Functionalized Scaffold and Barrier Membrane with Anti-BMP-2 Monoclonal Antibodies for Alveolar Ridge Preservation in a Canine Model

Introduction. The aim of this study was to investigate the ability of anti-bone morphogenetic protein 2 monoclonal antibody (anti-BMP-2 mAb) to functionalize scaffolds to mediate bone regeneration in a canine model. Materials and Methods. The mandibular right premolar 4 (PM4) was extracted in eight beagle dogs and grafted with anti-BMP-2 mAb+anorganic bovine bone mineral with 10% collagen (ABBM-C) and porcine bilayer native collagen membrane (CM). The ABBM-C and CM were functionalized with either anti-BMP-2 mAb (test group) or an isotype matched control mAb (control group). Animals were euthanized at 12 weeks for radiographic, histologic, and histomorphometric analyses. Outcomes were compared between groups. Results. 3D imaging using cone beam computed tomography (CBCT) revealed that sites treated with ABBM-C and CM functionalized with anti-BMP-2 mAb exhibited significantly more remaining bone width near the alveolar crest, as well as buccal bone height, compared with control groups. Histologic and histomorphometric analyses demonstrated that in anti-BMP-2 mAb-treated sites, total tissue volume was significantly higher in the coronal part of the alveolar bone crest compared with control sites. In anti-BMP-2 mAb-treated sites, bone formation was observed under the barrier membrane. Conclusion. Functionalization of the ABBM-C scaffold and CM appeared to have led to bone formation within healing alveolar bone sockets.

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Pool of bovine morphogenetic proteins and guided tissue regeneration in the treatment of intrabony periodontal defects: I- Clinical measurements

Journal of Applied Oral Science ◽

10.1590/s1678-77572004000100013 ◽

2004 ◽

Vol 12 (1) ◽

pp. 70-77 ◽

Cited By ~ 8

Author(s):

Maria do Carmo Machado Guimarães ◽

Euloir Passanezi ◽

Adriana Campos Passanezi Sant'Ana ◽

Sebastião Luiz Aguiar Greghi

Keyword(s):

Demineralized Bone Matrix ◽

Bone Matrix ◽

Test Group ◽

Control Group ◽

Collagen Membrane ◽

Bovine Bone ◽

Intrabony Defects ◽

Bovine Collagen ◽

Barrier Membrane ◽

Clinical Measurements

The purpose of this study was to evaluate the effect of the pool of bovine BMPs on the treatment of intrabony defects. The sample comprised 15 patients aged 26 to 57 years old presenting with 10 pairs of lesions of 2 or 3 walls or 2-3 walls ³5mm, located in the same type teeth (premolar or molar) and same jaw. The test defects were treated with combination of a pool of bovine bone morphogenetic and resorbable hydroxyapatite carrier (BMPs- HA), bovine demineralized bone matrix (MB) and coverage by a bovine collagen barrier membrane. The control defects were treated with MB-HA and covered by a bovine collagen membrane. The clinical measurements at six months after therapy in the test group revealed a reduction in the mean probing pocket depth (PPD) of 1.63 ± 1.41mm (B) and 1.93 ± 0.96mm (L) and a mean change in the clinical attachment level (CAL) of 1.60 ± 1.16mm (B) and 1.46 ± 0.97mm (L). The control group showed a mean reduction of PPD of 1.93 ± 1.34mm (B) and 2.0 ± 1.51mm (L) and a mean change of CAL of 1.03 ± 1.24mm (B) and 1.30 ± 1.14 mm (L). The analysis of variance (ANOVA) demonstrated that the changes in the clinical parameters were statistically significant (p<0.05). There were no significant differences between the test and controls subjects (Student's test, p<0.005). These findings suggest that the use of a pool of bovine BMPs do not provide added effects to GTR in the treatment of intrabony defects.

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Use of Platelet-Rich Fibrin Associated with Xenograft in Critical Bone Defects: Histomorphometric Study in Rabbits

Symmetry ◽

10.3390/sym11101293 ◽

2019 ◽

Vol 11 (10) ◽

pp. 1293

Author(s):

Paulo Wilson Maia ◽

Marcelo Lucchesi Teixeira ◽

Luís Guilherme Scavone de Macedo ◽

Antonio Carlos Aloise ◽

Celio Amaral Passos Junior ◽

...

