scholarly journals Use of Membrane with or without Bio-Oss For Extraction Socket Preservation: A Case Series

2020 ◽  
Vol 9 (12) ◽  
pp. e41991211130
Author(s):  
Angelita Piovezana ◽  
Danielle Gregorio ◽  
Daiane Andrian de Oliveira Paludeto ◽  
Letícia de Cássia Demeu Etore ◽  
Marcos Heidy Guskuma ◽  
...  
Keyword(s):  
Bone Repair ◽  
Alveolar Bone ◽  
Blood Clot ◽  
Case Series ◽  
Test Group ◽  
Collagen Membrane ◽  
Bovine Bone ◽  
Significance Level ◽  
Socket Preservation ◽  
Computed Tomography Images

The purpose of this case report / case series was to analyze the pattern of alveolar bone repair after extraction with the use of a collagen membrane associated or not with lyophilized bovine bone (Bio-Oss). The sample consisted of six adult patients, aged from 40 to 60 years, with indication of extraction of the upper incisors and subsequent rehabilitation with dental implants, three participants were randomly distributed in the following groups: Test - Bio-Oss associated with collagen membrane absorbable; Control - blood clot associated with an absorbable collagen membrane. Alveoli with significant bone loss, probe greater than 4 mm, uncontrolled systemic changes and smoker were excluded. The less traumatic extractions, followed by anti-inflammatory and antibiotic protocol. Computed tomography images from the preoperative time (T0) and after 4 months (T1) were used to measure bone filling. The data were compared using the T test, considering a 5% significance level. Less resorption was observed in the test group (p = 0.002). The technique of preserving the alveolar ridge with Bio-Oss and absorbable membrane was satisfactory to prevent changes in the contour of the tissue.

scholarly journals Socket Preservation Using a (Dense) PTFE Barrier with or without Xenograft Material: A Randomized Clinical Trial

Materials ◽  
2019 ◽  
Vol 12 (18) ◽  
pp. 2902 ◽  
Author(s):  
Márcio de Carvalho Formiga ◽  
Ulisses Ribeiro Campos Dayube ◽  
Cristiane Kern Chiapetti ◽  
Daniela de Rossi Figueiredo ◽  
Jamil Awad Shibli
Keyword(s):  
Alveolar Bone ◽  
Blood Clot ◽  
Test Group ◽  
Control Group ◽  
Socket Preservation ◽  
Height Control ◽  
Ptfe Membrane ◽  
Implant Placement ◽  
Bone Width ◽  
Simple Alternative

When alveolar preservation procedures are not performed after tooth extraction, aesthetic and functional impairment could occur. Guided bone regeneration using polytetrafluoroethylene (PTFE) membranes has proven to be a simple alternative treatment that results in good maintenance of the alveolar bone for mediate/late implant placement. Therefore, this study compared the effect of alveolar preservation with the use of dense PTFE membranes, with and without xenograft material by Computerized tomography-based body composition (CTBC) analysis, after four months of the socket preservation procedure. A total of 29 teeth indicated for extraction. In the test group, the sockets were filled with bone graft biomaterial and subsequently coated with a dense PTFE membrane. In the control group, the sockets were filled with the blood clots and subsequently coated with a dense PTFE membrane. The results we found on the changes of the bone width and height after the procedures were: buccal plate: control group 0.46 mm, test group 0.91 mm; alveolar height: control group −0.41 mm, test group 0.35 mm; cervical third: control group −0.89 mm, test group −0.11 mm; middle third: control group −0.64, test group −0.50; and apical third: control group 0.09 mm, test group −0.14 mm. The use of a xenograft in conjunction with d-PTFE membranes proved to be superior to the use of the same membrane and blood clot only in regions of the crest, middle third, and alveolar height.

scholarly journals Functionalized Scaffold and Barrier Membrane with Anti-BMP-2 Monoclonal Antibodies for Alveolar Ridge Preservation in a Canine Model

