Antioxidant Activity Derived from Marine Green-Lipped Mussel Perna canaliculus Extracts in Mice

This study investigates the antioxidant activities of lipid, protein, and carbohydrate extracts from the marine mollusk Perna canaliculus. Lipids were extracted using acetone, which was followed by protein extraction using the broad-spectrum enzyme Alcalase and then carbohydrate extraction using cetylpyridinium chloride. Eighty white BALB/c mice were divided into eight groups according to the administered extracts. Groups 1 and 5 were the control and toxin control groups, respectively. Groups 2, 3, and 4 were administered lipid, protein, and carbohydrate extracts, respectively. The other groups were administered P. canaliculus extracts as well as gentamicin and acetaminophen, known as ethanolic extracts, derived from Nerium oleander to induce oxidation stress. All groups showed significant improvements in body weight ( p < 0.05 ). The lipid extract group showed a significant decrease in low-density lipoprotein cholesterol ( p < 0.05 ) and a significant increase in high-density lipoprotein cholesterol ( p < 0.05 ). After the toxin injection, all groups treated with P. canaliculus extracts showed increased antioxidant effects on hepatocytes ( p < 0.05 ). The lipid extracts induced antioxidant effects to protect the kidney by increasing lipid peroxidation ( p < 0.05 ) and catalase activities ( p < 0.05 ). Also, protein extracts showed antioxidant effects by increasing glutathione and catalase levels significantly ( p < 0.005 ). In conclusion, P. canaliculus extracts, especially lipids and proteins, have potent antioxidant activities that protect vital organs from oxidation stress.

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ANTIHYPERGLYCEMIC, ANTIOXIDANT, AND ANTIHYPERLIPIDEMIC ACTIVITIES OF EXTRACT FROM COCCINIA GRANDIS (L.) VOIGT. LEAVES BY METHANOL ON ALLOXAN INDUCED HYPERGLYCEMIC MICE

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/16/2/13443 ◽

2018 ◽

Vol 16 (2) ◽

pp. 311-318

Author(s):

Dai Thi Xuan Trang ◽

Vo Chi Linh ◽

Nguyen Thi Ai Lan

Keyword(s):

Body Weight ◽

Antioxidant Activities ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Lipoprotein Cholesterol ◽

Glucose Levels ◽

Coccinia Grandis ◽

Blood Glucose Levels ◽

Alloxan Monohydrate ◽

Antioxidant Effects

The aim of this study was to investigate antihyperglycemic, antihyperlipidemic and antioxidant activities of the extract of Coccinia grandis leaves (CGL) by methanol in alloxan induced diabetic mice. Hyperglycemic was induced in mice by administration of alloxan monohydrate (135 mg/kg, ip.). CGL was orally given to the normal mice (100 and 1000 mg/kg body weight) to determine the toxicity of CGL. Hyperglycemic mice were treated with the extract of CGL (100 mg/kg body weight/ twice a day). Glucophage, diabetic commercially available drug, was used as a possitive control with dose of 108 mg/kg body weight/twice a day. Mice were administered with both glucophage and CGL extract orally during 21 days. On the 21st day of the experiment, mice blood, liver and brain samples were collected to test biochemical parameters and antioxidant levels. CGL did not show toxicity and death up to dose 1000 mg/kg in mice. Administration of CGL 100 mg/kg significantly (p<0.05) reduced blood glucose levels in alloxan induced hyperglycemic mice. Besides, several serum lipid values including total cholecterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) reduced while high density lipoprotein cholesterol (HDL-C) increased. Malondialdehyde (MDA) in a group of untreated hyperglycemic mice was statistically higher than that of nomal mice. The MDA levels of livers and brains in the CGL treated hyperglycemic mice were lower than that of hyperglycemic control. The effects of hypoglycemic, antihyperlipidemic and antioxidant of CGL were higher than that of glucophage. The total polyphenols and flavonoids contents of the leaf extract were 607,41 + 14,44 mg gallic acid or quercetin equivalents/g and 111,72 + 1,94 mg equivalents/g, respectively. In conclusion, the antihyperglycemic, antihyperlipidemic and antioxidant effects of the CGL suggest a potential therapeutictreatment for hyperglycemic conditions.

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Effect of Antioxidant Mineral Elements Supplementation in the Treatment of Hypertension in Albino Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2012/134723 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

S. A. Muhammad ◽

L. S. Bilbis ◽

Y. Saidu ◽

Y. Adamu

Keyword(s):

Oxidative Stress ◽

Antioxidant Activities ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Lipoprotein Cholesterol ◽

