scholarly journals TOXICOLOGICAL SCREENING OF ELLAGIC ACID IN POMEGRANATE FRUIT AND HYDROXYAPATITE COMBINATION AS BONE GRAFT MATERIAL ON BHK-21 FIBROBLAST CELL

2020 ◽  
Vol 5 (1) ◽  
pp. 98
Author(s):  
Agung Satria Wardhana ◽  
Isyana Erlita ◽  
Intan Nirwana ◽  
Hendrik Setiabudi
Keyword(s):  
Bone Graft ◽  
Ellagic Acid ◽  
Alternative Therapy ◽  
Fibroblast Cell ◽  
Control Group ◽  
Graft Material ◽  
Control Group Design ◽  
Bone Graft Material ◽  
Post Test

Background: Bone graft is an alternative therapy for periodontitis and other bone destructive lesions. Several studies had revealed Ellagic Acid (EA) ability in increasing osteogenesis process. EA contains polyphenols, such as Ellagitannin, Gallotannin, and Anthocyanin, which demonstrate anti-inflammatory and antibacterial activity as well as growth factor stimulating effect. EA combination with bone graft material (hydroxyapatite) is anticipated to enhance bone osteogenesis yet no investigation was performed to identify its toxicity towards fibroblast cell. Objective: To analyze EA toxicity on fibroblast cell in vitro. Methods: This was a true experimental study using post-test only with control group design. Fibroblast cell was exposed with EA in eight different concentrations: 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5%. Control group comprised of cell control and media group. All groups were exposed to MTT Assay test and measured using Elisa Reader. Result: The calculation of cell viability value in EA groups at 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5% concentration were 88.2%, 92.3%, 97.5%, 89.5%, 84.2%, 90.7%, 88.9% and 89.4% respectively. Conclusion: All EA and hydroxyapatite combinations are not toxic towards BHK-21 fibroblast cells.

scholarly journals Histomorphometric Evaluation of Socket Preservation Using Autogenous Tooth Biomaterial and BM-MSC in Dogs

Scanning ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Jin-Hyun Kim ◽  
Puneet Wadhwa ◽  
HongXin Cai ◽  
Dong-Hyung Kim ◽  
Bing Cheng Zhao ◽  
...  
Keyword(s):  
Bone Graft ◽  
Alveolar Bone ◽  
Control Group ◽  
Ridge Preservation ◽  
Graft Material ◽  
Socket Preservation ◽  
Bone Graft Material ◽  
Premolar Extraction ◽  
Bone Width ◽  
Significant Difference

This study is aimed at assessing the dimensional alterations occurring in the alveolar bone after premolar extraction in dogs with histomorphometric and histological analysis. After atraumatic premolar extraction, tooth-derived bone graft material was grafted in the extraction socket of the premolar region in the lower jaws of six dogs in two experimental groups. In the second experimental group, BM-MSCs were added together with the graft. The control was left untreated on the opposite side. After twelve weeks, all six animals were sacrificed. Differences in alveolar bone height crests lingually and buccally, and alveolar bone width at 1, 3, and 5 mm infracrestally, were examined. Histologic study revealed osteoconductive properties of tooth biomaterial. A statistically significant difference was detected between the test and control groups. In the test groups, a reduced loss of vertical and horizontal alveolar bone dimensions compared with the control group was observed. Tooth bone graft material may be considered useful for alveolar ridge preservation after tooth extraction, as it could limit the natural bone resorption process.

scholarly journals UJI EFEK ANTI BAKTERI EKSTRAK BUNGA CENGKEH TERHADAP BAKTERI Streptococcus mutans SECARA IN VITRO

e-GIGI ◽  
2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Juvensius R. Andries ◽  
Paulina N. Gunawan ◽  
Aurelia Supit
Keyword(s):  
Streptococcus Mutans ◽  
Control Group ◽  
Antibacterial Effects ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Clove Extract ◽  
Post Hoc ◽  
One Way Anova

