Bacterioplankton Activity in a Meso-eutrophic Subtropical Coastal Lagoon

The aim of this study was to investigate whether the bacterioplankton activity in the meso-eutrophic Conceição Lagoon would increase significantly under allochthonous inputs of inorganic nutrients and organic carbon. Abundance and biomass of bacterioplankton were evaluated under three treatments: light (14 h light/10 h dark), complete darkness (dark-control), and nutrient (C + N + P—dark, 100 : 10 : 1) enrichments during 72 h. Nutrient enrichments promoted a significant increase in abundance (maximum of 19.0 ×109 cells·L−1 in the first 32 hours) and biomass of the heterotrophic bacterioplankton, which induced the formation of large clusters. Bacterial biomass remained constant in the non-enriched incubations (dark-control and light). Bacterial growth rates were significantly higher after nutrient additions (1.35 d−1), followed by control (0.79 d−1), and light (0.63 d−1) treatments, which were statistically equal (p>0.05). Bacterial production rates were also significantly higher under nutrient additions (1.28 d−1), compared to the control and light (0.50 d−1 and 0.44 d−1, respectively), demonstrating that bacterial growth and production in this meso-eutrophic lagoon are under an immediate “bottom-up” regulation, followed by a potential top-down effect. These facts reinforce the urgency on improving the local wastewater management plan in order to prevent further expansion of anoxic waters.

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The Leucine Incorporation Method Estimates Bacterial Growth Equally Well in Both Oxic and Anoxic Lake Waters

Applied and Environmental Microbiology ◽

10.1128/aem.67.7.2916-2921.2001 ◽

2001 ◽

Vol 67 (7) ◽

pp. 2916-2921 ◽

Cited By ~ 24

Author(s):

David Bastviken ◽

Lars Tranvik

Keyword(s):

Bacterial Growth ◽

Bacterial Biomass ◽

Leucine Incorporation ◽

Anoxic Conditions ◽

Anoxic Waters ◽

Anoxic Environments ◽

Significant Difference ◽

Bacterial Growth Rates ◽

Lake Waters ◽

Incorporation Method

ABSTRACT Bacterial biomass production is often estimated from incorporation of radioactively labeled leucine into protein, in both oxic and anoxic waters and sediments. However, the validity of the method in anoxic environments has so far not been tested. We compared the leucine incorporation of bacterial assemblages growing in oxic and anoxic waters from three lakes differing in nutrient and humic contents. The method was modified to avoid O2 contamination by performing the incubation in syringes. Isotope saturation levels in oxic and anoxic waters were determined, and leucine incorporation rates were compared to microscopically observed bacterial growth. Finally, we evaluated the effects of O2 contamination during incubation with leucine, as well as the potential effects of a headspace in the incubation vessel. Isotope saturation occurred at a leucine concentration of above about 50 nM in both oxic and anoxic waters from all three lakes. Leucine incorporation rates were linearly correlated to observed growth, and there was no significant difference between oxic and anoxic conditions. O2 contamination of anoxic water during 1-h incubations with leucine had no detectable impact on the incorporation rate, while a headspace in the incubation vessel caused leucine incorporation to increase in both anoxic and O2-contaminated samples. The results indicate that the leucine incorporation method relates equally to bacterial growth rates under oxic and anoxic conditions and that incubation should be performed without a headspace.

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San Francisco's Vision for the Future: An Integrated Urban Wastewater Management Plan

Water Practice & Technology ◽

10.2166/wpt.2009.003 ◽

2009 ◽

Vol 4 (1) ◽

Author(s):

Jonathan W. Loiacono ◽

Chu-Fei H. Ho ◽

Natalie V. Sierra ◽

Domènec Jolis ◽

Carolyn Chiu ◽

...

Keyword(s):

San Francisco ◽

Planning Process ◽

Rainwater Harvesting ◽

Control Plant ◽

Management Plan ◽

Urban Setting ◽

Wastewater Management ◽

Urban Watershed ◽

Aging Infrastructure ◽

The City

The City and County of San Francisco (“City”) embarked upon a 30-year master planning process in part prompted by public concerns related to the neighbourhood impacts of the Southeast Water Pollution Control Plant (SEP). The Sewer System Master Plan, as it is called, developed a long term Integrated Urban Watershed Management Plan for the City's treatment plants and collection system. This paper focuses on the planning framework and public input to the process, particularly as it relates to proposed changes to the SEP. The resulting improvements address issues of replacing aging infrastructure, eliminating odor emissions, and visually screening the treatment plants that are situated within an urban setting. The recommended project addresses the needed repair of the existing infrastructure; and proposes that the City move towards an integrated urban watershed approach, initially through localized rainwater harvesting and opportunistic water reclamation.