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Seiko Min ◽  
Taewan Kim ◽  
Oksu Kim ◽  
Carames Goncalo ◽  
Tadahiko Utsunomiya ◽  
...  
Keyword(s):  
Bone Formation ◽  
Alveolar Bone ◽  
Test Group ◽  
Canine Model ◽  
Bone Morphogenetic Protein 2 ◽  
Control Group ◽  
Collagen Membrane ◽  
Bovine Bone ◽  
Barrier Membrane ◽  
Bone Width

Introduction. The aim of this study was to investigate the ability of anti-bone morphogenetic protein 2 monoclonal antibody (anti-BMP-2 mAb) to functionalize scaffolds to mediate bone regeneration in a canine model. Materials and Methods. The mandibular right premolar 4 (PM4) was extracted in eight beagle dogs and grafted with anti-BMP-2 mAb+anorganic bovine bone mineral with 10% collagen (ABBM-C) and porcine bilayer native collagen membrane (CM). The ABBM-C and CM were functionalized with either anti-BMP-2 mAb (test group) or an isotype matched control mAb (control group). Animals were euthanized at 12 weeks for radiographic, histologic, and histomorphometric analyses. Outcomes were compared between groups. Results. 3D imaging using cone beam computed tomography (CBCT) revealed that sites treated with ABBM-C and CM functionalized with anti-BMP-2 mAb exhibited significantly more remaining bone width near the alveolar crest, as well as buccal bone height, compared with control groups. Histologic and histomorphometric analyses demonstrated that in anti-BMP-2 mAb-treated sites, total tissue volume was significantly higher in the coronal part of the alveolar bone crest compared with control sites. In anti-BMP-2 mAb-treated sites, bone formation was observed under the barrier membrane. Conclusion. Functionalization of the ABBM-C scaffold and CM appeared to have led to bone formation within healing alveolar bone sockets.

scholarly journals The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism

2013 ◽  
Vol 65 (3) ◽  
pp. 715-721 ◽  
Author(s):  
R.L. Buchaim ◽  
J.C. Andreo ◽  
A. C. Rodrigues ◽  
D.V. Buchaim ◽  
D.V. Dias ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Repair ◽  
Blood Clot ◽  
Bone Matrix ◽  
Male Rats ◽  
Bovine Bone ◽  
Filling Material ◽  
Tissue Formation ◽  
Bone Trabeculae ◽  
Bone Tissue Formation

The objective of this study was to evaluate whether demineralized bovine bone (Gen-ox®) alters bone neoformation in rats submitted to alcoholism. Forty male rats were separated into two groups of 20 rats and distributed as follows: Group E1, which received 25% ethanol and a surgical cavity filled only by a blood clot, and Group E2, which received 25% ethanol and a surgical cavity filled with Gen-ox®. The animals were euthanized at 10, 20, 40 and 60 days after surgery and necropsy was performed. The histomorphological and histometric analyses of the area of connective tissue and bone neoformation showed that the reorganization of the bone marrow and full repair of the surgical cavity in Group E1 occurred more quickly than in Group E2. It was also noted that in the final period the animals in Group E2 showed areas of connective tissue and thick bone trabeculae around the particles of the implant. It can be concluded that the use of Gen-ox® delayed the process of bone repair in alcoholic rats, although it can be used as filling material because it shows osteoconductive activity, as evidenced by bone tissue formation around the graft particles.

scholarly journals Comparison between fractal analysis and radiopacity evaluation as a tool for studying repair of an osseous defect in an animal model using biomaterials

2019 ◽  
Vol 48 (7) ◽  
pp. 20180466
Author(s):  
Nathália R. Gomes ◽  
Juliano D.S. Albergaria ◽  
Jonathas A. de S. Henriques ◽  
Tânia M.P. Amaral ◽  
Evandro N. Abdo ◽  
...  
Keyword(s):  
Animal Model ◽  
Fractal Analysis ◽  
Bone Repair ◽  
Blood Clot ◽  
Bovine Bone ◽  
Final Time ◽  
Significant Difference ◽  
Final Time Point ◽  
Osseous Defect ◽  
Time Point

