Corrigendum to “Subchronic Infection of Porphyromonas gingivalis and Tannerella forsythia Stimulates an Immune Response but Not Arthritis in Experimental Murine Model”

Studies have proposed that Porphyromonas gingivalis (Pg) and Tannerella forsythia (Tf) promote a nonspecific inflammatory response that could produce systemic disease. Oral inoculation of Pg and Tf on the immune and arthritis response was evaluated in BALB/C mice divided into four groups: (1) sham; (2) food contaminated with Pg/Tf; (3) complete Freund’s adjuvant (CFA) + Pg/Tf; and (4) CFA alone. CFA was administered subcutaneously on days 1 and 14. The arthritis response was monitored for 21 days after day 14 of CFA administration. IL-1β and IL-6 were determined in serum. T cell activation was evaluated by CD25 in salivary lymph nodes or mouse spleen. Pad inflammation appeared by day 19 in the CFA group, but animals with bacteria inoculation presented a delay. A significant increase in IL-6 was found in Groups 3 and 4, but not with respect to IL-1β. We observed an increase in CD25 in cells derived from cervical nodes and in animals with bacteria inoculation and CFA. A local immune response was observed in mice inoculated with Pg and Tf (T cell activation); a systemic response was observed with CFA. Since pad inflammation was delayed by bacterial inoculation this suggests that local T cell activation could decrease pad inflammation.

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Persimmon-derived tannin ameliorates the pathogenesis of ulcerative colitis in a murine model through inhibition of the inflammatory response and alteration of microbiota

Scientific Reports ◽

10.1038/s41598-021-86608-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Masahiro Kitabatake ◽

Yoko Matsumura ◽

Noriko Ouji-Sageshima ◽

Tatsuki Nishioka ◽

Atsushi Hara ◽

...

Keyword(s):

Ulcerative Colitis ◽

Immune Response ◽

Murine Model ◽

Control Diet ◽

Antimicrobial Activities ◽

Diet Group ◽

Chronic Inflammatory Bowel Disease ◽

Diospyros Kaki ◽

Colon Inflammation

AbstractUlcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) induced by dysregulation of the immune response in the intestinal mucosa. Although the underlying mechanisms of UC development are not fully understood, disruption of gut microbiota, “dysbiosis”, is thought to lead to the development of IBD. Persimmon (Ebenaceae Diospyros kaki Thunb.)-derived tannin, which is a condensed polymeric tannin consisting of catechin groups, has antioxidant, anti-inflammatory, and antimicrobial activities. In this study, we assessed the effect of persimmon-derived tannin on a murine model of UC established by dextran sulfate sodium-induced colitis in female mice. Dietary supplementation of tannin significantly decreased disease activity and colon inflammation. A hydrolysate of tannin directly suppressed expression of inflammatory genes in macrophages in vitro. In faecal microbiota, the relative abundance of Bacteroides was increased significantly by tannin supplementation. Alpha-diversity indices in colitis-induced mice were significantly higher in the tannin diet group compared with the control diet group. Additionally, expansion of Enterobacteriaceae and Enterococcus, which is associated with disease progression of IBD, was remarkably suppressed in the tannin diet group. These results suggest that persimmon-derived tannin ameliorates colon inflammation in UC through alteration of the microbiota composition and immune response, which may be a promising candidate for IBD therapy.

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Balanced Th1/Th2 immune response induced by MSP1a functional motif coupled to multiwalled carbon nanotubes as anti-anaplasmosis vaccine in murine model

Nanomedicine Nanotechnology Biology and Medicine ◽

10.1016/j.nano.2019.102137 ◽

2020 ◽

Vol 24 ◽

pp. 102137 ◽

Cited By ~ 2

Author(s):

Leticia Santos Pimentel ◽

Carolina Alvarenga Turini ◽

Paula Souza Santos ◽

Mariana Abilio de Morais ◽

Aline Gomes Souza ◽

...

Keyword(s):

Carbon Nanotubes ◽

Immune Response ◽

Multiwalled Carbon Nanotubes ◽

Murine Model ◽

Multiwalled Carbon ◽

Functional Motif ◽

Th2 Immune Response

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Protective Activity of S-PT84, a Heat-killed Preparation of Lactobacillus pentosus, against Oral and Gastric Candidiasis in an Experimental Murine Model

Medical Mycology Journal ◽

10.3314/mmj.55.j123 ◽

2014 ◽

Vol 55 (3) ◽

pp. J123-J129 ◽

Cited By ~ 6

Author(s):

Kazumi Hayama ◽

Sanae Ishijima ◽

Yoshiko Ono ◽

Takayuki Izumo ◽

Masayuki Ida ◽

...

