scholarly journals Widely Targeted Metabolomics Analysis Reveals Key Quality-Related Metabolites in Kernels of Sweet Corn

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Ruichun Yang ◽  
Yunfeng Li ◽  
Yuanyuan Zhang ◽  
Jun Huang ◽  
Junjie Liu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Sweet Corn ◽  
Quinic Acid ◽  
Essential Amino Acids ◽  
Free Form ◽  
Targeted Metabolomics ◽  
Widely Targeted Metabolomics ◽  
Corn Kernels ◽  
Metabolomics Analysis

Sweet corn (Zea mays convar. saccharata var. rugosa) is a major economic vegetable crop. Different sweet corn cultivars vary largely in flavor, texture, and nutrition. The present study performed widely targeted metabolomics analysis based on the HPLC-MS/MS technology to analyze the metabolic profiles in three sweet corn cultivars widely grown in China. A total of 568 metabolites in the three sweet corn cultivars were detected, of which 262 differential metabolites significantly changed among cultivars. Carbohydrates, organic acids, and amino acids were the majority detected primary metabolites. Organic acids were mainly concentrated on shikimate, benzoic acids, and quinic acid with aromatic groups. And the essential amino acids for the human body, methionine and threonine, were highly accumulated in the high-quality cultivar. In addition, phenylpropanoids and alkaloids were the most enriched secondary metabolites while terpenes were low-detected in sweet corn kernels. We found that the flavonoids exist in both free form and glycosylated form in sweet corn kernels. PCA and HCA revealed clear separations among the three sweet corn cultivars, suggesting distinctive metabolome profiles among three cultivars. The differential metabolites were mapped into flavonoid biosynthesis, phenylpropanoid biosynthesis, biosynthesis of amino acids, and other pathways according to the KEGG classification. Furthermore, we identified skimmin, N ′ ,N ″ -diferuloylspermidine, and 3-hydroxyanthranilic acid as the key quality-related metabolites related to grain quality traits in sweet corn. The results suggested variations of metabolic composition among the three cultivars, providing the reference quality-related metabolites for sweet corn breeding.

scholarly journals Correlation among Metabolic Changes in Tea Plant Camellia sinensis (L.) Shoots, Green Tea Quality and the Application of Cow Manure to Tea Plantation Soils

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6180
Author(s):  
Litao Sun ◽  
Kai Fan ◽  
Linlin Wang ◽  
Dexin Ma ◽  
Yu Wang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Organic Acids ◽  
Pearson Correlation ◽  
Late Summer ◽  
Quinic Acid ◽  
Tea Plant ◽  
Organic Manure ◽  
Gas Chromatography Mass Spectrometry ◽  
Cow Manure

Traditionally, the supplement of organic manure in tea plantations has been a common approach to improving soil fertility and promoting terroir compounds, as manifested by the coordinated increase in yield and quality for the resulting teas. However, information regarding the effect of organic manure in the metabolome of tea plants is still inadequate. The metabolite profiles of tea shoots applied with cow manure, urea or no fertilizer were studied using gas chromatography–mass spectrometry (GC–MS). In total, 73 metabolites were detected, and the modulated metabolites included mainly amino acids, organic acids and fatty acids. In particular, glutamine, quinic acid and proline accumulated more in tea shoots in soils treated with cow manure, but octadecanoic acid, hexadecanoic acid and eicosanoic acid were drastically reduced. Pearson correlation analysis indicated that organic acids and amino acids in tea shoots were the two major metabolite groups among the three treatments. The analysis of metabolic pathways demonstrated that the cow manure treatment significantly changed the enrichment of pathways related to amino acids, sugars and fatty acids. Sensory evaluation showed that the quality of green teas was higher when the plants used to make the tea were grown in soil treated with cow manure rather than urea during spring and late summer. The results indicated that the application of cow manure in soils changed the metabolic characteristics of tea shoots and improved the qualities of the resulting teas.

scholarly journals Widely Targeted Metabolomics Analysis to Reveal Transformation Mechanism of Cistanche Deserticola Active Compounds During Steaming and Drying Processes

2021 ◽  
Vol 8 ◽  
Author(s):  
Ziping Ai ◽  
Yue Zhang ◽  
Xingyi Li ◽  
Wenling Sun ◽  
Yanhong Liu
Keyword(s):  
Pathway Analysis ◽  
Thermal Processing ◽  
Fatty Acid Biosynthesis ◽  
Targeted Metabolomics ◽  
Transformation Mechanism ◽  
Metabolic Pathway Analysis ◽  
Active Compounds ◽  
Cistanche Deserticola ◽  
Widely Targeted Metabolomics ◽  
Metabolomics Analysis

Cistanche deserticola is one of the most precious plants, traditionally as Chinese medicine, and has recently been used in pharmaceutical and healthy food industries. Steaming and drying are two important steps in the processing of Cistanche deserticola. Unfortunately, a comprehensive understanding of the chemical composition changes of Cistanche deserticola during thermal processing is limited. In this study, ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based widely targeted metabolomics analysis was used to investigate the transformation mechanism of Cistanche deserticola active compounds during steaming and drying processes. A total of 776 metabolites were identified in Cistanche deserticola during thermal processing, among which, 77 metabolites were differentially regulated (p < 0.05) wherein 39 were upregulated (UR) and 38 were downregulated (DR). Forty-seven (17 UR, 30 DR) and 30 (22 UR, 8 DR) differential metabolites were identified during steaming and drying, respectively. The most variation of the chemicals was observed during the process of steaming. Metabolic pathway analysis indicated that phenylpropanoid, flavonoid biosynthesis, and alanine metabolism were observed during steaming, while glycine, serine, and threonine metabolism, thiamine metabolism, and unsaturated fatty acid biosynthesis were observed during drying. The possible mechanisms of the chemical alterations during thermal processing were also provided by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Furthermore, the blackening of the appearance of Cistanche deserticola mainly occurred in the steaming stage rather than the drying stage, which is associated with the metabolism of the amino acids. All results indicated that the formation of active compounds during the processing of Cistanche deserticola mainly occurred in the steaming stage.

Perturbations in amino acids and metabolic pathways in osteoarthritis patients determined by targeted metabolomics analysis

2018 ◽  
Vol 1085 ◽  
pp. 54-62 ◽  
Author(s):  
Rui Chen ◽  
Su Han ◽  
Xuefeng Liu ◽  
Kunpeng Wang ◽  
Yong Zhou ◽  
...  
Keyword(s):  
Amino Acids ◽  
Metabolic Pathways ◽  
Targeted Metabolomics ◽  
Metabolomics Analysis

Characterization of fatty acids, amino acids and organic acids in three colored quinoas based on untargeted and targeted metabolomics

LWT ◽  
2021 ◽  
Vol 140 ◽  
pp. 110690
Author(s):  
Jianxin Song ◽  
Yiming Yan ◽  
Xiaodong Wang ◽  
Xihong Li ◽  
Ye Chen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Organic Acids ◽  
Targeted Metabolomics
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