Isolation and Characterization of Neural Stem Cells from the Rat Inferior Colliculus

The inferior colliculus (IC) is a nucleus of the auditory pathway and its fourth relay station. It integrates afferent information from the superior olivary complex and the cochlear nucleus. To date, no causal therapeutic options are known for damaged neuronal structures in this area. Regenerative medicine offers a potential approach to causally treating hearing impairment. After neural stem cells had been identified in certain areas of the auditory pathway, the question arouses, whether the IC also has a neurogenic potential. Cells from the IC of postnatal day 6 rats were extracted and cultured as neurospheres. Cells in the neurospheres showed mitotic activity and positive stain of neural stem cell markers (Nestin, DCX, Atoh1, and Sox-2). In addition, single cells were differentiated into neuronal and glial cells shown by the markers β-III-tubulin, GFAP, and MBP. In summary, basic stem cell criteria could be detected and characterized in cells isolated from the IC of the rat. These findings will lead to a better understanding of the development of the auditory pathway and may also be relevant for identifying causal therapeutic approaches in the future.

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Isolation and Characterization of Human Epidermal Stem Cells

Clinical Science ◽

10.1042/cs0910141 ◽

1996 ◽

Vol 91 (2) ◽

pp. 141-146 ◽

Cited By ~ 24

Author(s):

P. H. Jones

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Matrix Proteins ◽

Stem Cell Markers ◽

Epidermal Stem Cells ◽

Cell Behaviour ◽

Isolation And Characterization ◽

Integrin Family

1. The keratinocytes in human epidermis are constantly turned over and replaced by a population of stem cells located in the basal epidermal layer. Until recently there were no markers allowing the isolation of viable epidermal stem cells. However, it has now been shown that epidermal stem cells can be isolated both in vitro and direct from the epidermis as they express high levels of functional β1 integrin family receptors for extracellular matrix proteins. 2. The evidence for integrins as stem cell markers and the insights that have been gained into stem cell behaviour are reviewed.

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Isolation and Characterization of a Population of Immature Dental Pulp Stem Cells Expressing OCT-4 and Other Embryonic Stem Cell Markers

Cells Tissues Organs ◽

10.1159/000099617 ◽

2006 ◽

Vol 184 (3-4) ◽

pp. 105-116 ◽

Cited By ~ 264

Author(s):

Irina Kerkis ◽

Alexandre Kerkis ◽

Dmitri Dozortsev ◽

Gaëlle Chopin Stukart-Parsons ◽

Sílvia Maria Gomes Massironi ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Embryonic Stem Cell ◽

Dental Pulp ◽

Embryonic Stem ◽

Stem Cell Markers ◽

Cell Markers ◽

Isolation And Characterization ◽

Embryonic Stem Cell Markers

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Derivation and Characterization of EGFP-Labeled Rabbit Limbal Mesenchymal Stem Cells and Their Potential for Research in Regenerative Ophthalmology

Biomedicines ◽

10.3390/biomedicines9091134 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1134

Author(s):

Julia I. Khorolskaya ◽

Daria A. Perepletchikova ◽

Daniel V. Kachkin ◽

Kirill E. Zhurenkov ◽

Elga I. Alexander-Sinkler ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Fluorescent Protein ◽

