
Risk Factors ◽  
Suicide Rate ◽  
Religious Affiliation ◽  
Sao Paulo ◽  
São Paulo ◽  
Multiple Model ◽  
Increased Risk ◽  
The Mean ◽  
Risk Factors For Suicide ◽  
Geographical Clusters

Background: To identify geographical clusters of suicide in São Paulo, Brazil (2006–2015) and to verify the associations of suicide with sociocultural characteristics of its 96 districts. Methods: Spatial scan test was used to detect the geographical clusters. Correlation and multiple regression techniques were used to estimate the association of socioeconomic and cultural variables with suicide. Results: The mean suicide rate was 4.8/100,000. Three clusters were identified which are as follows: one of increased risk in downtown and two of decreased risk in the South and in the Southeast. The mean suicide rate of the high-risk clustered districts (7.99/100,000) presented significantly higher average incomes per household, higher proportion of formally educated, of no religious affiliation, of recent migrants, of all-times migrants and lower proportion of married. The multiple model selected two independent risk factors – people with no religious affiliation (β = 0.182) and of recent migrants (β = 0.278) – and two protective factors – the proportion of married (β = –0.185) and of total migrants (β = –0.075), which jointly explained 58.4% of the variance. Conclusion: Durkheimian social and cultural risk factors for suicide were confirmed. Compared to a previous study period (1996–2005), suicide rates and geographical clusters remained relatively stable in the subsequent decade (2006–2015).

scholarly journals Canine rabies epidemiology in Araçatuba and neighborhood, Northwestern São Paulo State - Brazil

2004 ◽  
Vol 37 (2) ◽  
pp. 139-142 ◽  
Author(s):  
Luzia Helena Queiroz da Silva ◽  
César Eduardo Bissoto ◽  
Ádna Cleia B. Delbem ◽  
Clara Isabel de Lucca Ferrari ◽  
Sílvia Helena Venturoli Perri ◽  
...  
Keyword(s):  
Urban Areas ◽  
Sao Paulo ◽  
São Paulo ◽  
Canine Rabies ◽  
Paulo State ◽  
São Paulo State ◽  
The Mean ◽  
Northwest Region ◽  
Epidemiological Characteristics ◽  
Rabies Diagnosis

Epidemiological characteristics of canine rabies in the northwest region of São Paulo State (Araçatuba region), Brazil, from 1993 to 1997 are presented. Out of 1,984 dogs, a total of 351 (17.7%) were positive for rabies diagnosis; 89% (312/351) of these occurred in urban areas and 85% (266/312) of the urban positive cases were among owned dogs. The mean age of the rabid dogs was 34 months and 61% were male. Aggressive behavior was observed in 77% of rabid dogs, followed by lack of coordination and paralysis (42%) and 48% of these dogs were responsible biting people or other animals. Information about vaccination status was obtained from 182 records and 51% of rabid dogs were non-vaccinated. The number of unvaccinated rabid dogs indicates a low vaccination index and this factor added to the high dog/man ratio must have contributed to the canine rabies epizootic observed in the studied area.

scholarly journals Behaviors and hygiene habits of complete denture wearers

2010 ◽  
Vol 21 (3) ◽  
pp. 247-252 ◽  
Author(s):  
Amanda Peracini ◽  
Ingrid Machado de Andrade ◽  
Helena de Freitas Oliveira Paranhos ◽  
Cláudia Helena Lovato da Silva ◽  
Raphael Freitas de Souza
Keyword(s):  
Oral Care ◽  
Sao Paulo ◽  
Complete Denture ◽  
Dental School ◽  
Complete Dentures ◽  
São Paulo ◽  
Mechanical Method ◽  
Limited Knowledge ◽  
Cleaning Method ◽  
The Mean

In this study, a questionnaire was applied to patients from Ribeirão Preto Dental School, University of São Paulo, Brazil, to evaluate the hygiene methods and habits concerning the use of complete dentures, the age of dentures, and whether patients have been instructed on how to clean their dentures. The mean age of patients was 63.35 years, and most of them were females (82.08%). The results showed that 62.26% of the respondents had been using the same maxillary complete denture for more than 5 years, and 49.06% used the same mandible complete denture for more than 5 years. Of the patients interviewed, 58.49% slept with the dentures. Mechanical brushing was the most used cleaning method by the patients (100%), using water, dentifrice and toothbrush (84.91%). Most patients (51.89%) reported never having been instructed by their dentists as to how to clean their dentures. Based on the limitations of this study, it was concluded that the patients interviewed had limited knowledge about prosthetic hygiene and oral care. The method more used by patients was the mechanical method of brushing, most patients used the same complete dentures for more than 5 years and slept with the dentures.

scholarly journals Cognitive function and risk factors in the older population: differences between the sexes

2021 ◽  
Author(s):  
Vinícius Lúcio de Barros ◽  
Larissa Gomes Peres Bomfim ◽  
Melyssa Alves Souza ◽  
Danielle Ginsicke ◽  
Mileny Esbravatti Stephano Colovati ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Mini Mental State Examination ◽  
Mmse Score ◽  
The Elderly ◽  
Sao Paulo ◽  
São Paulo ◽  
Brain Health ◽  
The Mean ◽  
State Examination ◽  
Study Inclusion

Background: Studies have shown a sex difference in the cognitive dysfunction in the elderly where women are more susceptible to Alzheimer’s disease than men. Physical activity (PA) is an important strategy for brain health, but the exact mechanism is unclear. Santos has the largest proportion of the elderly population in São Paulo State (22%), but no studies have whether there are differences between the sexes in cognitive function. Objective: To evaluate the differences between the sexes in the cognitive function of the elderly and which factors are associated with this difference. Methods: Ninety-seven women and sixty-nine men were eligible for the study. Inclusion criteria were >65 years old, no severe cognitive impairment, preserved activities of daily living. Cognitive function and PA were assessed by the mini-mental state examination (MMSE) and Baecke questionnaire. Results: The mean age was 75±7 and 73±7, for women and men, respectively. Both sexes showed a similar MMSE score (26, varying between 14 and 30) that was inversely associated with age. The influence of the schooling status on MMSE was greater in women (r=0.49, p=0.0001) compared to men (r= 0.30, p9. Conclusions: The MMSE score is affected by the aging process similarly between the sexes. The years of schooling have an influence on cognitive function in women that is attenuated by higher PA. Ethical Committee: CAAE 20938619.4.0000.5509. Financial Support: São Paulo Research Foundation.

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