Keyword(s):

Murine Model ◽

Protective Activity ◽

Lactobacillus Pentosus ◽

Experimental Murine Model

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Chitosan gel prevents the growth of Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola in mini-implant during orthodontic treatment

The Saudi Dental Journal ◽

10.1016/j.sdentj.2021.06.003 ◽

2021 ◽

Author(s):

Haru Setyo Anggani ◽

Victoria Rusli ◽

Endang W Bachtiar

Keyword(s):

Porphyromonas Gingivalis ◽

Orthodontic Treatment ◽

Treponema Denticola ◽

Tannerella Forsythia ◽

Chitosan Gel

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Nimbolide ameliorates fibrosis and inflammation in experimental murine model of bleomycin-induced scleroderma

Inflammopharmacology ◽

10.1007/s10787-018-0527-4 ◽

2018 ◽

Vol 27 (1) ◽

pp. 139-149 ◽

Cited By ~ 2

Author(s):

Snehalatha Diddi ◽

Swarna Bale ◽

Gauthami Pulivendala ◽

Chandraiah Godugu

Keyword(s):

Murine Model ◽

Experimental Murine Model

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Viremia in Equine Herpes Virus-1 infection and a possible link to Transient Protective Immunity

Journal of Human Virology & Retrovirology ◽

10.15406/jhvrv.2021.09.00238 ◽

2021 ◽

Vol 9 (1) ◽

pp. 11-16

Author(s):

AR Awan ◽

OL Tulp ◽

HJ Field

Keyword(s):

Immune Response ◽

Immune Responses ◽

Murine Model ◽

Herpes Virus ◽

Natural Infection ◽

Fluorescent Antibody ◽

Buffy Coat ◽

Effective Vaccine ◽

Infection Model ◽

Herpès Virus

Equine herpes virus (EHV-1) causes respiratory infections in equine, and results in abortion, paresis, neonatal death, and retinopathy and the virus may become latent following initial infection. Virus entry is via the respiratory route, and the virus replicates in the host in ciliated and non-ciliated epithelial cells of the respiratory tract and in Type 1 and Type 2 pneumocytes in the lung parenchyma. After viral replication in the respiratory system, the virus can become disseminated to other parts of body via viraemic cells. The virus also can cross the placenta which leads to abortion of live or dead fetuses without premonitory signs. Infected horses show transient immunity after natural or experimental infection and immune responses to EHV-1, but the immunoprotective status begins to decline after a few months of active infection. Due to the transient immune response, recovered horses are not immunoprotected and thus are prone to subsequent re-infection. Immunity is not long lived after experimental or natural infection, and as a result the development of an effective vaccine has remained a challenge. In this study viraemic cells were studied in a murine EHV-1 infection model. Mice were infected intranasally and viraemic cells were studied on days three and five which occurs during the peak of the infection. The results of this study may help to identify the nature of viraemic cells and their role in the transient immune response to infection. Buffy coat cells and lungs were removed and stained with a fluorescent antibody test for EHV-1 antigen, and lung specimens were subjected to transmission electron microscopy. Both techniques confirmed the presence of viraemic cells in lung tissues. These viraemic cells were further stained for EHV-1 antigen, and for CD4 or CD8 biomarkers and results are discussed re: pathogenesis of EHV-1 infection, identification of viraemic cells in a murine model and possible link of viraemia to transient immune responses in EHV-1 infection, which demonstrate the validity of this murine model for the investigation of the cytopathologic mechanism and sequelae of EHV manifestation in this model.

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An Investigation Into The Synergistic Relationship Between Lactoferrin And Azithromycin With Particular Reference To Periodontopathic Bacteria

IIUM Medical Journal Malaysia ◽

10.31436/imjm.v16i2.1047 ◽

2017 ◽

Vol 16 (2) ◽

Author(s):

Juzaily Husain

Keyword(s):

Protein Synthesis ◽

Outer Membrane ◽

Porphyromonas Gingivalis ◽

Treatment Strategies ◽

Antimicrobial Protein ◽

Tannerella Forsythia ◽

Iron Depletion ◽

Test Strips ◽

Agar Diffusion ◽

Synergistic Relationship

Introduction: The development of treatment strategies for periodontitis that maximise the effectiveness of antibiotics is highly desirable. Azithromycin is proving to be an effective antibiotic for treatment of refractory periodontitis which works by binding to the outer membrane of Gramnegative bacteria and subsequently inhibits protein synthesis. Lactoferrin is a membrane-active host antimicrobial protein and so the objective of this study was to determine whether the effect of azithromycin (AZM) against example periodontopathogens (Porphyromonas gingivalis and Tannerella forsythia) could be potentiated by lactoferrin. Materials and Methods: Two strains of P. gingivalis and T. forsythia were exposed to lactoferrin (LF; up to 10 mg/ml) and AZM (up to 5 g/ml) for 0 -72 h. The MICs for AZM were established using E-Test strips and by agar diffusion. Susceptibility to LF and LF + AZM was evaluated using diffusion assays, with and without iron depletion. Results: The range of MIC values of AZM for P. gingivalis strains and T. forsythia was 0.16 - 0.63 µg/ml and 0.50 - 0.63 µg/ml, respectively. However, no inhibition was observed with iron saturated lactoferrin at any concentration or under iron depletion conditions nor was any effect observed on the AZM MIC by its presence. Conclusion(s): P. gingivalis and T. forsythia were inhibited by AZM but were not affected by LF and there was no synergism between AZM and LF.

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