Rabbit Model ◽

Stem Cell Markers ◽

Epithelial Stem Cells ◽

Limbal Stem Cell ◽

Green Fluorescent

The development of cell-based approaches to the treatment of various cornea pathologies, including limbal stem cell deficiency (LSCD), is an area of current interest in regenerative biomedicine. In this context, the shortage of donor material is urgent, and limbal mesenchymal stem cells (L-MSCs) may become a promising cell source for the development of these novel approaches, being established mainly within the rabbit model. In this study, we obtained and characterized rabbit L-MSCs and modified them with lentiviral transduction to express the green fluorescent protein EGFP (L-MSCs-EGFP). L-MSCs and L-MSCs-EGFP express not only stem cell markers specific for mesenchymal stem cells but also ABCG2, ABCB5, ALDH3A1, PAX6, and p63a specific for limbal epithelial stem cells (LESCs), as well as various cytokeratins (3/12, 15, 19). L-MSCs-EGFP have been proven to differentiate into adipogenic, osteogenic, and chondrogenic directions, as well as to transdifferentiate into epithelial cells. The possibility of using L-MSCs-EGFP to study the biocompatibility of various scaffolds developed to treat corneal pathologies was demonstrated. L-MSCs-EGFP may become a useful tool for studying regenerative processes occurring during the treatment of various corneal pathologies, including LSCD, with the use of cell-based technologies.

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Isolation and characterization of neural stem cells from fetal canine spinal cord

Neuroscience Letters ◽

10.1016/j.neulet.2021.136293 ◽

2021 ◽

Vol 765 ◽

pp. 136293

Author(s):

Sarah Ingrid Pinto Santos ◽

Vanessa Cristina de Oliveira ◽

Naira Caroline Godoy Pieri ◽

Fabiana Fernandes Bressan ◽

Carlos Eduardo Ambrósio ◽

...

Keyword(s):

Spinal Cord ◽

Stem Cells ◽

Neural Stem Cells ◽

Isolation And Characterization

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Isolation and characterization of neural stem cells from dystrophic mdx mouse

Experimental Cell Research ◽

10.1016/j.yexcr.2016.03.019 ◽

2016 ◽

Vol 343 (2) ◽

pp. 190-207 ◽

Cited By ~ 4

Author(s):

Tiziana Annese ◽

Patrizia Corsi ◽

Simona Ruggieri ◽

Roberto Tamma ◽

Christian Marinaccio ◽

...

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Mdx Mouse ◽

Isolation And Characterization

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Differentiation of Human Breast-Milk Stem Cells to Neural Stem Cells and Neurons

Neurology Research International ◽

10.1155/2014/807896 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 30

Author(s):

Seyed Mojtaba Hosseini ◽

Tahere Talaei-khozani ◽

Mahsa Sani ◽

Bahareh Owrangi

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Growth Factor ◽

Breast Milk ◽

Neural Stem Cells ◽

Human Breast Milk ◽

Stem Cell Markers ◽

Human Breast ◽

Fetal Bovine ◽

Endothelial Growth Factor

Objectives.Human breast milk contains a heterogeneous population of cells that have the potential to provide a noninvasive source of cells for cell therapy in many neurodegenerative diseases without any ethical concern. The objectives of this study were to differentiate the breast milk-derived stem cells (BMDSC) toward neural stem cells and then into the neurons and neuroglia.Materials and Methods.To do this, the BMDSC were isolated from human breast milk and cultured in Dulbecco’s modified Eagle medium/F12 (DMEM/F12) containing fibroblast growth factor (bFGF). The cells were then characterized by evaluation of the embryonic and stem cell markers. Then, the cells were exposed to culture medium containing 1% B27 and 2% N2 for 7–10 days followed by medium supplemented with B27, N2, bFGF 10 µg/mL, and endothelial growth factor (EGF) 20 µg/mL. Then, the sphere-forming assay was performed. The spheres were then differentiated into three neural lineages by withdrawing growth factor in the presence of 5% FBS (fetal bovine serum). The immunofluorescence was done forβ-tubulin III, O4, and GFAP (glial fibrillary acidic protein).Results.The results indicated that the cells expressed both embryonic and mesenchymal stem cell (MSC) markers. They also showed neurospheres formation that was nestin-positive. The cells were also differentiated into all three neural lineages.Conclusion.The BMDSC can behave in the same way with neural stem cells. They were differentiated into oligodendrocytes, and astrocytes as well as neurons.

