scholarly journals Selective Recognition of Myoglobin in Biological Samples Using Molecularly Imprinted Polymer-Based Affinity Traps

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Rüstem Keçili
Keyword(s):  
Binding Capacity ◽  
Suspension Polymerization ◽  
Selective Recognition ◽  
Selective Detection ◽  
Imprinted Polymer ◽  
Serum Samples ◽  
Medium Ph ◽  
Molecularly Imprinted ◽  
Maximum Binding Capacity ◽  
Selectivity Studies

The current work demonstrates the design, characterization, and preparation of molecularly imprinted microspheres for the selective detection of myoglobin in serum samples. The suspension polymerization approach was applied for the preparation of myoglobin imprinted microspheres. For this purpose, N-methacryloylamino folic acid-Nd3+(MAFol- Nd3+) was chosen as the complex functional monomer. The optimization studies were performed changing the medium pH, temperature, and myoglobin concentration. pH 7.0 was determined as the optimum value where the prepared imprinted microspheres displayed maximum binding for myoglobin. The maximum binding capacity was achieved as 623 mgg−1. In addition, the selectivity studies were conducted. The results confirmed that the imprinted microspheres showed great selectivity towards myoglobin in the existence of hemoglobin, cytochrome c, and lysozyme which were chosen as potentially competing proteins.

Preparation of carbon-based metal organic frameworks modified molecularly imprinted polymers for selective recognition of bovine hemoglobin in biological samples

2022 ◽  
Author(s):  
Xue Chen ◽  
Jinyue Chai ◽  
Baodong Sun ◽  
Xue Yang ◽  
Feng Zhang ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Metal Organic Framework ◽  
Selective Recognition ◽  
Template Molecule ◽  
Bovine Hemoglobin ◽  
Carbon Spheres ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Metal Organic ◽  
Carbon Based

In this study, the carbon-based Cu2+-immobilized metal-organic framework modified molecularly imprinted polymer (C@GI@Cu-MOFs@MIPs) adsorbent was prepared using bovine hemoglobin (BHb) as a template molecule with carbon spheres as carriers for...

Molecularly Imprinted Polymer‐based Novel Electrochemical Sensor for the Selective Detection of Aldicarb

2020 ◽  
Vol 217 (9) ◽  
pp. 1900599
Author(s):  
Suryansh Saxena ◽  
Garimella B. V. S. Lakshmi ◽  
Deepika Chauhan ◽  
Pratima R. Solanki
Keyword(s):  
Electrochemical Sensor ◽  
Molecularly Imprinted Polymer ◽  
Selective Detection ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

scholarly journals Removal of bisphenol A from aqueous media using a highly selective adsorbent of hybridization cyclodextrin with magnetic molecularly imprinted polymer

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
S. Mamman ◽  
F. B. M. Suah ◽  
M. Raaov ◽  
F. S. Mehamod ◽  
S. Asman ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Molecularly Imprinted Polymer ◽  
Binding Capacity ◽  
Bulk Polymerization ◽  
Resonance Spectroscopy ◽  
Template Molecule ◽  
Order Kinetic ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Magnetic Molecularly Imprinted Polymer

In this study, a unique magnetic molecularly imprinted polymer (MMIP) adsorbent towards bisphenol A (BPA) as a template molecule was developed by bulk polymerization using β-cyclodextrin (β-CD) as a co-monomer with methacrylic acid (MAA) to form MMIP MAA–βCD as a new adsorbent. β-CD was hybridized with MAA to obtain water-compactible imprinting sites for the effective removal of BPA from aqueous samples. Benzoyl peroxide and trimethylolpropane trimethacrylate were used as the initiator and cross-linker, respectively. The adsorbents were characterized by Fourier transform infrared spectroscopy, scanning electronic microscopy, transmission electron microscopy, vibrating sample magnetometer, Brunauer–Emmett–Teller and X-ray diffraction. 1 H nuclear magnetic resonance spectroscopy was used to characterize the MAA–βCD and BPA–MAA–βCD complex. Several parameters influencing the adsorption efficiency of BPA such as adsorbent dosage, pH of sample solution, contact time, initial concentrations and temperature as well as selectivity and reusability study have been evaluated. MMIP MAA–βCD showed significantly higher removal efficiency and selective binding capacity towards BPA compared to MMIP MAA owing to its unique morphology with the presence of β-CD. The kinetics data can be well described by the pseudo second-order kinetic and Freundlich isotherm and Halsey models best fitted the isotherm data. The thermodynamic studies indicated that the adsorption reaction was a spontaneous and exothermic process. Therefore, MMIP based on the hybrid monomer of MAA–βCD shows good potential of a new monomer in molecularly imprinted polymer preparation and can be used as an effective adsorbent for the removal of BPA from aqueous solutions.

ZnO nanoparticles and β-cyclodextrin containing molecularly imprinted polymers for gravimetric sensing of very-low-density lipoprotein

2021 ◽  
Author(s):  
Amara Nasrullah ◽  
Sumaira Roshan ◽  
Usman Latif ◽  
Adnan Mujahid ◽  
Ghulam Mustafa ◽  
...  
Keyword(s):  
Human Serum ◽  
Zno Nanoparticles ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Clinical Importance ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Imprinted Polymer ◽  
Serum Samples ◽  
Molecularly Imprinted

Abstract The elevated level of very-low-density lipoprotein (VLDL) in the blood is associated with coronary heart disease; therefore, its detection is of significant clinical importance. In this work, molecularly imprinted polymer (MIP) layers fabricated with ZnO nanoparticles are developed for gravimetric sensing of VLDL. The use of methacrylic acid (MAA) and β-cyclodextrin (β-CD) as functional co-monomers in an optimized ratio of 1:1 for MIP synthesis controls the hydrophilicity/hydrophobicity; thus, yielding highly tailored recognition sites having adequate stability. The as-prepared ZnO nanoparticles are characterized by scanning electron microscopy (SEM), Fourier transformation infrared (FTIR), and X-ray diffraction (XRD) before incorporating into the MIP matrix. The template concentration in MIP is also varied to select its optimal amount, i.e., 50 µL of 50 µg/mL VLDL solution for enhanced sensor performance. Sensor measurements reveal that the ZnO-MIP has a sensitivity of 19.285 Hz.ng-1mL-1 for VLDL, which is about 16-fold higher than the reference ZnO-NIP (non-imprinted polymer) channel. Furthermore, the ZnO-MIP sensor exhibits high selectivity for VLDL as the sensor response is 6 and 3 times higher compared to α1-acid glycoprotein and human serum albumin (HSA), respectively. Finally, the performance of the developed sensor setup is evaluated for the detection of VLDL in human serum samples indicating its potential for reliable analysis of VLDL in complex biofluids.

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