Effects of In Ovo Injection of Coenzyme Q10 on Hatchability, Subsequent Performance, and Immunity of Broiler Chickens

Effects of in ovo injection of Q10 on hatchability, performance (feed intake (FI), body weight gain (BWG), feed/gain ratio (F/G)) traits, and immune status of Ross × Ross 308 broiler chicks, hatched from eggs laid by a 38-week-old breeder flock, were determined through 42 days after hatch. Eggs containing live embryos were injected in the amnion with 0.1 and 0.2 mL Q10 solution on day 18 of incubation. Two controls groups were included as sham and/or as an uninjected group. At 28 and 42 days of age, performance traits, serum enzyme activity, weights of immune organs, and serum antibody titer of viral diseases were determined. Results were shown that hatchability % increased by Q10 on average of 6.54% (P≤0.025) and body weight/egg weight after hatching increased up to 4.74% (P≤0.002), compared with uninjected and sham controls. Injection of Q10 at different levels led to significant increases (P≤0.001) in performance traits all over the rearing period (P<0.05). Weight of immune organs significantly improved compared to uninjected and sham controls (P<0.05). In addition, serum antibody titers of viral diseases as well as serum enzyme activity of AST, ALT, CAT, and SOD were significantly changed by Q10 treated groups than controls (P≤0.01). In conclusion, in ovo injection of Q10 at levels of 0.1 and 0.2 mL led to significant increases in hatchability%, internal egg characteristics, and performance parameters as well as serum enzyme activity, weight of immune organs, and serum antibody titer of ND, AI, and IBD diseases.

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Streptokinase and serum enzyme activity in myocardial infarction.

BMJ ◽

10.1136/bmj.1.6067.1005 ◽

1977 ◽

Vol 1 (6067) ◽

pp. 1005-1005 ◽

Cited By ~ 3

Author(s):

F Praetorius

Keyword(s):

Myocardial Infarction ◽

Enzyme Activity ◽

Serum Enzyme ◽

Serum Enzyme Activity

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Anti-diabetic properties of root extracts of Ruellia tuberosa L: effects on serum enzyme activity

Journal of Physics Conference Series ◽

10.1088/1742-6596/1374/1/012030 ◽

2019 ◽

Vol 1374 ◽

pp. 012030 ◽

Cited By ~ 1

Author(s):

A Safitri ◽

A Roosdiana ◽

N Arrochmah ◽

S S Nur’Adya

Keyword(s):

Enzyme Activity ◽

Serum Enzyme ◽

Serum Enzyme Activity ◽

Root Extracts

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Effect of In Ovo Injection of L-Arginine in Different Chicken Embryonic Development Stages on Post-Hatchability, Immune Response, and Myo-D and Myogenin Proteins

Animals ◽

10.3390/ani9060357 ◽

2019 ◽

Vol 9 (6) ◽

pp. 357 ◽

Cited By ~ 6

Author(s):

Sivakumar Allur Subramaniyan ◽

Da Rae Kang ◽

Jin Ryong Park ◽

Sharif Hasan Siddiqui ◽

Palanisamy Ravichandiran ◽

...

Keyword(s):