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Isolation and characterization of neural stem cells from the neonatal rat cochlear nucleus

Cell and Tissue Research ◽

10.1007/s00441-010-1118-x ◽

2011 ◽

Vol 343 (3) ◽

pp. 499-508 ◽

Cited By ~ 13

Author(s):

Kristen Rak ◽

Natalia V. Wasielewski ◽

Andreas Radeloff ◽

Johannes Völkers ◽

Agmal Scherzed ◽

...

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Cochlear Nucleus ◽

Neonatal Rat ◽

Isolation And Characterization

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Stem Cells and the Endometrium: From the Discovery of Adult Stem Cells to Pre-Clinical Models

Cells ◽

10.3390/cells10030595 ◽

2021 ◽

Vol 10 (3) ◽

pp. 595

Author(s):

Lucía de de Miguel-Gómez ◽

Sara López-Martínez ◽

Emilio Francés-Herrero ◽

Adolfo Rodríguez-Eguren ◽

Antonio Pellicer ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Adult Stem Cells ◽

Stem Cell Markers ◽

Cell Therapies ◽

Lineage Differentiation ◽

Functional Methods ◽

Isolation And Characterization ◽

Clinical Models ◽

Therapeutic Alternatives

Adult stem cells (ASCs) were long suspected to exist in the endometrium. Indeed, several types of endometrial ASCs were identified in rodents and humans through diverse isolation and characterization techniques. Putative stromal and epithelial stem cell niches were identified in murine models using label-retention techniques. In humans, functional methods (clonogenicity, long-term culture, and multi-lineage differentiation assays) and stem cell markers (CD146, SUSD2/W5C5, LGR5, NTPDase2, SSEA-1, or N-cadherin) facilitated the identification of three main types of endogenous endometrial ASCs: stromal, epithelial progenitor, and endothelial stem cells. Further, exogenous populations of stem cells derived from bone marrow may act as key effectors of the endometrial ASC niche. These findings are promoting the development of stem cell therapies for endometrial pathologies, with an evolution towards paracrine approaches. At the same time, promising therapeutic alternatives based on bioengineering have been proposed.

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Evaluation of the Neurogenic Potential in the Rat Inferior Colliculus from Early Postnatal Days Until Adulthood

Molecular Neurobiology ◽

10.1007/s12035-020-02151-6 ◽

2020 ◽

Author(s):

Jonas Engert ◽

Kristen Rak ◽

Linda Bieniussa ◽

Miriam Scholl ◽

Rudolf Hagen ◽

...

Keyword(s):

Stem Cells ◽

Glial Fibrillary Acidic Protein ◽

Inferior Colliculus ◽

Basic Protein ◽

Single Cells ◽

Auditory Pathway ◽

Neural Lineage ◽

Qrt Pcr ◽

Histological Sections ◽

Lineage Markers

Abstract Neural stem cells (NSCs) have been recently identified in the inferior colliculus (IC). These cells are of particular interest, as no casual therapeutic options for impaired neural structures exist. This research project aims to evaluate the neurogenic potential in the rat IC from early postnatal days until adulthood. The IC of rats from postnatal day 6 up to 48 was examined by neurosphere assays and histological sections. In free-floating IC cell cultures, neurospheres formed from animals from early postnatal to adulthood. The amount of generated neurospheres decreased in older ages and increased with the number of cell line passages. Cells in the neurospheres and the histological sections stained positively with NSC markers (Doublecortin, Sox-2, Musashi-1, Nestin, and Atoh1). Dissociated single cells from the neurospheres differentiated and were stained positively for the neural lineage markers β-III-tubulin, glial fibrillary acidic protein, and myelin basic protein. In addition, NSC markers (Doublecortin, Sox-2, CDK5R1, and Ascl-1) were investigated by qRT-PCR. In conclusion, a neurogenic potential in the rat IC was detected and evaluated from early postnatal days until adulthood. The identification of NSCs in the rat IC and their age-specific characteristics contribute to a better understanding of the development and the plasticity of the auditory pathway and might be activated for therapeutic use.

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