Abstrak: Minyak cengkeh berguna sebagai antibakteri alami. Minyak esensial dari cengkeh mempunyai fungsi anestetik dan antimikrobial. Zat yang terkandung dalam cengkeh yang bernama eugenol dapat membunuh bakteri termasuk bakteri yang resisten terhadap antibiotika, salah satunya adalah bakteri Streptococcus mutans. Bakteri ini merupakan mikroorganisme penyebab utama terjadinya karies. Tujuan dari penelitian ini yaitu untuk mengetahui efek antibakteri ekstrak cengkeh terhadap bakteri Streptococcus mutans secara in vitro. Penelitian ini merupakan penelitian eksperimental menggunakan post test only control group design. Penelitian ini menggunakan bahan coba ekstrak cengkeh dengan konsentrasi 40%, 60%, dan 80%, Ciprofloxacin, aquades dengan pengulangan sebanyak lima kali. Data dikumpulkan dan dianalisis dengan one-way ANOVA dan post-hoc uji LSD ( = 0,05). Berdasarkan hasil uji statistik penelitian uji efek antibakteri ekstrak cengkeh terhadap bakteri streptococcus mutans secara in vitro, dapat disimpulkaan bahwa ekstrak cengkeh memiliki efek antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans secara in vitro. Hasil uji lanjut post-hoc uji LSD menunjukan daya hambat ekstrak cengkeh 40%, 60%, 80%, lebih kecil (p<0,05) dalam menghambat Streptococcus mutans secara in vitro dibandingkan Ciprofloxacin. Kata Kunci: Ekstrak cengkeh, Streptococcus mutans.   Abstract: Clove oil is useful as a natural antibacterial agent, essential oil of clove has anesthetic and antimicrobial effect. Substances contained in clove called eugenol can kill bacteria including antibiotic resistant bacteria, one of which is the bacteria Streptococcus mutans. This bacteria is a major cause for caries. The purpose of this study was to mengetahui clove extrack antibacterial effects againts Streptococcus mutans bacteria in vitro. This study is an experimental study using a post test only control group design. This research try using clove extract with a concentration of 40%, 60%, and 80%, Ciprofloxacin, aquades repetition five times. Data collected and analyzed by one-way ANOVA and post-hoc LSD test (α = 0.05). Based on the results of the statistical test to test the effects of anti-bacterial research clove extracts against Streptococcus mutans bacteria in vitro, can disimpulkaan that clove extracts have antibacterial effects in inhibiting the growth of Streptococcus mutans bacteria in vitro.further test result post-hoc LSD test shoved its inhibitory clove extract 40%, 60%, 80% smaller (p<0,05)in hibiting Streptococcus mutans in vitro compared Ciprofloxacin. Keywords: clove extract, Streptococcus mutans

scholarly journals Hambat Ekstrak Etanol Rimpang Kunyit (Curcuma domestica V.) terhadap Pertumbuhan Bakteri Staphylococcus aureus Secara In Vitro

2018 ◽  
Vol 6 (3) ◽  
pp. 590
Author(s):  
Putri Ramadhani ◽  
Erly Erly ◽  
Asterina Asterina
Keyword(s):  
Staphylococcus Aureus ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Staphylococcus aureus merupakan salah satu bakteri patogen yang bisa menyebabkan infeksi. Penggunaan antibiotika untuk penanganan infeksi yang tidak rasional dapat membuat kuman patogen menjadi resistensi, sehingga penggunaan Rimpang kunyit (Curcuma domestica V.) mungkin dapat sebagai alternatif pengganti antibiotik. Tujuan penelitian ini adalah untuk mengetahui daya hambat ekstrak etanol rimpang kunyit (Curcuma domestica V.) terhadap pertumbuhan bakteri Staphylococcus aureus secara in vitro. Penelitian ini bersifat eksperimental laboratorium dengan desain post-test only control group design menggunakan metode difusi (cakram) yang dilakukan dari Februari 2015 sampai September 2015 di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Sampel yang digunakan adalah rimpang kunyit yang berasal dari ladang kunyit Puncak Payo, Tanah Garam Solok. Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak etanol rimpang kunyit (Curcuma domestica V.) memiliki daya hambat yang berbeda terhadap pertumbuhan bakteri S. aureus dengan berbagai konsentrasi yaitu 10%, 20%, 40%, 80% b/v . Konsentrasi ekstrak yang paling efektif dalam menghambat S. aureus adalah konsentrasi 80% b/v. Penggunaan ekstrak etanol rimpang kunyit (Curcuma domestica V.) sebagai alternatif pengganti antibiotik terhadap infeksi oleh S. aureus perlu dipertimbangkan.

