Arjunic Acid, a Strong Free Radical Scavenger from Terminalia arjuna

2008 ◽  
Vol 36 (01) ◽  
pp. 197-207 ◽  
Author(s):  
Fang-Yun Sun ◽  
Xiu-Ping Chen ◽  
Jin-Hua Wang ◽  
Hai-Lin Qin ◽  
Su-Rong Yang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Dpph Method ◽  
Significant Difference

This study was designed to investigate the antioxidant and free radical scavenging capacities of arjunic acid, an aglycone obtained from the fruit of medicine Terminalia Fruit. Liver microsomes, mitochondria, and red blood cells (RBCs) were prepared from Wistar rats. The antioxidant capacity was determined by the inhibitory effect on lipid peroxidation, hydrogen peroxide induced RBCs hemolysis, and RBCs autoxidative hemolysis. The free radical scavenging activity was tested by DPPH method and 2′,7′-dichlorodihydrofluoresc in diacetate (DCFH2-DA) assay. Ascorbic acid was chosen as the positive controls. Results showed that arjunic acid was a strong antioxidant and a free radical scavenger, more potent than ascorbic acid, in microsomes lipid peroxidation, DPPH, hydrogen peroxide induced RBCs hemolysis, and (DCFH2-DA) assay (p < 0.05). However, no significant difference was observed in the RBCs autoxidative hemolysis assay (p > 0.05).

DPPH Free Radical Scavenger Components from the Fruits of Alpinia rafflesiana Wall. ex. Bak. (Zingiberaceae)

2004 ◽  
Vol 59 (11-12) ◽  
pp. 811-815 ◽  
Author(s):  
Habsah Mohamad ◽  
Faridah Abas ◽  
Dharma Permana ◽  
Nordin H. Lajis ◽  
Abdul Manaf Ali ◽  
...  
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Colorimetric Analysis ◽  
Natural Sources

The methanol extract of the dried ripe fruits of Alpinia rafflesiana was investigated for its DPPH free radical scavenger constituents. 2′,3′,4′,6′-Tetrahydroxychalcone (7), which has never been isolated from natural sources was found to be most active as a DPPH free radical scavenger with the IC50 value of 55 μᴍ. Other known compounds isolated from this species include 5,6-dehydrokawain (1), flavokawin B (2), 1,7-diphenyl-5-hydroxy-6-hepten-3-one (3), (-)-pinocembrin (4), cardamonin (5) and (-)-pinostrobin (6). The DPPH free radical scavenger compounds were detected using TLC autographic analysis. The percentage inhibition of DPPH free radical scavenging activity was measured on isolates (5-7) using colorimetric analysis.

Synthesis, antibacterial and free radical scavenging activity of some newer N-((10-nitro-1H-indolo [1, 2-c]quinazolin-12-yl)methylene)benzenamines

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
A. Dixit ◽  
D. Pathak ◽  
G.K. Sharma
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Diffusion Method ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Dpph Method ◽  
Antibacterial Potential

AbstractPresent research is oriented on the synthesis of some novel 12-(N-arylmethaniminyl)indolo[1,2-c]quinazoline analogs (4b1-4b11) and their characterization by 1H NMR, 13C NMR, FTIR and mass spectrophotometry. Their free radical scavenging activity and antibacterial potential were also evaluated. Many derivatives have shown a marked free radical scavenging capacity in all the concentrations but specifically compounds 4b7, 4b8 and 4b11 have shown good antioxidant potential with an IC50 value of 25.18 μmol/L, 28.09 μmol/L ---amp--- 44.22 μmol/L, respectively (DPPH method) and 39.46 μmol/L, 44.47 μmol/L ---amp--- 35.61 μmol/L, respectively (H2O2 method). The antibacterial evaluation was carried out against B. subtilis and E. coli by agar well diffusion method and it revealed that all the compounds in the series were having marked antibacterial activity but compounds 4b9 and 4b11 have shown best antibacterial potential. Then, it was concluded that the derivatives which were containing substituted anilines (4-Nitro, 4-Fluoro, 4-Bromo ---amp--- 4-Chloro-2-nitro) on the carbon attached on the 12th position of indoloquinazoline moiety were having marked potential as an antibacterial and free radical scavenger.

scholarly journals Hydroxy-Tyrosol as a Free Radical Scavenging Molecule in Polymeric Hydrogels Subjected to Gamma-Ray Irradiation

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 433
Author(s):  
Mauro Fiorini ◽  
Veronica Crognaletti ◽  
Omar Sabry ◽  
Lorenzo Scalise ◽  
Paolo Fattori
Keyword(s):  
Free Radical ◽  
Gamma Ray ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenger ◽  
Cross Linking ◽  
Radical Scavenger ◽  
Gamma Ray Irradiation ◽  
Anti Oxidant ◽  
Polymeric Hydrogels

Biomedical engineering is employing hydrogels with increasingly exciting possibilities for the treatment and regeneration of pathologically altered, degenerated, or traumatized tissues. Still, the sterilization processes may undesirably change the chemical and physical properties of hydrogels through cross-linking reactions. This work aims to characterize a new method of producing polyethylene oxide (PEO) hydrogels exploiting hydroxy-tyrosol (HT), an anti-oxidant molecule derived from olive leaf and olive oil, as a free radical scavenger to either prevent or limit gamma-ray-induced cross-linking. For this purpose, we produced hydrogels with PEO with two different buffer solutions (phosphate and citrate), varying HT concentration. We analyzed hydrogel preparations before and after gamma-ray irradiation, assessing the viscosity through rheological analysis and the chemical changes through IR analysis. We performed high-performance liquid chromatography (HPLC) analysis to measure residual HT in hydrogels after irradiation. The obtained results show that radiation-induced cross-linking and increase in viscosity of PEO hydrogels can be prevented by tailoring the concentration of HT as a free radical scavenging agent. Irradiation only consumes small amounts of HT; its presence in polymeric hydrogels can significantly impact biomedical applications by its anti-oxidant and anti-microbial activities.

scholarly journals Investigation on antioxidants, free radical scavenger and lipid peroxidation activities of whole grains finger millet (Eleusine coracana L.)