Very Low Density Lipoprotein ◽

Low Density ◽

Low Density Lipoprotein Cholesterol ◽

Treatment Of Hypertension ◽

Total Antioxidant

Oxidative stress has been implicated in various pathologies, including hypertension, atherosclerosis, diabetes, and chronic renal disease. The current work was designed with the aim of investigating the potentials of antioxidants copper, manganese, and zinc in the treatment of hypertension in Wistar rats. The rats were fed 8% NaCl diet for 5 weeks and treatment with supplements in the presence of the challenging agent for additional 4 weeks. The supplementation significantly decreased the blood pressure as compared with hypertensive control. The result also indicated significant decreased in the levels of total cholesterol, triglyceride, low-density lipoprotein cholesterol and very low-density lipoprotein cholesterol, malondialdehyde, insulin and increase in the high-density lipoprotein cholesterol, total antioxidant activities, and nitric oxide of the supplemented groups relative to the hypertensive control. The average percentage protection against atherogenesis indicated 47.13 ± 9.60% for all the supplemented groups. The mean arterial blood pressure showed significant positive correlation with glucose, total cholesterol, triglyceride, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, atherogenic index, insulin resistance and malondialdehyde while high density lipoprotein-cholesterol and total antioxidant activities showed negative correlation. The result therefore indicated strong relationship between oxidative stress and hypertension and underscores the role of antioxidant minerals in reducing oxidative stress, dyslipidemia, and insulin resistance associated with hypertension.

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Bioantioxidants: From chemistry to biology

Pure and Applied Chemistry ◽

10.1351/pac200577111887 ◽

2005 ◽

Vol 77 (11) ◽

pp. 1887-1903 ◽

Cited By ~ 20

Author(s):

Bo Zhou ◽

Zhong-Li Liu

Keyword(s):

Therapeutic Strategy ◽

Antioxidant Activities ◽

Biological Activities ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Green Tea Polyphenols ◽

Oxidative Damages ◽

Naturally Occurring ◽

Current Kinetic ◽

Antioxidant Effects

Bioantioxidants, or dietary antioxidants, are dietary substances in food that significantly decrease the adverse effects of reactive oxygen species (ROS) on normal physiological functions in humans. Inhibition of ROS-induced oxidative damages by supplementation of bioantioxidants, the so-called "antioxidant therapy", has become an attractive therapeutic strategy to reduce the risk of ROS-related diseases and has led to flourishing research in the past decade. However, many questions dealing with the correlation between chemical and biological activities, the bioavailability and "non-antioxidant" effects of bioantioxidants are still under debate. This article outlines our current kinetic and mechanistic studies on naturally occurring antioxidants, including vitamin E, green tea polyphenols (GOHs), and resveratrol, as well as their synthetic analogs in micelles, in red blood cells, in low-density lipoprotein (LDL) and in microsomes. The cytotoxicity and apoptosis-inducing activity of these antioxidants against cancer cells were also studied. It was found that there was significant correlation between the chemical and biological antioxidant activities, as well as between the antioxidant activity and the cytotoxic and apoptosis-inducing activities.

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Antioxidant potential and low-density lipoprotein cholesterol (LDL-c) uptake of the black seed and honey mixture on human hepatocellular carcinoma (HepG2) cells

Food Research ◽

10.26656/fr.2017.5(6).730 ◽

2021 ◽

Vol 5 (6) ◽

pp. 141-149

Author(s):

N.S. Mohd Isa ◽

J.S. Ng ◽

F. Tufail Ahmad ◽

M.N.I. Kassim ◽

Norhayati H. ◽

...

Keyword(s):

Hepg2 Cells ◽

Antioxidant Activities ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Lipoprotein Cholesterol ◽

Lipid Lowering ◽

Low Density ◽

Low Density Lipoprotein Cholesterol ◽

Black Seed ◽

Significant Difference

High lipid levels especially low-density-lipoprotein cholesterol (LDL-c) are associated with increased risk of cardiovascular (CVD) and coronary heart disease (CHD). Both black seed (Nigella sativa L.) and honey are well-known in the hypolipidemic potential and have CVD protective effects. In the present study, LDL-c uptake of the black seed and honey mixture was tested on HepG2 cells. Antioxidant activities of black seed and honey mixtures were determined through the 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) assay. The anticancer potential of black seed and honey mixtures in HepG2 cells was performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay. Black seed possessed the highest antioxidant activities with EC50 6.54 mg/mL as compared to honey with EC50 value 9.56 mg/mL while the black seed and honey mixture have EC50 between black seed and honey. From the results obtained, no synergistic effect was observed in the mixtures as the EC50 values were within the range of black seed and honey. Furthermore, no significant difference (p>0.05) among ratios (1:1, 2:1 and 1:2). However, the decrease in cell proliferation was the highest in black seed and honey mixture at 1:1 ratio (p<0.05) than individually treated black seed and honey. Thus, the black seed and honey mixture at ratio 1:1 was the most potent anticancer agent with an IC50 value of 7.44 μg/mL. The present study illustrated that black seed and honey mixtures possess a lipid-lowering effect via LDL-c uptake in HepG2 cells (p<0.05). The highest LDL-c uptake was observed at 15 μg/mL with the treatment of black seed and honey mixture at 1:2 ratio which was 294.4%. Further studies should be conducted on primary human liver cells to further justify the correlation between the antioxidant level and LDLc uptake mechanism of black seed and honey mixtures.

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