scholarly journals PENGARUH KETEBALAN LAPISAN DENTIN TERHADAP KEKUATAN FLEKSURAL PADA GIGI TIRUAN CEKAT KERAMIK-LOGAM

2020 ◽  
Vol 15 (1) ◽  
pp. 46-51
Author(s):  
Sopan Sinamo ◽  
Susi Marlina Silalahi ◽  
Vivie Zahara
Keyword(s):  
Gold Standard ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

GTC keramik-logam adalah gigi tiruan cekat dengan bahan keramik-logam yang secara permanen disemenkan pada gigi geligi, dan sampai saat ini menjadi gold standard di Bidang Prostodontik, akan tetapi sering terjadi permasalahan pada GTC  keramik-logam antara lain fraktur adhesi dan fraktur kohesi. Penelitian ini bertujuan untuk mengetahui pengaruh ketebalan lapisan dentin terhadap kekuatan fleksural pada GTC keramik-logam. Metode penelitian yang digunakan adalah eksperimental laboratorium secara in vitro, dengan rancangan penelitian post  test only control group design. Jumlah sampel yang digunakan adalah sebanyak 27 sampel yang dibagi menjadi 3 kelompok, dimana masing-masing kelompok terdiri dari lapisan dentin 0,5mm, 0,6mm, dan 0,7mm. Hasil penelitian ini menunjukkan nilai rata-rata kekuatan fleksural pada GTC keramik-logam pada  ketebalan lapisan dentin 0,5 mm  adalah 95,32 MPa, 0,6 mm adalah 101,68 MPa, dan 0,7 mm adalah 104,30 MPa. Kesimpulan penelitian ini menunjukkan bahwa semakin tebal lapisan dentin maka dapat menghasilkan kekuatan fleksural yang optimal.

scholarly journals PENGARUH NIKOTIN TERHADAP KADAR MALONILDIALDEHID (MDA) SERUM DAN KEBERHASILAN FERTILISASI IN VITRO PADA RATTUS NOVERGICUS DI FK. UNAIR SURABAYA

EMBRIO ◽  
2012 ◽  
Vol 1 ◽  
pp. 1-5
Author(s):  
Sumiati Sumiati
Keyword(s):  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova

Penelitian ini untuk mempelajari pengaruh nikotin terhadap kadar MDA serum dan keberhasilan fertilisasi in vitro pada Rattus novergicus. Rancangan penelitian ini adalah eksperimental dengan post test only control group design. Subjek penelitian terdiri dari 4 kelompok (36 ekor tikus) yang dipilih secara acak dan homogen. Kelompok penelitian terdiri dari kelompok kontrol (K0), kelompok perlakuan yang diberikan injeksi nikotin subkutan selama 7 hari dengan dosis 70 mg/Kg BB (K1), 52,5 mg/kgBB (K2) dan 35 mg/kgBB (K3). Hasil yang diamati adalah adanya badan polar II/sigot 2 sel. Hasil uji one way Anova adalah terdapat perbedaan yang bermakna secara statistik (p<0,05), kemudian dilanjutkan dengan uji BNT. Hasil uji BNT kadar MDA serum adalah terdapat perbedaan yang bermakna (p<0,05) antara K0 dan K1, kelompok K0 dan K2, K1 dan K2, serta K1 dan K3. Angka keberhasilan fertilisasi tidak dapat dilakukan uji statistik karena seluruh data menunjukkan 100% berhasil. Dengan demikian tidak dapat dilakukan uji hubungan antara kadar MDA serum dengan angka keberhasilan fertilisasi. Kesimpulan penelitian ini adalah nikotin dapat menyebabkan peningkatan kadar MDA serum tapi belum dapat mempengaruhi fertilisasi sehingga sehingga tidak dapat menghubungkan antara kadar MDA serum dengan angka keberhasilan fertilisasi.

scholarly journals POTENSI AKTIVITAS ANTIMIKROBA MADU DAN HABBATUSSAUDA TERHADAP BAKTERI ESCHERICHIA COLI SECARA IN VITRO

2019 ◽  
Vol 2 (1) ◽  
pp. 11-19
Author(s):  
Alifia Ayu Delima ◽  
Utami Murti Pratiwi ◽  
Asriani
Keyword(s):  
Escherichia Coli ◽  
Control Group ◽  
Control Group Design ◽  
E Coli ◽  
Group Design ◽  
Post Test