Keyword(s):

Bone Formation ◽

Saline Solution ◽

Healing Process ◽

Test Group ◽

The Other ◽

Control Group ◽

Collagen Membrane ◽

Platelet Rich Fibrin ◽

Histomorphometric Study ◽

Significant Difference

Platelet-rich fibrin (PRF) is an autologous material used to improve bone regeneration when associated with bone grafts. It affects tissue angiogenesis, increasing the healing process and, theoretically, presenting potential to increase bone neoformation. The aim of this study was to verify, histomorphometrically, the effects of the association of PRF to a xenograft. Twelve adult white New Zealand rabbits were randomly assigned into two groups containing six animals each. After general anesthesia of the animals, two critical defects of 12 mm were created in the rabbit calvaria, one on each side of the sagittal line. Each defect was filled with the following biomaterials: in the control group (CG), xenograft hydrated with saline solution filling one defect and xenograft hydrated with saline solution covered with collagen membrane on the other side; in the test group (TG), xenograft associated with PRF filling the defect of one side and xenograft associated with PRF covered with collagen membrane on the other side. After eight weeks the animals were euthanized and a histomorphometric analysis was performed. The results showed that in the sites that were covered with collagen membrane, there was no statistically significant difference for all the analyzed parameters. However, when comparing the groups without membrane coverage, a statistically significant difference could be observed for the vital mineralized tissue (VMT) and nonmineralized tissue (NMT) parameters, with more VMT in the test group and more NMT in the control group. Regarding the intragroup comparison, the use of the membrane coverage presented significant outcomes in both groups. Therefore, in this experimental model, PRF did not affect the levels of bone formation when a membrane coverage technique was used. However, higher levels of bone formation were observed in the test group when membrane coverage was not used.

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Socket Preservation Using a (Dense) PTFE Barrier with or without Xenograft Material: A Randomized Clinical Trial

Materials ◽

10.3390/ma12182902 ◽

2019 ◽

Vol 12 (18) ◽

pp. 2902 ◽

Cited By ~ 1

Author(s):

Márcio de Carvalho Formiga ◽

Ulisses Ribeiro Campos Dayube ◽

Cristiane Kern Chiapetti ◽

Daniela de Rossi Figueiredo ◽

Jamil Awad Shibli

Keyword(s):

Alveolar Bone ◽

Blood Clot ◽

Test Group ◽

Control Group ◽

Socket Preservation ◽

Height Control ◽

Ptfe Membrane ◽

Implant Placement ◽

Bone Width ◽

Simple Alternative

When alveolar preservation procedures are not performed after tooth extraction, aesthetic and functional impairment could occur. Guided bone regeneration using polytetrafluoroethylene (PTFE) membranes has proven to be a simple alternative treatment that results in good maintenance of the alveolar bone for mediate/late implant placement. Therefore, this study compared the effect of alveolar preservation with the use of dense PTFE membranes, with and without xenograft material by Computerized tomography-based body composition (CTBC) analysis, after four months of the socket preservation procedure. A total of 29 teeth indicated for extraction. In the test group, the sockets were filled with bone graft biomaterial and subsequently coated with a dense PTFE membrane. In the control group, the sockets were filled with the blood clots and subsequently coated with a dense PTFE membrane. The results we found on the changes of the bone width and height after the procedures were: buccal plate: control group 0.46 mm, test group 0.91 mm; alveolar height: control group −0.41 mm, test group 0.35 mm; cervical third: control group −0.89 mm, test group −0.11 mm; middle third: control group −0.64, test group −0.50; and apical third: control group 0.09 mm, test group −0.14 mm. The use of a xenograft in conjunction with d-PTFE membranes proved to be superior to the use of the same membrane and blood clot only in regions of the crest, middle third, and alveolar height.

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Sinus Augmentation With Platelet-Rich Fibrin in Combination With Bovine Bone Graft Versus Bovine Bone Graft in Combination With Collagen Membrane

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-13-00129 ◽

2015 ◽

Vol 41 (5) ◽

pp. 586-595 ◽

Cited By ~ 15

Author(s):

Nilufer Bolukbasi ◽

Selim Ersanlı ◽

Nurullah Keklikoglu ◽

Cansu Basegmez ◽

Tayfun Ozdemir

Keyword(s):