Objectives: To evaluate bone repair of an osseous defect in a rat animal model through fractal analysis and radiopacity analysis in radiographic images. Methods: 120 rats were subjected to extraction of their first molar and divided into four groups (n = 6/group) according to the material used for bone grafting: mineralized bovine bone, demineralized bovine bone (DBB), blood clot (BC - control) or Bio-Oss® (BO). The animals were sacrificed after 1, 7, 14, 21 and 49 days and subjected to radiographic evaluation. For fractal analysis (FA), a square regionof interest of 30 × 30 pixels was used, and radiopacity was measured as the mean gray scale (MGS) value for three points of 5 × 5 pixels in the apical, medial and coronal regions of the defect. Histomorphometric evaluation was realized as the gold standard for bone neo-formation and maturation of the new osseous matrix. Results: Histomorphometric evaluation suggested that DBB showed faster mineralized deposition and resulted in more mature bone at the final time point of evaluation. Mineralized bovine bone and Bio-Oss presented similar results. The mineralized groups did not show significant differences in bone maturation. The radiopacity analysis revealed a significant difference (p < 0.05) between the DBB and blood clot groups at the final time point. FA did not show any significant differences at the final time point. Conclusions: Mean gray scale seemed to be more effective for the quantification of bone repair than FA in the demineralized group in this animal model. Results for the mineralized groups did not reveal a significant difference, leading to the conclusion that both methods are effective.

scholarly journals Pool of bovine morphogenetic proteins and guided tissue regeneration in the treatment of intrabony periodontal defects: I- Clinical measurements

2004 ◽  
Vol 12 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Maria do Carmo Machado Guimarães ◽  
Euloir Passanezi ◽  
Adriana Campos Passanezi Sant'Ana ◽  
Sebastião Luiz Aguiar Greghi
Keyword(s):  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Test Group ◽  
Control Group ◽  
Collagen Membrane ◽  
Bovine Bone ◽  
Intrabony Defects ◽  
Bovine Collagen ◽  
Barrier Membrane ◽  
Clinical Measurements

The purpose of this study was to evaluate the effect of the pool of bovine BMPs on the treatment of intrabony defects. The sample comprised 15 patients aged 26 to 57 years old presenting with 10 pairs of lesions of 2 or 3 walls or 2-3 walls ³5mm, located in the same type teeth (premolar or molar) and same jaw. The test defects were treated with combination of a pool of bovine bone morphogenetic and resorbable hydroxyapatite carrier (BMPs- HA), bovine demineralized bone matrix (MB) and coverage by a bovine collagen barrier membrane. The control defects were treated with MB-HA and covered by a bovine collagen membrane. The clinical measurements at six months after therapy in the test group revealed a reduction in the mean probing pocket depth (PPD) of 1.63 ± 1.41mm (B) and 1.93 ± 0.96mm (L) and a mean change in the clinical attachment level (CAL) of 1.60 ± 1.16mm (B) and 1.46 ± 0.97mm (L). The control group showed a mean reduction of PPD of 1.93 ± 1.34mm (B) and 2.0 ± 1.51mm (L) and a mean change of CAL of 1.03 ± 1.24mm (B) and 1.30 ± 1.14 mm (L). The analysis of variance (ANOVA) demonstrated that the changes in the clinical parameters were statistically significant (p<0.05). There were no significant differences between the test and controls subjects (Student's test, p<0.005). These findings suggest that the use of a pool of bovine BMPs do not provide added effects to GTR in the treatment of intrabony defects.

scholarly journals Randomized Controlled Clinical Trial of Nanostructured Carbonated Hydroxyapatite for Alveolar Bone Repair

