scholarly journals Cytoprotective Mechanisms Mediated by Polyphenols from Chilean Native Berries against Free Radical-Induced Damage on AGS Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Felipe Ávila ◽  
Cristina Theoduloz ◽  
Camilo López-Alarcón ◽  
Eva Dorta ◽  
Guillermo Schmeda-Hirschmann
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenger ◽  
Glyoxalase I ◽  
Protective Effects ◽  
Glutathione S Transferase ◽  
Ags Cells ◽  
Cellular Protection ◽  
Glutathione S Transferases

The prevalence of cytoprotective mechanisms induced by polyphenols such as activation of intracellular antioxidant responses (ICM) and direct free radical scavenging was investigated in native Chilean species of strawberries, raspberries, and currants. Human gastric epithelial cells were co- and preincubated with polyphenolic-enriched extracts (PEEs) from Chilean raspberries (Rubus geoides), strawberries (Fragaria chiloensis ssp. chiloensis f. chiloensis), and currants (Ribes magellanicum) and challenged with peroxyl and hydroxyl radicals. Cellular protection was determined in terms of cell viability, glyoxalase I and glutathione s-transferases activities, and carboxymethyl lysine (CML) and malondialdehyde levels. Our results indicate that cytoprotection induced by ICM was the prevalent mechanism for Rubus geoides and F. chiloensis. This agreed with increased levels of glyoxalase I and glutathione S-transferase activities in cells preincubated with PEEs. ORAC index indicated that F. chiloensis was the most efficient peroxyl radical scavenger. Moreover, ICM mediated by F. chiloensis was effective in protecting cells from CML accumulation in contrast to the protective effects induced by free radical scavenging. Our results indicate that although both polyphenol-mediated mechanisms can exert protective effects, ICM was the most prevalent in AGS cells. These results suggest a potential use of these native berries as functional food.

scholarly journals Bergenin Content and Free Radical Scavenging Activity of Bergenia Extracts

2015 ◽  
Vol 10 (7) ◽  
pp. 1934578X1501000
Author(s):  
Helena Hendrychová ◽  
Jan Martin ◽  
Lenka Tůmová ◽  
Nina Kočevar-Glavač
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hplc Method ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Free Radical Scavengers ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Different Seasons

Our research was focused on the evaluation of bergenin content and free radical scavenging activity of extracts prepared from three different species of Bergenia - B. crassifolia (L.) Fritsch., B. ciliata (Haw.) Sternb. and B. x ornata Stein. collected during different seasons. Using an HPLC method, the highest total amount of bergenin was revealed in the leaves of B. x ornata and B. crassifolia (4.9 - 5.1 mg.g−1). Free radical scavenging power was determined by two methods - FRAP and NADH. The best free radical scavengers were B. crassifolia (FRAP: 6.7 - 15.9 mg GAE.100g−1; NADH: 20.3 - 50.9%) and B. ornata (FRAP: 13.7 - 15.2 mg GAE.100g−1; NADH: 29.3 - 31.1%). The lowest content of bergenin and the weakest radical scavenger was B. ciliata (bergenin: 3.1 mg.g−1; FRAP: 5.5 - 11.0 mg GAE.100g−1; NADH: 23.2 - 25.6%). The presence of a large percentage of bergenin is responsible for the radical scavenging activity, as shown by the results from the FRAP and NADH assays. Significant, positive correlation was found between bergenin content and radical scavenging activity in both methods.

scholarly journals Extraction Optimization of Galla Turcica Polysaccharides and Determination of Its Antioxidant Activities In Vitro

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091176
Author(s):  
Jiangxia Hu ◽  
Jiayu Gao ◽  
Zijun Zhao ◽  
Xiao Yang ◽  
Lan Chen
Keyword(s):  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Protective Effects ◽  
Ultrasonic Time ◽  
Health Products ◽  
Total Antioxidant ◽  
Ultrasonic Assisted