Bone Graft ◽

Test Group ◽

Control Group ◽

Collagen Membrane ◽

Bovine Bone ◽

Graft Material ◽

Sinus Augmentation ◽

Platelet Rich Fibrin ◽

Bone Graft Material ◽

Sinus Floor

The purpose of this study was to compare the efficacy between the use of bovine bone graft material and platelet-rich fibrin (PRF) mixture (test group) and bovine bone graft material and collagen membrane combination (control group) in 2-stage maxillary sinus augmentation. According to specific inclusion/exclusion criteria, patients treated between 2008 and 2012 were selected. Panoramic radiographs were used for radiologic assessments. To evaluate the relationship between sinus-graft height and each implant, the bone level (BL) was divided by implant length (IL). To evaluate the change in the height of grafted sinus, the grafted sinus floor above the lowest part of the original sinus height (GSH) was divided by the original sinus height (OSH). Samples taken during implant surgery were used for histologic and histomorphometric analyses. Twenty-five patients, 32 augmentation surgeries, and 66 one-stage implants were included in the study. No implant loss or complication was observed in either group. There were no statistical differences according to new bone formation (P = .61) and biomaterial remnant (P = .87). During the evaluation period, the test group showed statistically less change in the BL/IL ratio (P = .022). The difference of GSH/OSH ratio was found to be insignificant between groups (P = .093). It was observed that the grafted sinus covering the implant apex and sinus floor was above the original sinus height in both groups. It may be concluded from this study that both combinations can be successfully used for sinus augmentation. Further studies evaluating different graft materials and PRF combinations in the early phases of healing would be beneficial.

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Effect of systemic antibiotics on the outcomes of regenerative periodontal surgery in intrabony defects: a randomized, controlled, clinical study

Clinical Oral Investigations ◽

10.1007/s00784-020-03616-7 ◽

2020 ◽

Author(s):

Małgorzata Pietruska ◽

Ewa Dolińska ◽

Robert Milewski ◽

Anton Sculean

Keyword(s):

Test Group ◽

Gingival Recession ◽

Control Group ◽

Collagen Membrane ◽

Antibiotic Administration ◽

Bovine Bone ◽

Intrabony Defects ◽

Periodontal Surgery ◽

Entire Study ◽

Systemic Antibiotics

Abstract Objectives To assess the potential influence of systemic antibiotic administration on the healing of periodontal intrabony defects treated with deproteinized bovine bone mineral (DBBM) and collagen membrane. Materials and methods Forty-one intrabony defects were treated by means of DBBM and collagen membrane (GTR). Postoperatively, the patients received either systemic antibiotics (i.e., 1 g of amoxicillin, twice daily for 7 days) (test) or no antibiotics (control). Clinical attachment level (CAL), probing depth (PD), and gingival recession (GR) were measured at baseline and at 1 year following regenerative surgery. The depth of the intrabony component (INTRA DD) and its width (INTRA DW) were measured during surgery and after 1 year at reentry. The depth (RxD) and width (RxW) of the intrabony defects were evaluated radiographically at baseline and at 1 year. Results No adverse events were observed in any of the two groups throughout the entire study period. In the test group, mean CAL changed from 8.7 ± 1.4 mm at baseline to 5.0 ± 1.7 mm at 1 year (p < 0.0001), while PD decreased from 7.8 ± 1.5 mm at baseline to 4.0 ± 0.9 mm at 1 year (p < 0.0001). In the control group, mean CAL changed from 8.6 ± 1.9 mm to 5.9 ± 1.6 mm (p < 0.001) and mean PD improved from 7.4 ± 1.3 mm to 4.1 ± 1.3 mm (p < 0.001). Mean CAL gain measured 3.6 ± 1.6 mm in the test and 2.7 ± 1.6 mm in the control group, respectively. Defect fill (i.e., INTRA DD gain) at re-entry measured 3.7 ± 1.8 mm in the test and 2.7 ± 2.1 mm in the control group. A CAL gain of ≥ 3 mm was measured in 76% of the defects in the test group and in 40% of the defects in the control group, respectively. In both groups, all evaluated clinical and radiographic parameters improved statistically significantly compared with baseline, but no statistically significant differences were found between the two groups. Conclusions Within their limits, the present study has failed to show any substantial added clinical benefits following the postoperative administration of amoxicillin in conjunction with regenerative periodontal surgery using DBBM and GTR. Clinical relevance The post-surgically administration of systemic antibiotics does not seem to be necessary following regenerative periodontal surgery.

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Use of Membrane with or without Bio-Oss For Extraction Socket Preservation: A Case Series

Research Society and Development ◽

10.33448/rsd-v9i12.11130 ◽

2020 ◽

Vol 9 (12) ◽

pp. e41991211130

Author(s):

Angelita Piovezana ◽

Danielle Gregorio ◽

Daiane Andrian de Oliveira Paludeto ◽

Letícia de Cássia Demeu Etore ◽

Marcos Heidy Guskuma ◽

...