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Pollutant loads analysis of domestic wastewater and environmental accounting housekeeping (EAH) books of domestic wastewater in drainage areas of inner city rivers in Japan for the purpose of preparing domestic wastewater management plan of the developing countries

Proceedings of the Symposium on Global Environment ◽

10.2208/proge.13.77 ◽

2005 ◽

Vol 13 ◽

pp. 77-82

Author(s):

Yoshiaki Tsuzuki

Keyword(s):

Developing Countries ◽

Inner City ◽

Domestic Wastewater ◽

Management Plan ◽

Environmental Accounting ◽

Wastewater Management ◽

Pollutant Loads ◽

Loads Analysis

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Ecology of aerobic anoxygenic phototrophic bacteria along an oligotrophic gradient in the Mediterranean Sea

Biogeosciences ◽

10.5194/bg-8-973-2011 ◽

2011 ◽

Vol 8 (4) ◽

pp. 973-985 ◽

Cited By ~ 28

Author(s):

D. Lamy ◽

C. Jeanthon ◽

M. T. Cottrell ◽

D. L. Kirchman ◽

F. Van Wambeke ◽

...

Keyword(s):

Mediterranean Sea ◽

Bacterial Growth ◽

Growth Response ◽

Anoxygenic Phototrophic Bacteria ◽

Carbon Limitation ◽

Organic Substrates ◽

Western Basin ◽

Eastern Basin ◽

Nutrient Additions ◽

The Mediterranean

Abstract. Aerobic anoxygenic phototrophic (AAP) bacteria are photoheterotrophic prokaryotes able to use both light and organic substrates for energy production. They are widely distributed in coastal and oceanic environments and may contribute significantly to the carbon cycle in the upper ocean. To better understand questions regarding links between the ecology of these photoheterotrophic bacteria and the trophic status of water masses, we examined their horizontal and vertical distribution and the effects of nutrient additions on their growth along an oligotrophic gradient in the Mediterranean Sea. Concentrations of bacteriochlorophyll-a (BChl-a) and AAP bacterial abundance decreased from the western to the eastern basin of the Mediterranean Sea and were linked with concentrations of chlorophyll-a, nutrient and dissolved organic carbon. Inorganic nutrient and glucose additions to surface seawater samples along the oligotrophic gradient revealed that AAP bacteria were nitrogen- and carbon-limited in the ultraoligotrophic eastern basin. The intensity of the AAP bacterial growth response generally differed from that of the total bacterial growth response. BChl-a quota of AAP bacterial communities was significantly higher in the eastern basin than in the western basin, suggesting that reliance on phototrophy varied along the oligotrophic gradient and that nutrient and/or carbon limitation favors BChl-a synthesis.

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Role of bacterial growth rates in the epidemiology and pathogenesis of urinary infections in women.

Journal of Clinical Microbiology ◽

10.1128/jcm.10.6.766-771.1979 ◽

1979 ◽

Vol 10 (6) ◽

pp. 766-771 ◽

Cited By ~ 14

Author(s):

J D Anderson ◽

F Eftekhar ◽

M Y Aird ◽

J Hammond

Keyword(s):

Bacterial Growth ◽

Growth Rates ◽

Urinary Infections ◽

Bacterial Growth Rates

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Control of pollution in organized industrial districts: A case study from Turkey

Water Science & Technology ◽

10.2166/wst.1996.0319 ◽

1996 ◽

Vol 34 (12) ◽

pp. 127-133 ◽

Cited By ~ 1

Author(s):

Ayse Filibeli ◽

Füsun Sengül ◽

Aysen Müezzinoglu

Keyword(s):

Pollution Control ◽

Treatment Plant ◽

Management Plan ◽

Wastewater Management ◽

Industrial District ◽

Environmental Criteria ◽

Approximate Cost ◽

Optimized Treatment ◽

Control Study

At Manisa Organized Industrial District (OID) chosen as pilot study area, a detailed wastewater profile and pollution control study has been realized. A pretreatment strategy was determined and a wastewater management plan was prepared. Existing central treatment plant was studied and cost items were determined. These evaluations were compared with the redesign options generated from our own wastewater management plan. Same treatment units and their combinations were successively designed for variable flow rates between 100-50,000 m3/d. Thus data for new OIDs of similar nature as Manisa, an optimized treatment scheme as well as its approximate cost varying with the wastewater inflow was generated. Proposals for preparing environmental criteria to be considered and applied in newly establishing or planned OIDs are summarized.

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