Though natural polysaccharides commonly show antioxidant activities, the current research on the isolation of polysaccharides from Galla Turcica and their antioxidant activities still remain as an ongoing challenge. In this work, response surface analysis was employed to optimize an ultrasonic-assisted extraction method for polysaccharides of Galla Turcica. Their antioxidant and free radical scavenging activities were then evaluated using 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), total antioxidant activity, and iron ion reduction assays. Moreover, the protective effects of polysaccharides of Galla Turcica were determined on human embryonic kidney fibroblast 293 and hepatoma 7721 cells by cell proliferation assay. Overall, the key parameters of Galla Turcica polysaccharides extraction were optimized as crushing degree 100 mesh, ultrasonic time 50 min, and materials–liquid ratio 1:50. The isolated polysaccharides presented dose-dependent antioxidant and free radical scavenging effects in vitro. It also demonstrated an effective protective effect for human cells under oxidative damage. The results firstly determined the antioxidant activities of polysaccharides from Galla Turcica, thus providing a new natural resource for future investigation and development of the polysaccharides-based antioxidant drugs, health products, or additives.

Antioxidant, free radical scavenging and liver protective effects of friedelin isolated from Azima tetracantha Lam. leaves

2013 ◽  
Vol 139 (1-4) ◽  
pp. 860-865 ◽  
Author(s):  
Christudas Sunil ◽  
Veeramuthu Duraipandiyan ◽  
Savarimuthu Ignacimuthu ◽  
Naif Abdullah Al-Dhabi
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Protective Effects ◽  
Azima Tetracantha

Arjunic Acid, a Strong Free Radical Scavenger from Terminalia arjuna

2008 ◽  
Vol 36 (01) ◽  
pp. 197-207 ◽  
Author(s):  
Fang-Yun Sun ◽  
Xiu-Ping Chen ◽  
Jin-Hua Wang ◽  
Hai-Lin Qin ◽  
Su-Rong Yang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Dpph Method ◽  
Significant Difference

This study was designed to investigate the antioxidant and free radical scavenging capacities of arjunic acid, an aglycone obtained from the fruit of medicine Terminalia Fruit. Liver microsomes, mitochondria, and red blood cells (RBCs) were prepared from Wistar rats. The antioxidant capacity was determined by the inhibitory effect on lipid peroxidation, hydrogen peroxide induced RBCs hemolysis, and RBCs autoxidative hemolysis. The free radical scavenging activity was tested by DPPH method and 2′,7′-dichlorodihydrofluoresc in diacetate (DCFH2-DA) assay. Ascorbic acid was chosen as the positive controls. Results showed that arjunic acid was a strong antioxidant and a free radical scavenger, more potent than ascorbic acid, in microsomes lipid peroxidation, DPPH, hydrogen peroxide induced RBCs hemolysis, and (DCFH2-DA) assay (p < 0.05). However, no significant difference was observed in the RBCs autoxidative hemolysis assay (p > 0.05).

DPPH Free Radical Scavenger Components from the Fruits of Alpinia rafflesiana Wall. ex. Bak. (Zingiberaceae)

2004 ◽  
Vol 59 (11-12) ◽  
pp. 811-815 ◽  
Author(s):  
Habsah Mohamad ◽  
Faridah Abas ◽  
Dharma Permana ◽  
Nordin H. Lajis ◽  
Abdul Manaf Ali ◽  
...  
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Colorimetric Analysis ◽  
Natural Sources

The methanol extract of the dried ripe fruits of Alpinia rafflesiana was investigated for its DPPH free radical scavenger constituents. 2′,3′,4′,6′-Tetrahydroxychalcone (7), which has never been isolated from natural sources was found to be most active as a DPPH free radical scavenger with the IC50 value of 55 μᴍ. Other known compounds isolated from this species include 5,6-dehydrokawain (1), flavokawin B (2), 1,7-diphenyl-5-hydroxy-6-hepten-3-one (3), (-)-pinocembrin (4), cardamonin (5) and (-)-pinostrobin (6). The DPPH free radical scavenger compounds were detected using TLC autographic analysis. The percentage inhibition of DPPH free radical scavenging activity was measured on isolates (5-7) using colorimetric analysis.

scholarly journals Protective effects of olive oil phenolics oleuropein and hydroxytyrosol against hydrogen peroxide-induced DNA damage in human peripheral lymphocytes