Madu dan habbatussauda memiliki aktivitas antibakteri. Komponen yang terdapat di dalam madu antara lain keasaman, tekanan osmotik, dan hidrogen peroksida, asam aromatik serta omponen fenol juga berperan dalam aktivitas antibakteri. Sedangkan habbatussauda sendiri tannin, tymoquinon, thymol, a-pinene, p-cymene dengan cara menghambat pembentukan asam nukleat (RNA) dan sintesis protein yang berperan sebagai antibakteri dan antioksidan pada proses infeksi. Tujuan dari penelitian ini untuk mengetahui potensi antibakteri madu dan Habbatussauda terhadap bakteri Escherichia coli. Jenis penelitian ini adalah eksperimental dengan metode Post Test Only Control Group Design yang dilakukan secara in vitro. Hasil dari penelitian in vitro menunjukan bahwa terdapat pengaruh pemberian perlakuan madu, habbatussaudah dan kombinasi terhadap daya hambat pertumbuhan e-coli (<0.05).

Guided Tissue Regeneration of the Mixture of Human Tooth-Ash and Plaster of Paris in Dogs

2007 ◽  
Vol 330-332 ◽  
pp. 1327-1330 ◽  
Author(s):  
Ha Ra Ku ◽  
Hyun Seon Jang ◽  
S.G. Kim ◽  
Moon Jin Jeong ◽  
Joo Cheol Park ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Tissue Regeneration ◽  
Guided Tissue Regeneration ◽  
Human Tooth ◽  
Control Group ◽  
Graft Material ◽  
Bone Graft Material ◽  
Plaster Of Paris ◽  
Experimental Group

This study evaluated the efficacy of periodontal tissue regeneration using guided tissue regeneration (GTR) with a mixture of tooth ash and plaster as a bone substitute material in the treatment of class II furcation defects in dogs. GTR was performed at the site of a surgically formed mandibular premolar bifurcation area in dogs. Four adult dogs were used in the animal study. The animals were in a good periodontal state without any systemic disease. A resorbable membrane (Bio-Gide®, Swiss) and bone graft material using a mixture of tooth ash and plaster were used to examine the biological activity. The defect in the control group was filled a Bio-Gide® only. For the experimental group, the defects were filled with a mixture of tooth ash and plaster and covered by a Bio-Gide®. Oral rinsing with 0.12% chlorhexidine was performed twice a day for 2 weeks. In control group, new bone formation was observed adjacent to the preexisting bone. Soft tissue ingrowth was also noted. In experimental group, new lamellar type trabecular bone formation as well as centum and PDL regeneration was observed in the base of the infrabony pocket. More bone regeneration would be expected when GTR is performed using a mixture of human tooth-ash and plaster of Paris as a bone graft material.

scholarly journals EFEK ANTIBAKTERI SUPERNATAN JUS ANGGUR MERAH YANG DIISOLASI DENGAN KECEPATAN SENTRIFUGASI 12.000 rpm TERHADAP PERTUMBUHAN S. MUTANS

2016 ◽  
Vol 3 (2) ◽  
pp. 81
Author(s):  
Dini Rachmawati ◽  
Sumarno . ◽  
Anggi Wahyu Nur Cahyani
Keyword(s):  
Streptococcus Mutans ◽  
Brain Heart Infusion ◽  
Grape Juice ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Post Test ◽  
Red Grape Juice ◽  
Red Grape

Background: Streptococcus mutans (S.mutans) is a gram-positive bacteria in oral cavity which produced acid as the common cause of dental caries. In order to prevent the growth or kill Streptococcus mutans, antibacterial is required. Red grape (Vitis vinifera) is suspected to contain antibacterial.Method: An experimental study using the post test only control group design is carried out to Streptococcus mutans with tube dilution method and streaking on the Brain Heart Infusion. A range of concentration from 40%, 50%, 60%, 70%, 80%, 90%, 98%. The control group are group of bacteria and red grape juice control.Result: The analysis of Minimum Inhibitor Bactericidal (MIC) is show a difference absorbent value as the given concentration (Anova, p<0,05). The correlation test show is strong reciprocal association between concentration of res grape juice and absorbent value (r=-0,978; p< 0,05). The analysis of Minimum Bactericidal Concentration (MBC) is shows difference of the number of colonies as given concentration (Anova, p<0,05). The correlation reveals a very strong reciprocal association between concentration of red grape juice and the number of Streptococcus mutans colonies growth (r=-0,925; p< 0,05).Conclusion: The conclusion is supernatant of red grape juice has effect the growth as antibacterial agent on Streptococcus mutans in vitro, with MIC at concentration 60% and MBC at concentration 80%

