scholarly journals Effects of Natural Products on Bacterial Communication and Network-Quorum Sensing

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Min Yang ◽  
Fanying Meng ◽  
Wen Gu ◽  
Fengjiao Li ◽  
Yating Tao ◽  
...  
Keyword(s):  
Natural Products ◽  
Quorum Sensing ◽  
Virulence Factor ◽  
Biofilm Formation ◽  
Bacterial Flora ◽  
External Environment ◽  
Cell Communication ◽  
Beneficial Bacteria ◽  
Bacterial Communication ◽  
Virulence Factor Production

Quorum sensing (QS) has emerged as a research hotspot in microbiology and medicine. QS is a regulatory cell communication system used by bacterial flora to signal to the external environment. QS influences bacterial growth, proliferation, biofilm formation, virulence factor production, antibiotic synthesis, and environmental adaptation. Through the QS system, natural products can regulate the growth of harmful bacteria and enhance the growth of beneficial bacteria, thereby improving human health. Herein, we review advances in the discovery of natural products that regulate bacterial QS systems.

In vitro and in vivo efficacy of rosmarinic acid on quorum sensing mediated biofilm formation and virulence factor production in Aeromonas hydrophila

Biofouling ◽  
2016 ◽  
Vol 32 (10) ◽  
pp. 1171-1183 ◽  
Author(s):  
Kannan Rama Devi ◽  
Ramanathan Srinivasan ◽  
Arunachalam Kannappan ◽  
Sivasubramanian Santhakumari ◽  
Murugan Bhuvaneswari ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Virulence Factor ◽  
Aeromonas Hydrophila ◽  
Rosmarinic Acid ◽  
Biofilm Formation ◽  
In Vivo Efficacy ◽  
Factor Production ◽  
Virulence Factor Production

scholarly journals The PqsE-RhlR Interaction Regulates RhlR DNA Binding to Control Virulence Factor Production in Pseudomonas aeruginosa

2022 ◽  
Author(s):  
Kayla A. Simanek ◽  
Isabelle R. Taylor ◽  
Erica K. Richael ◽  
Erica Lasek-Nesselquist ◽  
Bonnie L. Bassler ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Dna Binding ◽  
Quorum Sensing ◽  
Virulence Factor ◽  
Cell Communication ◽  
Communication Process ◽  
Collective Behaviors ◽  
Factor Production ◽  
A Cell ◽  
Virulence Factor Production

Bacteria use a cell-cell communication process called quorum sensing (QS) to orchestrate collective behaviors. QS relies on the group-wide detection of molecules called autoinducers (AI).

scholarly journals Quorum-Sensing Antagonistic Activities of Azithromycin in Pseudomonas aeruginosa PAO1: a Global Approach

2006 ◽  
Vol 50 (5) ◽  
pp. 1680-1688 ◽  
Author(s):  
Yusuf Nalca ◽  
Lothar Jänsch ◽  
Florian Bredenbruch ◽  
Robert Geffers ◽  
Jan Buer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Virulence Factor ◽  
Biofilm Formation ◽  
Great Promise ◽  
Factor Production ◽  
The Impact ◽  
Virulence Factor Production

ABSTRACT The administration of macrolides such as azithromycin for chronic pulmonary infection of cystic fibrosis patients has been reported to be of benefit. Although the mechanisms of action remain obscure, anti-inflammatory effects as well as interference of the macrolide with Pseudomonas aeruginosa virulence factor production have been suggested to contribute to an improved clinical outcome. In this study we used a systematic approach and analyzed the impact of azithromycin on the global transcriptional pattern and the protein expression profile of P. aeruginosa PAO1 cultures versus those in untreated controls. The most remarkable result of this study is the finding that azithromycin exhibited extensive quorum-sensing antagonistic activities. In accordance with the inhibition of the quorum-sensing systems, virulence factor production was diminished and the oxidative stress response was impaired, whereas the type III secretion system was strongly induced. Moreover, P. aeruginosa motility was reduced, which probably accounts for the previously observed impaired biofilm formation capabilities of azithromycin-treated cultures. The interference of azithromycin with quorum-sensing-dependent virulence factor production, biofilm formation, and oxidative stress resistance in P. aeruginosa holds great promise for macrolide therapy in cystic fibrosis. Clearly quorum-sensing antagonist macrolides should be paid more attention in the management of chronic P. aeruginosa infections, and as quorum-sensing antagonists, macrolides might gain vital importance for more general application against chronic infections.