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Abiodun Akeem Ajiboye ◽  
Oyinade Aderoju Dedeke ◽  
Folasade Comfort Adeyemo
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Finger Millet ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Whole Grain ◽  
Oxidation Stress ◽  
Radical Scavenging Ability

This study evaluates the antioxidant potential and free radical scavenging ability of whole grain finger millet. The crude extracts were obtained by cold maceration using 80% ethanol and methanol. Concentrated extracts were subjected to radicals of 2,2’-diphenyl-1-picrylhydrazyl hydrate (DPPH), hydrogen peroxide, nitric oxide (NO) and malondialdehyde, while the antioxidant constituents evaluated includes total phenol and flavonoid. The results revealed that whole grain finger millet contained 14.65±0.41 and 38.19±0.60 mg QUE/g sample of flavoniods, 36.46±1.09 and 67.10±1.17 mg GAE/g sample of total phenolics and 17.61±0.70 and 35.89±1.32 mg AAE/g sample of total antioxidant capacity for methanol and ethanol respectively. Ethanolic extract of whole finger millet scavenged above 50% hyrogen peroxide, NO and DPPH radicals. Hence, it terminated up to 8.97-65.16% lipid peroxidation reaction chain that may result from auto-oxidation, photo-oxidation and any other oxidation stress that may occur in plant and animal tissues. Therefore, pharmocology attention has to be given to finger millet which showed an effective antioxdants and antiradical capacities. Also, cultivation of finger millet should be encouraged by farmers.

scholarly journals Antioxidant-Free Radical Scavenging of Some Euphorbiaceae Herbs

2011 ◽  
Vol 2 (1) ◽  
pp. 146
Author(s):  
Titik Sunarni ◽  
Fransiska Leviana
Keyword(s):  
Acetic Acid ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Ic50 Value ◽  
Scavenger Activity ◽  
Acetic Acid Water

In order to screen natural antioxidant, the research about antioxidant of some Euphobiaceae herbs, have been conducted. The air-dried herbs  of Euphorbia heterophylla L, Phyllanthus acidus (L.) Skeels, and Phyllanthus buxifolius Muell Arg were extracted with metanol. The obtained extract was concentrated and then suspended to produce n-hexane, ethyl acetat and aqueous fractions. Free radical scavenger activity against DPPH (1,1-diphenyl-2-pycrylhydrazyl) measured by spectrophotometric method  and the IC50 value was determined. The compounds of  active fraction had been identified by TLC method. All of the herbs showed activity as DPPH scavenger. Among these herbs, Euphorbia heterophylla L. and Phyllanthus buxifolius Muell, Arg. exhibited a strong free radical scavenging of ethyl acetat fraction with IC50 value 5,88 µg/ml and 4,64 µg/ml. The result of TLC by mobile phase n-buthanol-acetic acid-water (4:1:5) and acetic acid 15% showed flavonoid compound.Keywords: Euphorbiaceae herbs, antioxidant, DPPH, flavonoid

Investigation of Free Radical Scavenging Enzyme Activities and Lipid Peroxidation in Human Placental Tissues With Miscarriage

2006 ◽  
Vol 13 (5) ◽  
pp. 384-388 ◽  
Author(s):  
Aydan Biri ◽  
Mustafa Kavutcu ◽  
Nuray Bozkurt ◽  
Erdinç Devrim ◽  
Nilhan Nurlu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Enzyme Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging

scholarly journals Phytochemical screening and In-vitro antioxidant activity of Centella asiatica extracts

2017 ◽  
Vol 9 (4) ◽  
pp. 615
Author(s):  
Mukesh Kumar Yadav ◽  
Santosh Kumar Singh ◽  
JS Tripathi ◽  
YB Tripathi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Phytochemical Screening

<p><em>Centella asiatica</em> also known as <em>mandukparni </em>or Indian pennywort or <em>jalbrahmi</em>, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda. <em>Centella asiatica</em> has long been used to improve memory and cognitive function.</p><p>The study aimed to identify the phytochemicals present in different solvent extracts of <em>Centella asiatica </em>(i.e. PECA- Petroleum ether extract of <em>C. asiatica, </em>CCA- Chloroform extract of <em>C. asiatica, </em>EACA- Ethyl acetate extract of <em>C. asiatica,</em> ECA- Ethanolic extract of <em>C. asiatica, </em>HACA- Hydro-alcoholic extract of <em>C. asiatica</em>)<em> </em>and evaluate the respective in-vitro antioxidant potentials. <em></em></p><p>The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts of <em>Centella asiatica</em> was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.</p><p>In all different solvent extracts of <em>C. asiatica</em> revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with  EC<sub>50</sub> values for ECA=128.752±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC<sub>50</sub> values ECA=429.69±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity.</p><p> The present study indicates that the <em>Centella asiatica</em> extracts have good antioxidant activity which can be used in stress and anxiety and also a good source to be used as natural drugs.</p>

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