Keyword(s):

Bone Repair ◽

Alveolar Bone ◽

Blood Clot ◽

Case Series ◽

Test Group ◽

Collagen Membrane ◽

Bovine Bone ◽

Significance Level ◽

Socket Preservation ◽

Computed Tomography Images

The purpose of this case report / case series was to analyze the pattern of alveolar bone repair after extraction with the use of a collagen membrane associated or not with lyophilized bovine bone (Bio-Oss). The sample consisted of six adult patients, aged from 40 to 60 years, with indication of extraction of the upper incisors and subsequent rehabilitation with dental implants, three participants were randomly distributed in the following groups: Test - Bio-Oss associated with collagen membrane absorbable; Control - blood clot associated with an absorbable collagen membrane. Alveoli with significant bone loss, probe greater than 4 mm, uncontrolled systemic changes and smoker were excluded. The less traumatic extractions, followed by anti-inflammatory and antibiotic protocol. Computed tomography images from the preoperative time (T0) and after 4 months (T1) were used to measure bone filling. The data were compared using the T test, considering a 5% significance level. Less resorption was observed in the test group (p = 0.002). The technique of preserving the alveolar ridge with Bio-Oss and absorbable membrane was satisfactory to prevent changes in the contour of the tissue.

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Histomorphometric Evaluation of Socket Preservation Using Autogenous Tooth Biomaterial and BM-MSC in Dogs

Scanning ◽

10.1155/2021/6676149 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Jin-Hyun Kim ◽

Puneet Wadhwa ◽

HongXin Cai ◽

Dong-Hyung Kim ◽

Bing Cheng Zhao ◽

...

Keyword(s):

Bone Graft ◽

Alveolar Bone ◽

Control Group ◽

Ridge Preservation ◽

Graft Material ◽

Socket Preservation ◽

Bone Graft Material ◽

Premolar Extraction ◽

Bone Width ◽

Significant Difference

This study is aimed at assessing the dimensional alterations occurring in the alveolar bone after premolar extraction in dogs with histomorphometric and histological analysis. After atraumatic premolar extraction, tooth-derived bone graft material was grafted in the extraction socket of the premolar region in the lower jaws of six dogs in two experimental groups. In the second experimental group, BM-MSCs were added together with the graft. The control was left untreated on the opposite side. After twelve weeks, all six animals were sacrificed. Differences in alveolar bone height crests lingually and buccally, and alveolar bone width at 1, 3, and 5 mm infracrestally, were examined. Histologic study revealed osteoconductive properties of tooth biomaterial. A statistically significant difference was detected between the test and control groups. In the test groups, a reduced loss of vertical and horizontal alveolar bone dimensions compared with the control group was observed. Tooth bone graft material may be considered useful for alveolar ridge preservation after tooth extraction, as it could limit the natural bone resorption process.

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Histological Comparison of New Bone Formation Using Amnion Membrane Graft Versus Resorbable Collagen Membrane: An Animal Study

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-16-00120 ◽

2018 ◽

Vol 44 (5) ◽

pp. 335-340 ◽

Cited By ~ 4

Author(s):

Soheil Koushaei ◽

Mohammad Hassan Samandari ◽

Sayed Mohammad Razavi ◽

Ahad Khoshzaban ◽

Shahriar Adibi ◽

...

Keyword(s):

Bone Formation ◽

Amniotic Membrane ◽

Clinical Impact ◽

Control Group ◽

Collagen Membrane ◽

New Bone Formation ◽

Histomorphometric Study ◽

Collagen Group ◽

Group 12 ◽

Amnion Membrane

The purpose of this article was to evaluate the bone induction effects of an amnion membrane–protected graft compared with a collagen membrane–protected graft in the repair of tibial bony defects in dogs. This study was performed using the tibial bone of dogs. After the removal of periosteum, similar holes were made with a 16-mm trephine drill (38 holes in total). For the study group, 10 holes were covered by absorbable collagen and 16 holes by amniotic membrane. In the control group, 12 holes were made and covered by the overlying soft tissue. Tibial bones were exposed after 6 and 12 weeks, and the samples were harvested and histologically processed. New bone formation was evaluated by histomorphometric study. Four Iranian mixed dogs older than 1.5 years were included in this study. The new bone formation was less in the control group when compared with the collagen group (P = .863). The collagen group showed less bone formation than the amnion group (P = .194), but this difference was not significant. However, bone formation in the amnion group was significantly more than in the control group (P = .050). Using the amniotic membrane appears to accelerate bone formation in guided bone regeneration. However, further studies should investigate its clinical impact on bone healing.