2021 ◽  
Vol 71 (1) ◽  
pp. 131-141
Author(s):  
Nataša Zorić ◽  
Nevenka Kopjar ◽  
Jadranka Vuković Rodriguez ◽  
Siniša Tomić ◽  
Ivan Kosalec
Keyword(s):  
Dna Damage ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Olive Oil ◽  
Human Lymphocytes ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Protective Effects ◽  
Human Peripheral Lymphocytes ◽  
Primary Dna Damage

AbstractThis study investigates antioxidant capacity and protective effects of phenolic compounds oleuropein (OLP) and hydroxytyrosol (HT), present in olive oil and olive leaves, against H2O2-induced DNA damage in human peripheral lymphocytes. Antioxidant potency was determined using the measurement of radical-scavenging activity (ABTS∙+ assay), ferric reducing power (FRAP assay) and cupric reducing antioxidant capacity (CUPRAC assay). Both substances were found to be potent antioxidant agents due to their free radical-scavenging activities. Antigenotoxic effects of oleuropein and hydroxytyrosol against H2O2-induced damage in human lymphocytes were evaluated in vitro by alkaline comet assay. At tested concentrations (1, 5, 10 µmol L−1), oleuropein and hydroxytyrosol did not induce a significant increase of primary DNA damage in comparison with the negative control. Pretreatment of human lymphocytes with each of the substances for 120 min produced a dose-dependent reduction of primary DNA damage in the tested cell type. Hydroxytyrosol showed a better protective effect against H2O2-induced DNA breaks than oleuropein which could be associated with their free radical-scavenging efficacy.

scholarly journals Evaluation of free radical scavenging activity of leaf of Hypericum mysorense Heyne

2017 ◽  
Vol 9 (4) ◽  
pp. 589
Author(s):  
Sornalakshmi V ◽  
Tresina PS ◽  
Paulpriya K ◽  
Veerabahu Ramasamy Mohan
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Model Systems ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Reducing Ability ◽  
Scavenger Activity

<em>Hypericum </em>is a well-known plant genus in herbal medicine. <em>Hypericum mysorense</em> is well known in folklore medicine for its varied therapeutic potential. The <em>in vitro </em>antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extract of leaf of <em>Hypericum mysorense </em>have been tested using various antioxidant model systems viz; DPPH, hydroxyl, superoxide, ABTS and reducing power. The scavenging effect increases with the concentration of standard and samples. Among the solvent tested, ethanol extract exhibited highest DPPH (116.33% inhibition), hydroxyl (101.84%), superoxide (116.09%) and ABTS (119.22%) radical scavenger activity. Methanol extract of <em>H.mysorense </em>shows the highest reducing ability. The aim and purpose of this study is to show the significant free radical scavenging potential of <em>H.mysorense </em>leaf which can be experimented for the treatment of various free radical mediated ailments

Synthesis, antibacterial and free radical scavenging activity of some newer N-((10-nitro-1H-indolo [1, 2-c]quinazolin-12-yl)methylene)benzenamines

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
A. Dixit ◽  
D. Pathak ◽  
G.K. Sharma
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Diffusion Method ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Dpph Method ◽  
Antibacterial Potential

AbstractPresent research is oriented on the synthesis of some novel 12-(N-arylmethaniminyl)indolo[1,2-c]quinazoline analogs (4b1-4b11) and their characterization by 1H NMR, 13C NMR, FTIR and mass spectrophotometry. Their free radical scavenging activity and antibacterial potential were also evaluated. Many derivatives have shown a marked free radical scavenging capacity in all the concentrations but specifically compounds 4b7, 4b8 and 4b11 have shown good antioxidant potential with an IC50 value of 25.18 μmol/L, 28.09 μmol/L ---amp--- 44.22 μmol/L, respectively (DPPH method) and 39.46 μmol/L, 44.47 μmol/L ---amp--- 35.61 μmol/L, respectively (H2O2 method). The antibacterial evaluation was carried out against B. subtilis and E. coli by agar well diffusion method and it revealed that all the compounds in the series were having marked antibacterial activity but compounds 4b9 and 4b11 have shown best antibacterial potential. Then, it was concluded that the derivatives which were containing substituted anilines (4-Nitro, 4-Fluoro, 4-Bromo ---amp--- 4-Chloro-2-nitro) on the carbon attached on the 12th position of indoloquinazoline moiety were having marked potential as an antibacterial and free radical scavenger.

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