Materials ◽  
2019 ◽  
Vol 12 (22) ◽  
pp. 3645 ◽  
Author(s):  
Rodrigo F. B. Resende ◽  
Suelen C. Sartoretto ◽  
Marcelo J. Uzeda ◽  
Adriana T. N. N. Alves ◽  
José A. Calasans-Maia ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Bone Repair ◽  
Alveolar Bone ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Randomized Controlled Clinical Trial ◽  
Double Blind ◽  
Socket Preservation ◽  
Carbonated Hydroxyapatite ◽  
Randomized Controlled

The properties of the biodegradation of bone substitutes in the dental socket after extraction is one of the goals of regenerative medicine. This double-blind, randomized, controlled clinical trial aimed to compare the effects of a new bioabsorbable nanostructured carbonated hydroxyapatite (CHA) with a commercially available bovine xenograft (Bio-Oss®) and clot (control group) in alveolar preservation. Thirty participants who required tooth extraction and implant placement were enrolled in this study. After 90 days, a sample of the grafted area was obtained for histological and histomorphometric evaluation and an implant was installed at the site. All surgical procedures were successfully carried out without complications and none of the patients were excluded. The samples revealed a statistically significant increase of new bone formation (NFB) in the CHA group compared with Bio-Oss® after 90 days from surgery (p < 0.05). However, the clot group presented no differences of NFB compared to CHA and Bio-Oss®. The CHA group presented less amount of reminiscent biomaterial compared to Bio-Oss®. Both biomaterials were considered osteoconductors, easy to handle, biocompatible, and suitable for alveolar filling. Nanostructured carbonated hydroxyapatite spheres promoted a higher biodegradation rate and is a promising biomaterial for alveolar socket preservation before implant treatment.

Outcome of periodontal–orthodontic treatment in subjects with periodontal disease. Part II: a CBCT study of alveolar bone level changes

2019 ◽  
Vol 41 (6) ◽  
pp. 565-574 ◽  
Author(s):  
Eglė Zasčiurinskienė ◽  
Henrik Lund ◽  
Rune Lindsten ◽  
Henrik Jansson ◽  
Krister Bjerklin
Keyword(s):  
Periodontal Disease ◽  
Orthodontic Treatment ◽  
Alveolar Bone ◽  
Test Group ◽  
Periodontal Treatment ◽  
Control Group ◽  
Computed Tomography Images ◽  
Significant Difference ◽  
Bone Level ◽  
Maxillary Incisors

Abstract Aim To examine alveolar bone level (ABL) changes before (T1) and after (T2) orthodontic treatment (OT) in subjects with periodontal disease. Methods The study included 50 subjects with periodontal disease. All patients received subgingival debridement following baseline examination. Control group patients received final periodontal treatment before the start of OT. For the test group patients final periodontal treatment was performed simultaneous to OT. OT was performed with a straight-wire appliance. Micro-implants or temporary crowns on implants were used for posterior anchorage when needed. ABL measurements of 3821 tooth surfaces were performed on cone beam computed tomography images. Results No difference was observed between mean ABL at T1 and T2. ABL remained unchanged on 69 per cent of surfaces. A mean of 15.6 (SD 7.4) per cent of surfaces experienced ABL gain, and a mean of 15.1 (SD 7.5) per cent was found with ABL loss. Small significant median ABL difference was observed on mesial and distal surfaces (P < 0.001). A significant difference was found between median ABL changes on mesial/distal in comparison to buccal/lingual surfaces (P < 0.01). Significantly more buccal (17.9 %) and lingual (18.5 %) surfaces experienced ABL loss when compared with mesial (11.3 %) and distal (12.0 %) surfaces (P < 0.001). Significant difference was found in the median ABL change of intruded (0.5 (IQR 0.94) mm) and non-intruded (−0.4 (IQR 1.07) mm) maxillary incisors (P = 0.04). Significant median ABL gain was found on the lingual surface of maxillary incisors, which were retroclined more than 8.6 degree and intruded more than 1.6 mm. Conclusions ABL changes after periodontal–orthodontic treatment in patients with periodontal disease were small. ABL gain was more observed on mesial and distal surfaces and ABL loss on buccal and lingual surfaces. Larger orthodontic movements of maxillary incisors influenced ABL gain.