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Biphasic Calcium Phosphate Sphere Graft Combined with a Double-Layer Non-Crosslinked Collagen Membrane Technique for Ridge Preservation: A Randomized Controlled Animal Study

Materials ◽

10.3390/ma13010018 ◽

2019 ◽

Vol 13 (1) ◽

pp. 18 ◽

Cited By ~ 1

Author(s):

Jungwon Lee ◽

Young-Jun Lim ◽

Bongju Kim ◽

Ki-Tae Koo ◽

Yong-Moo Lee

Keyword(s):

Bone Graft ◽

Double Layer ◽

Single Layer ◽

Test Group ◽

Control Group ◽

Collagen Membrane ◽

Ridge Preservation ◽

Graft Material ◽

Membrane Technique ◽

Layer Membrane

The purpose of this study was to compare the histologic and radiologic differences between single- and double-layer collagen membrane techniques in flapless ridge preservation. The mandibular fourth premolar and first molar of four beagle dogs were used in the experiment. Mesial roots of the teeth were extracted and root canal treatment was performed at the distal roots. Ridge preservation was performed at the extraction sites using synthetic bone graft material. A single layer (control group) or double layer (test group) of non-crosslinked collagen membrane was applied following bone graft application. Three months later, the animals were sacrificed and micro-computed tomography (micro-CT) and histomorphometric analyses were conducted. Nonparametric Mann–Whitney test was performed to compare between the control and test groups. The vertical difference between buccal and lingual crests of control and test groups was 1.28 ± 0.41 and 0.53 ± 0.37 mm, respectively (p = 0.026). The mineralized bone area in control and test groups was 31.48% ± 7.41% and 42.25% ± 9.73%, respectively (p = 0.041). Within the limit of this study, ridge preservation using the double-layer membrane technique showed a reduced buccal bone resorption and improved new-bone formation in the ridge compared to that using the single-layer membrane technique.

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Comparison of Different Bone Filling Materials and Resorbable Membranes by Means of Micro-Tomography. A Preliminary Study in Rabbits

Materials ◽

10.3390/ma12081197 ◽

2019 ◽

Vol 12 (8) ◽

pp. 1197 ◽

Cited By ~ 2

Author(s):

Enrique Fernández-Bodereau ◽

Guillermo Dedossi ◽

Victor Ortega Asencio ◽

Manuel Fernández-Domínguez ◽

Sérgio Alexandre Gehrke ◽

...

Keyword(s):

Tissue Bank ◽

Fascia Lata ◽

Collagen Membrane ◽

Bovine Bone ◽

Filling Materials ◽

Bovine Collagen ◽

Barrier Membrane ◽

Group A ◽

Bone Filling ◽

Deproteinized Bovine Bone

The purpose of this work was to evaluate the behavior of different membranes and bone filling materials used to fill critical defects in rabbit calvaria. Four defects were prepared in the cranial calvaria of female rabbits. They were randomly divided into three subgroups according to the type of barrier membrane to be used. Four animals carried cross-linked bovine collagen membranes (Mem-Lok, Bio-Horizons, Birmingham, AL, USA)), four human fascia lata membranes (Tissue, Inbiomed SA, Córdoba, Argentina) and four human chorioamniotic membranes (Tissue. Inbiomed SA, Córdoba, Argentina). The defects were filled with the deproteinized bovine bone particulate BioOss® (GeistlichPharma AG, Wolhusen, Switzerland), with particulate human hydroxyapatite MinerOss® (Bio-Horizons, Birmingham, AL, USA), with particulate dental material (Tissue Bank Foundation, Inbiomed S.A., Córdoba, Argentina), and the last one was left without the addition of filler material. In the first group of four specimens, a resorbable cross-linked bovine collagen membrane was placed over the skull and defects, without additional fixing. In the second group, a human fascia lata membrane was placed, without additional fixing. In the third group, a human chorioamniotic membrane was placed, without additional fixing. The animals were sacrificed at 4 and 8 weeks. The highest percentages of relative radiological density (average) were recorded considering the amnio-chorionic membranes (83.63%) followed by collagen (81.44%) and finally the fascia lata membranes (80.63%), but the differences were not statistically significant (p > 0.05). The sites grafted with a decellularized tooth (96.83%) and BioOss (88.42%), recorded the highest percentages of radiological density but did not differ significantly from each other (subset 2). The three membranes used did not show statistical differences between them, in any of the two time periods used. There were statistical differences between the filling materials evidencing the presence of a large quantity of calcified material in the defects treated with particulate tooth and deproteinized bovine bone and while smaller amounts of calcified material were registered in the case of defects treated with human hydroxyapatite and those that were not treated.

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