Immune Response ◽

Body Weight ◽

Developmental Stages ◽

Muscle Growth ◽

In Ovo ◽

Serum Glutamate Pyruvate Transaminase ◽

In Ovo Injection ◽

Shock Proteins ◽

Related Proteins ◽

Serum Glutamate

The aim of this study was to evaluate the effect of in ovo injection with different ratios of L-arginine (L-Arg) into Ross broiler eggs at three different embryonic developmental stages (eighth day (d), 14th day, and 18th day) on the survival, hatchability, and body weight (BW) of one-day-old hatched chicks. Additionally, we have analyzed the levels of serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT), the protein expression of heat shock proteins (HSPs), and we have also determined micronuclei (MN) and nuclear abnormality (NA). In addition, the genotoxic effect was observed in peripheral blood cells such as the presence of micronuclei and nuclear abnormalities in the experimental groups. The results showed that survival and hatching rates as well as body weight were increased on the 14th day of incubation compared to the eighth and 18th day of incubation at lower concentrations of L-Arg. Moreover, the levels of SGOT and SGPT were also significantly (p < 0.05) increased on the 14th day of incubation at the same concentration (100 μg/μL/egg) of injection. In addition, immunoglobulin (IgM) levels were increased on the 14th day of incubation compared to other days. The protein expressions of HSP-47, HSP-60, and HSP-70 in the liver were significantly down-regulated, whereas the expression of myogenin and myoblast determination protein (MyoD) were significantly up-regulated on the 14th day after incubation when treated with all different doses such as 100 μg, 1000 μg, and 2500 μg/μL/egg, namely 3T1, 3T2, and 3T3, respectively. However, the treatment with low doses of L-Arg down-regulated the expression levels of those proteins on the 14th day of incubation. Histopathology of the liver by hematoxylin and eosin (H&E) staining showed that the majority of liver damage, specifically intracytoplasmic vacuoles, were observed in the 3T1, 3T2, and 3T3 groups. The minimum dose of 100 μg/mL/egg on the 14th day of incubation significantly prevented intracytoplasmic vacuole damages. These results demonstrate that in ovo administration of L-Arg at (100 μg/μL/egg) may be an effective method to increase chick BW, hatch rate, muscle growth-related proteins, and promote the immune response through increasing IgM on the 14th day of the incubation period.

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Relationship between Serum Enzyme Activity in Acute Myocardial Infarction and Morbidity during a 2-Year Follow-Up

Cardiology ◽

10.1159/000173988 ◽

1986 ◽

Vol 73 (2) ◽

pp. 85-93 ◽

Cited By ~ 4

Author(s):

J. Herlitz ◽

I. Helgesson ◽

B.M. Hjalmarson ◽

Å. Hjalmarson ◽

C. Jonsteg ◽

...

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Enzyme Activity ◽

Serum Enzyme ◽

Serum Enzyme Activity

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Prognosis in Patients with ST-T Changes but No Rise in Serum Enzyme Activity as Compared with Non-Q-Wave Infarction

Cardiology ◽

10.1159/000174890 ◽

1991 ◽

Vol 79 (4) ◽

pp. 271-279 ◽

Cited By ~ 6

Author(s):

Björn W. Karlson ◽

Johan Herlitz ◽

Arina Richter ◽

Magareta Sjölin ◽

Åke Hjalmarson

Keyword(s):

Enzyme Activity ◽

Serum Enzyme ◽

Serum Enzyme Activity ◽

Q Wave

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Protective Immunity against Eimeria acervulina following In Ovo Immunization with a Recombinant Subunit Vaccine and Cytokine Genes

Infection and Immunity ◽

10.1128/iai.72.12.6939-6944.2004 ◽

2004 ◽

Vol 72 (12) ◽

pp. 6939-6944 ◽

Cited By ~ 83

Author(s):

Xicheng Ding ◽

Hyun S. Lillehoj ◽

Marco A. Quiroz ◽

Erich Bevensee ◽

Erik P. Lillehoj

Keyword(s):

Body Weight ◽

Weight Gain ◽

Body Weight Gain ◽

Interleukin 1 ◽

Serum Antibody ◽

Intestinal Immunity ◽

In Ovo ◽

Eimeria Acervulina ◽

Genes Encoding ◽

Ifn Γ

ABSTRACT A purified recombinant protein from Eimeria acervulina (3-1E) was used to vaccinate chickens in ovo against coccidiosis both alone and in combination with expression plasmids encoding the interleukin 1 (IL-1), IL-2, IL-6, IL-8, IL-15, IL-16, IL-17, IL-18, or gamma interferon (IFN-γ) gene. When used alone, vaccination with 100 or 500 μg of 3-1E resulted in significantly decreased oocyst shedding compared with that in nonvaccinated chickens. Simultaneous vaccination of the 3-1E protein with the IL-1, -15, -16, or -17 gene induced higher serum antibody responses than 3-1E alone. To evaluate protective intestinal immunity, vaccinated birds were challenged with live E. acervulina oocysts 14 days posthatch, and fecal-oocyst shedding and body weight gain were determined as parameters of coccidiosis. Chickens vaccinated with 3-1E protein showed significantly lower oocyst shedding and normal body weight gain than nonvaccinated and infected controls. Simultaneous immunization with 3-1E and the IL-2, -15, -17, or -18 or IFN-γ gene further reduced oocyst shedding compared with that achieved with 3-1E alone. These results provide the first evidence that in ovo vaccination with the recombinant 3-1E Eimeria protein induces protective intestinal immunity against coccidiosis, and this effect was enhanced by coadministration of genes encoding immunity-related cytokines.

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