scholarly journals Histological Comparison between Biphasic Calcium Phosphate and Deproteinized Bovine Bone on Critical-Size Bone Defects

2021 ◽  
Vol 32 (1) ◽  
pp. 26-33
Author(s):  
Marina Araujo Brito ◽  
Leomar Emanuel Almeida Mecca ◽  
Thais dos Santos Sedoski ◽  
Tayline Mroczek ◽  
Marcela Claudino ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Bone Repair ◽  
Critical Size ◽  
Blood Clot ◽  
Bone Defects ◽  
Bovine Bone ◽  
Tissue Area ◽  
Deproteinized Bovine Bone

Abstract The limited options for bone repair have led to an extensive research of the field and the development of alloplastic and xenogeneic grafts. The purpose of this study was to evaluate bone repair with two bone substitutes: deproteinized bovine bone (DBB) and biphasic calcium phosphate ceramic (BCP) in critical-size defect. A total of 8-mm defects were made in the parietal bones of rabbits (n=12). The animals were divided into three experimental groups: sham (defect filled with a blood clot), DBB (defect filled with DBB), and BCP (defect filled with BCP). After the experimental periods of 15 and 45 days, the animals were euthanized and submitted to histomorphometric analysis. The total defect area, mineralized tissue area, biomaterial area, and soft tissue area were evaluated. A greater amount of immature bone tissue and biomaterial particles were observed in the BCP group compared to DBB and sham at 45 days (p<0.05). There was no difference in the qualitative pattern of bone deposition between DBB and BCP. However, the sham group did not show osteoid islands along with the defect, presenting a greater amount of collagen fibers as well in relation to the DBB and BCP groups. There was a greater number of inflammatory cells in the DBB at 45 days compared to BCP and sham groups. In conclusion, BCP and DBB are options for optimizing the use of bone grafts for maxillofacial rehabilitation. Bone defects treated with BCP showed greater deposition of bone tissue at 45 days.

scholarly journals The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria

2017 ◽  
Vol 16 ◽  
pp. 1-11
Author(s):  
Luiz Gustavo Klug ◽  
Carmen Lucia Mueller Storer ◽  
Aline Monise Sebastiani ◽  
Bruna Lorena Bsrbaresco ◽  
Alan Fernando Giovanini ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Bone Repair ◽  
Critical Size ◽  
Blood Clot ◽  
Control Group ◽  
Significance Level ◽  
Tissue Graft ◽  
Critical Size Defects ◽  
The Mean ◽  
Mean Concentration

The proteins Wnts are considered a key regulator of the early development of the skeleton. Aim: The aim of this study was to evaluate the presence of the protein Wnt10b as a marker of bone repair in critical size defects surgically created in the calvaria of rabbits treated with fragmented autogenous adipose tissue graft. Methods: A total of 28 rabbits were divided into two groups: the Control group (C) and Adipose Tissue Graft group (ATG). A CSD measuring 15 mm in diameter was created in the calvaria of each animal. In rabbits of the C group, the defect was filled only with blood clot, and in ATG group, the defect was filled with fragmented adipose tissue graft. The two groups were divided into two subgroups (n = 7) for euthanasia 15 and 40 days after surgery. Histological and immunohistochemically analyses were performed to evaluate the neoformed bone and the presence/concentration of Wnt10b protein. The Kruskal-Wallis test was performed to compare the means and standard deviations of the number of Wnt10b + cells/mm2 in both groups in each postoperative period. It was assumed a significance level of 5%. Results: After 40 days, the mean concentration of the protein Wnt10b in ATG group was 26.26 (+-6.97) significant higher (p<0,001) than the mean in C group that was 305 (37.41). Conclusion: The protein Wnt10b would play a crucial role in the signaling of bone formation in bone defects treated with fragmented autogenous adipose tissue graft.

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