scholarly journals EFEKTIFITAS EKSTRAK ETANOL UMBI SARANG SEMUT (Hydnophytum formicarum) DALAM PEMBENTUKAN ZONA HAMBAT TERHADAP PERTUMBUHAN BAKTERI Porphyromonas sp SECARA IN VITRO

2019 ◽  
Vol 4 (1) ◽  
pp. 61-66
Author(s):  
Sarah Putri Utami ◽  
Efa Ismardianita ◽  
Andries Pascawinata
Keyword(s):  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova

Porphyromonas sp adalah bakteri Gram-negatif anaerob yang banyak ditemui di membran mukosa dan sering menyebabkan infeksi piogenik seperti infeksi mulut, gigi, saluran akar dan gingiva bila bereaksi bersamaan dengan beberapa bakteri. Pengobatan dengan obat-obatan herbal merupakan salah satu alternatif untuk mengganti obat-obatan dengan bahan dasar kimia. Tujuan penelitian ini adalah untuk mengetahui efektivitas ekstrak etanol umbi sarang semut (Hydnophytum formicarum) dalam menghambat pertumbuhan bakteri Porphyromonas sp, jenis penelitian yang digunakan adalah penelitian eksperimental laboratorium dengan rancangan penelitian post test only control group design, penelitian dilakukan pada bulan April 2017, besar sampel yang digunakan adalah 40 sampel dengan 8 konsentrasi yang terdiri dari 2 kelompok perlakuan dengan pengulangan sebanyak 4 kali. Penelitian ini menggunakan 8 konsentrasi dosis konsentrasi ekstrak 0.78%, 1.56%, 3.12%, 6.25%, 12.5%, 25%, 50% dan 100% serta kontrol negatif (aquades steril) dan kontrol positif (Metronidazole) dengan menggunakan uji one way anova. Hasil penelitian menunjukan bahwa ekstrak etanol umbi sarang semut (Hydnophytum formicarum) efektif terhadap zona hambat pertumbuhan bakteri Porphyromonas sp dengan ekstrak etanol umbi sarang semut (Hydnophytum formicarum) yang paling efektif terhadap zona hambat adalah pada ekstrak 6,25%.

scholarly journals PERBANDINGAN EFEKTIVITAS MADU ALAMI DENGAN MADU KEMASAN TERHADAP PEMUTIHAN GIGI SECARA IN VITRO

2014 ◽  
Vol 2 (2) ◽  
pp. 105-113
Author(s):  
Wilda Sahroni ◽  
Elizabeth Bahar ◽  
Dedi Sumantri
Keyword(s):  
Colour Change ◽  
Control Group ◽  
Control Group Design ◽  
Dental Bleaching ◽  
Tooth Discoloration ◽  
Before And After ◽  
Post Test ◽  
Raw Honey ◽  
Natural Honey

Discoloration of teeth might be overcome by dental bleaching treatment. But the use of chemical bleaching agents caused a side effect. Therefore, many researchers were looking for alternative materials made from nature which is safer to used. Honey devided into 2 types, natural raw honey and packed honey, which both consist hydrogen peroxide (H2O2) that could whiten the teeeth. The purpose of this study was to compare the effectiveness of natural honey and packed honey for dental bleaching. This research was an in vitro experimental study with pre and post test control group design. 30 upper human premolar were used, devided into 3 groups based on bleaching agents used: 10 samples each group with application of natural honey, packed honey, and 10% carbamid peroxide. After application, samples were placed in incubator in 370C for 2 hours, then washed and soaked in artificiaal saliva, and incubated for 14 days. The colour change was observed by five observers using vitapan classical shade guide. The results of Kruskal-Wallis test showed p=0,000 witg significant value <0,05. Which means there was significant changes between before and after treatment. The average of tooth discoloration of 50% natural honey was 8,50, 50% packed honey was 7,30, and 10% carbamid peroxide as the contro group showed 10,10. In conclusion, natural honey was more effective in tooth whitening than packed honey.

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