Reversal of Radiotherapy Resistance of Ovarian Cancer Cell Strain CAOV3/R by Targeting lncRNA CRNDE

Radiotherapy resistance is one of the key factors of poor prognosis of ovarian cancer clinical treatment. The search for key targets of ovarian cancer radiotherapy resistance has become a high priority. Long noncoding RNA plays an important role in tumor development. However, the key lncRNA in ovarian cancer radiotherapy resistance is not identified. Our finding that lncRNA CRNDE is highly expressed in the radiotherapy resistance cell line CAOV3/R drew our attention. Therefore, in this study, we targeted lncRNA CRNDE to analyze whether it is a key factor of radiotherapy resistance in ovarian cancer. Ultimately, we found that silencing lncRNA CRNDE could reverse CAOV3/R radiotherapy resistance, which would be a boon to clinical treatment.

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ELNCRNA1, A LONG NONCODING RNA TRANSCRIPTIONALLY INDUCED BY OESTROGEN, PROMOTES EPITHELIAL OVARIAN CANCER CELL PROLIFERATION

10.26226/morressier.599bdc7ad462b80296ca15fa ◽

2017 ◽

Author(s):

junjun qiu

Keyword(s):

Ovarian Cancer ◽

Cell Proliferation ◽

Epithelial Ovarian Cancer ◽

Cancer Cell ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Ovarian Cancer Cell ◽

Cancer Cell Proliferation ◽

Epithelial Ovarian Cancer Cell

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Overexpression of KIF2A is Suppressed by miR-206 and Associated with Poor Prognosis in Ovarian Cancer

Cellular Physiology and Biochemistry ◽

10.1159/000494467 ◽

2018 ◽

Vol 50 (3) ◽

pp. 810-822 ◽

Cited By ~ 16

Author(s):

Nan Sheng ◽

Yun-Zhao Xu ◽

Qing-Hua Xi ◽

Hai-Yan Jiang ◽

Chen-Yi Wang ◽

...

Keyword(s):

Ovarian Cancer ◽

Cell Proliferation ◽

Cancer Cell ◽

Poor Prognosis ◽

Ovarian Cancer Cell ◽

Expression Profiles ◽

Annexin V ◽

Prognostic Indicator ◽

Luciferase Reporter ◽

Migration And Invasion

Background/Aims: This study aimed to investigate the expression and prognostic value of kinesin family member 2A (KIF2A) and the suppression effects of microRNA-206 (miR-206) on KIF2A in ovarian cancer. Methods: Ovarian cancer tissues from patients and ovarian cancer cell lines (A2780 and SKOV3) were used in this study. miR-206 mimics and control were transiently transfected into cells. RT-qPCR was performed to detect KIF2A mRNA and miR-206 expression levels, Western blot was performed to detect KIF2A protein levels, Dual-Luciferase Reporter Assay was used to examine the inhibition effects of miR-206 on KIF2A mRNA, immunohistochemical staining was used to examine the expression of KIF2A in tissue sections. CCK-8, transwell and Annexin-V-FITC/Propidium Iodide staining with flow cytometry were used to detect the cell proliferation, migration/invasion, and apoptosis respectively. Results: Our study explored the expression profiles of KIF2A and miR-206 in the patients with ovarian cancer. We found that overexpression of KIF2A was associated with a poor prognosis in ovarian cancer. We also found that KIF2A mRNA contains two target sites for miR-206 binding and confirmed that miR-206 directly suppresses KIF2A; inhibits ovarian cancer cell proliferation, migration, and invasion; and induces apoptosis. Conclusion: The results suggest KIF2A could serve a valuable prognostic indicator in ovarian cancer and provide a rationale for treatment of ovarian cancer by targeting KIF2A via miR-206.

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