scholarly journals Quorum sensing-regulated functions of Serratia marcescens are reduced by eugenol

2020 ◽  
Author(s):  
Zahra Fekrirad ◽  
Basira Gattali ◽  
Nasim Kashef
Keyword(s):  
Quorum Sensing ◽  
Serratia Marcescens ◽  
Virulence Factor ◽  
Biofilm Formation ◽  
Protease Production ◽  
Resistant Bacteria ◽  
Pigment Formation ◽  
Factor Production ◽  
Regulated Functions ◽  
Virulence Factor Production

Background and Objectives: Serratia marcescens has emerged as a nosocomial pathogen responsible for human infections, where antibiotic resistance further complicates the treatments. In S. marcescens, biofilm formation and virulence factor production are controlled via quorum sensing (QS) system. QS is a signaling system that enables gene regulation to control diverse physiological functions in bacteria. Essential oils have shown to be potential in diminishing the pathogenicity and virulence of drug-resistant bacteria. This study was performed to determine whether eugenol would affect QS system, biofilm formation and virulence factor production of S. marcescens. Materials and Methods: Biofilm formation, extracellular virulence factor production (hemolysin and protease), swarming motility and pigment formation of S. marcescens ATCC 13880 and S. marcescens Sm2 were assessed after eugenol exposure at 1.25 and 2.5 µg/ml concentrations. The expression of genes involved in motility (flhD), attachment (fimC), biofilm formation (bsmB, bsmA), and QS regulatory (swrR) were also evaluated. Results: Eugenol treatment at 1.25 and 2.5 µg/ml concentrations caused a significant reduction in biofilm formation. The pigment, hemolysin and protease production of two studied S. marcescens strains, also reduced significantly by eugenol treatments (p<0.05). The bsmA, bsmB, flhD and fimC genes were down-regulated after eugenol treatment. The swrR gene expression was also reduced significantly by eugenol in both S. marcescens strains (p<0.05). Conclusion: Eugenol inhibited quorum sensing-regulated functions of two studied S. marcescens strains.

scholarly journals A Novel Use of Allopurinol as A Quorum-Sensing Inhibitor in Pseudomonas aeruginosa

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1385
Author(s):  
Ahmed Al Saqr ◽  
Mohammed F. Aldawsari ◽  
El-Sayed Khafagy ◽  
Moataz A. Shaldam ◽  
Wael A. H. Hegazy ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Virulence Factors ◽  
Virulence Factor ◽  
Cell Communication ◽  
Factor Production ◽  
In Silico Study ◽  
Liver And Kidney ◽  
Healthcare Associated ◽  
Virulence Factor Production

Pseudomonas aeruginosa can cause a variety of healthcare-associated infections by its arsenal of virulence factors. Virulence factor production is largely controlled by the cell-to-cell communication system termed quorum sensing (QS). Targeting QS may be a good approach to inhibit the production of virulence factors and attenuate pathogenicity without exerting selective stress on bacterial growth. This will greatly reduce the emergence of resistant mutants. In this work, we investigated the anti-virulence and anti-QS activities of the FDA-approved drug allopurinol against the P. aeruginosa PAO1 strain. Allopurinol at 200 µg/mL (1/10 MIC) significantly decreased the production of the QS-controlled Chromobacterium violaceum CV026 violet pigment violacein and other P. aeruginosa QS-controlled virulence factors phenotypically. Furthermore, allopurinol reduced the infiltration of P. aeruginosa and leucocytes and diminished the congestion in the liver and kidney tissues of infected mice. In silico study showed that allopurinol could compete with the autoinducers on binding to the receptors LasR and RhlR by hydrogen bonding. On the molecular level, qRT-PCR proved that allopurinol showed a significant downregulating effect on all tested QS-encoding genes that regulate virulence factor production. In summary, allopurinol is a promising QS inhibitor that may be useful in the future treatment of P. aeruginosa infection.

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